• 제목/요약/키워드: 50's and 60's

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Hydrolysis of Anchovy (Engraulis Japonicus) Homogenate with Salting and Digestion Time (가염 및 분해기간에 따른 멸치의 가수분해)

  • Choi, Im-Soon;Kim, Gu-Young
    • Korean Journal of Food Science and Technology
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    • v.16 no.1
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    • pp.23-28
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    • 1984
  • Anchovy homogenates with or without salt were autolyzed at various pH and temperature conditions. In the initial hydrolysis during 20 hours, the highest autolysis of anchovy homogenate was achieved at pH 4 and $50^{\circ}C$. However, the addition of 20% salt changed the optimum condition to pH 6 and $50^{\circ}C$. When the digestion time was prolonged to 8 days, the most favorable temperature for the autolysis of salted anchovy was lowered to $40^{\circ}C$ compared with $50^{\circ}C$ of initial hydrolysis while the optimum pH was unchanged. Under the best conditions described above, 60.5% of anchovy nitrogen was converted to TCA-soluble nitrogen in 20 hr-incubation without salting, but it was reduced to 49.8% with salting. In the 8 days hydrolysis of salted anchovy, as much as 83.1% of total nitrogen was transformed into TCA-soluble nitrogen. Slight increase in the degree of hydrolysis up to 89.6% was occurred during subsequent ripening period of 52 days at ambient temperature.

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Helicobacter pylori vacA Mosaicism and New Primers for vacA Signal Sequence Indigenous to Korea (Helicobacter pylori vacA 대립유전자의 Mosaicism과 Signal Sequence의 한국고유 시발체)

  • Ahn, Yeon-Hwa;Kim, Heung-Ryel;Lee, Ji-Eun;Hwang, Tae-Sook;Choe, Yon-Ho
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.4 no.2
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    • pp.155-160
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    • 2001
  • Purpose: Helicobacter pylori has been known to have diverse vacA allelic types. The purpose of the study was to identify vacA diversity in Korea and design new primers for signal sequence alleles indigenous to Korea. Methods: Fifty antral biopsy specimens, which had been proven to be H. pylori-positive, were examined for vacA status; signal sequence and mid-region. After PCR amplification and DNA sequencing, vacA alleles of Korean H. pylori strains were compared with those from other countries. Results: Among Korean H. pylori strains vacA alleles with all combinations of signal sequence and mid-region were found, with the exception of s1b or s2. vacA genotype s1c/m1 was predominant in Korea. We found that GGGAGCGTTR in s1a and GGGGYTATTG in s1c were the indigenous sequences to Korea and constructed the new Korean specific primers for the vacA signal sequence; VASK-F, VASK-R, S1AK-F, and S1CK-F. Conclusion: This study showed that s1c/m1 is the predominant type of vacA allele in Korea. We designed new primers for the vacA signal sequence.

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Predictive Factors of Turnover Intention in New Nurses (신규간호사의 이직의도에 미치는 영향요인)

  • Lee, Jeong Hwa;Noh, Hye Ran;Park, Ju Young
    • Journal of muscle and joint health
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    • v.27 no.1
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    • pp.50-60
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    • 2020
  • Purpose: This study aimed to identify the effects of preceptor's leadership, preceptor-preceptee exchange relationship, and preceptee's self-leadership on newly hired nurses' turnover intention. Methods: Participants were 175 nurses working at general hospitals in D city. Data were collected from 164 nurses in May 2019 and were analyzed using multiple linear regression with SPSS/WIN 24.0 program. Results: Newly hired nurses' turnover intention was negatively correlated with preceptor's transactional leadership (r=-.23, p=.003), preceptor's emotional leadership (r=-.28, p<.001), preceptor-preceptee exchange relationship (r=-.34, p<.001), and preceptee's self-leadership (r=-.23, p=.004). The work unit (β=.35, p<.001), preceptor-preceptee exchange relationship (β=-.28, p=.002), present work experience (β=.27, p=.043), and marital status (β=-.19, p=.009) were significant predictors of the turnover intention (R2=.26, p<.001). Conclusion: Therefore, in order to reduce the degree of turnover of new nurses, improving the exchange relationship of preceptor-preceptee is required, and balancing between work and life should be considered especially for the married new nurses.

A Numerical Study on Flame Stability with Extended Combustor in Superdetonative Mode Ram Accelerator (초폭굉 모드 램 가속기의 연소실 길이에 따른 화염유지특성에 대한 수치적 연구)

  • Sung, Kun-Min;Jeung, In-Seuck
    • Journal of the Korean Society of Combustion
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    • v.12 no.4
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    • pp.31-38
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    • 2007
  • An numerical study was conducted on superdetonative mode ram accelerator with length extended combustor. The computation condition was based on ISL's RAMAC30 II S225 experiment. For 50% length increased combustor, flame is not sustained. For the case of 60% and 70% increase, flame is successfully sustaind. But detonation wave is oscillating and acceleration is fluctuating. Extention of combustor is helpful for sustaing detonation wave but it may cause unstart.

