• 대한수의학회지
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• 제35권1호
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• pp.39-44
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• 1995

도롱뇽(Hynobius leechii)의 위장관 내분비세포를 면역조직화학적으로 관찰하였던 바, BPP 면역반응세포는 유문부에서 중등도, 십이지장과 소장에서 소수로 관찰되었다. 중등도의 somatostatin 면역반응세포는 유문부에 국한되어 출현하였다. 5-HT 면역반응세포는 전장관에 걸쳐 출현하였으나 유문부에서 다수 그리고 직장에서 극소수로 분포하였다. 특히 BPP와 5-HT면역반응세포는 도롱뇽에서 최초로 동정하였다.

• 대한수의학회지
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• 제36권3호
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• pp.513-519
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• 1996

The development of calcitonin, serotonin(5-HT) and bovine chromogranin (BCG)-immunoreactive cells was investigated in the thyroid glands of Korean native goat from fetus to adult, using an immunohistochemical method. Moderate number of calcitonin-immunoreactive cells were present in the thyroid glands from 1 month to 6 months after birth, whereas a few in the adult. They were not present in the thyroid glands of fetus and 1 day after birth. A number of 5-HT- and BCG-immunoreactive cells were present in the thyroid glands of Korean native goat from fetus to adult. They were increased in number with age. BCG-immunoreactive cells were weakly reacted in fetus and 1 day after birth, while these cell were strongly stained from 1 month to adult. These findings suggest that calcitonin-, 5-HT- and BCG-immunoreactive cells were present in the thyroid gland of Korean native goat.

• 한국식품영양학회지
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• 제20권3호
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• pp.282-288
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• 2007

The quality characteristics of cookies between fresh shredded garlic(FSG) and heat-treated($150^{\circ}C$, 5 min) shredded garlic (HT-SG) were comparatively analyzed based on different content additions. The levels investigated were 0, 0.5, 1, 2, 4 and 6% for both shredded garlics. Dough pH was lower in the FSG-added cookies than in the HT-SG-added cookies. The spread factor decreased with the addition of shredded garlic compared to the control($10.63{\pm}0.57$), but differences were insignificant within each group. Cookies hardness was highest at 2% garlic content, and it was higher for HT-SG than FSG. Compared to the measured L-value of the control, the L-values of the test samples decreased with increasing garlic contents. With increasing garlic contents, the a-value increased, but the b-value remained mostly unchanged, with less significant difference compared to the control. The color of the HT-SG group tended to be brown as the garlic content increased. The degree of browning was more pronounced for HT-SG than FSG. Surface cracking of the cookies tended to be higher in the FSG samples than in HT-SG. Compared to the control, garlic taste and flavor increased remarkably with increasing garlic content, and they were stronger for the FSG cookies. The overall acceptability of HT-SG was relatively high, and it was highest for the HT-SG cookies with a 0.5~2% garlic contents than for the control cookies.

• The Korean Journal of Pain
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• 제26권2호
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• pp.125-129
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• 2013

Background: 5-hydroxytryptamine 3 (5-HT3) receptors have been known to be associated with the modulation of nociceptive transmission. However, it is uncertain whether 5-HT3 plays a role in the antinociceptive or pronociceptive pathway for incisional pain. In this study, we evaluated the effects of palonosetron, a 5-HT3 receptor antagonist, on incisional pain in rats when administered intrathecally or intraplantarly. Methods: An intrathecal catheter was implanted through the cisterna magna and placed in the intrathecal space of rats. An incision in the plantaris muscle of the right hind paw was done under anesthesia with sevoflurane. Withdrawal thresholds were evaluated with the von Frey filament after 2 hours. Palonosetron (0.5 and 0.1 ${\mu}g$ intrathecally; 0.5 ${\mu}g$ intraplantarly) was administered and the thresholds were observed for 4 hours. Results: Mechanical hypersensitivity developed after the incision. Intrathecal palonosetron (0.5 ${\mu}g$ and 0.1 ${\mu}g$) did not alter the paw withdrawal threshold. Intraplantar palonosetron (0.5 ${\mu}g$) also did not change the paw withdrawal threshold. Conclusions: Intrathecal and intraplantar palonosetron (0.5 ${\mu}g$) had no effect on modulating the mechanical hypersensitivity in the incisional pain model of rats.

