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Isolation of Phytase-Producing Pseudomonas sp. and Optimization of its Phytase Production

  • Kim, Young-Hoon;Gwon, Moon-Nam;Yang, Si-Yong;Park, Tae-Kyu;Kim, Chan-Gil;Kim, Chang-Won;Song, Min-Dong
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.279-285
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    • 2002
  • Phytase (myo-inositol hexakisphosphate phospho-hydrolase, EC 3.1.3.8) catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to release inorganic phosphate. A bacterial strain producing phytase was isolated from soil around a cattle shed. To identify the strain, cellular fatty acids profiles, the GC contents, a quinine-type analysis, and physiological test using an API 20NE kit were carried out. The strain was identified to be a genus of Pseudomonas sp. and named as Pseudomonas sp. YH40. The optimum culture condition for the maximum productivity of phytase by Pseudomonas sp. YH40 were attained in a culture medium composed of $1.0\%$ (w/v) glycerol, $2.0\%$ (w/v) peptone, and $0.2\%$ (w/v) $FeSO_4{\cdot}7H_2O$. Within the optimal medium condition, the production of phytase became highest after 10 h of incubation, and the maximal phytase production by Pseudomonas sp. YH40 was observed at $37^{\circ}C$ and pH 6.0.

Optimization of CANFLEX-RU Fuel Bundle for CANDU-6

  • Lee, Y. O.;C. J. Jeong;K. S. Sim;J. S. Jun;Park, G. S.;Kim, B. G.;Park, J. H.;H. C. Suk
    • Proceedings of the Korean Nuclear Society Conference
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    • 1995.10a
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    • pp.35-40
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    • 1995
  • Considering the higher discharge burnup, lower channel refuelling rate, lower linear element rating(LER), lower coolant void reactivity and axial power shape, CANFLEX-RU fuel bundle is optimized for CANDU-6 by grading the fissile composition in the ring-wise of the bundle and by applying fuel management scheme appropriately. The fissile composition of the fuel bundle is graded as the recovered uranium (0.9 w/o U-235) in the outer and intermediate elements, depleted Uranium (0.2 w/o U-235) in the center element, natural uranium (0.71 w/o U-235) in the inner elements. Enrichment is not required for these fuel. The fissile composition is optimized by lattice calculation and by time-averaged reactor simulation. CANFLEX-RU optimized for CANDU-6 resulted to be the 15% lower channel refuelling rate, acceptable axial power profile and power envelope, 70% higher discharge burnup, 15% lower LER and not increase coolant void reactivity compared with the 37-element natural uranium bundle for CANDU-6.

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Studies on Separation and Determination of Korean Bovine Serum Protein by Colorimetric Method (비색법에 의한 한우 혈청단백질의 분획정량 시험)

  • Cho, J.H.
    • Korean Journal of Veterinary Research
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    • v.11 no.2
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    • pp.145-148
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    • 1971
  • Serum Samples from adult of Korean cattles including 40 females and 20 males were analyzed by sodium salt precipitation and colorimetric method in the purpose of the determination of total serum protein, albumin, globulin, ${\alpha}$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin. The results obtained arc summarized as follows: 1. Mean value of total serum protein showed a slight variation from 7.6%, and its regional and sex differences were not found to be significant. 2. Contents of albumin in serum showed lower level than that of globulin as low level of A/G ratio 0.4 in proportion. 3. Contents of Serum ${\alpha}$-globulin showed 1.4w/v% and $1.51{\pm}0.46$w/v% in each group of female, and $1.31{\pm}0.26$w/v%, in the group of male. 4. Contents of serum ${\beta}$-globulin showed 1.74w/v%, 1.95w/v%, in each group of female, and 1.82w/v% in the group of male. 5. Contents of serum ${\gamma}$-globulin showed 2.32w/v%, 2.30w/v% in each group of female, and 2.30w/v%, in the group of male.