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Effect of Shading, Light Quality, and Chemical Elicitation on Growth and Bioactive Compound Content of Potentilla kleiniana Wight et Arnott (가락지나물의 생장과 생리활성물질 함량에 미치는 차광, 광질 및 화학적 엘리시테이션)

  • Lee, Jong-Du;Park, Jung-Ae;Park, Byung-Jun;Jeong, Cheol-Seung;Park, So-Young;Pae, Kee-Yoeup
    • Korean Journal of Plant Resources
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    • v.29 no.4
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    • pp.363-375
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    • 2016
  • Potentilla kleiniana is a perennial herb beloning to Rosaceae family. Herein we investigated the effect of light intensity, light quality and chemical elicitor on plant growth and the accumulation of bioactive compounds in P. kleiniana. After 60 days of cultivation under different shading level [0% (200 μmol·m−2·s−1), 35% (95 μmol·m−2·s−1), 55% (65 μmol·m−2·s−1), 75% (40 μmol·m−2·s−1)] in the greenhouse, chlorophyll and carotenoid content were the highest under 35% treatment, however, plant height, leaf number and biomass were the highest under non-shading. As a result of cultivation among strong light condition as a control, florescence and three mixture light sources [red:white:blue (RWB) = 8:1:1, red:blue (RB) = 8:2, red:green:blue (RGB) = 8:1:1] as treatments in plant growth chamber (25 ± 2℃, 185 ± 3 μmol·m−2·s−1), growth, biomass, chlorophyll content low difference between total phenolic compouds and flavonoid content were higher under RWB treatment. DPPH radical elimination ability was the highest under all treatments especially florescence and RGB treatment except control. As a result of treating chemical elicitor [salicylic acid (SA), methyl jasmonate (MeJA)] concentration (0, 50, 100, 200 μM) respectively, plant height, petiole diameter and biomass were higher under non-treatment, MeJA 50 μM. It was investigated that fresh weight and dry weight under MeJA 50 μM treatment were especially a little high. Total phenolic compounds and flavonoid content of SA 50 μM treatment was the highest but DPPH radical elimination ability was significantly the highest under MeJA 200 μM (88.65%) and MeJA 50 μM (87.84%) treatment. Thus, this study suggested that we determined optimal shading and light quality in the greenhouse and plant growth chamber also confirmed bioactive compound content, antioxidant ratio increase according to different chemical elicitation concentration.

A 1.8V 50-MS/s 10-bit 0.18-um CMOS Pipelined ADC without SHA

  • Uh, Ji-Hun;Kim, Won-Myung;Kim, Sang-Hun;Jang, Young-Chan
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.05a
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    • pp.143-146
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    • 2011
  • A 50-MS/s 10-bit pipelined ADC with 1.2Vpp differential input range is proposed in this paper. The designed pipelined ADC consists of eight stage of 1.5bit/stage, one stage of 2bit/stage, digital error correction block, bias & reference driver, and clock generator. 1.5bit/stage is consists of sub-ADC, DAC and gain stage, Specially, a sample-and hold amplifier (SHA) is removed in the designed pipelined ADC to reduce the hardware and power consumption. Also, the proposed bootstrapped switch improves the Linearity of the input analog switch and the dynamic performance of the total ADC. The reference voltage was driven by using the on-chip reference driver without external reference. The proposed pipelined ADC was designed by using a 0.18um 1-poly 5-metal CMOS process with 1.8V supply. The total area including the power decoupling capacitor and power consumption are $0.95mm^2$ and 60mW, respectively. Also, the simulation result shows the ENOB of 9.3-bit at the Nyquist sampling rate.

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Charateristics upon aging period of depanding on distillation method using Saccharomyces cerevisiae 88-4 isolated from traditional Nuruk (전통누룩에서 분리한 효모 88-4로 제조한 술의 증류방법에 따른 숙성기간 중 품질 특성)

  • Lee, Dae-Hyoung;Kang, Heui-Yun;Seo, Jae-Soon;Won, Seon-Yi;Kim, Tae-Wan;Kim, Jae-Ho
    • Korean Journal of Food Science and Technology
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    • v.50 no.4
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    • pp.407-413
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    • 2018
  • The current study reviews the manufacturing characteristics, upon aging, using different distillation methods with Saccharomyces cerevisiae 88-4 isolated from traditional Nuruk. The alcohol content slightly decreased at 180 days of aging period, under the conditions: -60 cmHg, distilling temperature $50^{\circ}C$, head cut 7%, and body cut 30%. The alcohol content decreased by 2.2% at: -50 cmHg, distilling temperature $60^{\circ}C$, head cut 7%, and body cut 30%. From 2-thiobarbituric acid (TBA) analysis, the TBA values were not found to change significantly in most of the alcohols, but increased at conditions of: -60 cmHg, distilling temperature $60^{\circ}C$, head cut 7%, and body cut 50% and -50 cmHg, distilling temperature $50^{\circ}C$, head cut 7%, and body cut 50%, when initial alcohol levels were low. According to n-propanol, iso-butanol, and isoamyl alcohol analysis, with increasing aging period, the n-propanol and isoamyl alcohol content did not change, although that of iso-butanol decreased. The flavor components showed different results based on the aging period. Results of sensory evaluation showed no significant difference based on aging. The sensory evaluation test was continued for 180 days at a condition of: -60 cmHg, distilling temperature $60^{\circ}C$, and body cut 50%, which had the best overall evaluation ($5.8{\pm}0.9$).

