• Journal of Microbiology and Biotechnology
• /
• 제7권5호
• /
• pp.360-362
• /
• 1997

A transposon Tn5 mutant of Rhodobacter sphaeroides 2.4.1 was isolated for its impaired ability of growth on minimal medium containing ${\beta}$-hydroxybutyric acid as a sole carbon source. The mutant, R. sphaeroides S7 showed approximately 6-fold decrease in ${\beta}$-hydroxybutyrate dehydrogenase activity compared with that of wild type. In R. sphaeroides S7 the Tn5 was located in DNA region corresponding to a 4.2-kb EcoRI DNA fragment of R. sphaeroides 2.4.1 chromosome.

• 한국응용곤충학회:학술대회논문집
• /
• 한국응용곤충학회 2001년도 추계 합동 심포지엄 및 학술발표대회
• /
• pp.54-54
• /
• 2001

• Fisheries and Aquatic Sciences
• /
• 제15권2호
• /
• pp.125-135
• /
• 2012

This study was carried out to understand the correlation between phylogenetic relationships based on 18S rDNA sequences and growth by salinity of Chlorella-like species. The 18S rDNA sequences of 71 Chlorella-like species which were mainly collected from Korean waters were analyzed. The 18S rDNA sequences of Chlorella-like species were divided into three groups (group A, B and C) and group B was further divided into three subgroups (subgroup B-1, B-2 and B-3). Thirty-seven Chlorella-like species in group A grew well at high salinity (32 psu) but the other groups grew well in freshwater. The sequence identities of the species in group A and B were 97.2-99.5%, but those of 6 species in group C ("Chlorella" saccharophila), which contained group I intron sequences region were 75.0-75.4%. Two representative species of each group were cultured at different salinities (0, 16 and 32 psu) to examine the correlation between the molecular phylogenetic groups and the phenotypic characteristics on cell growth and size by different salinities. The size of cell cultured at different salinities varied according to the species of each molecular phylogenetic group. The size of "Chlorella" saccharophila in group C was bigger and more obviously elliptical rather than that of the other Chlorella-like species. Considering the results on molecular and phenotypic characteristics, the group A and B belonged to Chlorellaceae, but group C was distinctly different from them.

• The Korean Journal of Ecology
• /
• 제26권6호
• /
• pp.307-312
• /
• 2003

본 연구는 2000년 7월에 전남 장흥군에서 채집한 불가사리의 장내에 존재하는 종속영양세균의 다양성에 대해서 알아보았다. 불가사리 장내에 존재하는 균체수를 측정하였으며, 순수 분리된 균주를 대상으로 16S rDNA 증폭기법을 이용하여 세균의 다양성을 조사하였다. 불가사리 장내에 분포하는 종속영양세균의 균체수는 8.65${\pm}$0.65${\times}10^3\;dfu\;g^{-1}$이었다. 29 균주의 세균이 순수 분리되었으며, 그 중 그람양성 세균은 분리된 균주의 59% (17균주)를 차지하였다. 불가사리 장내에서 분리된 균주는 Bacillus속, Microbacterium 속, 그리고 Marinobacter 속 등이 우점이었으며, 이외에도 Staphylococcus 속, Psychrobacter 속, Paracoccus 속, Erythrobacter 속, Zoogloea 속, Kocuria 속과 Arthrobacter 속 등이 포함되었다. 분리된 균주 가운데 Bacillus 속에 속하는 8균주 중 3균주는 type strain과 97% 이상의 유사도를 보인 반면, 5 균주는 유사도가 90%로 비교적 낮은 유사도를 보여 현재까지 알려지지 않은 신종일 가능성이 높다고 하겠다.