• Journal of the Korean Wood Science and Technology
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• 제48권1호
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• pp.76-85
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• 2020

This study was conducted to analyze the application of boron compounds, methyl methacrylate (MMA), and heat treatment (HT) on changes in the density, moisture content, and flexural properties of samama (Anthocephalus macrophyllus) wood. Samama wood was impregnated with borax (BX) and boric acid (BA) using a pressure method at 5 atm for 4 h. Afterwards, the wood was impregnated with MMA at the same pressure and duration. Finally, the samama wood was given HT at 90 ℃ and 180 ℃. The results indicate that there was a weight gain of 93.4% in the wood impregnated using BA and MMA monomer and HT at 90 ℃. Consequently, the wood's density increased by 82.3%. Increased MOE and MOR percentages of 32.2% and 29.4%, respectively, were also found. HT at 180 ℃ degraded the wood components and MMA, and consequently, the density, MOE, and MOR also decreased. The wood impregnated by BX, BA, and MMA, and subjected to HT also had decreased moisture content (MC). This research recommends that the application of boron (BX, BA) should be combined with an MMA monomer and HT at 90 ℃ as an alternative method to improve samama wood quality. If darker color is preferable, HT should be conducted at 180 ℃.

• 생물정신의학
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• 제11권1호
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• pp.54-60
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• 2004

Objectives:Tardive dyskinesia(TD) is a serious side effect associated with long-term antipsychotic treatments. Some candidate genetic polymorphisms were reported to be associated with TD and possible involvement of serotonergic receptors in the pathophysiology of TD has been suggested. In the present study, we investigated the association between $5-HT_6$ receptor gene polymorphism and TD with schizophrenia. Methods:To investigate the relationship between $5-HT_6$ receptor gene polymorphism and TD, 60 patients with TD were compared with 60 patients without TD. The 267C/T allele of $5-HT_6$ receptor gene was genotyped by means of polymerase chain reaction method. TD was evaluated using the Abnormal Involuntary Movement Scale(AIMS). Results:The patients with the three 267C/T genotype showed no significant differences in age, gender, and duration of illness. No significant difference in genotype frequencies was observed between schizophrenic patients with and without TD. In addition, there was no difference in allele frequencies. Further analysis with an measure of AIMS scores showed that these scores were not significantly influenced by the $5-HT_6$ receptor gene polymorphism. Conclusion:These results suggest that 267C/T polymorphism of $5-HT_6$ receptor gene is not significantly associated with susceptibility to TD in schizophrenia.

• 대한본초학회지
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• 제25권1호
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• pp.75-81
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• 2010

Objectives : It was investigated new herbal prescription HT005 supported by traditional Korean medicine has a activity on the longitudinal bone growth of rats. HT005 were consisted of the root of Angelica sinensis, the fruit of Alpinia oxyphylla, the root of Astragalus membranaceus, the stem of Eleutherococcus senticosus, and the root of Dioscorea japonica, and Poria cocos. Method : To investigate the effect on longitudinal bone growth in adolescent male Sprague-Dawley rats, the longitudinal length of growth plate was measured directly by the tetracycline fluorescent labeling method and chondrocyte staining method. HT005 administered orally for four days, and the tetracycline was injected twice on the growth plate of the animals for fluorescent dying. The rate of longitudinal bone growth was measured the length between the tetracycline bands which fixed on the 3rd day and 5th day after injection. Cresyl violet was also used to stain the chondrocytes in the growth plate. The length of growth plate after administration was compared. Expression of IGF-1 and BMP-2 in the growth plate was investigated by immunohistochemistry. Results : HT005 showed a significant longitudinal bone growth which was $301.0{\pm}6.1\;{\mu}m/day$ at the dose of 100 mg/kg and $283.8{\pm}1.25\;{\mu}m/day$ (p < 0.001)at the dose of 10 mg/kg of HT005, compared to control group by the tetracycline fluorescent labeling method. HT005 showed a significant chondrocyte length on growth plate which was measured $797.19{\pm}3.31\;{\mu}m$ (p < 0.001) at the dose of 100 mg/kg and $720.14{\pm}2.19$ (p < 0.001) ${\mu}m$ at the dose of 10 mg/kg compared to the control group by cresyl-violet staining method. Both the number and intensity of BMP-2 and IGF-1 positive cells were increased in the hypertrophic zone of growth plate. There was a significant correlation between BMP-2 and IGF-1 expression and heights of chondrocytes in growth plate. Conclusions : Treatment of HT005 on Sprague-Dawley rats markedly increased the longitudinal bone growth. Therefore, HT005 may be an alternative herbal source to growth hormone as it promotes bone growth in children afflicted by growth retardation.

