• Title/Summary/Keyword: 4-derivation

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Determination of Nebramycin Factor 2,4,5,5',6 and Kanamycin A in Fermentation Broth of Streptoalloteichus hindustanus ATCC 31218 Mutant Using 2,4-Dinitrofluorobenzene(DNFB) as a Derivatizing Agent by High Performance Liquid Chromatography (HPLC법에 의한 2,4-디니트로플루오로벤젠을 유도체화제로 한 Streptoalloteichus hindustanus ATCC 31218 변이균의 배양액 중 네브라마이신 펙터 2,4,5,5',6, 가나마이신 A 분석)

  • 박영근;박명용;김승철;양호길
    • YAKHAK HOEJI
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    • v.37 no.1
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    • pp.1-8
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    • 1993
  • A procedure for the high-performance liquid chromatographic determination of Nebramycin factors in fermentation broth of Streptoalloteichus hindustanus ATCC 31218 mutant was investigated using pre-column derivatization and LTV detection. The method is based on pre-column derivatization of Nebramycin factors with 2,4-dinitrofluorobenzene(DNFB) in the presence of Tris (hydroxymethyl)aminoethane. The chromatographic separation of derivatives of Nebramycin factors and unknown impurities is achieved using reversed-phase column (NOVA-PAK $C_{18}$, Waters Co.) and AcCN : H$_{2}$O : AcOH (53.0:46.5:0.5) as a mobile phase. The mixture of these derivatives were separated within 35 minutes and the optimum wavelength($\lambda_{max}$ ) of the UV detector was 353 nm. The linearity of response for derivatives of Nebramycin factors is demonstrated for concentrations up to 500 $\mu\textrm{g}$/ml and the relative standard deviation is less than 0.79%. Detection limit was 1.67 ng for the 10 $\mu\textrm{l}$ sample volume employed.

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Studies on the Derivation Basis of Surface Water Quality Standards for Human Health Protection and Drinking Water Standards in Foreign Countries: 1,4-Dioxane, Formaldehyde, and Hexachlorobenzene (인체건강보호를 위한 수질환경 및 먹는물 기준에 대한 외국의 도출근거 연구 : 1,4-Dioxane, Formaldehyde, Hexachlorobenzene를 대상으로)

  • Kwak, Jin Il;An, Youn-Joo
    • Journal of Korean Society on Water Environment
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    • v.29 no.6
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    • pp.842-846
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    • 2013
  • In 2012, the Korean Ministry of Environment (MOE) added 3 new water quality standards for the protection of human health; specifically, regarding 1,4-dioxane, formaldehyde, and hexachlorobenzene. In this study, we assimilated the water quality standards of these 3 substances from other countries, with respect to surface water quality standards for human health protection and drinking water standards. We subsequently investigated how these standard values were derived. 1,4-Dioxane is managed as an environmental standard for human health in Japan, and as a drinking water quality standard in WHO, New Zealand, and Japan with respect to both carcinogenic and non-carcinogenic effects. In New York, the oncogenic effects of formaldehyde in drinking water intake is considered, whereas WHO, Australia, New Zealand, and Japan also assess the non-carcinogenic effects of formaldehyde when setting their standards. USEPA and New York have a water quality standard for human health protection with respect to hexachlorobenzene based on carcinogenic effects. This study focuses on deriving water quality standards for the 3 new substances, or obtaining baseline information to revise the values of existing substances in the future.

A Statistical Study Evaporation tn DAEGU Area (대구지방의 증발량에 대한 통계학적 연구)