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Removal of toxic hydroquinone: Comparative studies on use of iron impregnated granular activated carbon as an adsorbent and catalyst

  • Tyagi, Ankit;Das, Susmita;Srivastava, Vimal Chandra
    • Environmental Engineering Research
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    • v.24 no.3
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    • pp.474-483
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    • 2019
  • In this study, iron (Fe) impregnated granular activated carbon (Fe-GAC) has been synthesized and characterized for various properties. Comparative studies have been performed for use of Fe-GAC as an adsorbent as well as a catalyst during catalytic oxidation of hydroquinone (HQ). In the batch adsorption study, effect of process parameter like initial HQ concentration ($C_o=25-1,000mg/L$), pH (2-10), contact time (t: 0-24 h), temperature (T: $15-45^{\circ}C$) and adsorbent dose (w: 5-50 g/L) have been studied. Maximum HQ adsorption efficiency of 75% was obtained at optimum parametric condition of: pH = 4, w = 40 g/L and t = 14 h. Pseudo-second order model best-fitted the HQ adsorption kinetics whereas Langmuir model best-represented the isothermal equilibrium behavior. During oxidation studies, effect of various process parameters like initial HQ concentration ($C_o:20-100mg/L$), pH (4-8), oxidant dose ($C_{H2O2}:0.4-1.6mL/L$) and catalyst dose (m: 0.5-1.5 g/L) have been optimized using Taguchi experimental design matrix. Maximum HQ removal efficiency of 83.56% was obtained at optimum condition of $C_o=100mg/L$, pH = 6, $C_{H2O2}=0.4mL/L,$ and m = 1 g/L. Overall use of Fe-GAC during catalytic oxidation seems to be a better as compared to its use an adsorbent for treatment of HQ bearing wastewater.

Effects of $N_2O$/$SiH_4$Flow Ratio and RF Power on Properties of $SiO_2$Thick Films Deposited by Plasma Enhanced Chemical Vapor Deposition (PECVD법에 의해 증착된 $SiO_2$후막 특성에서 $N_2O$/$SiH_4$Flow Ratio와 RF Power가 미치는 영향)

  • 조성민;김용탁;서용곤;임영민;윤대호
    • Journal of the Korean Ceramic Society
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    • v.38 no.11
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    • pp.1037-1041
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    • 2001
  • Silicon diosixde thick film using silica optical waveguide cladding was fabricated by Plasma Enhanced Chemical Vapor Deposition (PECVD) method, at a low temperature (32$0^{\circ}C$) and from (SiH$_4$+$N_2$O) gas mixtures. The effects of deposition parameters on properties of SiO$_2$thick films were investigated by variation of $N_2$O/SiH$_4$flow ratio and RF power. As the $N_2$O/SiH$_4$flow ratio decreased, deposition rate increased from 2.9${\mu}{\textrm}{m}$/h to maximum 10.1${\mu}{\textrm}{m}$/h. As the RF power increased from 60 W to 120 W, deposition rate increased (5.2~6.7 ${\mu}{\textrm}{m}$/h) and refractive index approached at thermally grown silicon dioxide (n=1.46).

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Color-stabilized organic light-emitting devices by using N, N'-bis-(1- naphthyl)-N, N'-diphenyl-1,1-biphenyl-4,4'-diamine/5,6,11,12 - tetraphenylnaphthacene multiple quantum well structures

  • Yoon, Y.B.;Kim, T.W.;Yang, H.W.;Lee, H.G.;Kim, J.H.;Kim, Y.G.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07b
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    • pp.1378-1380
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    • 2005
  • The efficiency and the optical properties of the yellow organic light-emitting devices (OLEDs) were significantly affected by the existence of the multiple quantum well (MQW) structures consisting of N, N'- bis-(1-naphthyl)-N, N'-diphenyl-1,1-biphenyl-4,4'- diamine(NPB)/5,6,11,12 - tetraphenylnaphthacene (rubrene). The maximum efficiency and the luminance of OLEDs with 3-periods of the NPB/rubrene MQWs at 41.6 $mA/cm^2$ were 3.66 cd/A and 1524 $cd/m^2$, respectively, and their Commission Internationale de l'Eclairage chromaticity coordinates were (0.34, 0.55), which indicates a yellow color. These results indicate that the efficiencies of the OLEDs by using MQW emitting layers can be improved.

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Status Summary of Local Controller for the ITER 전원장치 (ITER 전원장치 Local Controller Design Status Summary)

  • Suh, J.H.;Yoo, M.H.;Oh, J.S.;Choi, J.W.;Choi, J.H.;Shin, H.K;Park, H.J.;Lee, L.S.;Kim, C.W.
    • Proceedings of the KIPE Conference
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    • 2016.11a
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    • pp.188-189
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    • 2016
  • ITER AC/DC Converter Local Controller는 플라즈마 제어 시스템에서 요구하는 전압 전류 명령에 따라 초전도 코일에 전류를 공급하고 컨버터 System 자체 보호 기능, 초전도 코일시스템 이상 시 Bypass 및 코일 에너지를 계통에 회생하여 에너지를 방전하여야 한다. 코일 별로 플라즈마 제어에 필요한 높은 전압 전류는 전원 장치의 직렬 접속이 요구되고(2직렬, 4직렬, 6직렬) 제어적으로 이들 제어 시스템들은 신뢰도가 높은 디지털 설계를 요구 한다. 본 논문은 다 직렬 전원 장치 제어를 위한 Local Controller의 설계 내용을 논의하고자 한다.

