• The Korean Journal of Zoology
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• v.38 no.3
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• pp.340-347
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• 1995

We have used two kinds of P element constructs, Pc[(ry+)B] and p[(ry+)$\Delta$SX9], for genetic transformation by microinjection of D. melanogaster. Pc[(ry+)B] construct carrying the rosy gene within an autonomous P element was injected into a true M strain caring the ry506. mutation. The source of transposase for microinjection and transformation was provided by a P element helper plasmid designated p-$\Delta$2-3hs$\pi$, which was co-injected with nonautonomous P[(ry+)$\Delta$SX9] construct into same ry506 M strains. A dechorination method was adopted and 35 independent transformed lines were obtained froin 1143 G0 Injected (35/1143). About 20% of the injected embryos eclosed as adults. Among G0 eclosed flies, approximately 40% exhibited eye color that was similar to wild-type (ry+), but about 60% of fertile G0 transformed lines appeared to have no G1 transformants. Therefore it is unlikely that G0 expression requires integration of the rosy transposon into chromosomes. Pc[(ry+)B] and P[(ry+)$\Delta$SX9] constructs were found to be nearly same in the frequency of element-mediated transformation. On the basis of these results, nonautonomous P elements constructs could he used as same effective vectors in P element-mediated transformation for introducing and fixing genes in insect populations.

• Journal of Gastric Cancer
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• v.21 no.4
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• pp.325-334
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• 2021

Purpose: Although dumping symptoms are thought to involve postprandial glycemic changes, postprandial glycemic variability without dumping symptoms remains poorly understood due to the lack of a method that allows the easy and continuous measurement of blood glucose levels. Materials and Methods: Patients having undergone distal gastrectomy with Billroth-I (DG-BI) or Roux-en-Y reconstruction (DG-RY), total gastrectomy with RY (TG-RY) and pylorus preserving gastrectomy (PPG) for gastric cancer 3 months to 3 years prior, diagnosed as pathological stage I or II, were prospectively enrolled from March 2018 to January 2020. The interstitial tissue glycemic levels were measured every 15 min, up to 14 days by continuous glucose monitoring. Moreover, using a diary recording the diet and symptoms, asymptomatic glucose profiles without sugar supplementation within 3 h postprandially were compared among the four procedures. Results: A total of 40 patients were enrolled, 10 patients for each of the four procedures. There were 47 glucose profiles with DG-BI, 46 profiles with DG-RY, 38 profiles with TG-RY, and 46 profiles with PPG. PPG showed the slowest increase with a subsequent gradual decrease in glucose fluctuations, without hyperglycemia or hypoglycemia, among the four procedures. In contrast, TG-RY and DG-RY showed spike-like glycemic variability, sharp rises during meals, and rapid drops. The glucose profiles of DG-BI were milder than those of RY. Conclusions: The asymptomatic glycemic changes after meals differ among the types of surgical procedures for gastric cancer. Given the mild glycemic fluctuations in PPG and the glucose spikes in TG-RY and DG-RY, pylorus preservation and physiological reconstruction without changes in food pathways may optimize postprandial glucose profiles after gastrectomy.

• Journal of Dental Rehabilitation and Applied Science
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• v.40 no.3
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• pp.149-158
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• 2024

Purpose: This study aims to investigate the effects of provisional restoration fabrication methods and surface polishing on surface roughness and microbial adhesion through in vitro experiments. Materials and Methods: 120 cylindrical provisional restoration resin blocks (10 × 10 × 2.5 mm) were manufactured according to four fabrication methods, and 30 specimens were assigned to each group. Afterwards, they were divided into non-polishing group, #400 grit SiC polishing group, and #800 grit SiC polishing group and polished to a 10 × 10 × 2 mm specimen size (n = 10). The surface roughness Ra and Ry of the specimen was measured using a Surface Roughness Tester. Three specimens were extracted from each group and were coated with artificial saliva, and then Streptococcus mutans were cultured on the specimens at 37℃ for 4 hours. The cultured specimens were fixed to fixatives and photographed using a scanning electron microscope. For statistical analysis, the two way of ANOVA was performed for surface roughness Ra and Ry, respectively, and the surface roughness was tested post-mortem with a Scheffe test. Results: The fabrication method and the degree of surface polishing of the provisional restorations had a significant effect on both surface roughness Ra and Ry, and had an interaction effect. There was no significant difference in Ra and Ry values in all polishing groups in DLP and LCD groups. Conclusion: The fabrication method and surface polishing of the provisional restoration had a significant effect on surface roughness and showed different adhesion patterns for S. mutans adhesion.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.343-348
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• 2008

