• Journal of Ginseng Research
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• v.5 no.2
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• pp.114-121
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• 1981

Starch was isolated from 4-year-old and 6-year-old ginseng roots and its physical and chemical characteristics were studied. The results obtained were summarized as follows. 1. The shape of ginseng starch granules was polygonal and rounded with its granule size ranging from 2.0 to 7.5$\mu$. The swelling power of 4-year-old ginseng starch was much greater than that of 6-year old ginseng starch. Gelatinization pattern showed that 6-year-old ginseng starch gelatinized rapidly at $65^{\circ}C$, whereas 4-year-old starch continued to gelatinize, without having a definite gelatinization temperature as temperature increased 3. Amylogram of ginseng starch showed that gelatinization initiated at 61$^{\circ}C$ and was completed at 88$^{\circ}C$ with its viscosity reaching at 810 B.U. 4. The amylose contents were 32% and 9% for 4-year-old and 6-year-old ginseng starch, respectively. 5. X-ray diffraction analyses indicated that there were some structural differences between 4-year-old and 6-year-old ginseng starch.

• Food Science and Biotechnology
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• v.16 no.2
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• pp.281-284
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• 2007

The objective of this research is to provide basic information necessary to differentiate between ginseng (Panax ginseng) grown in woods environments and cultivated ginseng. The ginseng saponin (ginsenoside) contents of Korean woods-grown, 4 year-old cultivated, and 6 year-old cultivated ginsengs were determined via HPLC analysis. The total saponins in the woods-grown ginseng (0.648%) were approximately twice that of the 4 year-old cultivated (0.270%) and the 6 year-old cultivated ginsengs (0.280%). The protopanaxadiols (PD)/protopanaxatriols (PT) ratio of the woods-grown ginseng (3.258%) was higher than that of the 4 year-old cultivated (2.456%) and the 6 year-old cultivated ginsengs (2.183%). The $Rb_1/Rg_1$ ratio of the woods-grown ginseng (10.225%) was also higher than those of the 4 year-old cultivated (3.514%) and the 6 year-old cultivated ginsengs (4.865%).

• Journal of Ginseng Research
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• v.6 no.2
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• pp.149-153
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• 1982

To investigate the effect of light intensity on the growth of shoots and roots, water and chlorophyll contents in the ginseng leaf were determined at 5%, l0%, 20%, 30% light transmittance rate (LTR) with 1,2 and 4 year-old ginseng plants in the field. Stem length, size of the leaf, water and chlorophyll contents were decreased as the increase of the light intensity in all ages of ginseng plants. The degree of decrease was severe in the one year-old ginseng compared to that of 2, or 4 year-old ginseng, while there was no difference between the 2 and 4 year-old plants. Root weight per plant was highest at 5% LTR in the one year-old plants, while it was at 20% LTR in the 2 and 4 year-old plants. Generally, demand of light for the growth of one year-old ginseng was lower than those of 2 or 4 year-old plants there was no difference of it among the ginseng plants older than 2 years.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.483-489
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• 2004

Monthly changes of underground growth and ethanol extract content was investigated from April to September in Korean ginseng of 4, 5 and 6 year-old. Taproot's length was repeatedly reduced and recovered without increasing by growth duration, while it's diameter was gradually increased until September of harvest time. Top weight reached to the peak on July, and decreased gradually until harvest time. Root weight of 4, 5 and 6-year-old ginseng was decreased on May, but increased gradually until harvest time except 6-year-old ginseng decreased after July. Relative growth rate (RGR) of root per plant was low on May and August, but high on June, July, and September in 4, 5 and 6 year-old ginseng. RGR of 4 and 5-year-old ginseng in each lateral and fine root showed distinct changes by month, while those showed little seasonal changes in 6 year-old ginseng. Ratio of root above 60g on september was 23%, 60% and 69%, respectively, among 4, 5 and 6 year-old ginseng. Moisture content of root was gradually decreased until september without significant difference from July to september in 4, 5 and 6 year-old ginseng, and its descending order on september was 4, 5 and 6 year-old ginseng. Hardness of taproot was decreased on May, but increased until september in 4 and 5 year-old ginseng, while that of 6 year-old ginseng was gradually decreased from August to september. Extract content of 4, 5 and 6 year-old ginseng in taproot and lateral root was distinctly decreased from March to June, but gradually increased until September. Although extract content of March and May in 4 year-old ginseng on showed higher than that of 5 and 6 year-old ginseng, it of September didn't show significant difference among 4, 5 and 6 year-old ginseng.