  • 김영기
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.15 no.4
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    • pp.3160-3169
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    • 1973
  • Rainfall, evaporation, and permeability of water are the most important factors in determining the demand of water. The Daegu area has only a meteorologi observatory and there is not sufficient data for adapting the advanced method for derivation of the estimated of evaporation in the Daegu area. However, by using available data, the writer devoted his great effort in deriving the most reasonable formula applicable to the Daegu area and it is adaptable for various purposes such as industry and estimation of groundwater etc. The data used in this study was the monthly amount of evaporation of the Daegu area for the past 13 years(1960 to 1970). A year can be divided into two groups by relative degrees of evaporation in this area: the first group (less evaporation) is January, February, March, October, November, and December, and the second (more evaporation) is April, May, June, July, August, and September. The amount of evaporation of the two groups were statistically treated by the theory of probability for derivation of estimated formula of evaporation. The formula derved is believed to fully consider. The characteristic hydrological environment of this area as the following shows: log(x+3)=0.8963+0.1125$\xi$..........(4, 5, 6, 7, 8, 9 month) log(x-0.7)=0.2051+0.3023$\xi$..........(1, 2, 3, 10, 11, 12 month) This study obtained the above formula of probability of the monthly evaporation of this area by using the relation: $F_(x)=\frac{1}{{\surd}{\pi}}\int\limits_{-\infty}^{\xi}e^{-\xi2}d{\xi}\;{\xi}=alog_{\alpha}({\frac{x_0+b'}{x_0+b})\;(-b<x<{\infty})$ $$log(x_0+b)=0.80961$ $$\frac{1}{a}=\sqrt{\frac{2N}{N-1}}\;Sx=0.1125$$ $$b=\frac{1}{m}\sum\limits_{i-I}^{m}b_s=3.14$$ $$S_x=\sqrt{\frac{1}{N}\sum\limits_{i-I}^{N}\{log(x_i+b)\}^2-\{log(x_i+b)\}^2}=0.0791$$ (4, 5, 6, 7, 8, 9 month) This formula may be advantageously applied to estimation of evaporation in the Daegu area. Notation for general terms has been denoted by following: $W_(x)$: probability of occurance. $$W_(x)=\int_x^{\infty}f(x)dx$$ P : probability $$P=\frac{N!}{t!(N-t)}{F_i^{N-{\pi}}(1-F_i)^l$$ $$F_{\eta}:\; Thomas\;plot\;F_{\eta}=(1-\frac{n}{N+1})$$ $X_l\;X_i$: maximun, minimum value of total number of sample size(other notation for general terms was used as needed)

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A Method for Reachability Graph Derivation from Timed Automata (시간적 오토마타에서 도달성 그래프의 도출 기법)

  • Park, Chan-Min
    • Journal of KIISE:Information Networking
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    • v.27 no.4
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    • pp.482-488
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    • 2000
  • 프로토콜 검증에 있어서 reachability analysis를 사용하는 방법은 널리 알려지고 많이 사용되는 방법이다. Reachability analysis를 통한 검증 방법에 있어서는 프로토콜의 동작을 나타내는 reachability graph의 생성이 중요하다. 그러나 시간이 관련되는 경우 발생하는 상태 수 폭증또는 시간의 실수적 성질의 문제가 있어서 reachability graph의 생성에 큰 장애가 되어왔다. 실시간 시스템이나 프로토콜에 있어서 타이머는 대기 시간의 한계를 지정하거나 일정한 시간간격을 시스템에 알릴 수 있도록 하는 등 시간의 상대적 기준점으로 사용되며 이외에도 여러 가지 용도로 타이머를 사용하게 되어 프로토콜을 모델링하는데 있어서 타이머는 불가결하다. 본 논문에서는 타이머를 모델링하지 못하며 통신 프로토콜과 그 환경을 모델링하는데 적절치 못하던 기존의 방법과 달리 타이머를 포함하며 통신 프로토콜과 그환경을 적절히 표현할 수 있도록 시스템을 기술하는 방법과 함께 상대적 시간 개념으로 천이와 타이머를 다루는 방법을 통해서 시간개념과 타이머가 들어있는 프로토콜의 reachability graph를 유도하는 방법을 제안하였다. 제안한 방법을 통해서 기존방법으로는 찾아낼수 없었던 시스템의 오류를 찾아낼 수 있었다. 이를 통해 보다 실제 프로토콜에 가깝에 모델링하고 검증할 수 있는 기반을 마련하고 있다.

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Structural Behavior of Concrete Pavement Due to Temperature Variations (온도변화에 의한 콘크리트 포장도로의 구조 거동 연구)

  • 조병완
    • Computational Structural Engineering
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    • v.4 no.2
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    • pp.95-102
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    • 1991
  • Some sections of concrete highway pavements have been viewed with great concern by highway officials and engineers due to the severe cracking and failure problems. This is mainly due to the traffic loads in addition to temperature variations between top and bottom of concrete slab, which cause the concrete slab to curl up and down depending on the thermal gradient, respectively. Subsequently, a major consideration was given to the derivation of stiffness matrix and equivalent nodal loads due to the uniform gravity load, temperature and shrinkage of concrete. And the structural behavior of concrete highway pavement due to the temperature variations throughout the nations has been emphasized.

