• Journal of Fisheries and Marine Sciences Education
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• v.17 no.1
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• pp.132-143
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• 2005

In order to investigate the seasonal variation of watermass and fishery creatures in the adjacent sea of Naro Island, an oceanographic observation was carried out seasonally on the fishing grounds, and the sales performance data of the fisheries cooperative's joint market of Naro Island was examined by using a principal component analysis. The temperature and salinity ranged from 8.1 $^{\circ}C$ to 13.7 $^{\circ}C$ and from 33.1 psu to 34.3 psu in spring, from 14.5 $^{\circ}C$ to 24.2 $^{\circ}C$ and from 30.5 psu to 34.1 psu in summer, from 14.8 $^{\circ}C$ to 18.6 $^{\circ}C$ and from 30.1 psu to 34.0 psu in autumn, and from 4.3 $^{\circ}C$ to 10.1 $^{\circ}C$ and from 33.1 psu to 34.9 psu in winter, respectively. In winter and spring, the offshore water spread out to all sea areas of all water layers. In summer, the mixed waters covered the entire sea surface whereas the mixed water and offshore water covered the bottom. In autumn, the coastal water and mixed water appeared on the surface, but the mixed water was distributed widely on the bottom and the offshore water began to appear in the open sea. For two years from 2002 to 2003, 58 fishery creature species in total were sold in the fisheries cooperative's joint market of Naro Island. In general, the total of 50% fish were sold, and crustacea and mollusc by each 25%. Medium shrimp, whiparm octopus, blue crab, and octopus predominated. A number of species and biomass of fishery creatures were sold mostly in April and May, while they were sold the least in January and February. The seasonal sales results showed that mullet, angler, short necked clam, large shrimp, and webfoot octopus were sold mainly in spring, tonguefish, flathead, pomfret, glass eel, blue crab, whiparm octopus, and squid were sold mainly in summer, and octopus, medium shrimp, and spanish mackerel were sold mainly in autumn.

• ALGAE
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• v.19 no.2
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• pp.145-148
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• 2004

As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.

• Development and Reproduction
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• v.15 no.2
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• pp.151-158
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• 2011

The comparison of the chemical properties of semen of black porgy Acanthopagrus schlegelii long-term acclimated reared in freshwater (BFW) and seawater (BSW) with sperm activity of salinity and ion composition. The chemical properties of seminal plasma on BFW of the factors that most there was not significant difference in the BSW. However, osmolality in seminal plasma of BFW and BSW was $307.0{\pm}4.6$ and $337.3{\pm}10.1$ mOsm/kg, respectively, where BFW showed significant lower concentration in contrast to BSW. Salinity effect on sperm motility of BFW and BSW in 0 psu solution, no sperm motility was observed, whereas in 10 psu solution, both BFW and BSW sperms showed low motility and short time post sperm activation. However, diluted in 20 and 32 psu solutions, highest motility and long time post sperm activation were observed in BFW and BSW sperm. SAI of BFW and BSW varied in depend on the osmolality regardless of ion kind and it showed the highest value in the similar osmolality of artificial seawater (956 mOsm/kg). Accordingly, even in sperm released from BFW, factors initiating sperm motility are determined by osmolality.

• Fisheries and Aquatic Sciences
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• v.9 no.4
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• pp.153-159
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• 2006

The ark shell (or 'blood clam') Scapharca broughtonii is a filter-feeding bivalve that has red blood and inhabits waters approximately 10m in depth off the southern coast of South Korea. This study was part of a larger research project investigating the causes of death and restoration of shellfish resources, which are important aquaculture products in South Korea. We examined physiological responses related to survival, respiration, excretion, and amino acid changes as a result of changes in salinity. The 9-day median lethal salinity ($LS_{50}$) was 16.5 psu with confidence limits of 14.9-18.1 psu. At $25^{\circ}C$, the oxygen consumption and ammonia-nitrogen excretion rates were increased with decreases in salinity. Although the osmolality of individuals was acclimated within 2 h at 26.4 psu and 12 h at 19.8 psu, it took more than 5 days at a salinity of 13.2 psu, whereas no individuals acclimated and all died at a salinity of 6.6 psu. Of the amino acids present in the blood, taurine and alanine increased in response to decreased salinity. Tissues of the gill and the mid-gut gland were affected by decreasing salinity. These data will provide important fundamental information for examining the causes of mass mortality of shellfish in the summer.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.1
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• pp.19-26
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• 2003