• Journal of Ginseng Research
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• v.16 no.1
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• pp.7-12
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• 1992

This study was carried out to investigate the effect of water extracts from 4-, 5- and 6-year old red white ginseng roots on the inhibition of lipolysis in fat cells induced by toxohormone-L which has been known as lipolytic and anorexigenic factors. Toxohormone-L was obtained by partial purification of the ascites fluid from mice which had been inoculated with sarcoma-180. Each water extract of ginseng was effective in vitro against the inhibition of lipolysis induced by the toxohormone-L at the concentrations over 10~100 $\mu\textrm{g}$/ml. At the concentration of 1,000 $\mu\textrm{g}$/ml, the inhibition ratio of lipolysis by the water extracts of 4-, 5- and 6-year old white ginseng roots were 56.3, 59.7 and 59.4%, and those of red ginseng roots were 78.6, 79.1 and 82.5%, respectively, indicating that the 6-year old red ginseng was the most effective in the inhibition of the lipolysis. The total inhibitory activity per gram of ginseng sample was higher in the 4-year and 5-year old white ginseng roots at the concentrations of 100 $\mu\textrm{g}$/ml and 1,000 $\mu\textrm{g}$/ml, respectively, while for the red ginseng it was higher in the 6-year old ginseng than other ages regardless of the reaction concentration. Only once injection of the ginseng extracts(300 mg/ml in saline solution) with 10 ${mu}ell$/g of weight to the mice bearing sarcoma-180 was not effective against the extension of their life spans. On the contrary, the life spans of the mice were rather shortened by the injection of 5-year old white ginseng and 6-year old red ginseng, as compared to that of control.

• Journal of Forest and Environmental Science
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• v.30 no.4
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• pp.405-411
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• 2014

Wild Korean ginseng has been recognized as highly precious medicine since ancient times. Nowadays, the population of wild ginseng in the forest of Korean peninsula is very rare due to indiscreet harvest. In this work, we investigated the plant regeneration via somatic embryogenesis from embryogenic callus of old wild ginseng (more than 50 years-old) and compared the features of plants regenerated from 5-years old and 50 years-old ginseng. Induction of embryogenic callus from adventitious roots of 50 year-old wild ginseng required 83 weeks of culture, but only 10 weeks were sufficient for 5 year-old ginseng. Height and width of plants derived from the old wild ginseng was smaller and slender compared to the plantlets derived from 5 year-old ginseng. Total chlorophyll contents was 2-6 time lower in plantlets regenerated from 50 year-old wild ginseng than those from 5 year-old ginseng, but anthocyanin content was higher in 50 year-old ginseng. Our results revealed that plants regenerated from old wild ginseng have different morphological and physiological characters probably due to age-dependent phenomenon.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.84-91
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• 1989

This study was conducted to Investigate the effects of physico-chemical soil properties on the growth and yield of ginseng. 1 In the field survey, the yields of 6 year old ginseng were 2.46 Kg/3.3$m^2$, 2.13 Kg/3.3$m^2$, 1.44 Kg/3.3 $m^2$ in clay loam, loam and sandy loam soils, respectively 2. The missing plant rate for il year old ginseng were 33.6% and 51.6% in clay loam and sandy loam soils, respectively : the stem length and stem diameter of ginseng plants in sandy loam soil were smaller than those in clay loam soil. 3. Soil aggregation and porosity we're slightly higher in 6 year old ginseng fields than in 2 year ones. 4. Inorganic-N increased in 2 year and 3 year old ginseng fields reaching up to 100-120ppm, however it 1 decreased to 75 ppm, 34 ppm and 25 ppm in 4, 5 and 6 year old ginseng fields, respectively, It varied 1 more greatly in sandy loam soil than in clay loam. 5. The $P_2O_5$, K, Ca, and Mg contents differed little with plant age. Sandy loam had high N and $P_2O_5$ contents but low cation contents. 6. The yield of 6 year old ginseng fields were significantly correlated with clay contents and porosity. The missing Plant rate of 6 year old ginseng had a positive correlations with sand. and N contents.

