• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.16 no.4
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• pp.31-40
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• 2016

In vehicular ad-hoc networks (VANETs), vehicles sense information on emergency incidents (e.g., accidents, unexpected road conditions, etc.) and propagate this information to following vehicles and a server to share the information. However, this process of emergency message propagation is based on multiple broadcast messages and can lead to broadcast storms. To address this issue, in this work, we use a novel approach to detect the vehicles that are farthest away but within communication range of the transmitting vehicle. Specifically, we discuss a signal-to-noise ratio (SNR)-based linear back-off (SLB) scheme where vehicles implicitly detect their relative locations to the transmitter with respect to the SNR of the received packets. Once the relative locations are detected, nodes that are farther away will set a relatively shorter back-off to prioritize its forwarding process so that other vehicles can suppress their transmissions based on packet overhearing. We evaluate SLB using a realistic simulation environment which consists of a NS-3 VANET simulation environment, a software-based WiFi-IP gateway, and an ITS server operating on a separate machine. Comparisons with other broadcasting-based schemes indicate that SLB successfully propagates emergency messages with latencies and hop counts that is close to the experimental optimal while reducing the number of transmissions by as much as 1/20.

• IMMUNE NETWORK
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• v.1 no.3
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• pp.221-229
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• 2001

Background: Inactivation of tumor suppressor genes is believed to be important in the development of many human malignancies. Recently, several lines of evidence have indicated that the wild type p53 gene located at 17p13.3, may function as a tumor suppressor gene and that a mutant p53 gene could promote transformation by inactivating normal p53 function in a dominant negative fashion. These broad spectrum of p53 mutation in human cancers provide that mutant p53 and their protein may be potential targets of tumor diagnostic and therapeutic interventions. Method: Colony formation was performed to investigate growth suppressional ability. p53 expression pattern was examined by western blot and p53-mediated transactivation ability was assessed by CAT activity. SNU C2A cells were observed in apoptotic aspects induced by etoposide and $H_2O_2$ treatment, detecting sensitivity on agent, DNA fragmentation through agarose gel, chromatin condensation by fluorescence microscope, and cell cycle distribution by FACS. Result: 1) p53 mutant his179arg ($histidine{\rightarrow}arginine$) detected in SNU C2A cells lost transcriptional activity and growth suppression ability, showing dominant negative effect on its wild type p53. 2) Etoposide-treated SNU C2A cells induced apoptosis, exhibiting dramatic reduction of cell growth, DNA fragmentation, nuclear condensation formation of apoptotic body and increment of sub-G1 cell fraction. 3) Etoposide and $H_2O_2$-treated SNU C2A cells have no high increase of p53 expression and overexpressed p53 protein changed localization, from cytoplasm to nucleus. Also, p53-mediated transcriptional activity was increased by agents-treatment. Conclusion: SNU C2A cells coexpress wild-type and mutant p53 protein induced apoptosis in the condition on DNA damage, through localizational shift from cytoplasm to nucleus of p53 protein rather than the induction of p53 protein. SNU C2A cells derived mutant p53 his179arg abrogated both the growth supression ability and transactivational activity, showing inhibition effect on transcriptional activity of wild type p53, but did not repress the activity of wild type p53 in SNU C2A cells owing to dominant activity of wild type. These cell condition may provide new gene therapeutic implications leading effective antiproliferation of cell when mutant and wild-type p53 protein were co-expressed in cell.