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CO2 Respiration Characteristics with Physicochemical Properties of Soils at the Coastal Ecosystem in Suncheon Bay (순천만 연안 생태계에서 토양의 이화학적 성질에 의한 이산화탄소 호흡 특성)

  • Kang, Dong-Hwan;Kwon, Byung-Hyuk;Kim, Pil-Geun
    • Journal of Environmental Science International
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    • v.19 no.2
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    • pp.217-227
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    • 2010
  • This paper was studied $CO_2$ respiration rate with physicochemical properties of soils at wetland, paddy field and forest in Nongju-ri, Haeryong-myeon, Suncheon city, Jeollanam-do. Soil temperature and $CO_2$ respiration rate were measured at the field, and soil pH, moisture and soil organic carbon were analyzed in laboratory. Field monitoring was conducted at 6 points (W3, W7, W13, W17, W23, W27) for wetland, 3 points (P1, P2, P3) for paddy field and 3 points (F1, F2, F3) for forest in 10 January 2009. $CO_2$ concentrations in chamber were measured 352~382 ppm for wetland, 364~382 ppm for paddy field and 379~390 ppm for forest, and the average values were 370 ppm, 370 ppm and 385 ppm, respectively. $CO_2$ respiration rates of soils were measured $-73{\sim}44\;mg/m^2/hr$ for wetland, $-74{\sim}24\;mg/m^2/hr$ for paddy field and $-55{\sim}106\;mg/m^2/hr$ for forest, and the average values were $-8\;mg/m^2/hr$, $-25\;mg/m^2/hr$ and $38\;mg/m^2/hr$. $CO_2$ was uptake from air to soil in wetland and paddy field, but it was emission from soil to air in forest. $CO_2$ respiration rate function in uptake condition increased exponential and linear as soil temperature and soil organic carbon. But, it in emission condition decreased linear as soil temperature and soil organic carbon. $CO_2$ respiration rate function in wetland decreased linear as soil moisture, but its in paddy and forest increased linear as soil moisture. $CO_2$ respiration rate function in all sites increased linear as soil pH, and increasing rate at forest was highest.

Sonolysis of Trichloroethylene in a Continuous Flow Reactor with the Multi Ultrasound Irradiation (연속식 다중 초음파 반응조에서 TCE의 초음파 분해)

  • Lee, Min-Ju;Oh, Je-Ill
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.6
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    • pp.419-427
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    • 2009
  • To test applicability for continuous flow treatment of ultrasound technology, sonolysis of TCE aqueous solution using 584 kHz multi irradiation reactor was performed under batch and continuous flow conditions. Under batch condition (3 and 4 sides irradiation, 600 W), first order degradation rate constant of TCE was higher under 4 sides than 3 sides irradiation conditions, while the generation of $H_2O_2$ and chloride was similar under both irradiation conditions. Under continuous flow condition with 4 sides irradiation, removal efficiencies of TCE in steady-state were decreased from 83 to 48% with increasing flow rate from 67 to 300 mL/min at 600 W, and were increased from 14 to 75% with increasing acoustic power from 100 to 600 W at 100 mL/min. Removal efficiency of TCE in groundwater was decreased 10% compared to in distilled water at 100 mL/min and 600 W.

Preparation and Structure of trans-Bis(tert-butylamine)dichloropalladium(II), trans-[$PdCl_2(t-BuNH_2)_2$] (trans-Bis(tert-butylamine)dichloropalladium(II), trans-[$PdCl_2(t-BuNH_2)_2$의 합성 및 구조)

  • 김혜진;한원석;이순원
    • Korean Journal of Crystallography
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    • v.12 no.1
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    • pp.10-13
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    • 2001
  • Compound PdCl₂(PhCN)₂(1) reacted with tert-butylamine(t-BuNH₂) to give trans-[PdCl₂(t-BuNH₂)₂] (2) Compound 2 was characterized by spectroscopy (¹H-NMR, /sup 13/C{¹H}-NMR, and IR) and X-ray diffraction. Crystallographic data for f2: monoclinic space group p2₁/c, a=6.298(1)Å, b=20.740(2)Å, c=10.731(1)Å, β=92.58(1)°, Z=4, R(wR₂)=0.0207(0.0543).

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