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Induction of Apomixis by Chemical Mutagen Treatment and Ovule Development in Inbreed lines of Corn (옥수수 자식계통들에서 화학적 돌연변이 유발성질 처리에 따른 apomixis 유발과 배주발생)

  • 이호진;최근진;김태훈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.5
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    • pp.476-485
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    • 1992
  • M1 plants which were produced from seed soaking in chemical mutagen, EMS or NaN$_3$, appeared wide morphorogical variations such as dwarf, albino, twisted leaf, white streaked leaf, and purpled stem. In mutants of reproductive organs, there were monoecious plants such as female-flower plant and male-flower plant, multiple spikes, and steriled plants among M1 plants. Also, barren stalk was increased significantly in M1 plants. Ear bagging at ear initiation stage prevented seed set on cob in normal plants. In spite of ear bagging, M1 plants which had cobs with seed set was 3.9-11.2% of stalks developed from seeds soaking with mutagens, but only three or four kernels could be matured on a cob. Ear bagging after mutagen injection into initiating ear produced 5.1-10% in cobs with seed set, but only 1.7-6.3 kernels could be matured. Cobs removed silk at four hours after artificial pollination increased the rate of cobs with seed set to 27%. Microscopic observation confirmed that ontogeny of kernels matured from ear bagging and mutagen treatment would be both adventitious and diplosporous apomictic reproduction. Chromosome set of M2 seedling was found to be diploid type in chromosomal counting of root tip. As M$_2$ plants showed an uniform appearence within each lines and their CV of plant height were ranged 4-6% in each lines, we concluded that they were apomictic progeny. But we could not find any marker traits combined with apomixis.

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Spawning, Hatching and Larval Growth of Red and Black Clownfish Amphiprion melanopus (Red and Black Clownfish Amphiprion melanopus의 산란, 부화 및 자어성장)

  • Kim, Suk-Ree;Hur, Sung-Bum
    • Journal of Aquaculture
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    • v.20 no.4
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    • pp.239-247
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    • 2007
  • We studied spawning, hatching and larval growth of Amphiprion melanopus under laboratory conditions. Each time a fish spawned about 440 to 650 eggs. The eggs were hatched 9 days later. Nest colour of the eggs changed from clear yellow orange to greyish violet with processing of embryogenesis. With regard to spawning according to the substrates, the fish prefered red clay pot and ceramic pot to volcanic scoria and live rock. Hatching rate (%) of the eggs on the former substrates was significantly higher than that on the latter. The number of eggs varied with the food stuff supplied to the fish. The fish fed Tetra bits (commercial diet), meat of short necked clams and mussels, and astaxanthin showed significantly higher fecundity with 638 eggs than that fed only Tetra bits (477 eggs) or Tetra bits with meat of shellfish without astaxanthin ($549{\sim}553\;eggs$). Higher temperature ranging from 24 to $30^{\circ}C$ induced shorter incubation period and smaller total length, but higher hatching rate. The optimum salinity and L:D cycle with $60\;{\mu}mol$ photon $m^{-2}s^{-1}$ for hatching were 33 psu and 16:8, respectively. The newly hatched larvae about 4.6 mm in total length grew to 7.5 mm in one week, 18.8 mm in a month and 37.9 mm in 70 days after hatching.

A Very Early-Maturing Italian Ryegrass (Lolium multiflorum Lam.) New Variety, 'Green Farm' for Double Cropping System (이모작 작부체계에 적합한 극조생종 이탈리안 라이그라스 신품종 '그린팜'의 품종 특성)

  • Ji, Hee-Chung;Lee, Sang-Hyun;Yoon, Sei-Hyung;Kim, Ki-Yong;Choi, Gi-Jun;Park, Hyung-Soo;Park, Nam-Gun;Lim, Young-Chul;Lee, Eun-Sup
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.1
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    • pp.9-14
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    • 2011
  • This experiment was carried out to breed a very early maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 2009 to 2010. A new variety, 'Green farm' is a diploid variety with green in leaf color and has erect and semi-erect growth habit in late autumn and early spring, respectively. 'Green farm' was on 28 April in heading date as a early-maturing variety. 'Green farm' was also wider by 0.4 mm in flag leaf width, longer by 0.8 cm in flag leaf length and shorter by 5 cm in plant height than those of control variety, 'Florida 80', respectively. 'Green farm' was thicker in stem thickness and stronger in winter hardness than those of the 'Florida 80'. Dry matter (DM) yield (11,790 kg/ha) of 'Green farm' was similar to that of 'Florida 80'. In vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN) and crude protein (CP) of 'Green farm' were 68.7, 63.3 and 10.3% which are 1.9, 1.7 and 0.6% higher than those of 'Florida 80', respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of 'Green farm' were 32.5 and 54.6% which are 2.2 and 4.3% lower than those of the 'Florida 80', respectively.

