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CHLOROPHYLL FLUORESCENCE IN CUCUMBER (Cucumis safivus L.) AND PEA (Pisum sativum L.) LEAVES UNDER CHILLING STRESS IN THE LIGHT AND DURING THE SUBSEQUENT RECOVERY PERIOD

  • Ha, Suk-Bong;Eu, Young-Jae;Lee, Choon-Hwan
    • Journal of Photoscience
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    • v.3 no.1
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    • pp.15-21
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    • 1996
  • To investigate the chilling sensitivity related injuries in the photosynthetic apparatus of cucumber leaves, the light-chilling induced alterations of chlorophyll fluorescence transients in cucumber leaves were compared with those in pea leaves. As an early effect of light-chilling, an increase in Fp/Fm$^*$ was observed in both pea and cucumber leaves, which was saturated by about 6 h chilling. However, the saturated value of Fp/Fm was almost 1.0 in cucumber, in contrast to about 0.8 in pea. During the recovery period after 24 h chilling, the light-chilling induced changes in pea seemed to be reversed, but those in cucumber leaves were thought to be irreversible, because Fo was increased significantly. Light-chilling caused significant decreases in qQ and qE in cucumber leaves, but qR was increased until 6 h, and decreased thereafter. In both pea and cucumber leaves, Fm was increased by 2 h dark treatment. The Fm from the predarkened pea leaf discs was higher than the value from the preilluminated ones during the whole period of light-chilling (500 $\mu$mol m$^{-2}$s$^{-1}$ PAR). However, the predarkened cucumber leaf discs showed a reduction in Fm and an increase in Fo during the 2 h chilling in the light. These results indicate that the causes of chilling sensitivities in photosynthetic apparatus of cucumber leaves are possibly related with the damage in PSI reaction center and the ability of acidification of lumen by PSII.

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The Effect of Redox Potential on the Kinetics of Lysine Production by Corynebacterium glutamicum (Corynebacterium glutamicum에 의한 Lysine 생산에 있어서 산화환원 전위가 발효속도론적 특성에 미치는 영향)

  • 이진희;김성준;이재흥
    • Microbiology and Biotechnology Letters
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    • v.19 no.1
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    • pp.76-81
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    • 1991
  • - The effect of redox potential (ORP) on lysine production by a leucine auxotrophic regulatory mutant of Corynebacterium glutclmicum on molasses medium was investigated in a 2-1 jar fermentor at pH 6.9 and $32^{\circ}C$. At a dilution rate of D=O.l $h ^1$, a maximum yield of Yr,,s=0.24 was obtained in either carbon- or leucine-limited chemostat where the redox potential was between -60 mV and - 100 mV. This level of redox potential corresponded to moderate oxygen deficiency. Under a high oxygen deficient condition of the redox potential of - 130 rnV (oxygen-limited chemostat), all the kinetic parameters such as $Y_[p/s}, q_s\; and \; q_p$ were decreased significantly and significant amounts of byproducts including glycine, alanine and valine were accumulated in the culture, indicating that the control of redox potential is important in lysine fermentation. At the redox potential of - 40 mV, on the other hand, large quantities of arginine (up to 0.38g/l) and glutamic acid (up to 0.12 g/l) were produced. A maximum lysine productivity of 2.41 g/l/h was achieved at - 66 mV under a carbon-limited condition.

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An Analysis of Construction Execution Tasks for Successful Residential Mechanical Projects (프로젝트 성공을 위한 효과적인 시공업무 및 영향 분석 - 아파트 설비공사를 중심으로 -)

  • Kang, Sang-Hun;Kim, Dae Young
    • Korean Journal of Construction Engineering and Management
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    • v.20 no.2
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    • pp.60-67
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    • 2019
  • The main purpose of this study is to identify the tasks performed at the construction stage of apartment houses and analyze the major construction tasks that may affect the performance indicators and analyze the impact on project performance. Based on expert opinions through preliminary research, pilot data, construction database, and other research trends, domestic and foreign research trends are divided into five major tasks, and each is divided into process management (P'1 ~ P'6), construction management (C'1 ~ C'5), environmental management (E'1 ~ E'4), quality control (Q'1 ~ Q'5), safety management (S'1 ~ S'5). Subsequently, each major task was subdivided into 24 detailed tasks. An independent t-test was conducted to analyze the impact on cost. We compared the importance of achieving the project goal and identified the construction work of concrete important construction which affects not only the cost but also the schedule performance index for the degree of significance of less than 0.05.