To investigate the effects of incorporation of green manures (GM) into a sandy loam soil on growth, yield, and nutrient uptake of tomato (Lycoperiscon esculentum Mill.) and nutrient balances (input minus offtake of nutrients), five tomato production systems were compared under the condition of plastic film house: 1) a no input system (no additional amendment or inputs, 0-To-0-To); 2) a conventional system (application of N-P-K chemical fertilizers, Cf-To-Cf-To); 3) a leguminous GM-containing system (hairy vetch-tomato-soybean-tomato, Hv-To-Sb-To); 4) a graminaceous GM-containing system (rye-tomato-sudan grass-tomato, Ry-To-Sd-To); and 5) system mixed with leguminous and graminaceous GMs (rye-tomatosoybean- tomato, Ry-To-Sb-To). Here, hairy vetch and rye were cultivated as winter cover crops during late $Dec{\sim}late$ Feb and soybean and sudan grass were cultivated as summer cover crops during late $Jun{\sim}mid$ Aug. All of them cut before tomato planting and then incorporated into soil. Biomass of GMs was greater in summer season than that of winter season. Nitrogen amount fixed by a leguminous plants was about $126\;kg\;ha^{-1}$ per a cropping season, corresponding to 60% N level needed for tomato production, which was comparable to 50 and $96\;kg\;ha^{-1}$ fixed by rye and sudan grass. As a result, tomato yield of Hv-To-Sb-To system (legume GM treatment) was similar to Cf-To-Cf-To (conventional), but that in Ry-To-Sd-To system (graminaceous GM treatment) was not attained to a half level of conventional treatment. Nutrient budgets for N, P and K on the conventional farm were balanced or somewhat positive exception for minus-balanced K. Ry-To-Sd-To system showed a positive N, P and K budgets due to the depressed growth of tomato which is caused by high C/N ratio and low N-fixing capacity of the GMs. Inversely, those of Hv-To-Sb-To system were negative in all of N, P and K budgets because of increased growth and yield of tomato with high nitrogen-supplying capacity as well as low C/N ratio of leguminous GM. In conclusion, although conventional cultivation has an advantage in relation to N, P and K nutrient budgets rather than GM-incorporated systems, a leguminous GMs could be recommended as nitrogen reservoir and soil amendment because the yield of tomato between use of leguminous GM and conventional cultivation was not only significantly difference, but also GMs commonly reduce nutrient loss and improve microbial communities.

• International Journal of Oral Biology
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• v.40 no.4
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• pp.211-216
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• 2015

Nitric Oxide (NO) is an important signaling molecule in the nociceptive process. Our previous study suggested that high concentrations of sodium nitroprusside (SNP), a NO donor, induce a membrane hyperpolarization and outward current through large conductances calcium-activated potassium ($BK_{ca}$) channels in substantia gelatinosa (SG) neurons. In this study, patch clamp recording in spinal slices was used to investigate the sources of $Ca^{2+}$ that induces $Ca^{2+}$-activated potassium currents. Application of SNP induced a membrane hyperpolarization, which was significantly inhibited by hemoglobin and 2-(4-carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (c-PTIO), NO scavengers. SNP-induced hyperpolarization was decreased in the presence of charybdotoxin, a selective $BK_{Ca}$ channel blocker. In addition, SNP-induced response was significantly blocked by pretreatment of thapsigargin which can remove $Ca^{2+}$ in endoplasmic reticulum, and decreased by pretreatment of dentrolene, a ryanodine receptors (RyR) blocker. These data suggested that NO induces a membrane hyperpolarization through $BK_{ca}$ channels, which are activated by intracellular $Ca^{2+}$ increase via activation of RyR of $Ca^{2+}$ stores.

• The Korean Journal of Pharmacology
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• v.31 no.2
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• pp.207-217
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• 1995

To investigate the functional and immunological properties of the Ca-release channel in the sarcoplasmic reticulum(SR) of the eel skeletal muscle, $[^3H]ryanodine$ binding, SDS gel electrophoresis, $^{45}Ca\;release$ studies, and immunoblot assay were carried out in the SR of the eel skeletal muscle. Maximal binding sites(Bmax) and $K_D$ values of $[^3H]ryanodine$ for Ca-release channel of the SR of the eel skeletal muscle were $19.44{\pm}1.40\;pmole/mg$ protein and $15.55{\pm}1.69\;nM$, respectively. $[^3H]Ryanodine$ binding to RyR was increased by calcium and AMP. The SR of the eel skeletal muscle has two high molecular weight bands on the SDS PAGE. The mobility of upper band was more slower than the single band of the rabbit skeletal muscle, and that of the lower band was similar with the single band of canine cardiac muscle. Vesicular $^{45}Ca-release$ was activated by calcium. Ca-induced $^{45}Ca-release$ was significantly inhibited by $MgCl_2(2\;mM)$, ruthenium red$(10\;{/mu}M)$ or tetracaine(1 mM), but not by high concentration of calcium itself. AMP-induced $^{45}Ca-release$ was slightly occurred only in the absence of calcium, it was not inhibited by $MgCl_2$ or ruthenium red. Caffeine also increased $^{45}Ca-release$ from the SR vesicles, but it was not affected by $MgCl_2$ or ruthenium red. Polyclonal Ab against rat skeletal muscle RyR is reacted with that of rabbit, but not reacted with that of the eel skeletal muscle. These results suggested that ryanodine receptor of the SR of the eel skeletal muscle is showing some similar properties with that of mammalian skeletal muscle, but might be an another isotype channel having two bands which is less sensitive to AMP, not cross-reacted with antisera against rat RyR, and not inhibited by high concentration of calcium.