• Bulletin of the Korean Chemical Society
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• 제24권10호
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• pp.1512-1514
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• 2003

• 한국식품영양과학회지
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• 제46권7호
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• pp.886-890
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• 2017

상황버섯균사체 쌀의 뇌세포 보호 효과를 분석하여 상황버섯균사체 쌀에 대한 기초자료를 제공하고, 이를 이용하여 뇌 질환의 예방과 관련된 기능성 식품으로의 개발가능성을 찾고자 하였다. 상황버섯균사체 쌀 추출물을 처리하여 마우스 해마세포주 HT22의 세포보호 효과를 알아보기 위해 MTS assay를 이용한 세포생존율과 western blot을 이용한 세포사멸 단백질의 발현을 관찰하였다. 상황버섯균사체 쌀 추출물의 단일 독성을 측정한 결과 상황버섯균사체 쌀 추출물 처리군은 102.68% 이상의 생존율을 보여 신경세포의 사멸에 영향을 주지 않았다. 또한, 상황버섯균사체 쌀 추출물과 $H_2O_2$를 함께 처리하였을 때 $H_2O_2$로 자극된 세포는 63.80%의 세포생존율을 나타내었고, 상황버섯균사체 쌀 추출물과 $H_2O_2$가 함께 처리된 세포는 76.85%, 92.46%, 95.57%로 농도 의존적으로 세포생존율이 증가하였다. HT22 cell에 $H_2O_2$와 상황버섯균사체 쌀 추출물을 처리하여 apoptosis 유도 단백질을 측정한 결과, pro-apoptotic protein인 Bax는 발현이 억제되었고, anti-apoptotic protein인 Bcl-2는 발현이 증가함을 확인하였다. 또한, caspase-3와 PARP의 발현을 억제하여 상황버섯균사체 쌀 추출물이 $H_2O_2$로 유도되는 apoptosis를 억제하는 것을 확인할 수 있었다.

• 한국의학물리학회지:의학물리
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• 제25권2호
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• pp.89-94
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• 2014

국소 진행성 직장암 환자에서 수술 전 항암방사선동시요법으로 치료시 추가적인 고주파 온열치료 유무가 수술 후 부작용에 미치는 영향을 분석하였다. 1996년부터 2007년 사이, 본원에서 수술 전 항암방사선동시요법과 근치적 수술을 시행한 환자 205명을 대상으로 급, 만성부작용을 분석하였다. 총 방사선치료선량은 39.6 Gy에서 45 Gy였고 1회 내지 2회의 항암약물치료(5-fluorouracil, leucovorin)를 동시에 시행하였다. 88명의 환자가 주 2회, 8-MHz 고주파 온열치료기를 이용한 국소 온열치료를 시행하였다. 외과적 수술은 수술 전 치료 완결 후 4~6주 경과하여 시행하였다. 환자군의 나이 중앙값은 59세(18세~83세)이고 추적관찰기간 중앙값은 61개월(2개월~191개월)이었다. 전체 환자에서 5년 전체생존율과 무합병증 생존율은 77.4%와 73.7%였다. 각각의 조기 누출, 지연 누출, 연결부 협착, 누공, 소장폐쇄의 발생빈도는 1.0%, 2.9%, 1.5%, 5.9%, 그리고 17.1%였다. 온열치료는 모든 종류의 부작용을 증가시키지 않았다. 온열치료를 실시하지 않은 군과 온열치료군 간의 5년 무합병증 생존율은 71.8%와 76.3%였다(p=0.293). 온열치료는 수술전 항암방사선동시요법 후 근치적 수술을 시행하는 국소 진행 직장암 환자의 수술후 부작용을 증가시키지 않는다.