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Resonant response of spar-type floating platform in coupled heave and pitch motion

  • Choi, E.Y.;Cho, J.R.;Jeong, W.B.
    • Structural Engineering and Mechanics
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    • v.65 no.5
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    • pp.513-521
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    • 2018
  • In this paper, the resonance response of spar-type floating platform in coupled heave and pitch motion is investigated using a CPU time-effective numerical method. A coupled nonlinear 2-DOF equation of motion is derived based on the potential wave theory and the rigid-body hydrodynamics. The transient responses are solved by the fourth-order Runge-Kutta (RK4) method and transformed to the frequency responses by the digital Fourier transform (DFT), and the first-order approximation of heave response is analytically derived. Through the numerical experiments, the theoretical derivation and the numerical formulation are verified from the comparison with the commercial software AQWA. And, the frequencies of resonance arising from the nonlinear coupling between heave and pitch motions are investigated and justified from the comparison with the analytically derived first-order approximation of heave response.

Development of a New Methodology to find the Expected Busy Periods for Controllable M/G/1 Queueing Models Operating under the Multi-variable Operating Policies: Concepts and applications to the dyadic policies

  • Rhee, Hahn-Kyou
    • Journal of Korean Institute of Industrial Engineers
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    • v.23 no.4
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    • pp.729-739
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    • 1997
  • In this paper, steady-state controllable M/G/1 queueing systems operating under the dyadic policies are considered. A new method to obtain the expected busy period when the D-policy is involved in system operation, is developed. This new method requires derivation of so called 'the pseudo probability density function' of the busy period for the system under consideration, which is completely different from its actual probability density function. However, the proposed pseudo probability density function does generate the correct expected busy period through simple procedures.

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An Analysis of Group Key Agreement Schemes based on the Bellare-Rogaway Model in Multi-party Setting

  • Lim, Meng-Hui;Goi, Bok-Min;Lee, Sang-Gon
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.5 no.4
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    • pp.822-839
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    • 2011
  • Group key agreement protocols derive a shared secret key for a group of users to ensure data confidentiality or/and integrity among the users in the subsequent communications. In this paper, we inspect two group key agreement schemes which have been proposed by Shi et al. and Zheng et al. in 2005 and 2007 respectively. Although both schemes were claimed to be secure in a heuristic way, we reveal several flaws using the Bellare-Rogaway security model extended to multi-party setting by Bresson et al. These flaws are found to be originated from inappropriate selection of key derivation function, inadvertent exclusion of partners' identities from the protocol specification and insufficient consideration in preserving known temporary information security and key freshness properties. Furthermore, we suggest and discuss proper countermeasures to address such flaws.

Performance Evaluation of Registration Schemes in Mobile Communication Network: Movement-Based Registration and Distance-Based Registration (이동통신망에서 위치등록 방법의 성능평가: 이동기준 위치등록과 거리기준 위치등록)

  • Ryu, Byung-Han;Baek, Jang-Hyun
    • IE interfaces
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    • v.15 no.4
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    • pp.401-408
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    • 2002
  • An efficient mobility management for mobile stations plays an important role in mobile communication network. In this study, we consider movement-based registration(MBR) and distance-based registration(DBR). Analytical models based on 2-dimensional random walk in hexagonal cell configuration are considered to analyze the performance of MBR and DBR. Especially, we focus on the derivation of the registration cost of DBR scheme by using two analytical methods and then show that DBR always outperforms MBR. Numerical results are provided to demonstrate optimal condition under various circumstances.

A Study of the Quantitative Relationship of Charge-Density Changes and the Design Area of a Fabricated Solar Cell

  • Jeon, Kyeong-Nam;Kim, Seon-Hun;Kim, Hoy-Jin;Kim, In-Sung;Kim, Sang-Hyun
    • Transactions on Electrical and Electronic Materials
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    • v.12 no.5
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    • pp.204-208
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    • 2011
  • In this paper, the design area of a fabricated solar cell has been analyzed with respect to its charge density. The mathematical calculation used for charge-density derivation was obtained from the 2001 version of a MATHCAD program. The parameter range for the calculations was ${\pm}1{\times}10^{17}cm^{-3}$, which is in the normal parameter range for n-type doping impurities ($7.0{\times}10^{17}cm^{-3}$) and also for p-type impurities ($4.0{\times}10^{17}cm^{-3}$). Therefore, it can be said that the fabricated solar-cell design area has a direct effect on charge-density changes.