• The Journal of Information Technology
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• v.3 no.4
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• pp.23-40
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• 2000

Because the international trading community has witnessed a number of significant technical and commercial changes, documentary credit practices will quite clearly need to be updated to meet both the business and technical demands of the latest commercial and technical developments in international trade. The potential for developing "paperless credit" should, therefore, be examined. TradeCard is a B2B(business-to-business) e-commerce infrastructure that enables buyers and sellers to conduct and settle international trade transactions securely over the Internet and objective of TradeCard is to provide a secure, reliable cost-effective and user-friendly solution for conducting and settling international trade transactions. This paper analyzes the reviews of TradeCard by Electronic Message and the various problems which come to application of TradeCard, with particular attention to existing international frameworks for Payment systems based on Documentary Credit.ry Credit.

• Journal of the Korean Society of Clothing and Textiles
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• v.38 no.6
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• pp.929-941
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• 2014

Dyeability of Indian natural dyestuffs to fabrics was investigated. Indian dyestuffs are more inexpensive than domestic dyestuffs purchased at oriental medicine stores. We studied the dyeability of madder, marigold, and pomegranate imported from India on cotton, silk, and wool fabrics. Dyebaths of a combination ratio of 80:20, 60:40, 50:50, 40:60 and 20:80 of madder and marigold, fabrics with orange colors were dyed. To evaluate the dyeability of dyed fabrics, K/S values, Munsell color values and CIE L, a, b, ${\Delta}E$ were measured. The dyeability of fabrics dyed in dyebaths of pH 4 and pH 7 were higher than pH 10. The dyeability in pH 4 was better than pH 7. Silk fabrics dyed with madder and marigold showed good dyeability and wool fabrics showed good affinity in madder dyebath and fair affinity in marigold dyebath. Cotton fabrics showed fair affinity in a marigold dyebath of pH 4 and pH 7. Cotton fabrics dyed with a 60:40 ratio of madder and marigold showed 4.76YR of Munsell color value. Silk Fabrics dyed with a 40:60 ratio showed 4.76YR and wool fabrics dyed with 20:80 ratio showed 5.57RY. The ratios produced the closest colors to 5.0YR of orange. This result indicated that marigold had a more powerful effect on cotton fabrics while madder was stronger on wool fabrics. Fading grades of washing colorfastness of wool and silk fabrics dyed in mixed dyebaths were higher than 3.5-4.0 and higher than homogeneous dyebaths. Staining grades of washing colorfastness of all dyed fabrics were between grades 3.5-5.0. Colorfastness to dry-cleaning was high as grades 4.0-5.0 in all of dyed fabrics. Colorfastness to light of dyed fabrics showed a fair grade of 3.5-5.0; in addition, wool fabrics showed lower grades than silk and cotton fabrics.

• Journal of Astronomy and Space Sciences
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• v.3 no.1
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• pp.1-13
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• 1986

A long-term project(ten-year ; 1982-92) for the photoeletric observation in the UBV passbands of selected eclipsing binaries with P$\geq$10 days has initiated at Yonsei University Observatory using 40-cm and 61-cm reflectors. the instrumentatio used and the observation techniques and the reduction procedures applied to this investiagation are described. Out of 39 program stars, successful results have been obtained during our first two years for the 11 stars listed below, with the orbital period in days in the parenthesis, and the incomplete light curves of these stars are presented. SX Cas (36.57) AQ Cas (11.72) UU Cnc (96.71) RY Gem (9.30) V373 Cas (13.42) NY Cep (15.28) RX Cas (32.32) V396 Cas (15.28) ZZ Cnc (25.60) Zet Aur (982.2) Eps Aur (9885.) For the rest of the stars, the observations made in the first two years are not sufficient to attempt any meaning light curve construction ; some of the data are too fragmented and others show large scatter.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.65 no.8
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• pp.1326-1333
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• 2016

It is important to clear the fault and prevent resulting in damage to power system. Although the frequency of generator internal fault is relatively low, it can lead to incalculable damage to power system as well as generator. Especially, loss of field on generator can cause the generator to lose synchronism for a short time if it is not removed promptly. Therefore, it is needed to conduct research on loss of field relay for detecting or clearing the loss of field. However, the setting of the relay may vary in generator operator or engineer, and the relay is not coordinated well with other elements associated with loss of field. In this paper, we address specifically the coordination of positive offset mho loss of field relay which is one of the protection schemes for loss of field. Computer simulations are performed by using ElectroMagnetic Transients Program-Restructured Version (EMTP-RV) based on actual data.