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.1-8
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• 2013

COP9 signalosome (CSN), which is originally identified as the regulator of the photomorphogenic development in plant, is highly conserved protein complex in diverse eukaryotic organisms. Most eukaryotic CSN complex is composed of 8 subunits, which is structurally and functionally similar to the lid subunit of 26S proteasome and eIF3 translation initiation complex. CSN play important functions in the regulation of cell cycle and checkpoint response by controlling Cullin-Ring E3 ubiquitin ligases (CRL) activities. CSN exhibits an isopeptidase activity which cleaves the neddylated moiety of cullin components. In fission yeast, S-phase cell cycle progression was delayed and the sensitivity to g-ray or UV was increased in CSN1 and CSN2 deletion mutants, indicating that yeast CSN is also involved in the checkpoint regulation. CSN in fungal system more closely resembles that of the higher organisms in the structure and assembly of their components. Functionally, CSN is associated with the regulation of conidiation rhythms in Neurospora crassa and the sexual development in Aspsergillus nidulans. Recent studies also revealed that CSN functions as an essential cell cycle regulator, playing key roles in the regulation of DNA replication and DNA damage response in Aspergillus. Overall, CSN of microorganisms, such as fission yeast and fungi, share functionally common aspects with higher organisms, implying that they can be useful tools to study the role of CSN in the CRL-mediated diverse cellular activities.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.29 no.6
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• pp.379-391
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• 2003

The present study was undertaken to evaluate bone regenerative capacity around titanium screw implants placed in irradiated rat's tibiae. At one week after single 15-Gy dose irradiation, miniaturized titanium screw implants were inserted into anterior aspect of the upper tibia of rats weighing 200-250g. Seventy rats were involved: 35 rats were control and 35 rats radiation group. The rats were killed at different intervals as 1, 2, 3, 4, 6, 8, 12 weeks after implantation for histologic observation, histomorphometric analysis and immunohistochemical study with fibronectin and CD34 antibody. 1. Histologically, various stages of bone maturation and ossification can be seen at 4 weeks and regenerated bone close to edges demonstrates more advanced calcification, and network of new bone are well formed at 12 weeks in non-irradiated group. In contrast, active bone formation with increased contact of newly formed bone to implant surface was noted at 4 weeks and a significant amount of new bone formation and bone-implant contact is oberved at 12 weeks in irradiated group. 2. Histomorphometrical analysis confirmed these histologic findings. A significant difference in implant-bone contact and bone density was measured between the control and radiation group. Mean MBD was 62.2% in control group and 27.5% in radiation group, mean MBIC was 86.6% in control group and 47.7% in radiation group, and mean TBIC was 87.3% in control group and 45.6% in radiation group at 12 weeks after implantation. 3. In immunohistochemical study with fibronectin and CD34, radiation reduced hematopoietic progenitor cells severely and disturbed differentiation of osteoblast in bone marrow. The results of this study revealed bone healing capacity around implant after radiation therapy was severely impaired and irradiation reduces the capacity for osseointegration of titanium implants. Many factors including radiation dose, period between radiation and implantation, bone quality, time elapse between first and second surgery, type of prosthetics and hyperbaric oxygen therapy must be considered carefully in postradiation implantation.

• The KIPS Transactions:PartC
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• v.10C no.7
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• pp.943-954
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• 2003

The mobile telecommunication system has been growing exponentially after 1990s due to the high population in a city and the growth of mobile user. In this time, the current mobile system mainly concentrates on the voice communication. However, in the next generation, mobile users want to get very diverse services via mobile terminal such as the Internet access, web access, multimedia communication, and etc. For this reason, the next generation system, such as the UMTS (Universal Mobile Telecommunication Services) system, has to support the packet data service and it will play the major role in the system. By the way, since the Web service is based on TCP, most of the Internet traffic TCP traffic. Therefore, efficient transmission of TCP traffic will take very important role in the performance of packet data service. There are many researches about improving TCP performance over wireless network. In those schemes, the UMTS system adapts the link layer retransmission scheme. However, there are rarely studies about the exact performance of the link layer retransmission scheme in the face of dynamic changes of wireless environment over the UMTS system. The dynamic changes of wireless environment, such as wireless bandwidth, can degrade TCP performance directly. So, in this paper, we simulate and analyze the TCP performance in the UMTS system with dynamic wireless environments. Then, we propose a simple scheme for minimizing TCP performance degradation. As a result of simulation, we can find that when wireless environment is changed dynamically, the probability of TCP timeout is increased, and the TCP performance is degraded very much. In this situation, the proposed simple scheme shows good performance. It saves wireless resources and reduces the degradation of TCP performance without large overhead of the base station.