In order to investigate the seasonal variation of watermass in the adjacent sea of Naro Island, oceanographic observation on the fishing grounds were carried out by the training vessel of Yosu National University on winter, spring, summer, and autumn in 2000. The results obtained are summarized as follows ; 1) the watermass in the fishing ground were divided into the coastal water (30.0∼31.4psu), mixing water (31.5∼32.9psu) and the offshore water (33.0∼35.0psu) according to the distribution of salinity from T-S diagram plotted all salinity data observed on winter, spring, summer, and autumn in 2000. 2) the ranges of temperature and salinity were from 4.$3\circ_C$ to 10.1$^{\circ}C$ and from 33.1psu to 34.9psu in winter, from 8.$1\circ_C$ to 13.$7\circ_C$ and from 33.1psu to 34.3psu in spring, from 14.5$^{\circ}C$ to 24.$2\circ_C$ and from 30.5psu to 34.1psu in summer, and from 14.$5\circ_C$ to 18.$6\circ_C$ and from 30.1psu to 34.0psu in autumn, respectively. 3) the distribution of watermass in the fishing ground varied largely each seasons, but a general tendency on the distribution was obtained. That is, in winter and spring the offshore water was distributed most widely and in summer the coastal and mixing water occupied the fishing ground but in autumn the mixing water prevailed. 4) variation of temperature and salinity were appeared between the surface and 20m layer in the sea aduacent to Naro Island. Therefore, in the summer the thermocline were made between surface and 20m layer with vertical gradients of 4.$0\circ_C$/7m.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.5
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• pp.565-570
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• 2013

Physiological studies on the salinity tolerance with respect to survival rate and body composition changes of the abalone Haliotis discus hannai were conducted by rapidly changing the salinity in an indoor rearing system. The survival rate of the control (35 psu), 30 and 25 psu groups at $15^{\circ}C$ was 100%. The survival rate of the 20 psu group was $35{\pm}5.0%$. Survival rate of the 15 psu group was 0%. At $15^{\circ}C$ and 35, 30, 25, 20, 15 psu, the moisture contents of abalone muscle were $82.1{\pm}0.7$, $82.5{\pm}0.7$, $84.9{\pm}0.5$, $86.9{\pm}0.3$ and $86.6{\pm}0.4%$, respectively. Crude lipid contents were $0.47{\pm}0.03$, $0.47{\pm}0.03$, $0.47{\pm}0.09$, $0.77{\pm}0.09$ and $0.63{\pm}0.03%$, respectively and crude ash contents were $1.30{\pm}0.12$, $1.33{\pm}0.15$, $1.13{\pm}0.23$, $1.87{\pm}0.15$ and $1.40{\pm}0.31%$, respectively. At salinity below 20 psu, these values increased compared with the control. The general components of abalone muscles significantly increased below 20 psu, while amino acid composition showed no significant difference with salinity and water temperature.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.1
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• pp.12-19
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• 2005

This study investigated the productivity and growth of copepods, Sinocalanus tenellus 2 strains, Paracyclopina nana 3 strains, Apocyclops royi 2 strains, Tachidius triangularis 1 strain and Tigriopus sp. 1 strain at different temperatures $(24,\;28\;and\;32^{\circ}C)$ under 15 psu and various salinities (10, 20, 30 psu) at $28^{\circ}C$ for an individual culture. And the mass culture was carried out to evaluate the production of these copepods in the 500 mL vessel. For an individual culture of copepod, survival period of a brood female tended to increase with the decrease of temperature, but was not significantly influenced by salinity. With an increase of temperature and a decrease of salinity, maturation period of nauplii in all species tended to decrease. Total nauglii production from a broodo female at $24^{\circ}C$ was higoher than that from a brood .female at either 28 or $32^{\circ}C$ for S. tenellus and at $32^{\circ}C$ than either 24 or $32^{\circ}C$ for A. royi Haenam stram, but was not influenced by temperature for another copepods. And total nauplii production from a brood female at 10 and 20 psu tended to be higher than that from a brood female at 30 psu, expect for Tigriopus sp. and P. nana Hwajinpo strain. Total nauplii production from a brood female was the highest in Tigriopus sp. (350 nauplii) at $28^{\circ}C$ under 20 psu, followed by A. royi Haenam strain (187 nauplii) at $32^{\circ}C$ under 15 psu and P. nana Hwajinpo strain (152 nauplii) at $24^{\circ}C$ under 10 psu. In the mass culture of each copepod, P. nana was the most productive (26.6 mg dry weight) and this value was two and four times higher than productivity of Tigriopus sp. and A. royi, respectively.

