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Effects of Natural Product on the Inhibition of $5{\alpha}-Reductase$ Type 2 for the Development of Chemopreventive Agents in LNCaP Cells

  • Lee, Sung-Jin;Kim, Kyeong-Ho;Cho, Myung-Haing;Lee, Sang-Kook;Mar, Woong-Chon
    • Natural Product Sciences
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    • v.5 no.2
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    • pp.97-103
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    • 1999
  • The enzyme steroid $5{\alpha}-reductase$ is responsible for the conversion of testosterone into the most potent androgen dihydrotestosterone (DHT). In man, this steroid acts on a variety of androgen-responsive target tissues to mediate such diverse endocrine processes as male sexual differentiation in the fetus and prostatic growth in men. Androgen levels in the prostate may influence carcinogenesis in this organ. The use of a $5{\alpha}-reductase$ inhibitor, finasteride, in the chemoprevention of prostate cancer is being evaluated in a clinical trial and have been used successfully for treatment of benign prostatic hyperplasia. Therefore, for the discovery of $5{\alpha}-reductase$ type 2 inhibitors, we have evaluated the inhibitory effects of solvent fractionated extracts of natural products on $5{\alpha}-reductase$ type 2 activity. We have tested approximately 80 kinds of natural products after partition into n-hexane, ethyl acetate and aqueous layers from 100% methanol extracts of plants. The ethyl acetate fractions of Perilla sikokiana $(seed,\;IC_{50}\;:\;6.2\;ug/ml)$, Sophora flavescens $(root,\;IC_{50}\;:\;8.9\;ug/ml)$, and Angelica tenuissima $(root,\;IC_{50}\;:\;11.7\;ug/ml)$ revealed inhibitory effects on $5{\alpha}-reductase$ 2 activity in LNCaP cells. The effective ethyl acetate fractions of Perilla sikokiana, Sophora flavescens, Hydnocarpus anthelmintica, and Angelica tenuissima were subfractionated by column chromatography and tested. The subfractions $F4\;(IC_{50}\;:\;1.1\;ug/ml),\;F5\;(IC_{50}\;:\;2.0\;ug/ml),\;and\;F6\;(IC_{50}\;:\;5.8\;ug/ml)$ of the ethyl acetate fraction of Perilla sikokiana and the subfraction $F8\;(IC_{50}\;:\;5.3\;ug/ml)$ of the ethyl acetate fraction of Sophora flavescens displayed greater inhibition of $5{\alpha}-reductase$ type 2 than did finasteride in LNCaP cells. These active fractions are under the process of further sequential fractionation to find the effective pure compounds against $5{\alpha}-reductase$ 2 activity.

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Effect of Chromium Dietary Supplementation on the Immune Response and Some Blood Biochemical Parameters of Transport-stressed Lambs

  • Al-Mufarrej, S.I.;Al-Haidary, I.A.;Al-Kraidees, M.S.;Hussein, M.F.;Metwally, H.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.5
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    • pp.671-676
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    • 2008
  • Forty-eight Naemi lambs (avg. BW 31.7 kg) were transported by truck for a distance of 1,450 km from Al-Jouf to Riyadh, Saudi Arabia. On arrival day, the lambs were randomly allocated to four groups receiving diets supplemented with 0.0, 0.3, 0.6 and 0.9 ppm organic chromium (Cr). Each group consisted of four separately housed replicates of three lambs each. The animals were fed ad libitum on a grower diet for 84 days. Blood samples were obtained shortly before transportation, upon arrival and at weekly intervals thereafter from all lambs for analysis of plasma and serum. Plasma glucose and serum cortisol, total protein, albumin, urea-N and total cholesterol concentrations were determined. A cursory clinical examination of the lambs, along with rectal temperature, was undertaken at different intervals during the experiment. The lambs were inoculated each with 2 ml i.v. chicken red blood cells (CRBC) on days 0, 21, and 42. Serum total, IgG and IgM antibody titers were determined at weekly intervals post-immunization. An in vivo intradermal hypersensitivity test was carried out on 6 lambs from each group on days 10 and 70. Transportation of the lambs resulted in a significant (p<0.001) elevation of serum cortisol, total protein and albumin levels, as well as increased plasma glucose concentration, with corresponding decrease in total cholesterol, while blood urea-N remained largely unchanged. These constituents returned to normal levels during subsequent weeks, with no significant differences in their concentrations being observed between the Cr-supplemented groups and controls. Rise in rectal temperature after transportation was reduced to a greater extent (p<0.05) in Cr-supplemented versus control lambs. Total, IgG and IgM antibody titers against CRBC rose significantly (p<0.05) during immunizations in all groups, with significantly and linearly higher (p<0.05) total and IgG titers in Cr-supplemented versus control lambs. By contrast, no significant effect due to Cr supplementation was recorded in IgG titers, which increased equally in Cr-fed and control groups. Skin thickness in response to intradermal inoculation of phytohaemagglutinin (PHA) was also significantly (p<0.01) increased as a result of Cr supplementation. These results indicate that dietary Cr supplementation might be useful during stress especially for enhancing immune responses in transport-stressed lambs.