• Geophysics and Geophysical Exploration
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• v.15 no.3
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• pp.144-149
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• 2012

Korea Polar Research Institute (KOPRI) has successfully installed two autonomous very broadband three-component seismic stations at the King George Island (KGI), Antarctica, during the 24th KOPRI Antarctic Summer Expedition (2010 ~ 2011). The seismic observation system is originally designed by the Incorporated Research Institutions for Seismology Program for Array Seismic Studies of the Continental Lithosphere Instrument Center, which is fully compatible with the Polar Earth Observing Network seismic system. The installation is to achieve the following major goals: 1. Monitoring local earthquakes and icequakes in and around the KGI, 2. Validating the robustness of seismic system operation under harsh environment. For further intensive studies, we plan to move and install them adding a couple more stations at ice shelf system, e.g., Larsen Ice Shelf System, Antarctica, in 2013 to figure out ice dynamics and physical interaction between lithosphere and cryosphere. In this article, we evaluate seismic station performance and characteristics by examining ambient noise, and provide operational system information such as frequency response and State-Of-Health information.

• Restorative Dentistry and Endodontics
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• v.24 no.1
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• pp.100-115
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• 1999

The purpose of this study was to investigate the distribution of calcitonin gene-related peptide containing nerve fibers in rat pulp after dentinl injury by means of immunohistochemistry and confocal laser scanning microscope. The Spague-Dawley rats weighing about 250-300gm were used. The animals were devided into normal control and experimental groups. Experimental animals were sacrified 1, 2, 4, 7, 10, 21days after dentinal injury (dentin cutting, and then acid etching with 35% phosphoric acid) on the maxillary molar teeth. The maxillary teeth and alveolar bone were removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4), then were decalcified with 15% formic acid for 10 days. Serial frozen $50{\mu}m$ thick sections were cut on a cryostat. The rabbit CGRP antibody was used as a primary antibody with a dilution of 1:2000 in 0.01M PB. The sections were incubated for 48 hours at $4^{\circ}C$, and placed into biotinylated antirabbit Ig G as a secondary anti body with dilution of 1:200 in 0.01M PB and incubated in ABC(avidin-biotin complex). The peroxidase reaction was visualized by incubating the sections in 0.05% 3,3 diaminobenzidine tetrahydrochloride containing 0.02% $H_2O_2$. For the confocal laser scanning microscopic examination, Primary antibody reaction was same as immunoperoxidase stainning, but fluorescein isothiocyanate(FITC)-conjugate antirabbit IgG as a secondary antibody was used. The confocal laser scanning microscope was used for the examination. A series of images of optical sections was collected with a 20x objective at $3{\mu}m$ intervals throughout the depth of specimen. FITC fluerescence was registrated through a 488nm and 568nm excitation filter, and images were saved on optical disk. The stereoscopic images and three dimentionnal images were reconstructed by computer software, and then were analyzed. The results were as follows : 1. In normal control group, CGRP containing nerve fibers were coursed through the root with very little branching, and then formed a dense network of terminals in coronal pulp. 2. A slight increase in CGRP containing nerve fibers at 1 and 2day postinjury was noted subjacent to the injury site. In the 4day group, there were an extensive increase in the number of reactive fibers, followed by a partial return toward normal levels at 7~10 day postinjury, and return by 21days. 3. The sprouting of the CGRP containing nerve fibers was evident within 2day after dentinal injury, and by 4days there was a maximal increased, but was decreased at 7days and returned to normal 10~21 day postinjury. 4. In confocal laser scanning microscopic exammination, the distinct distribution pattern and sprouting reaction of CGRP containing nerve fibers were observed in stereoscopic images and three dimentional images. These results suggest that CGRP containing nerve fiber can be important role in the response to dental injury and pain regulation.

