• Title/Summary/Keyword: 4W

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Application of 16S rDNA PCR-RFLP Analysis for the Rapid Identification of Weissella Species (Weissella 속 유산균의 빠른 동정을 위한 16S rDNA PCR-RFLP 분석법의 적용)

  • Lee, Myeong-Jae;Cho, Kyeung-Hee;Lee, Jong-Hoon
    • Microbiology and Biotechnology Letters
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    • v.38 no.4
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    • pp.455-460
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    • 2010
  • A polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis was applied to detect and identify ten Weissella spp. frequently found in kimchi. The previously reported genus-specific primers designed from 16S rDNA sequences of Weissella spp. were adopted but PCR was performed at the increased annealing temperature by $4^{\circ}C$. The sizes of amplified PCR products and restricted fragments produced by AluI, MseI, and BceAI endonucleases were well correspond with the expected sizes. W. kandleri, W. koreensis, W. confusa, W. minor, W. viridescens, W. cibaria, and W. soli were distinguished by AluI and MseI and W. hellenica and W. paramesenteroides were identified by BceAI. W. thailandensis was distinguished when restriction pattern of other species was compared but identified by the single use of MspI.

The Optomotor Response of Crusian Carp and Goldfish in Accordance with the Colors of the Wall and the Stripes of the Visual Screen (시각막의 바탕과 줄무늬의 색상에 따른 붕어와 금붕어의 시각운동 반응)

  • 장충식
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.20 no.1
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    • pp.17-22
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    • 1984
  • The author carried out an experiment to find on the optomotor response of crusian carp, Carassius carassius and goldfish, Carassius auratus in accordance with the colors of the wall and the stripes labeled on the revolving visual screen. The results are as follows; 1. The optomotor response rate of crusian carp was 80% or more in case of B-Y, R-Bl and W-Bl pair of colors, and 50% or less in case of Bl-B, B-Bl, R-W, R-Y, W-Y and Y-W pair. 2. The optomotor response rate of goldfish was 80% or more in all except B-Bl, W-R and W-Y pair. 3. Mean round number of crusian carp was 7.5-9.5 revolutions in case of W-Bl, Y-Bl, W-B and Y-B pair, and 1.8-3.9 revolutions in case of Bl-B, Bl-Y, B-Bl, B-R, R-All and Y-W pair for 3 minutes. 4. Mean rounded number of goldfish was only 4.1-4.9 revolutions in case of W-Bl, W-B and Y-B pair for 3 minutes.

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Studies on the Nutritional Components of Dandelion(Taraxacum officinale) (민들레의 영양성분에 관한 연구)

  • 신승렬
    • Food Science and Preservation
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    • v.6 no.4
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    • pp.495-499
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    • 1999
  • The free sugars in leaf and root of dandelion were composed of sucrose, glucose and fructose. The contents of total free sugars was higher in root than those in leaf. The oxalic acid, citric acid and malic acid contents of leaf was 45.4, 3.6, 2.7mg/100g-f.w., respectively. And the oxalic acid, citric acid and malic acid contents of root was 34.6, 2.1, 1.6mg/100g-f.w., respectively. Total free organic acid content of leaf was higher than that of root. The major free amino acids of dandelion were aspartic acid, serine, asparagine, glutamic acid, glycine, valine, isoleucine and content of glutamic acid was highest in free amino acids. The contents of vitamin A in leaf and root of dandelion was 135.4 and 34.1$\mu\textrm{g}$/100g-f.w., respectively. The contents of vitamin C in leaf and root of dandelion was 67.4 and 4.6 mg/100g-f.w., respectively.