Evaluation of CO2 Storage and Uptake by Forest Landscapes in the Middle Region of Korea

  • Jo, Hyun-Kil;Ahn, Tae-Won
    • Journal of Environmental Science International
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    • v.22 no.2
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    • pp.139-149
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    • 2013
  • Anthropogenic increases in greenhouse gas concentrations, primarily through radiative forcing from carbon dioxide, continue to challenge earth's climate. This study quantified $CO_2$ storage and uptake by dominant forest types and age classes in the middle region of Korea. In addition, the role of forest landscapes in reducing atmospheric $CO_2$ against $CO_2$ emissions based on energy consumption was evaluated. Mean $CO_2$ storage and uptake per unit area by woody plants for three forest types and four age classes were estimated applying regression equations derived to quantify $CO_2$ storage and uptake per tree; and computations per soil unit area were also performed. Total $CO_2$ storage and uptake by forest landscapes were estimated by extrapolating $CO_2$ storage and uptake per unit area. Results indicated mean $CO_2$ storage per unit area by woody plants and soils was higher in older age classes for the same forest types, and higher in broadleaved than coniferous forests for the same age classes, with the exception of age class II (11-20 years). $CO_2$ storage by broadleaved forests of age class V (41-50 years) averaged 662.0 t/ha (US$331.0 hundred/ha), highest for all forest types and age classes evaluated. Overall, an increased mean $CO_2$ uptake per unit area by woody plants was evident for older age classes for the same forest types. However, decreased $CO_2$ uptake by broadleaved forests at age class V was observed, compared to classes III and IV with an average of 27.9 t/ha/yr (US$14.0 hundred/ha/yr). Total $CO_2$ storage by woody plants and soils in the study area was equivalent to 3.4 times the annual $CO_2$ emissions, and woody plants annually offset the $CO_2$ emissions by 17.7%. The important roles of plants and soils were associated with 39.1% of total forest area in South Korea, and $CO_2$ emissions comprised 62.2% of the total population. Therefore, development of forest lands may change $CO_2$ sinks into sources. Forest landscape management strategies were explored to maintain or improve forest roles in reducing atmospheric $CO_2$ levels.

A Study on Karl Foerster's Garden from the Viewpoint of Planting Design (재식설계의 관점에서 본 칼 푀르스터(Karl Foerster)의 정원 연구)

  • Park, Eun-Yeong
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.31 no.3
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    • pp.38-44
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    • 2013
  • Karl Foerster had the most significant influence on the establishment of the German garden culture. From the viewpoint of planting design, in particular, he greatly contributed to the selection of new materials and the distribution of gardens. Therefore it is worth re-exploring his garden in that he triggered the use of natural planting that is widely used these days and already used ornamental grasses in his garden designs. This study aimed to review Foerster's garden to understand his planting design methods and their historical significance. The results suggest: Firstly, in spatial structure he designed sunken gardens influenced by the climate and triggered the use of natural planting by applying naturally open meadow, ferns and grasses. Secondly, in terms of the use of materials, he pointed out the necessity to use perennial plants. He bred perennial plants that the general public could easily grow and that were resistant against cold and created show gardens to demonstrate the characteristics of the plants and possible combination methods to the public. Thirdly, in terms of the aesthetical principles of his planting design, he focuses on colors and seasons and was interested in blue, yellow and white colors. In doing so, he developed new varieties such as Delphinium, Rudbeckia and Phlox. He emphasized the necessity of seven seasons, which served as the ancestor of contemporary winter gardens.

