• KSBB Journal
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• 제26권6호
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• pp.483-490
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• 2011

Bioactive peptides (BAP) showed excellent cosmetic activity than bio-materials such as caffeic acid (CA), gallic acid (GA), and nicotinic acid (NA). Caffeoyl tripeptide-1 (CT-1) is a BAP that is stabilized with Gly-His-Lys (GHK) tripeptide and CA by using Fmoc solid phase peptide synthesis. Digalloyl tetrapeptide-19 (DT-19) is stabilized by combining Lys-Glu-Cys-Gly with GA and nicotinoyl tripeptide-1 (NT-1) is synthesized by GHK and NA. According to experiments, CT-1 has an excellent anti-oxidant function even with a very small amount of 10 ppm CT-1. DT-19's tyrosinase inhibition activity has the better effect of about 28.57% in 0.01% and 33.33% in 0.005% of concentration and about 7.89% in 0.001% concentration than vitamin-C. In addition, NT-1 is safer than the NA. Almost BAPs like pal-KTTKS, acetyl hexapeptide, and copper tripeptide-1 have the anti-wrinkle effect while DT-19 and NT-1 are applicable for potential BAPs focused on the whitening effect. The three kinds of BAPs like CT-1, DT-19, and NT-1 consisting of amino acids are safe to the skin, and have more excellent stability than bio-materials which are found to be unstable and cause skin irritation. Due to the high biological activity of BAP in the field of skin care, its utilization will increase constantly.

• 식물조직배양학회지
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• 제21권2호
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• pp.117-121
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• 1994

쇠비름(portuaaca oleracea L.)의 줄기절편체로부터 캘러스유도를 위한 적정배지는 BAP 0.1 + 2,4-D 1.0 mg/L., 30g/L sucrose 및 10 g/L agar를 첨가한 MS배지로 나타났으며, 액체배양에 의한 세포의 증식에는 BAP 0.1 + 2,4-D 0.5mg/L가 함유된 MS배지가 효과적이었다. 장시간 건조처리는 세포는 활력과 재생 및 재분화율이 강하게 억제되나, 1시간 및 2시간동안의 단시간 건조처리한 세포의 재분화율은 각각 83및 80%로 나타나나 무처리 61%에 비해 높았다. 0.6% NaCl의 2일간 전처리는 세포의 재분화율에는 영향을 미치지 않으나, 세포활력 및 재생률에는 억제작용을 나타내었다. Sucrose 70및 100 g/L를 전처리한 쇠비름세포의 재분화율은 각각 80및 81%로 무처리에 비해 높았다.

• Journal of Plant Biotechnology
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• 제6권3호
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• pp.171-179
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• 2004

Hormonal requirements inducing different regeneration pathways with particular emphasis on somatic embryo-genesis in Alhagi graecorum were studied. While combination of 0.5 $\mu{M}$ 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 $\mu{M}$ 6-benzylaminopurine (BAP) and 5 $\mu{M}$ 1-naphthaleneacetic acid (NAA) in MS medium induced callus formation and callus maintenance from internodal explants, each alone or in combination with other induced distinct regeneration pathway. Adventitious bud formation was induced on MS medium supplemented with 2.5 $\mu{M}$ BAP. It was improved when 2.5 $\mu{M}$ BAP was used in combination with 5 $\mu{M}$ NAA. MS medium containing 0.5 $\mu{M}$ 2,4-D or 5 $\mu{M}$ NAA induced the formation of abnormal direct somatic embryos. While increase of 2,4-D concentration (1.125-9) resulted in the formation of viable embryogenic mass, increase of NAA did not change its effect. NAA should be used in combination with 2,4-D even at low concentration (0.5 $\mu{M}$) to form embryogenic mass. In A. gaecorum, the role of 2,4-D as trigger of somatic embryogenesis and BAP as trigger of adventitious bud formation was deduced, but for maximum yield certain auxin-cytokinin ratio should be applied. Embryogenic masses characterized by high water content, low peroxidase activity, and low number of peroxidase and glutamate oxaloacetate transaminase bands in comparison with calli obtained under conditions stimulating adventitious bud formation. The resulted differential gene expression, which could be detected by native-PAGE patterns, could be used as marker for organogenic pathway in A. graecorum.