• Journal of Microbiology and Biotechnology
• /
• 제10권5호
• /
• pp.643-650
• /
• 2000

A new aniline-utilizing microorganism, strain HY1 obtained from an orchard soil, was characterized by using the BIOLOG system, an analysis of the total cellular fatty acids, and a 16S rDNA sequence. Strain HY1 was identified as a Burkholderia species, and was designated Burkholderia sp. HY1. GC and HPLC analyses revealed that Burkholderia sp. HY1 was able to degrade aniline to produce catechol, which was subsequently converted to cis,cis-muconic acid through an ortho-ring fission pathway under aerobic conditions. Strain HY1 exhibited a drastic reduction in the rate of aniline degradation when glucose was added to the aniline media. However, the addition of peptone or nitrate to the aniline media dramatically accelerated the rate of aniline degradation. A fatty acid analysis showed that strain HY1 was able to produce lipids 16:0 2OH, and 11 methyl 18:1 ${\omega}7c$ approximately 3.7-, 2.2-, and 6-fold more, respectively, when grown on aniline media than when grown on TSA. An analysison the alignment of a 1,435 bp fragment. A phylogenetic analysis of the 16S rDNA sequence based on a 1,420 bp multi-alignment sowed of the 16s rDNA sequence revealed that strain HY1 was very closely related to Burkholderia graminis with 95% similarity based that strain HY1 was placed among three major clonal types of $\beta$-Proteobacteria, including Burkholderia graminis, Burkholderia phenazinium, and Burkholderia glathei. The sequence GAT(C or G)${\b{G}}$, which is highly conserved in several locations in the 16S rDNA gene among the major clonal type strains of $\beta$-Proteobacteria, was frequently replaced with GAT(C or G)${\b{A}}$ in the 16S rDNA sequence from strain HY1.

• Journal of Radiation Protection and Research
• /
• 제23권2호
• /
• pp.89-96
• /
• 1998

뇌하수체 분비호르몬인 FSH의 방사선방어 효능을 조사하고자 생후 3주된 미성숙 생쥐에 $\gamma$ 선을 전신조사하거나(R), 전신조사 후 FSH 5 IU를 복강주사(RF)하였다. 대조군은 동량의 식염수를 복강주사하거나(C) 혹은 FSH를 복강주사(F)하였다. 0시간, 6시간, 12시간 1일, 2일, 4일, 그리고 8일 후에 각 군별로 5마리씩 경추파열로 도살한 후 난소를 채취하였다. 난소는 neutral buffered formalin에 고정한 후 통상적인 표본조직을 만들었다. HE 염색을 기본으로 면역조직화학염색을 비교하였으며 전체 DNA를 추출하여 agarose gel 전기영동을 실시하여 DNA fragmentation의 정도를 알아보았다. 방사선 조사군인 R군과 RF군에 있어서 6시간에서 12시간에 apoptosis를 반영하는 면역조직화학적 염색률이 높게 나타났으며, 8일 경과시 분열세포의 존재비를 반영하는 염색률이 높게 나타났다. 또한 DNA fragmentation의 정도를 보면, 모든 실험군에서 185bp의 DNA ladder가 시간에 따라 증가하는 경향을 보였다. 370bp의 DNA는 R군의 경우 6시간에 나타났으며 1일 이후 감소하였다. RF군의 경우 12시간에 나타나 1일 이후 감소하였다. F군에서는 370bp가 보이지 않았다. 본 실험의 결과, 강소형성 난포가 피폭되는 경우 4일 이후 8일에 이르러 난소내에서 완전히 소멸되고, 8일에 새로운 원시난포가 성장하는 것이 확인되었다 FSH는 방사선에 피폭된 난포의 퇴화를 지연 혹은 억제시키는 방사선방어 효과가 있었으며, 방사선에 의한 난포의 퇴화는 과립세포의 apoptosis를 매개로 하여 일어남을 알 수 있었다.