A Study on the Current Situation and Resolution System of Labor Dispute in China (중국의 노동쟁의 현황 및 처리제도에 관한 연구)

  • Ha, Hyun-Soo
    • Journal of Arbitration Studies
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    • v.20 no.3
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    • pp.93-120
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    • 2010
  • In 1978, Chinese reform and opening caused a big changes in Chinese labor relationship. Through reforming and opening, China gave up part of state ownership system and group ownership system, permitted private ownership system, and also opened the way for capitalists to ride again. Since China was established, the labor relationship ceased for 30 years has been appeared. However because the top priority aim of China's reform was economic growth, the protection of the rights and interests of labor was pushed back on the policy priority list. China takes foreign capitals based on cheap labor force quickly and China come up the worldwide plants. Since reformed, China keeps an economic growth rate of 9.7% annually for 30years. This economic growth is based on labor's sacrifice. However, Chinese fast economic growth causes side effects such as increasement of the gap between the wealthy and the poor, increasement of unbalanced development between regions, and the increasement of conflict between labor and management. Especially, according to changes in labors' level of consciousness, the labors recognized that their rights and interests are exploited by employers. Therefore, the labor dispute is continuously increasing. Chinese government changes their policy from the policy focusing on enterprise development to the policy protecting labor's rights and interests. In order to protect labor's rights and interests, China conducts labor contract law and labor dispute conciliation arbitration law in 2008. This kind of changes in Chinese labor environment affect a lot to Korean companies which already entered into China or are willing to enter. According to studying on present situation and resolution system in Chinese labor dispute, this paper suggests the proper countermeasure related to labor dispute of Korean companies which entered in China. First, the success rate of labor dispute conciliation by enterprise labor dispute conciliation committee is around 20% during recent several years and the success rate by year is in decline. Therefore, when labor dispute is occurred, our companies which entered into China better use other labor dispute methods such as negotiation and arbitration than conciliation in order to settle a conflict. Second, from the Korean enterprises entered in China point of view, there exists a problem not to sue except special cases which provided in the law even though they are dissatisfied with arbitrate judgment. Thus, when labor dispute occurred, Korean enterprises try to do best to settle the dispute through negotiation. However, in case of that the dispute cannot be settled by negotiation, they have to attend in the arbitration as if it is a last chance. Third, Korean enterprises keep in mind that dispute handling procedures between labor union and users or between labor group and users are different, and then deal with separately. Thus, dispute between labor and users have to follow arbitrate procedures as a necessary procedure, but in case of dispute related to group contract, namely dispute against labor union, labor dispute can be settled by arbitrate or suit, so after figuring out the situation exactly, it is necessary to select more advantageous way in order to settle the dispute. Moreover, in case of the dispute between labor union, they have to keep in mind that conciliation procedures cannot be used.

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Growth Performance and Antibody Response of Broiler Chicks Fed Yeast Derived β-Glucan and Single-strain Probiotics

  • An, B.K.;Cho, B.L.;You, S.J.;Paik, H.D.;Chang, H.I.;Kim, S.W.;Yun, C.W.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.7
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    • pp.1027-1032
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    • 2008
  • A study was conducted to evaluate the effects of dietary yeast derived ${\beta}$-glucan and single-strain probiotics on the growth performance and antibody response in broiler chicks. Six hundred and thirty 1-d-old male broiler chicks were divided into seven groups, placed into three pens per group (30 birds per pen) and fed one of seven non-medicated corn-SBM based experimental diets containing 0.025, 0.05 or 0.1% Saccharomyces cerevisiae ${\beta}$-glucan and 0.05, 0.1 or 0.2% Bacillus amyloliquefaciens (BA-pro, $1.3{\times}10^9/g$) or devoid of them for 5 wk. The body weight gains in groups fed diets containing 0.025 or 0.1% ${\beta}$-glucan, 0.1% or 0.2% BA-pro were significantly higher (p<0.05) than the control over 1-35 d. Feed conversion rates of groups fed ${\beta}$-glucan and BA-pro tended to be improved compared to the control group. There were no significant differences in the relative weights of liver, abdominal fat and breast muscle. No significant differences were observed in the activities of serum enzymes and concentrations of various cholesterol fractions. The antibody titers against Newcastle disease or infectious bronchitis virus in the chicks fed diets containing ${\beta}$-glucan and BA-pro were significantly higher (p<0.05) than in the control. The concentrations of cecal lactic acid bacteria in all groups fed BA-pro were significantly increased (p<0.05) compared to the control. These results indicated that dietary yeast derived ${\beta}$-glucan and BA-pro exerted growth-promoting and immune-enhancing effects in broiler chickens. In addition, BA-pro added to the diets modulated the profiles of cecal microflora, reflecting a potential to be beneficial microorganisms in chickens.