• 아시안잔디학회지
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• 제8권3호
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• pp.137-147
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• 1994

We have established a high-frequency plant regeneration system via organogenesis from mature seed of Korean lawngrass(Zoysia japonica Steud.). The effects of 2,4-dichiorophenoxy acetic acid (2,4-D), 6-furfuryl amino purine (kinetin), $\alpha$-naphthaiene acetic acid (NAA), N6-benzyl amino pu-rine (BAP), and casein hydrolysate (CR) on cailus induction and multiple shoot formation on ex-posure to light were evaluated. Callus produced on the Murashige and Skoog (MS) medium containing 2,4-D and kinetin had high organogenesis potency. A single addition of 1.0 mg $L-^1$ 2,4-D significantly induced callus. Also, 1.0 mg L-$^1$ 2,4-D, with the addition of 0.1 mg $L-^1$ kinetin highly enlianced callus induction. The trend of cailus induction was also found on mediurn containing 0.1 mg $L-^1$ BAP with 1.0 mg $L-^1$2,4-D, and 1 g $L-^1$ CR with the addition of 1.0 mg $L-^1$ 2,4-D. However, NAA was no effective on callus formation. The growth of root was significantly high in the presence of 0.1 mg $L-^1$ kinetin compared to other concentrations. Over 2 mg $L-^1$ kinetin highly lengthened roots. Fresh weight of plantlet was highest on medium containing 0.1 mg $L-^1$ 2,4-D. Also, on medium containing 0.1 mg $L-^1$ BAP, fresh weight of piantlet was highly enhanced. BAP was significantly effective on multiple shoot formation, particularly when 2.0 mg $L-^1$ was added with 0.1 mg $L-^1$ 2,4-D. Callus induction and multiple shoot formation were achieved on MS basal medium containing 1.0 g $L-^1$ CH.

• 한국산림과학회지
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• 제86권3호
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• pp.391-398
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• 1997

참나무류의 효율적인 기내 번식법 개발을 위하여 상수리나무 유묘와 수형목을 재료로 액아를 배양하였다. 5가지 배지 가운데 WPM 배지에서 줄기 분화 및 생장이 가장 양호하였다. 싸이토키닌중 zeatin 3.0mg/L의 농도에서 줄기 분화 및 생장에 효과를 보였으나 대체로 BAP의 처리가 다경 줄기 분화 및 생장에 주효하였다. BAP와 zeatin의 공조처리는 다경줄기 유도 및 생장에 더욱 효과가 있었으며, 적정 농도는 BAP 0.5, zeatin은 0.05-1.0mg/L이었다. 기내발근은 1/2 GD 배지에 0.5mg/L IBA 처리로 90% 이상 발근되었고 인공 배양토에서 70% 이상 활착되었다. 유령 대목에 2회 접목된 27개 수형목 클론을 재료로 시험한 결과는 4개 클론만이 기내 증식의 가능성을 보였다. 재유령화된 수형목의 기내 번식은 모수령의 영향보다는 클론간의 차이에 크게 좌우되었다. 기내 반응이 우수한 수형목 클론은 증식후 90% 이상 발근되었다. 발근배지는 염류를 반감시킨 GD배지에 IBA 0.2-2.0mg/L 처리시 주효하였다. 발근묘는 인공 배양토에 이식하여 5개월 후 60% 이상 활착되었다.

• Journal of Plant Biotechnology
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• 제48권3호
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• pp.173-178
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• 2021

Basal callus formation and leaf abscission is a problem in clonal micropropagation. We have described an in vitro clonal propagation protocol of Dalbergia sissoo Roxb (shisham) 'FRI-14' in which AgNO₃ played important role not only in mitigating problem of leaf abscission and basal callus, but also improved shoot induction and multiplication. Best induction and shoot multiplication was obtained on MS media with 1.5 mg/l 6-BAP and 10 mg/l AgNO₃ and half-strength MS media with 0.5 mg/l 6-BAP, 2 mg/l AgNO₃ and 50 mg/l Adenine sulphate whereas best ex vitro rooting was obtained with 200 mg/l IBA in pulse treatment.