THE PREVENTIVE EFFECT OF COMMERCIALLY AVAILABLE ANTICARIOGENIC PRODUCTS ON ENAMEL EROSION INDUCED BY CARBONATED BEVERAGE IN VITRO (탄산음료 유발 법랑질 침식에 대한 항우식 제품의 예방 효과)

  • Song, Ju-Hyun;Kim, Young-Jae;Kim, Jung-Wook;Jang, Ki-Taeg;Kim, Chong-Chul;Hahn, Se-Hyun;Lee, Sang-Hoon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.1
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    • pp.1-12
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    • 2010
  • The aim of this study was to evaluate the preventive effect of commercially available anticariogenic products, specifically, the tooth cream containing Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP), fluoride varnish and low-level fluoride mouthrinse on enamel erosion induced by carbonated beverage in a short period of time. Enamel specimens were treated as follows and were then kept in artificial saliva for 24 hours followed by further processing by alternately soaking them in Cola beverage and in distilled water for 1 minute each five times. Group 1: control group (no treatment) Group 2: tooth cream with CPP-ACP Group 3: fluoride varnish (1,000 ppm F) Group 4: low-level fluoride mouthrinse (227 ppm F) Group 5: fluoride varnish + tooth cream with CPP-ACP Group 6: low-level fluoride mouthrinse + tooth cream with CPP-ACP Microhardness and erosion depth were measured and the mineral loss of each specimen was evaluated by measuring the volumetric fluorescence change(${\Delta}Q$) against the stable fluorescent grid using quantitative light-induced fluorescence(QLF). The experiment lasted for 6 days repeated each day. The results were as follows: 1. The microhardness was increased as follows: Group $1{\leq}2{\leq}4$<6<$3{\fallingdotseq}5$. 2. The mean erosion depth was increased as follows: Group $5{\fallingdotseq}3$<6<$4{\fallingdotseq}2{\fallingdotseq}1$. 3. The ${\Delta}Q$ was increased as follows: Group $1{\fallingdotseq}2{\leq}4{\leq}6{\leq}3{\fallingdotseq}5$. The decrement of ${\Delta}Q$ was similar between group 1 and 2, group 4 and 6 and group 3 and 5. 4. The ${\Delta}Q$ showed positive correlation with microhardness (r=0.96, p<0.05), while it was negatively correlated to erosion depth (r=-0.96, p<0.05).

CComparative evaluation of the methods of producing planar image results by using Q-Metrix method of SPECT/CT in Lung Perfusion Scan (Lung Perfusion scan에서 SPECT-CT의 Q-Metrix방법과 평면영상 결과 산출방법에 대한 비교평가)

  • Ha, Tae Hwan;Lim, Jung Jin;Do, Yong Ho;Cho, Sung Wook;Noh, Gyeong Woon
    • The Korean Journal of Nuclear Medicine Technology
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    • v.22 no.1
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    • pp.90-97
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    • 2018
  • Purpose The lung segment ratio which is obtained through quantitative analyses of lung perfusion scan images is calculated to evaluate the lung function pre and post surgery. In this Study, the planar image production methods by using Q-Metrix (GE Healthcare, USA) program capable of not only quantitative analysis but also computation of the segment ratio after having performed SPECT/CT are comparatively evaluated. Materials and Methods Lung perfusion scan and SPECT/CT were performed on 50 lung cancer patients prior to surgery who visited our hospital from May 1, 2015 to September 13, 2016 by using Discovery 670(GE Healthcare, USA) equipment. AP(Anterior Posterior)method that uses planar image divided the frontal and rear images into three rectangular portions by means of ROI tool while PO(Posterior Oblique)method computed the segment ratio by dividing the right lobe into three parts and the left lobe into two parts on the oblique image. Segment ratio was computed by setting the ROI and VOI in the CT image by using Q-Metrix program and statistically analysis was performed with SPSS Ver. 23. Results Regarding the correlation concordance rate of Q-Metrix and AP methods, RUL(Right upper lobe), RML(Right middle lobe) and RLL(Right lower lobe) were 0.224, 0.035 and 0.447. LUL(Left upper lobe) and LLL(Left lower lobe) were found to be 0.643 and 0.456, respectively. In the PO method, the right lobe were 0.663, 0.623 and 0.702, respectively, while the left lobe were 0.754 and 0.823. When comparison was made by using the Paired sample T-test, Right lobe were $11.6{\pm}4.5$, $26.9{\pm}6.2$ and $17.8{\pm}4.2$, respectively in the AP method. Left lobe were $28.4{\pm}4.8$ and $15.4{\pm}5.6$. The right lobe of PO had values of $17.4{\pm}5.0$, $10.5{\pm}3.6$ and $27.3{\pm}6.0$, while the left lobe had values of $21.6{\pm}4.8$ and $23.1{\pm}6.6$, thereby having statistically significant difference in comparison to the Q-Metrix method for each of the lobes (P<0.05). However, there was no statistically significant difference in Right middle lobe (P>0.05). Conclusion The AP method showed low concordance rate in correlation with the Q-Metrix method. However, PO method displayed high concordance rate overall. although AP method had significant differences in all lobes, there was no significant difference in Right middle lobe of PO method. Therefore, at the time of production of lung perfusion scan results, utilization of Q-Metrix method of SPECT/CT would be useful in computation of accurate resultant values. Moreover, it is deemed possible to expect obtain more practical sectional computation result values by using PO method at the time of planar image acquisition.