Epidemiologic Characteristics of Carbon Monoxide Poisoning: Emergency Department Based Injury In-depth Surveillance of Twenty Hospitals (일산화탄소 중독의 역학적 특징: 전국 20개 병원 응급실 손상환자 표본 심층조사)

  • Bae, Sohyun;Lee, Jisook;Kim, Kyunghwan;Park, Junseok;Shin, Dongwun;Kim, Hyunjong;Park, Joonmin;Kim, Hoon;Jeon, Woochan
    • Journal of The Korean Society of Clinical Toxicology
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    • v.14 no.2
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    • pp.122-128
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    • 2016
  • Purpose: This study was conducted to describe the characteristics of patients with carbon monoxide (CO) poisoning. Methods: We retrospectively surveyed data from the Emergency Department based Injury In-depth Surveillance of 20 hospitals (2011-2014). We included patients whose mechanism of injury was acute CO poisoning caused by inhalation of gases from charcoal or briquettes. We surveyed the annual frequency, gender, age, result of emergency treatment, rate of intensive care unit (ICU) admission, result of admission, association with alcohol, and place of accident. We also surveyed the cause and experience of past suicide attempts by intentional poisoning. Results: A total of 3,405 patients were included (2,015 (59.2%) and 1,390 (40.8%) males and females, respectively) with a mean age of $39.83{\pm}18.51$ year old. The results revealed that the annual frequency of CO poisoning had increased and the frequency of unintentional CO poisoning was higher than that of intentional CO poisoning in January, February and December. The mean age of intentional CO poisoning was younger than that of unintentional CO poisoning ($38.41{\pm}13.03$ vs $40.95{\pm}21.83$) (p<0.001). The rates of discharge against medical advice (DAMA), ICU care and alcohol association for intentional CO poisoning were higher than for unintentional CO poisoning (36.4% vs 14.0%, 17.8% vs 4.7%, 45.2% vs 5.6%) (p<0.001). The most common place of CO poisoning was in one's residence. Conclusion: The annual frequency of total CO poisoning has increased, and unintentional CO poisoning showed seasonal variation. DAMA, ICU care, and alcohol association of intentional CO poisoning were higher than those of unintentional CO poisoning.

Comparative Phenolic Composition and Antioxidant Properties of Honey and Honeycomb Extracts (벌꿀과 벌집채꿀의 페놀성분 및 항산화 효과 비교)

  • Kang, Da Hee;Kim, Min Young
    • Journal of Life Science
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    • v.25 no.10
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    • pp.1169-1175
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    • 2015
  • Although many studies have described the physiological effects of bee products, such as honey, propolis, pollen, and royal jelly, the health benefits of honeycomb remain incompletely characterized. We performed a comparative study of the antioxidant properties of honey and honeycomb extracts using two different solvents (water and 95% ethanol). The results showed that the total phenolic and flavonoid content of the honeycomb extract was higher than that of the honey extract. They also demonstrated that water was more effective than ethanol in extracting total phenols. The in vitro antioxidant properties of the water honeycomb extract were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) radical scavenging assays and ferrous ion chelating and reducing power assays. The antioxidant activity of the honeycomb extract exhibited was higher than that of the honey extract. The 50% effective concentrations (EC50) of the honeycomb extract were 7.3±0.26 mg/ml for scavenging DPPH radicals, 6.1±0.22 mg/ml for scavenging NO radicals, 6.9±0.44 mg/ml for chelating ferrous ions, and 8.2±0.11 mg/ml for reducing power. A correlation analysis revealed that the total phenolics and flavonoids of the honeycomb extract were the major contributors to the radical scavenging activity, ferrous ion chelating, and reducing power. The honeycomb extract was effective in protecting biological systems against various oxidative stresses in vitro. This is the first report on the antioxidant properties of honeycomb.