• IMMUNE NETWORK
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• v.1 no.1
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• pp.77-86
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• 2001

Background: Hepatitis C virus(HCV), a family of Flaviviridae, has a host cell-derived envelope containing a positive-stranded RNA genome, and has been known as the maj or etiological agent for chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. There remains a need to dissect a molecular mechanism of pathogenesis for the development of therapeutic and effective preventive measure for HCV. Identification of cellular receptor is of central importance not only to understand the viral pathogenesis, but also to exploit strategies for prevention of HCV. This study was aimed at identifying peptide mimotopes inhibiting the binding of E2 protein of HCV to MOLT-4 cell. Methods: In this study, phage peptide library displaying a random peptides consisting of 7 or 12 random peptides was employed in order to pan against E2 protein. Free HCV particles were separated from the immune complex forms by immunoprecipitation using anti-human IgG antibody, and used for HCV-capture ELISA. To identify the peptides inhibiting E2-binding to MOLT-4 cells, E2 protein was subj ect to bind to MOLT-4 cells under the competition with phage peptides. Results: Several phage peptides were selected for their specific binding to E2 protein, which showed the conserved sequence of SHFWRAP from 3 different peptide sequences. They were also able to recognize the HCV particles in the sera of HCV patients captured by monoclonal antibody against E2 protein. Two of them, showing peptide sequence of HLGPWMSHWFQR and WAPPLERSSLFY respectively, were revealed to inhibit the binding of E2 protein to MOLT-4 cell efficiently in dose dependent mode. However, few membrane-associated receptor candidates were seen using Fasta3 programe for homology search with these peptides. Conclusion: Phage peptides containing HLGPWMSHWFQR and WAPPLERSSLFY respectively, showed the inhibition of E2-binding to MOLT-4 cells. However, they did not reveal any homologues to cellular receptors from GenBank database. In further study, cellular receptor could be identified through the screening of cDNA library from MOLT-4 or hepatocytes using antibodies against these peptide mimotopes.

• IMMUNE NETWORK
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• v.1 no.3
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• pp.213-220
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• 2001

Background: A previous study has shown that Euonymus alatus (EA) has an antidotic activities against inflammation, suggesting possibility that EA can exert this beneficial effects to liver injury by an initial protection against drug-induced hepatocyte demage. The present study was undertaken to evaluate the protective effect of EA-extract on experimentally induced hepatitis in ICR mice and to investigate some mechanisms responsible for its action. Methods: Water EA extract was used in this experiments. The mice received i.p. a dose of 700 mg/kg galactosamine (GalN) together with $5{\mu}g/kg$ of endotoxin (LPS), or received i.v. 12 mg/kg of concanavalin A (Con A). EA (4 mg/mouse) was administrated on day -2, -1 and 0 before induction of liver injury. Liver injury was assessed by measurement of serum alanin amino-transferase (SGPT) levels on 9 hr after GaIN.LPS, or 8 hr after con A administration. Results: Treatment with either GaIN or LPS alone did not cause hepatitis. However, simultaneous administration of GalN and LPS to mice resulted in LPS-dose dependent fulminant hepatitis. GaLN/LPS-induced liver injury was reduced when mice were given EA for 3 days before induction. This preventive effect of Ea was more prominent when EA was given by intraperitoneal route rather then by oral route. Pretreatment of EA or dexamethasone inhibited significantly $TNF{\alpha}$ production in GalL/LPS-injured mice. However, EA-treatment did not influence $TNF{\alpha}$-induced hepatitis in GalN-sensitized mice, suggesting that $TNF{\alpha}$ is likely to act as one of final mediators of endotoxin action and the protective effect of EA might be manifested chiefly by inhibition of endotoxin-induced $TNF{\alpha}$ production, not by blocking the $TNF{\alpha}$-action. Injection of Con A into mice evoked remarkable liver injury in a dose dependent fashion. This liver damage was reduced by EA-pretreatment. Dexamethasone significantly reduced both GalL/LPS-induced and Con A-induced liver damages, showing synergism with EA. However, indomethacin reduced only GalN/ LPS-induced hepatitis, not for Con A-induced hepatitis. Conclusion: These results led to the conclusion that EA may be able to contribute at least in part to prevent the drug-induced hepatotoxicity, and that its anti-hepatitis effects might be manifested directly by modulation of endogenous mediators, such as leukotriese D4, $TNF{\alpha}$ and free radical, and indirectly by regulation of immune mediated responses. Also these results suggested that EA could be developed as a potential antidotic agent.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.1565-1569
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• 2008