• 한국자원식물학회지
• /
• 제19권1호
• /
• pp.161-168
• /
• 2006

제주도에 자생하는 부채 선인장인 백년초의 기원 규명을 목적으로 ITS primer를 이용하여 685 bp의 ITS 영역을 분리하였다. ITS 영역의 염기서열을 분석한 결과 18S rRNA의 길이는 54 bp, 26S rRNA는 55 bp, ITS1은 193 bp, ITS2는 220 bp로 구성되어 있었다. 백년초 ITS 영역은 기존에 보고된 Cucurbitoideae 식물들의 ITS 영역에 비하여 ITS2 스페이서 영역의 239-254 bp보다는 다소 짧았다. 그러나 이들 스페이서 영역의 GC 함량은 백년초의 경우 ITS1은 66.8%, ITS2의 경우에는 67.7%로 Cucurbitoideae 식물들에서 보다 높은 GC 함량을 나타내었다. 백년초 선인장의 rDNA 영역에 가장 높은 상동성을 나타낸 것은 같은 Opuntioideae에 속하는 Pereskiopsis porteri(L78037)로 95%의 유사도를 나타내었다. 백년초 rDNA Clustal W 프로그램을 이용하여 유연관계를 조사한 결과 같은 Opuntioideae에 속하는 Pereskiopsis porteri(L78037)와 같은 cluster로 분리되었다.

• Journal of Life Science
• /
• 제11권2호
• /
• pp.74-80
• /
• 2001

The nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of ribosomal DNA (rDNA), and the 5.85 rRNA gene, have been determined for 13 strains of dinoflagellates in order to analyze the phylo-genetic relationship. The DNA sequences contained considerable variation in the ITS regions, but little in the 5.85 rDNA. In addition, the ITS1 was more variable than the ITS2 in all species examined. The nucleotide length of this region varied from 519 bp to 596 bp depending on the taxa. The investigated taxa were divided into three large groups based on the ITS length, i. e., a group with short ITS region (A. fraterculus and Alexandrium sp.), a with ITS region group (P. micans, P. minimum and P. triestinum) and a with ITS region group (G. impudicum, C. polykrikoides, G. sanguineum, G. catenatum and H. triquetra). The relationship between nucleotide length of ITS1 and that of ITS2 was negative, whereas G+C content and nucleotide length showed positive correlation. In phylogenetic analyses producing NJ trees, the topology was similar cluster and clearly divided the taxa into three groups based on 5.8S rDNA that were similar to those based on morphological characteristics. In particular, G. impudicum was more closely related to G. catenatum than to C. polykrikoides using phylogenetic analysis. From this study, we chew that the length of ITS region contributes to discriminate Korean harmful algal species and ITS analysis is a useful method for resolving the systematic relationships of dinoflagellates.

• Ocean Science Journal
• /
• 제41권4호
• /
• pp.315-318
• /
• 2006

Three Synechococcus strains were isolated from seawater near the Ieodo Ocean Research Station (IORS), and their 16S rDNA genes and the internal transcribed spacer (ITS) between the 16S and 23S rRNA genes were sequenced to investigate their phylogenetic relationships. Phylogenetic trees based on the 16S rDNA and ITS sequences showed that they clustered in the main MC-A Synechococcus group (subcluster 5.1), but formed branches differentiating them from the described clades. As the IORS is located in an area affected by diverse water masses, high Synechococcus diversity is expected in the area. Therefore, the IORS might be a good site to study the diversity, physiology, and distribution of the Synechococcus group.

• 미생물학회지
• /
• 제23권2호
• /
• pp.115-121
• /
• 1985

Lactobacillus casei에 감염하는 독성 phage중 각 분류꾼을 대표하는 5종의 phage (J1, TK93, K1, PD 5 빛 CP 1)와 l 종의 용원 phage (${\phi}$ 1043) 의 핵산의 특성을 바교 검토하였다. 실험한 6 종의 phage는 모두 double S stranded DNA를 갖고 있였으며 J1. TK93, K1 및 ${\phi}$ 1043 DNA의 크기는 약 42Kb, PD5 와 CP1 DNA는 140K Kb정도로 서로 비슷하였다. EcoR 1으로 절단시 J1, TK93, K1, PD5, CP1 및 ${\phi}$1043은 각각 13, 13. 11, 14, 14와 12개의 절편을 갖는 특징적인 절단양식을 보여주었다. J1, TK93 및 ${\phi}$ 1043 DNA에는 cohesive end가 존재 하였고 K1, PD5 빛 CP1 DNA에는 없는 것으로 사료되었다. J1과 TK93 DNA의 제한효소 지도를 작성하여 비교 검토하였으며 이상의 결과로부터 진화적 유연관계를 검토하였다.