Effects of Fructus Foeniculi extract on recovering liver function (회향의 간장기능 회복효과)

  • Lee, Jang-Cheon;Lee, Eun;Oh, Hwang;Yoon, Ho-Suck;Ha, Tae-Kwang;Hong, Eun-Hee;Lee, Young-Cheol
    • The Korea Journal of Herbology
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    • v.22 no.4
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    • pp.213-218
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    • 2007
  • Objectives : Effects of Fructus Foeniculi extract on liver function were investigated in carbon tetrachloride(CCl4) intoxicated rats. Methods : Thirty two male Sprague-Dawley rats with mean weight of $227.28{\pm}7.92g$ were used in these experiments and housed with food and water ad libitum. Fructus Foeniculi extract was administerd at dose 100mg/kg/day and 200mg/kg/day p.o. for 2 weeks after that CCl4 was treated 3 times at dose of 2.5ml/kg, p.o. in alternate day basis. Then serum AFP(${\alpha}$-Fetoprotein), Total protein, Albumin, Triglyceride, Total cholesterol concentrations and ALP (Alkaline phosphatase), AST(Aspartate Aminotransferase), ALT(Alanine Aminotransferase), ${\gamma}$-GT( ${\gamma}$-Glutamyl transferase), LDH(Lactate Dehydrogenase) activities were determined with commercial kit by autoanalyzer. Results : Plasma ${\alpha}$-fetoprotein and total protein concentration showed a tendency to decrease in Fructus Foeniculi extract-treated groups. However, plasma albumin concentration showed no significant differences in all treatment groups. Activity of plasma aspartate aminotransferase and alanine aminotransferase in Fructus Foeniculi extract-treated groups showed a lower value than that of control group. Alkaline phosphatase and lactate dehydrogenase activities showed a tendency to decrease in Fructus Foeniculi treated groups. However, ${\gamma}$-glutamyl transferase activity showed no significant difference in all treated groups. Concentration of plasma triglyceride and total cholesterol showed a high level in CCl4 intoxicated rats but not in Fructus Foeniculi treated groups. Conclusion : Reviewing these experimental results, it appears that Fructus Foeniculi extract have recovering effect against liver injury.

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Effects of Galgeunhaegitang-gamibang Administration along with Samhwangseze-gamibang on Atopic Dermatitis of NC/Nga Mice (갈근해기탕가미방(葛根解肌湯加味方)과 삼황세제가미방(三黃洗劑加味方) 병용이 NC/Nga 생쥐의 아토피 피부염에 미치는 영향)

  • Hwang, Chi-Hwan;Yun, Chae-Sung;Song, Seung-Hyeon;Weon, Young-Ho;Hwang, Chung-Yeon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.21 no.2
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    • pp.1-18
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    • 2008
  • Objectives: Galgeunhaegitang-gamibang(GH) and Samhwangseze-gamibang(SG) has been known that they are helpful for treatment of atopic dermititis clinically, but there is no report about the effect of GH and SG. So, author aimed to investigate the effects of GH and SG on atopic dermititis of NC/Nga mice. Methods : NC/Nga mice were divide into three group : normal, control, and experimental group. Atopic dermatitis was induced in the control and experimental group by spreading DNCB. Then GH was orally administered three times in a week for 8 weeks to the experimental group and SG was spreaded two times in a day for 8 weeks to the experimental group, while the control group was given normal saline. We observed changes of clinical skin severity score, serum IgE, IL-4, IL-4, IL-5, IL-6, IgM, IgGl, $IFN-{\gamma}$ and so on. We used one-way ANOVA test statistically(p<0.01). Results : Clinical skin severities of experiment group in 13 and 16weeks were significantly decreased by 48% and 55% compared to the control group. Serum IgE, IL-4, IL-5, IL-6, IgM, IgGl levels of experimental group were singnificantly decreased compared to the control group. Serum $IFN-{\gamma}$ level of the experimental group was significantly increased against control group. mRNA expression levels of IL-4, IL-5, IL-6 and CCR3 in the skin tissues of experimental group were significantly decreased compared to the control group. In contrary, $IFN-{\gamma}$y mRNA expression level were increased compared to the control group. Histological observation of the ear and skin tissues showed that the extents of inflammation and infiltrated immune cells in the epidermis and dermis of experimental group were highly deminished compared to the control group. Judging from that $IL-1{\beta}$, $TNF-{\alpha}$, IL-6 expression of gene, the effects of inflammatory cytokine revelation were significantly decreased compared to the control group. In the model inducing COX-2 activity in RAW 264.7 cell, COX-2 activity was significantly inhibited depending on the density of GH compared to the control serum. According to cell multiplication, examination of cell toxicity showed that GH is safe at the density of 10, 50, 100mg/l and even 1000mg/l. Conclusion : Accordin to the above results, it is considered that GH and SG is effective treatment for the atopic dermatitis.

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