Distribution of Telomeric DNA in Korean Native Chicken Chromosomes (한국 재래닭 염색체의 텔로미어 분포 양상)

  • Sohn, Sea-Hwan;Cho, Eun-Jeong
    • Korean Journal of Poultry Science
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    • v.37 no.3
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    • pp.247-253
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    • 2010
  • Telomeres are nucleoprotein structures at the ends of chromosomes consisting of DNA sequences arranged in tandemly repeated units $(TTAGGG)_n$. However, this hexamers can also occur at non-telomeric sites in some avians and vertbrate. This study was carried out to present the distribution of telomeric DNA sequences in Korean Native Chicken chromosomes. The fluorescence in situ hybridization technique using a telomeric DNA probe was performed to the metaphase spreads of chicken early embryonic cells. Telomeric DNA signals were detected at the ends of chromosomes including macrochromosomes and microchromosomes. In chicken, surprisingly, chromosome 1 showed very distinct interstitial telomeric DNA hybridization patterns which located two interstitial sites in the p-arm at 1p11 and 1p23, and one in the q-arm at 1q32. In chromosome number 2 and 3 also displayed interstitial telomeric signals (ITS) in the long arms at 2q24 and 3q32, respectively. The pattern of telomeric DNA distribution in Korean Native Chicken chromosomes was in agreement with a previously reported in Gallus domesticus. The relative amount of telomeric DNA sequences in each macrochromosomes ranged from 4.6% to 16.3%. Distribution of telomeric DNAs at the end of p-arm was much more than that of q-arm in almost chicken chromosomes. The distribution of ITS in chicken chromsomes implicate to tandem chromosome fusions that might have occurred during the process of karyotype evolution.

Effects of Head-down Tilt $(-6^{\circ})$ on Peripheral Blood Flow in Dogs (두부하위$(-6^{\circ})$로의 체위변동이 말초혈류에 미치는 영향)

  • Chae, E-Up;Yang, Seon-Young;Bae, Jae-Hoon;Song, Dae-Kyu
    • The Korean Journal of Physiology
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    • v.24 no.1
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    • pp.51-65
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    • 1990
  • The purpose of the present study was to examine the hemodynamic responses, especially in arterial and skin blood flows, in conjunction with the changes of plasma catecholamine levels as an indirect marker of adrenergic tone during the early stage of head-down tilt (HDT), and to evaluate the early physiological regulatory mechanism in simulated weightlessness. Ten mongrel dogs, weighing8\;{\sim}\;14\;kg, were intravenously anesthetized with nembutal, and postural changes were performed by using the tilting table. The postural changes were performed in the following order: supine, prone, HDT $(-6^{\circ}C)$ and lastly recovery prone position. The duration of each position was 30 minutes. The measurements were made before, during and after each postural change. The arterial blood flow $({\.{Q}})$ at the left common carotid and right brachial arteries was measured by the electromagnetic flowmeter. Blood pressure (BP) was directly measured by pressure transducer in the left brachial artery. To evaluate the peripheral blood flow, skin blood flow $({\.{Q}})$ was calculated by the percent changes of photoelectric pulse amplitude on the forepaw, and skin temperature was recorded. The peripheral vascular resistance (PR) was calculated by dividing respective mean BP values by ${\.{Q}}$ of both sides of common carotid and brachial arteries. Heart rate (HR), respiratory rate (f) and PH, $Po_{2},\;Pco_{2}$ and hematocrit of arterial and venous blood were also measured. The concentration of plasma epinephrine and norepinephrine was measured by radioenzymatic method. The results are summarized as follows: Tilting to head-down position from prone position, HR was initially increased (p<0.05) and BP was not significantly changed. While ${\.{Q}}$ of the common carotid artery was decreased (p<0.05) and PR through the head was increased, ${\.{Q}}$ of the brachial artery was increased (p<0.05) and PR through forelimbs was decreased. ${\.{Q}}$ of the forepaw was initially increased (p<0.05) and then slightly decreased, on the whole revealing an increasing trend. Plasma norepinephrine was slightly decreased and the epinephrine was slightly increased. f was increased and arterial pH was increased (p<0.05). In conclusion, the central blood pooling during HDT shows an increased HR via Bainbridge reflex and an increased ${\.{Q}}$ of the forepaw and brachial ${\.{Q}}$, due to decreased PR which may be originated from the depressor reflex of cardiopulmonary baroreceptors. It is suggested that the blood flow to the brain was adequately regulated throughout HDT $(-6^{\circ}C)$ in spite of central blood pooling. And it is apparent that the changes of plasma norepinephrine level are inversely proportional to those of ${\.{Q}}$ of the forepaw, and the changes of epinephrine level are paralleled with those of the brachial ${\.{Q}}$.