Root zone environments in two cropping system within a year for Kyoho grapes (포도 '거봉'의 2기작 재배에서 근권환경 특성)

  • 오성도;김용현
    • Journal of Bio-Environment Control
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    • v.6 no.4
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    • pp.235-241
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    • 1997
  • This study was performed to investigate the behaviour of root zone environments under the control of soil temperature and tension of soil moisture near the root Bone of 'Kyoho' grapes tree grown on restricted root zone system in plastic greenhouse. Maximum diurnal air temperature inside plastic greenhouse ranged between 25.1 and 32.7$^{\circ}C$, and the average of nocturnal air temperature inside plastic greenhouse maintained at 18$^{\circ}C$ in winter season. Also the minimum diurnal relative humidity ranged between 50 and 55%, and the maximum nocturnal relative humidity ranged between 84 to 87%. At a depth of 15cm from soil surface, the average soil temperature maintained at 25.6$^{\circ}C$ for under-ground heating, and appeared to 17.4$^{\circ}C$ for unheated condition. Although the tension of soil moisture just after irrigation sharply decreased to pF 1.5, the tension of soil moisture at the depth of 15cm maintained at pF 2.0~2.2. It is suggested that the tension of soil moisture at the depth of 15cm might be used as the standard for the determination of irrigation set point. Effective drainage system is needed to prevent the spindly and succulent growth of vine trees grown in restricted root zone system.

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Characteristics of mulberry cultivar Shimheung (Morus alba L.) for mulberry fruit production (오디 생산용 뽕나무 품종 '심흥'의 특성)

  • Sung, Gyoo Byung;Kim, Hyun-Bok;Kang, Pil Don;Kim, Kee Young;Ji, Sang Duk
    • Journal of Sericultural and Entomological Science
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    • v.51 no.2
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    • pp.130-136
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    • 2013
  • We bred a mulberry cultivar named Shimheungppong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Wanju, and Jinju) for six years from 2004. This is tetraploidy variety belonging to (Morus alba L.) selected among mulberry genetic resources preserved in Suwon. Shimheungppong was high yielding cultivar in fruit productivity by 5% compared to control cultivar 'Chungilppong(Morus alba L.)' for four years. Although Shimheungppong was a little lower in sugar content of mulberry fruits, it was bigger than that of Chungilppong. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

Characteristics of mulberry cultivar 'Sangmaru' (Morus alba L.) for mulberry fruit production (오디 생산용 뽕나무 품종 '상마루'의 특성)

  • Sung, Gyoo Byung;Kim, Yong Soon;Kim, Kee Young;Ji, Sang Duk;Kim, Hyun-bok
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.110-117
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    • 2015
  • We bred a mulberry cultivar named Sangmaru, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places (Suwon, Cheongju, and Jangseong) for seven years from 2008. This is tetraploidy variety belonging to Morus alba L. made by colchicine treatment on growing point of 'Chungil (Morus alba L.)' winter buds. Sangmaru was high yielding cultivar in fruit productivity by 6% compared to control cultivar 'Chungil (Morus alba L.)' for five years. Although Sangmaru was similar in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

Characteristics of Mulberry Cultivar "Sangberry"(Morus alba L.) for Fruit Production (오디 생산용 뽕품종 "상베리"의 특성)

  • Sung, Gyoo Byung;Kim, Hyun-Bok;Kang, Pil Don;Kim, Ki Young;Ji, Sang Duk
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.171-177
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    • 2012
  • We bred a mulberry cultivar named Sangberry, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four places(Suwon, Kongju, Jangseong and Sangju) for six years from 2005. This is tetraploidy variety belonging to(Morus alba L.) made by colchicine treatment on growing point of winter buds. Sangberry was high yielding cultivar in fruit productivity by 70% compared to control cultivar "Chungilppong(Morus alba L.)" for four years. Although Sangberry was a little lower in sugar content of mulberry fruits, it contains more bioactive materials like C3G, rutin and amino acids than Chungilppong. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