Axisymmetric Simulation of Nonpremixed Counterflow Flames - Effects of Fuel Concentration on Flame Structure - (비예혼합 대향류 화염의 축대칭 모사 - 연료농도가 화염구조에 미치는 영향 -)

  • Park Woe-Chul
    • Journal of the Korean Institute of Gas
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    • v.7 no.3 s.20
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    • pp.44-50
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    • 2003
  • The axisymmetric methane-air counterflow flame was simulated to investigate changes in the flame structure due to the fuel concentration and to evaluate the numerical method. The global strain rates $a_g=20,\;60,\;90\;s^{-1}$ and the mole fractions of methane $x_m=20,\;50,\;80\%$ in the fuel stream were taken to be numerical parameters. The axisymmetric simulation was conducted by using the Fire Dynamics Simulator (FDS) which employed a mixture fraction combustion model, and the results were compared with those of OPPDIF, which is an one-dimensional flamelet code and includes detail chemical reactions. In all the cases tested, there was good agreement in the temperature and axial velocity profiles between the axisymmetric and one-dimensional simulations. It was shown that the flame thickness and peak flame temperature increase and the flame radius decreases as the fuel concentration increases.

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Protease Properties of Protease-Producing Bacteria Isolated from the Digestive Tract of Octopus vulgaris (Octopus vulgaris의 장관으로부터 분리한 단백질 분해효소 생성 균주와 생성된 효소의 특성)

  • Liu, Qing;Ren, Pei;Piao, Meizi;Yang, Ji-Young
    • Journal of Life Science
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    • v.23 no.12
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    • pp.1486-1494
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    • 2013
  • A high protease-producing strain was isolated and identified from the digestive tract of octopus vulgaris by detecting a hydrolysis circle of protease and its activity. The strain was identified by morphology observation, biochemical experiments, and 16S rRNA sequence analysis. The protease obtained from the strain was purified by a three-step process involving ammonium sulfate precipitation, carboxy methyl-cellulose (CM-52) cation-exchange chromatography, and DEAE-Sephadex A50 anion-exchange chromatography. The properties of protease were characterized as well. The strain Bacillus sp. QDV-3, which produced the highest activity of protease, was isolated. On the basis of the phenotypic and biochemical characterization and 16S rRNA gene-sequencing studies, the isolate was identified as follows: domain: Bacteria; phylum: Firmicutes; class: Bacilli; order: Bacillales; family: Bacillaceae; and genus: Bacillus. The isolate was shown to have a 99.2% similarity with Bacillus flexus. A high active protease designated as QDV-E, with a molecular weight of 61.6 kDa, was obtained. The enzyme was found to be active in the pH range of 9.0-9.5 and its optimum temperature was $40^{\circ}C$. The protease activity retained more than 96% at the temperature of $50^{\circ}C$ for 60 min. Phenylmethylsulfonyl fluoride (PMSF) inhibited the enzyme activity, thus confirming that this protease isolated from Bacillus sp. QDV-3 is an alkaline serine protease. Metal ions, $Mn^{2+}$ and $Mg^{2+}$, were determined to enhance the protease activity, whereas $Ba^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ were found to inactivate the enzyme.

Study on Poly(triazine bissulfide)s Derivatives being the Synthesized Optical Plastic Material (합성한 광학플라스틱 재료인 Poly(triazine bissulfide)s 유도체에 대한 연구)

  • Lee, Y.H.;Lee, D.H.;Kim, J.J.;Ha, T.W.;Cha, J.W.
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.2
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    • pp.481-489
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    • 2004
  • Poly(triazine bissulfide)s were synthesized from 6-dibutylamino-1,3,5-triazine-2,4-dithiol with bis(4-chloro-3-nitrophenyl)sulfone in the presence of the phase transfer catalyst, and from m-dibromide xylene and p-dibromide xylene with 6-dibutylamino-1,3,5-triazine-2,4-dithiol in the presence of cetyltrimethyl ammonium bromide at $70^{\circ}C$ for 24h. We could acquire the good results about solubility, thermal property, and molecular weight to make cast film. These results are important as base for the synthesis of functionalization polymer material being optical plastic material. The maximum algebra viscosity was 0.57~1.40dl/g at the temperature more than $50{\sim}60^{\circ}C$.

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