• Journal of Ginseng Research
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• v.22 no.4
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• pp.294-298
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• 1998

This study was conducted to investigate the effects of growth characteristics and mineral contents of ginseng seedling grown in different locations on ginseng growth and root yield. Ginseng seedling produced in 16 farmers' field at the semi-Yangjik seedbed were planted and cultivated in a field with same cultural practices. Missing plant was increased with increased year of ginseng age, especially severe at 5-year-old ginseng. Rate of missing plant was different among the origins of the seedling, even with the same weights. Negative correlation was noted between the missing rate of 3-year-old ginseng plants and NH4-N content of the seedling, but positive correlation between the missing rate and K2O and Ca contents of the seedling. Root yield of 6-year-old ginseng was also affected by the origins of ginseng seedlings. Root yield was high in ginseng plant from 0.6∼0.9 g see dings compared to those from over 0.9 g seedlings.

• Journal of Ginseng Research
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• v.22 no.2
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• pp.140-146
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• 1998

It was founded that an X-compound is contained in extracts from the root of old red ginseng (6RG) compared with that from the root of 4-year old red ginseng(4RG). Both water extract and petroleum ether extract (PEII) from 6RG or 4RG inhibited the release of [$^{3}H$]-serotonin induced by platelet activating factor (PAF; 40 ng/ml). Water extract and PEll from 6RG Inhibited potently PAF-induced [$^{3}H$]-serotonin release compared with those from 4RG. X-compound out of both water extract and PEll from 6RG inhibited the release of [$^{3}H$]-serotonin inducted by collagen (100 $\mu\textrm{g}$/ml) or thrombin(20 U/ml). X-compound had a synergistic effect with water extract from 4RG on collagen-and thrombin-induced [3H] -serotonin release out of human platelets. The concentration(IC50) of X-compound that require to inhibit 50% of [$^{3}H$]-serotonin-release was 3.25 $\mu\textrm{g}$/ml, and it is inferred that maximum concentration of X-compound that inhibits the release of [$^{3}H$]-serotonin is 10 $\mu\textrm{g}$/ml. Because thrombosis is resulted mainly from the irreversible aggregations which are intimately related with the serotonin release and migraine is also caused when serotonin is released, it is inferred that water extract, PEII and X-compound from 6RG have antithrombosis and antimigrainous functions by inhibiting the release of serotonin from human platelets.

• Journal of Ginseng Research
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• v.14 no.3
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• pp.432-436
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• 1990

Incidence of damping-off callsed by Rhizoctonia solani was 0.6-10.9% at "Yangjik" seedbed in Pocheon, Korea. The seedbeds where the lengths of etiolated stems (underground portion) of ginseng seedlings were 0.78-1.25 cm showed 0.8-3.2% of the disease, while 6.9-10.9% disease incidence was observed at the seedbeds with the longer etiolated stem (1.89-2.26 cm). The pathogen produced a typical girdle symptom on the etiolated portion of ginseng stems close to the soil surface. The deeper the seeds were sown, the more the disease occurred in pot soil inoculated with the pathogen, AG 2-1, showing 18.4, 27.4 and 32.9% of damping-off at the seeding depth of 1, 2 and 4 cm, respectively. Cuticle layers of colored stems (over ground portion) were well - developed to be 42.8, 58.0, and 55.0 um in thickness compared to the etiolated stems with 8.5, 15.0 and 8.0um for seedling, 2 year-old, and 3 year-old ginsengs, respectively, when the disease occurred. In the seedling and 2 year-old ginseng, the colored stems were more rigid than the etiolated. There was however, no difference in rigidness of the stem of the 3 year-old ginseng where the disease is not severe as in seedlings and 2 year-old ginseng plants.ng plants.