Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Properties of Semen and Sperm Motility of Black Porgy Acanthopagrus schlegelii Acclimated in Freshwater

담수순화 감성돔 Acanthopagrus schlegelii의 정액특성 및 정자운동성

  • Jeong, Min- Hwan (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Chang, Young-Jin (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University)
  • 정민환 (국립수산과학원 양식관리과) ;
  • 장영진 (부경대학교 해양바이오신소재학과)
  • Received : 2011.02.17
  • Accepted : 2011.04.04
  • Published : 2011.06.30
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Abstract

The comparison of the chemical properties of semen of black porgy Acanthopagrus schlegelii long-term acclimated reared in freshwater (BFW) and seawater (BSW) with sperm activity of salinity and ion composition. The chemical properties of seminal plasma on BFW of the factors that most there was not significant difference in the BSW. However, osmolality in seminal plasma of BFW and BSW was $307.0{\pm}4.6$ and $337.3{\pm}10.1$ mOsm/kg, respectively, where BFW showed significant lower concentration in contrast to BSW. Salinity effect on sperm motility of BFW and BSW in 0 psu solution, no sperm motility was observed, whereas in 10 psu solution, both BFW and BSW sperms showed low motility and short time post sperm activation. However, diluted in 20 and 32 psu solutions, highest motility and long time post sperm activation were observed in BFW and BSW sperm. SAI of BFW and BSW varied in depend on the osmolality regardless of ion kind and it showed the highest value in the similar osmolality of artificial seawater (956 mOsm/kg). Accordingly, even in sperm released from BFW, factors initiating sperm motility are determined by osmolality.

장기간 담수순화 사육한 감성돔 Acanthopagrus schlegelii(BFW) 정액의 화학적 특성과 염분 및 이온조성에 따른정자활성을 해수사육 감성돔(BSW) 정액과 비교하였다. BFW 정장의 화학적 특성은 대부분의 요인에서 BSW 정장과 차이를 보이지 않았으나, 삼투질농도는 각각 $307.0{\pm}4.6$, $337.3{\pm}10.1$ mOsm/kg으로 차이를 보였다. 염분에 따른 BFW 및BSW의 정자운동성는 0 psu에서는 운동성이 관찰되지 않았으나, 10 psu에서 낮은 운동성과 짧은 정자 운동지속시간을 보였다. 그러나 20, 32 psu에서는 높은 운동성과 긴 정자 운동지속시간 보였다. BFW와 BSW 정자의 SAI는 이온의 종류와는 상관없이 삼투질농도에 의존하여 변화하였으며, 인공해수와 비슷한 농도에서 높았다. 결론적으로 장기간 담수에서 사육한 감성돔의 정자의 운동개시요인은 환경수의 삼투질농도가 좌우하는 것으로 판단된다.

Keywords

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