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Effect of Deposition Time and Pressure on Properties of Selective CVD-W by $SiH_4$ Reduction ($SiH_4$ 환원에 의한 Selective CVD-W막 특성에 대한 증착시간과 압력의 효과)

  • Lee, Chong-Mu;Lee, Kang-Uk;Park, Sun-Hoo
    • Korean Journal of Materials Research
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    • v.1 no.4
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    • pp.177-183
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    • 1991
  • Change of the properties of selective CVD-W by $SiH_4$ reduction with the variation of deposition time and pressure has been investigated. The lime required for covering the who)to Si substrate by tungsten at $300^{\circ}C$ under the pressure of 100mtorr is approximately 30 seconds. The film thickness tends to increase linearly in the early stage of deposition process and parabolically later, sheer resistance of the film tends to decrease rapidly initially, and slowly later with deposition time. Tungsten grain size does not change much, but grain boundary becomes hazy in the pressure range of 50-300mtorr. Also no ${\beta}-W$ but only ${\alpha}-W$ was found in this pressure range. The deposition rate and electrical resistivity of tungsten tend to increase wish increasing pressure. The results of AES analysis show that pressure does not much affect Si/W ratio of the tungsten film and silicidation at the W/Si interface.

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Characteristics of AlW thin film for TFT-FCD bus line

  • Kim, Dong-Sik;Yi, Chong-Ho;Chung, Kwan-Soo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.58-58
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    • 2000
  • Recently low resistance of bus line is required for large screen size RFT-CLD panels. As a result, lower resistance Al-alloy is currently reviewed extensively. The resistivity is required smaller than 10$\mu$$\Omega$cm and high resistance of chemical attack is required. In this paper, Al-W thin film were deposited on glass substrates by D.C magnetron sputtering system under various condition for high chemical resistance. Its properties were characterized by SEM, AFM, XRD, 4-point-probe, and cyclic voltammertry. The optimal condition of Al-W was 10$0^{\circ}C$, 100W, 0.4Pa, 23sccm(Ar) and 35$0^{\circ}C$, 20min. annealing. At that condition the resistivity of Al-W(3 wt.%) was about 11$\mu$$\Omega$cm. And when wt.% of W in Al-W alloy was higher than about 4%, Al-W alloy thin film has high chemical resistance.

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Synthesis of TAME, ETBE, and MTBE Using Heteropolyacid Catalyst (헤테로폴리산 촉매를 이용한 TAME, ETBE 및 MTBE 합성반응의 연구)

  • Park, Jin-Hwa;Yi, Yong-Woo
    • Applied Chemistry for Engineering
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    • v.8 no.4
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    • pp.582-588
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    • 1997
  • Synthetic reaction of TAME, ETBE, and MTBE compounds used largely for gasoline octane number enhancer to prevent air pollution was investigated using heteropolyacid catalyst in a fixed bed flow reactor. In the synthetic reaction of TAME, ETBE and MTBE, after hetero atom being replaced with poly atom, the activity of the catalyst, $H_4SiW_{12}O_{40}$ with coordinated bond with W and an hetero atom of Si was the highest among the catalysts tested. Also the activity depended upon the metals replaced which are related to the catalyst acidity. $FeHPW_{12}O_{40}$ and $K_3PM_{o12}O_{40}$ catalysts showed high activity in TAME synthesis, while they were not effective in ETBE and MTBE synthesis. In this study catalysts showing high activity were selected and mixed with equal weight combination of $H_4SiW_{12}O_{40}$ and $Sr_2SiW_{12}O_{40}$ ;$H_4SiW_{12}O_{40}$ and $NaH_2PW_{12}O_{40}$ ; $Fe_{1.5}PW_{12}O_{40}$ and $Mg_2SiW_{12}O_{40}$ ; $Mg_2SiW_{12}O_{40}$ and $Ba_2SiW_{12}O_{40}$. The mixed heteropolyacid catalysts showed higher TBA conversion rate and better selectivity of ETBE and MTBE than the single catalysts.

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Effect of Flywheel Weight on Engine Performance for the Small Diesel Engine (Flywheel의 중량(重量)이 소형(小型) 디젤기관(機關)의 성능(性能)에 미치는 영향(影響))

