• The Korean Journal of Mycology
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• v.19 no.1
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• pp.61-65
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• 1991

For the production of mycelia of mushroom by submerged culture, the experiment was carried out. mushroom. The optimum culture broth for Ganoderma lucidum NG-L were compo­sed of CMC 0.8%(w/v) and $(NH_4)_2SO_4$ 0.2%, with 10%(v/v) of inoculum size and pH 5.5 when the milk whey was used as basal medium. In case of Grifola frondosa ATCC48688, the optimum broth were composed of soluble starch 2%(w/v) and $KNO_3$ 0.l%(w/v), with 8%(v/v) of inoculum size and pH 5.2. Among several plant growth hormones,indole-3-acetic acid and gibberellin $A_3-3-acetate$ stimulated the mycelial growth of Ganoderma lucidum NG-L and Grifola frondosa ATCC 48688 respectively. The culture broth of these mushrooms inhibited the growth of B. subtilis and P. aeruginosa.

• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.358-365
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• 2015

ε-Poly-L-lysine (ε-PL) is a homopolymer of L-lysine molecules connected between the epsilon amino and alpha carboxyl groups. This polymer is currently used as a natural preservative in food. Insufficient biomass is a major problem in ε-PL fermentation. Here, to improve cell growth and ε-PL productivity, various nitrogen-rich nutrients were supplemented into flask cultures after 16 h cultivation, marking the onset of ε-PL biosynthesis. Yeast extract, soybean powder, corn powder, and beef extract significantly improved cell growth. In terms of ε-PL productivity, yeast extract at 0.5% (w/v) gave the maximum yield (2.24 g/l), 115.4% higher than the control (1.04 g/l), followed by soybean powder (1.86 g/l) at 1% (w/v) and corn powder (1.72 g/l) at 1% (w/v). However, supplementation with beef extract inhibited ε-PL production. The optimal time for supplementation for all nutrients examined was at 16 h cultivation. The kinetics of yeast-extract-supplemented cultures showed enhanced cell growth and production duration. Thus, the most commonly used two-stage pH control fed-batch fermentation method was modified by omitting the pH 5.0-controlled period, and coupling the procedure with nutrient feeding in the pH 3.9-controlled phase. Using this process, by continuously feeding 0.5 g/h of yeast extract, soybean powder, or corn powder into cultures in a 30 L fermenter, the final ε-PL titer reached 28.2 g/l, 23.7 g/l, and 21.4 g/l, respectively, 91.8%, 61.2%, and 45.6% higher than that of the control (14.7 g/l). This describes a promising option for the mass production of ε-PL.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.3
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• pp.165-170
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• 1991

Effects of liming and starch application on the soil pH and mineralization of organic nitrogen were compared to control at $30+/10^{\circ}C$ incubation for six weeks in two soil types(loam, sandy loam) adjusted with 80% of field moisture capacity. 1. While soil pH was increased abruptyl one week after incubation in loam applied with lime and lime+starch, it tended to decrease at control during incubation period. Liming on sandy loam increased soil pH, and application of lime+starch together showed high pH at first week of incubation which was lowered slowly after wards. Significant changes in soil pH was not detected at control or starch application only. 2. The content of $NH_3-N$ in loam started to increse three weeks after incubation, and was maximized at fifth week. Positive correlation was shown at control during incubation period between soil pH and $NO_3-N$ content. Level of $NO_3-N$ content applied with starch or lime+starch was low compared to control. 3. The $NH_4-N$ content was high when sandy loam was applied with lime+starch together. At the first week of incubation, the $NO_3-N$ content was high at control or lime application.

• 한국초전도학회:학술대회논문집
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• 2007.07a
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• pp.6-6
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• 2007

• Food Science and Preservation
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• v.23 no.7
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• pp.1042-1049
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• 2016

This study was conducted to investigate and compare the characteristics of acetic acid fermentation in detoxified Rhus verniciflua vinegar (DRV) produced by different yeast strains. The DRVs were prepared by static acetic acid fermentation using six different yeast strains (Saccharomyces cerevisiae Fermivin, Pichia kudriavzerii N77-4, Hanseniaspora pountiae HP1-2, Candida tropicalis Y447, Wickerhamomyces anomalus N43-8, and Pichia kluyveri Frootzen). Alcohol content of the S. cerevisiae Fermivin fermented DRV was highest 16.07%. Among the yeast strain DRVs, there were significant differences in alcohol content, but all alcohol levels were 11%. Moreover, there were differences in pH and titratable acidity of the DRVs. The organic acid content of the DRVs ranged from 35.88 to 55.49 mg/mL and there were significant differences among the yeast strain DRVs. Essential free amino acids, particularly glutamic acid, alanine, leucine and valine, were detected in each of the 6 DRVs. Electronic nose analysis revealed that three different volatile chemical patterns were present in the 6 DRVs. The results indicate that yeast strains with different characteristics can produce vinegars with different characteristics.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.223-229
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• 2002

