IMMUNE NETWORK
- Volume 1 Issue 3
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- Pages.221-229
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- 2001
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
Induction of apoptosis by etoposide treatment in colon cancer cell line SNU C2A
대장암 세포주 SNU C2A에서 etoposide 처리에 의한 apoptosis 유도
- Jung, Ji-Yeon (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
- Na, Yun-sook (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
- Jung, Ho-Chul (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
- Oh, Sang-Jin (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
- 정지연 (전남대학교 자연과학대학 생명과학부) ;
- 나윤숙 (전남대학교 자연과학대학 생명과학부) ;
- 정호철 (전남대학교 자연과학대학 생명과학부) ;
- 오상진 (전남대학교 자연과학대학 생명과학부)
- Published : 2001.12.31
Abstract
Background: Inactivation of tumor suppressor genes is believed to be important in the development of many human malignancies. Recently, several lines of evidence have indicated that the wild type p53 gene located at 17p13.3, may function as a tumor suppressor gene and that a mutant p53 gene could promote transformation by inactivating normal p53 function in a dominant negative fashion. These broad spectrum of p53 mutation in human cancers provide that mutant p53 and their protein may be potential targets of tumor diagnostic and therapeutic interventions. Method: Colony formation was performed to investigate growth suppressional ability. p53 expression pattern was examined by western blot and p53-mediated transactivation ability was assessed by CAT activity. SNU C2A cells were observed in apoptotic aspects induced by etoposide and
Keywords
- p53 mutation;
- apoptosis;
- transcriptional activity;
- growth suppression ability;
- SNU C2A cell;
- dominant negative inhibition