• Journal of Plant Biotechnology
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• 제6권3호
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• pp.193-197
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• 2004

In vitro multiplication of Curculigo orchioides was achieved by direct somatic embryogenesis in young leaf segments. Immature leaf segments of about 0.5 cm in length were cultured on MS medium supplemented with different concentrations of BAP (2-10 $\mu{M}$) or Kin (2-10 $\mu{M}$). Optimum response in terms of per cent cultures responding (89%) and the number of embryos per explant (16) were observed on MS medium supplemented with 8 $\mu$M BAP. The emergence of several somatic embryos on the adaxial side of the leaf segments was observed one month after the culture. Germinated somatic embryos were grown up to about 1.5 cm length before transferring to maturation medium. For maturation, the individual embryos were isolated and transferred to MS medium supplemented with BAP (5 $\mu{M}$) and NAA (0.5 $\mu{M}$). The plantlets emerged from the embryos were transferred to soil containing 1 peat: 1 sand with 90% success. The embryos were formed directly on the leaf segments without any callus phase. Direct regeneration of somatic embryos is important for the conservation of this endangered species, as rare somaclonal variants are likely to arise than from indirect regeneration.

• 한국작물학회지
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• 제46권6호
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• pp.464-467
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• 2001

황칠나무의 선발된 우량개체군의 집단재배시 우량해체간의 교잡에 의해서 형성된 종자를 증식하기 위한 기내배양체계를 확립하기 위해서 기내 발아, 증식, 발근에 적합한 식물생장조절제를 구명하기 위하여 본 실험을 실시한 결과는 다음과 같다. 황칠나무 종자의 무균발아에 적합한 배지로는 MS(Murasinge Skook)기본배지가 발아율이 높고 유식물체(plantlet)의 생장이 양호하여 알맞는 배지로 판단 된다. 기내에서 신초의 증식과 생육에 효과적인 식물생장조절제는 사이토키닌(BAP 0.1~l.0 mg/l)과 오옥신(NAA 0.5~1.0 mg/l)의 혼용처리가 효과적이었다. 또한 기내의 발근은 오옥신의 첨가에서 쉽게 유도되었으며, 그 중에서 NAA1.0 mg/l첨가에서 가장 양호하였다.

• 생물환경조절학회지
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• 제17권1호
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• pp.51-59
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• 2008

상추는 전형적인 광발아 종자로서 품종별, 광질 및 생장조절물질 등의 영향을 크게 받는다. 대부분의 품종은 백색광과 적색광 하에서는 85% 이상의 발아율을 보였으나, 청색광에서는 발아세가 저하되었으며, 초적색광에서는 거의 모든 품종에서 발아가 되지 않아 광에 의한 발아억제효과가 인정되었다. 'Cheongguangcheongchima'는 초적색광 하에서도 78%, 'Okdol'은 63%, 'Manchudaecheongchima'도 48% 이상의 발아율을 나타내어 이들 품종은 광에 둔감한 품종으로 분류되었다. 초적색광의 발아 불량조건 하에서 BAP와 kinetin은 종자발아 및 정상유묘출현율을 촉진시킨 반면, ethephon은 효과적이지 못하였다. BAP, kinetin, TDZ, zeatin과 같은 cytokinin류는 $50mg{\cdot}L^{-1}$ 이하로 처리하여 초적색광에 의한 발아억제효과가 상쇄되었으나, IBA, 2,4-D, NAA의 auxin류는 별다른 극복효과를 나타내지 않았다.

• BMB Reports
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• 제32권1호
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• pp.51-59
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• 1999

The Korean radish cationic peroxidase (KRCP) promoter, comprising nucleotides -471 to +704 relative to the transcriptional initiation site, was fused to the GUS gene and transformed to tobacco BY-2 cells. We examined how auxin (2,4-dichlorophenoxyacetic acid, 2,4-D), cytokinin (6-benzylaminopurine, BAP), gibberellic acid ($GA_3$), abscisic acid (ABA), methyl jasmonate (MeJA), and phosphatidic acid (PA) affect the GUS expression in the presence or absence of 2,4-D in a modified LS medium. Exogenous 2,4-D or BAP greatly decreased the GUS expression regulated by the KRCP promoter in a modified LS medium containing 0.2 mg/l 2,4-D. $GA_3$ increased the GUS expression and ABA completely reduced the inductive effect of $GA_3$. The GUS expression was also increased dose-dependently by plant defense regulators, MeJA and PA. In contrast to the above results, auxin deprivation from the modified LS medium increased the GUS expression after treatment with exogenous 2,4-D whereas BAP still greatly decreased the GUS expression dose-dependently. $GA_3$ or MeJA slightly decreased the GUS expression. The data suggest that auxin deprivation changes the sensitivity of the suspension cells to exogenous chemicals and that the regulation of the KRCP promoter by 2,4-D, $GA_3$, and MeJA is dependent on auxin, whereas the regulation by BAP is not. This study will be valuable for understanding the function and expression mode of the Korean radish cationic peroxidase in Korean radish.