For the research of the natural marine antioxidant, several bacteria were isolated from the coast of jin-Hae in Korea. Among the marine bacteria studied, strain HJ-14, a gram-negative, motile, strait rod, aerobic, and $Na^{+}$ required bacterium showed high activity of 1,1-diphenyl-2-picrylhydrazyl radical scav- enging. The morphological, physiological, and biochemical characteristics of the strain HJ-14 were similar to those of the Alteromonas macleodii ATCC $27126^{T}$ . Thus, it was tentatively identified as Alteromonas sp. HJ-14. The compositions of major fatty acids in cell membrane of Alteromonas sp. HJ-14 were $C_{ 14:0}$ , $ C_{15:0}$ , $C_{16:0}$ and $C_{17:1}$ $_{w8c}$ , which also suggest that it is affiliated with Alteromonas sp. The optimum culture conditions for production of antioxidant materials with Alteromonas sp. HJ-14 were at $25$~$37^{\circ}C$ and pH 6~8. The optimum conditions for the production of antioxidant for carbon, inorganic nitrogen, and sodium chloride sources were 2.5%(w/v) dextrin, 0.5%(w/v) ammonium sulfate, and 2~6%(w/v) sodium chloride, respectively. The hydroxyl radical scavenging ability of Alteromonas sp. HJ-14 broth was 90.03%, which is higher than ascor-bic acid(83.28%) and lower than butylated hydroxyanisole(95.46%) and $\alpha$-tocopherol(97.17%).

• Journal of the Korean Wood Science and Technology
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• v.47 no.4
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• pp.393-407
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• 2019

In this study, we prepared lignocellulosic artificial soil that contains Bacillus subtilis (peat moss/perlite/ steam-exploded oak wood/microbial culture = 3:1:3:3, w/w/w/w) for use in the restoration of damaged soil areas. The prepared lignocellulosic artificial soil was mixed with soil at ratios of 0%, 25%, 50%, 75%, and 100%. These mixed soils were then applied to fields, and the resultant physicochemical properties and their effects on the plant growth of Lespedeza cyrtobotrya were observed. The mixture of the prepared artificial soils (mixed at ratios of 25%-100%) with soil had a bulk densities of <$0.04g/cm^3$, porosities of >85%, pH values between 4.3 and 4.7, electrical conductivities of <0.5 dS/m, C/N ratios between 15.0 and 26.5, organic matter content between 23.6% and 43.2%, and bacterial densities between $157{\times}10^6$ and $624{\times}10^6CFU/g$. In addition, the prepared artificial soils mixed with soil at ratios of 25%-50% exhibited higher plant growth rates for L. cyrtobotrya compared with the control. Overall, we identified positive correlations between the plant growth of L. cyrtobotrya and soil bulk density, porosity, water-holding capacity, C/N ratio, organic matter, and bacterial densities.

• Journal of Food Hygiene and Safety
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• v.38 no.6
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• pp.449-456
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• 2023

In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 10⁰ CFU/mL to 10⁶ CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)-mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37℃. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 10⁶ CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.299-309
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• 1997

This experiment was conducted to determine the mineral absorption character and develop the optimum composition of nutrient solution for dill(Anethum graveolens L.) in hydroponics. Dill(Anethum graveolens L.) plants were grown in nutrient film technique(NFT) supplied with 1/4, 1/2, and 1 strength of the nutrient solution developed by National Research Station in Japan(HRS). Plants grown in 1/2 strength showed the best growth in plant height, fresh weight, and dry weight compared with those grown in 1/4 or 1 strength. In 1/2 strength solution, pH and EC changed little and proper nutrient contents were observed in the leaves as compared to plant nutrient diagnosis standard. Based on these results, optimum macronutrients were composed by nutrient- water absorption rate(n/w) with 1/2 strength: NO$_2$―N 8.85, NH$_4$―N 0.55, P 2.1, K 6.2, Ca 2.8, and Mg 1.7 me L$^{-1}$ To examine the suitability of the nutrient solution developed(SCU) , dill plants were grown in NFT supplied with two different kinds of solution and concentration. 1/2, 1, 3/2 and 25 of SCU and 1/2S of HRS. Changes of pH and EC were not distinct in 1S, but a significant change of pH was shown in low concentrations-HRS 1/2S and SCU 1/2S. Shoot fresh and dry weight were much higher in the plants grown in SCU IS as compared with HRS 1/2S. There were no significant differences in growth of plants grown in SCU IS, 3/2S, and 25. In addition, nutrient contents in the leaves grown by SCU 1S were in proper levels as compared with plant nutrient diagnosis standard. SCU 1S developed in this experiment was found to be optimum for dill in hydroponics.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.127-133
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• 1999

The purpose of this study was to determine the optimum hydrolysis conditions for the production of enzymatic hydrolysate from tuna boiled extract (TBE) using membrane (molecular weight cut off 10,000Da) reator. The tuna pyloric caeca crude enzyme (TPCCE) was identified as the most suitable enzymes for the hydrolysis of TBE. The optimum hydrolysis conditions of TBE in the batch reactor were $40^{\circ}C$, pH 9 and substrate to TPCCE ratio 50 (w/w). For 6hr under the above conditions, $70\%$ of the total amount of initial TBE was hydrolysed. The optimum hydrolysis conditions of TBE in the membrane reactor were $40^{\circ}C$, pH 9, enzyme 0,1 g/$\ell$, volume 1$\ell$ and substrate to enzyme ratio 100(w/w). The degree of hydrolysis of TBE was above $60\%$ for 3 hr. The TBE hydrolysate were prepared with $5\%$ TBE solution under the optimum hydrolytic conditions in the membrane reactor