  • Jung, Hae Kook;Kim, Sung Rai;Myung, Byung Soo
    • Korean Journal of Agricultural Science
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    • v.15 no.2
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    • pp.143-152
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    • 1988
  • This study was conducted to obtain basic data which affected engine performance of the power tiller being widely used in the rural area. Among the various factors affected engine performance, only flywheel weight was considered as the major factor in this study. Fuel consumption ratio, motoring loss, torque, vibration and mechanical efficiency of the engine tested were measured and analyzed on the four levels of flywheel weight (32.2, 29.7, 26.4, 24.2 kg). The results obtained were as follows: 1. The maximum output of 6 and 7.5 kW engine was 7.43 kW and 7.85 kW respectively. When flywheel weight was reduced from 32.2 kg to 24.2 kg, output power of the engine was increased 0.27 kW in 6 kW engine and increased 0.39 kW in 7.5 kW engine. 2. The fuel consumption ratio was decreased from 300.8 to 296.8 g/kW-hr in 6 kW engine and decreased from 313.6 to 312.8 g/kW-hr in 7.5 kW engine when the flywheel weight was reduced from 32.2 kg to 24.2 kg. 3. The mechanical efficiencies of the engine was increased from 76.1 to 76.8% in 6 kW engine and increased from 76.7 to 77.0% in 7.5 kW engine when the flywheel weight was reduced from 32.2 kg to 24.2 kg. 4. When the flywheel weight was reduced from 32.2 kg to 24.2 kg, a tendency of a little decrease of vibration at X- and Z-axis in 6 kW engine and of a little increase of vibration at Y-axis in 6 kW engine and all directions in 7.5 kW engine was observed. 5. Motoring losses was decreased from 2.33 to l.76 kW in 6 kW engine and decreased from 2.46 to 1.84 kW in 7.5 kW engine when the flywheel weight was reduced from 32.2 kg to 24.2 kg. From the above results and the flywheel weight calculated theoretically, it was recommendable that the flywheel weight should be reduced about 7 kg in 6 kW engine and about 10 kg in 7.5 kW engine, respectively.

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SATELLITE ATTITUDE SENSING MODEL AND THEIR S/W DEVELOPMENT (인공위성 자세감지 모델과 그 S/W 개발)

  • 김영신;안웅영;김천휘
    • Journal of Astronomy and Space Sciences
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    • v.16 no.1
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    • pp.69-78
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    • 1999
  • We have developed an attitude sensing S/W system, one of modules of Mission Analysis System(MAS), which simulates attitude sensing data as almost the same as the real sensor of a satellite in orbit. When attitude elements($alpha,delta$) of a satellite and positions of Earth, Moon, and Sun are given, the S/W system calculates look angles and dihedral angles of each celestial bodies relative to the rotations axis of the satellite. It consists of two sub-modules : One is ephemeris service module which consider the perturbations of four planets(Venus, Mars, Jupiter, Saturn) for positions of Sun and Moon and 4 $\times$4 earth gravitational potential terms for a satellite's position. The other is attitude simulation module which generates attitude sensing data. Varying the rotational axis of a satellite and it's orbital elements, we simulated the generating attitude sensing data with this S/W system and discussed their results.

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Effects of Security Lighting Lamps on Discomfort Glare to Indoor Occupants

  • Lee, Yoon Jeong;Kim, Jeong Tai
    • KIEAE Journal
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    • v.15 no.4
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    • pp.21-28
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    • 2015
  • Purpose: Although security lights are used to secure pedestrian visibility and safety at nighttime, they can generate light trespass in the neighboring residential space. To prevent this, standards for acceptance limits on vertical illuminance and light pollution by the windows of residential buildings are presented. Method: This study thus representatively selected three types of lamps and, through an evaluation and analysis of the physical and subjective discomfort glare per lamp, proposed a discomfort glare index for each lamp. The evaluation and analysis according to the lamps were conducted through experiments. The variables were the security lights' lamps (NH 100W, MH 70W, LED 50W), installation angles ($0^{\circ}$, $20^{\circ}$), and installation distances (3m, 5m, 7m, 9m). Result: According to the results of the discomfort glare evaluation depending on the angles and distances of the security lights, the following minimum standards are proposed: for NH 100W, a discomfort glare index of 30 and an installation distance of 4m; for MH 70W, a discomfort glare index of 32 and an installation distance of 4m; and, for LED 50W, a discomfort glare index of 31 and an installation distance of 6m, respectively. In addition, this paper recommends the use of MH 70W, when the road width is 4m-6m, and LED 50W, when the road width is over 6m, respectively.