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Identification of a Potential Gene for Elevation ω-3 Concentration and its Efficiency for Improving ω-6/ω-3 Ratio in Soybean

  • Hyun Jo;Jeong-Dong Lee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.24-24
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    • 2022
  • This present study was to identify a novel candidate gene that contribute to the elevated α-linolenic acid (ALA, ω-3) concentration in PE2166 from mutagenesis of Pungsannamul. Major loci qALA5_1 and qALA5_2 were detected on chromosome 5 of soybean through quantitative trait loci mapping analyses of recombinant inbred lines. With next generation sequencing of parental lines and Pungsannamul, and recombinant analyses, a potential gene, Glyma. 05g221500 (HD) controlling elevated ALA concentration was identified. HD is a homeodomain-like transcriptional regulator that may regulate the expression level of microsomal ω-3 fatty acid desaturase (FAD3) genes responsible for the conversion of linoleic acid into ALA in the fatty acid biosynthetic pathway. In addition, we hypothesized that combination of mutant alleles, HD and either of microsomal delta-12 fatty acid desaturase 2-1 (FAD2-1\ could reduce the ω-6/ω-3 ratio. In populations where HD, and FAD2-1A and FAD2-1B genes were segregated, combination of a hd allele from PE2166 and either of the variant FAD2-1 alleles were sufficient to reduce the ω-6/ω-3 ratio in seeds.

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GENERALIZATION OF THE SCHENSTED ALGORITHM FOR RIM HOOK TABLEAUX

  • Lee, Jaejin
    • Korean Journal of Mathematics
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    • v.24 no.3
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    • pp.469-487
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    • 2016
  • In [6] Schensted constructed the Schensted algorithm, which gives a bijection between permutations and pairs of standard tableaux of the same shape. Stanton and White [8] gave analog of the Schensted algorithm for rim hook tableaux. In this paper we give a generalization of Stanton and White's Schensted algorithm for rim hook tableaux. If k is a fixed positive integer, it shows a one-to-one correspondence between all generalized hook permutations $\mathcal{H}$ of size k and all pairs (P, Q), where P and Q are semistandard k-rim hook tableaux and k-rim hook tableaux of the same shape, respectively.

Observation on the Low Back Pain with Myofascial Pain Syndrome (근막동통 증후군의 요통환자에 대한 고찰)

  • Her, Young-Gu
    • Journal of Korean Physical Therapy Science
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    • v.1 no.2
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    • pp.235-241
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    • 1994
  • Myofascial pain syndrome is a common cause of physical disability and reduces the activity of the patient. The purpose of this study was review and analysis efficiency of myofascial pain syndrome with low back pain on 50 case who were treated at the Tae Jon Nam, Myung Ho rehabilitation clinic, from January 1, 1994 to May 31, 1994. The results of this study are as follows : 1. of the 50 cases, 24(48.0%) were male and 26(52.0 %) were female. The most common age group was 40 to 30 years old. 2. The most common duration of the treatment and onset were more than 25 months with 22(44.0 %) and less : 3. As for a major causative disease of low back pain with myofascial pain sndrome, without known cause(30.0 %), lifting object(16.0 %), post exercise(14.0 %) and heavy work(12.0 %). 4. Involved muscle with low back pain reviewed I. C. L(31.0 %), Q. L, Gluteus and others muscle(23.0 %). 5. Disease history and treatment duration were proportioned. 6. Involved muscle distribution for causative disease was 26.0 % post exercise to I. C. L, 33.5 % lifting object to Q. L, 40.0 % without known to gluteus. 7. Low back pain with syndrome was buttock and thigh Pain with 44.0 %, only low back pain with 36.0 %. 8. The improvement by physical therapy on the low back pain was good 24.0 %, fair 56.0 % etc respectively. * I.C.L : Iliocostalis lumborum * Q.L : Quadratus lumborum.

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