Development and Characterization of New Tall fescue Variety 'Greenmaster4ho' (톨 페스큐 신품종 '그린마스터4호'의 품종 특성 및 수량성)

  • Lee, Ki-Won;Ji, Hee Jung;Choi, Gi Jun;Kim, Ji Hye;Song, Yowook;Woo, Jae-Hoon;Lee, Sang-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.280-285
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    • 2018
  • A new variety of tall fescue (Festuca arundinacea Schreb.) named 'Greenmaster4ho' was developed during the cultivation year 2010-2017 at Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Korea. In order to produce this new variety, 5 superior tall fescue lines including 12XFa07, 12XFa15, 12XFa24, 12XFa46, and 12XFa48 were polycrossed. The new variety Greenmaster4ho was evaluated in field test (Cheonan, Pyeongchang, Jeju, and Jinju) for determining the agronomic growth characteristics and forage production capability during 3 years (2015-2017). The dry matter yield (16,236 kg/ha) of Greenmaster4ho was 5 % higher than Fawn, but the nutritive value as forage crops was not significantly different with Fawn. Development of new tall fescue variety with excellent adaptability to changing unfavorable environment would be useful for forage cultivation and yield in Korean environment.

Application of AFLPs to Phylogenetic Analysis of Aegilops (AFLPs에 의한 Aegilops의 계통발생학적 재평가)

  • Park, Yong-Jin;Shim, Jae-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.790-799
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    • 1997
  • Aegilops genus is known to include the donor species of the Band D genome of the bread wheat(ABD). An effort to establish a better strategy for phylogenetic relationships about Aegilops polyploids by AFLPs(Amplified Fragment Length Polymorphisms) was conducted using the 19 Aegilops sPP. and T. aestivum. The 207 polymorphic bands from the amplified products on the 6% acrylamide denaturing sequencing gels were obtained with the 7 AFLP primer combinations, and used to account for the genetic similarities and cluster analysis using NTSYS program. According to the genome analysis, the $M^h$-genome of Ae. heldreichii was estimated as an intermediate genome between the M-genome of Ae. comosa and N-genome of Ae. uniaristata and supposed to be incorporated in the establishing process of UM-genome as a possible diploid donor. And Ae. ventricosa(DN) was more close to Ae. umbellulata(U) than Ae. squarrosa(D). The close relationship between Ae. squarrosa and T. aestivum was perceived as a diploid donor of D-genome. As for the polyploid species, hexaploid Ae. triaristata was more closely related to Ae. columnaris rather than tetraploid Ae. triaristata. The clustered groups were, basically same to the previous Gihara's sections based on phenotypes and pairing analysis of interspecific hybrids. AFLP was evaluated as an efficient and powerful method in the genome evaluation of closely related species.

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Growth Characteristics and Yield of a New Italian Ryegrass (Lolium Multiflorum Lam.) Variety, 'Earlybird' (이탈리안 라이그라스 신품종 '얼리버드'의 생육특성과 수량성)

  • Jae Hoon Woo;Bo Ram Choi;Chang-Woo Min;Ki-Won Lee
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.44 no.2
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    • pp.127-132
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    • 2024
  • This experiment was conducted to breed a very early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.) at the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea, from 2022 to 2023. The new variety, named "Earlybird," is a diploid variety characterized by green leaf color, a semi-erect growth habit in late autumn/fall, and an erect growth habit in early spring. With a heading date of April 30, Earlybird is categorized as a very early-maturing variety. Compared to the "Kowinearly" control variety, Earlybird's flag-leaf width is wider by 0.2 mm, its flag-leaf length is longer by 1.4 cm, and its plant height is greater by 10.5 cm. Additionally, Earlybird's ear length is 1.4 cm longer than Kowinearly's, and it exhibits lodging resistance. Although the dry matter yield of Earlybird (7,469 kg/ha) is smaller than that of Kowinearly, the difference is not statistically significant. The total digestible nutrient and crude protein content of Earlybird are 63.5% and 8.6%, respectively, which are higher than those of Kowinearly by 1.8% and 0.3%, respectively. The neutral detergent fiber and acid detergent fiber content of Earlybird are 56.9% and 32.0%, respectively, which are lower than those of Kowinearly by 2.2% and 2.3%, respectively.