• Journal of fish pathology
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• v.22 no.3
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• pp.293-303
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• 2009

Effects of stress on the low salinity stress were examined in the pacific abalone Haliotis discus discus. Changes in survival rate, hemolymph count, antioxidant enzyme activities (catalase: CAT and superoxide dismutase: SOD), respiratory burst activity, phenoloxidase activity, lysozyme activity and expression of heat shock protein 70 (HSP70) mRNA were measured 0, 3, 6, 12, 24 or 48hours after low salinity treatment with 25, 30, 33 and 35 psu. Survival rates of pacific abalone were 100% at 33 and 35 psu, but 93 and 97% at 25 and 30 psu for 48 hours, respectively. Hemolymph counts decreased in the time elapsed-dependent way at all of the experimental groups. At low salinity, 25 and 30 psu, SOD and CAT activity increased compared to the experimental group of 33 psu. Moreover, respiratory burst activities of the pacific abalone seemed to have no effect on low salinity stress at any experimental group. However, phenoloxidase activity is an important component of the defence against pathogen that was decreased in a reduction of salinity dependent way. Lysozyme activity also immediately reduced at 25 psu experimental group for 48 h. The HSP70 mRNA was weakly expressed at 33 psu, but strongly detectable at 25 psu experimental group. The HSP 70 mRNA expression in gill increased in the time elapsed-dependent way at 25 psu experimental group and then recovered at 48 h. These results suggest that low salinity stress give rise to inhibitory action of immune system as a result of the decrease of phenoloxidase and lysozyme activity in the pacific abalone, especially.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.6 no.2
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• pp.93-102
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• 2001

Biomass and species composition of phytoplankton and zooplankton along the salinity gradients in the Seomjin River estuary were investigated in March, July, September and November, 1999. The locations of sampling sites were set based on the surface salinity during each cruise rather than geographic locations. A total of 96 phytoplankton species were identified with 60 diatom species in the study area. The species number of green algae was relatively high in low salinity region while the number of dinoflagellates increased in high salinity areas. Monthly mean of phytoplankton biomass ranged from 183 cells/ml to 833 cells/ml. There was a diatom bloom of Skeletonema costatum in November at the 5-15 psu salinity region and sharp decrease of biomass in very low salinity region occurred in March. During the rainy season in July with the salinity less than 8.1 psu, the green algae dominated in most of the study areas. Chroomonas spp. were highly dominant in March and September and the biomass increased towards the high salinity regions. A total of 83 zooplankton taxa were identified with 72 marine and 11 freshwater taxa. Major group of marine form was copepods with34 taxonomic groups. Most freshwater taxa were aquatic insects. Threshold salinities of freshwater zooplankton appearance were 8.1 psu in July, 4.7 psu in September, and 0.2 psu in November. The number of taxa appeared and abundances of zooplanktons were minimal in the middle of salinity gradients in this estuary.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.411-418
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• 2013

The Saemangeum Reservoir was constructed in 2006 as a part of Saemangeum reclamation project in which desalination has been also carried out. The purpose of this study is to provide basic data for predicting changes in fish distribution in the Saemangeum area with respect to the desalination processand to identify current status of fish distribution along with salinity levels. Fish sampling and measurement of salinity were conducted 4 times from February to October in 2012 at 10 sites within the Saemangeum Reservoir. A total of 71 species classified into 34 families were confirmed, and dominant and subdominant species were Thryssa kammalensis (16.1%) and Konosirus punctatus (12.8%), respectively. Saltwater fishes were still dominant at the Saemangeum Reservoir. Study sites were classified into four groups (A=St. 1, 6; B=St. 2, 3, 7, 8; C=St. 4, 9, 10; D=St. 5) by Bray-Curtis cluster analysis. Species composition of each group was closely related with salinity level: group A corresponding to 0.2 psu, B to 14.2 psu, C to 19.5 psu, and D to 23.5 psu.