지난 수십 년 동안, 아시아 태평양 지역 각국은 수자원 접근성 및 인프라의 확충을 포함한 물관리의 다양한 측면에서 현저히 발전하였다고 평가되고 있다. 그러나 급속한 인구 증가 및 경제 성장, 도시화, 기존 수자원의 고갈 등은 아태 지역의 물 수요가 여전히 충족시키지 못하는 원인이다. 또한 기후변화로 인한 환경적 변화를 고려하여 모든 수자원정책의 입안 및 계획이 이루어져야 한다. 경제적 성장률은 전반적으로 높았지만 빈곤 문제는 역시 도시나 농촌을 구분할 것 없이 만성적인 문제로 남아 있으며, 아태 지역 개도국 인구의 16%가 영양실조 상태이며, 2015년까지 그 수를 반감하자는 밀레니엄 개발목표(Millenium Development Goals, MDGs) 달성은 아직 갈 길이 멀다. 또한 아태 지역은 물 관련 재해에 대해 전 세계에서 가장 취약한 지역이며 그로 인한 지속가능한 발전이 지체되고 있다. 1960년부터 2006년까지 물 관련 재해로 인한 전 세계 사상자의 80%에 달하는 60만 명의 사상이 아태 지역에서 발생하였으며, 80억 달러의 경제적 손실이 있었다. 홍수, 가뭄, 쓰나미, 폭풍, 해일 등으로 인한 사회경제적 피해는 최근 들어 더욱 확산되고 있다. 지난 10년간의 급속한 인구 증가로 많은 인구가 범람지대 및 기타 재해 취약 지역에 거주하게 되었으며, 이는 물 인프라 확충을 위한 많은 투자를 요하고 있다. 2006년 3월 멕시코에서 개최된 제4차 세계물포럼의 지역별 추진과정에서 아태 지역은 생태계, 인구, 정치적 제도 및 사회경제적 환경 등 각국의 다양성이 이와 같은 공통된 물문제 해결에 기반이 될 수 있는 요소임에 공감하고 지속적인 수자원 확보와 올바른 수자원관리에 공동의 노력을 기울여 효율적인 메커니즘을 구성을 통해 아태 지역의 사회경제적 발전을 촉구하기로 하였다. 이에 아시아 태평양 물포럼(Asia-Pacific Water Forum)이라는 새로운 이니셔티브가 구성되었고 물 관련 재원 조달 및 인프라 확보를 통해 국경을 초월하는 협력을 증진하고 재해 관리와 조화로운 수자원 개발과 생태 보호등을 위한 일관된 수자원정책 수립과 프로그램 개발 등을 목표로 설정하여 활동을 시작하였다. 아시아 태평양 물정상회의(이하 아태 물정상회의, Asia-Pacific Water Summit(APWS))는 이러한 목표 달성을 구체화하기 위해 각국의 지도자 및 정책입안자에 밀레니엄 개발목표(MDGs) 달성을 위해 물 문제가 얼마나 중요한지 부각시키고 실질적인 정책 결정에 기여하기 위해 제안되었다. 제1차 아태 물정상회의가 2007년 12월 3-4일 양일에 걸쳐 일본 벳부에서 개최되었고, 2008년 G8 정상회담이나 2009년 제5차 세계물포럼에 직접적으로 연계되어 지역 협력을 주도한 가운데 아태 지역 뿐 아니라 전 지구적으로 인류 및 사회 발전을 위한 이정표로 새로운 수자원 네트워크 발돋움의 계기가 되었다.