• The Journal of Korean Institute of Communications and Information Sciences
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• v.11 no.3
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• pp.172-178
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• 1986

The recent interest in the design of filters is motivatied by the fact that such filter can be fully integrated using standard metal-oxide-semiconductor processing technology. This is due to replacing all the resistors in the active RC filter network by the switched capacitors. The voltage gain of a SC filter depends only on the rations of capacitance and these ratios can be obtained and maintained to high accuracy. Therefore, it is known that a switched capacitor is much better than a resistor in temperature and linearity characteristics. This paper proposed a programmable SC filter and proved the fact that ${omega}_0$ Q and G of this circuit can be controlled by digital signal. Experiments show that SC filter remains the low sensitivities but it can't avoid little influence of parasitic capacitance. As the transfer characteristic of the SC filter is varied with sampling frequency and resistor array, SC filtering technigue can be applied for digital processing, speech analysis and synthesis and so on.

• Molecules and Cells
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• v.40 no.11
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• pp.828-836
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• 2017

Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.9
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• pp.901-908
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• 2013

The introduction of industrial intelligent robot using vision sensor has been interested in automated factory. 2D and 3D vision sensors have used to recognize object and to estimate object pose, which is for packaging parts onto a complete whole. But it is not trivial task due to illumination and various types of objects. Object image has distorted due to illumination that has caused low reliability in recognition. In this paper, recognition method of complex shape object has been proposed. An accurate object region has detected from combined binary image, which has achieved using DoG filter and local adaptive binarization. The object has recognized using neural network, which is trained with sub-divided object class according to object type and rotation angle. Predefined shape model of object and maximal slope have used to estimate the pose of object. The performance has evaluated on ETRI database and recognition rate of 96% has obtained.

• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.51-55
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• 2007

As for satellite programs, the multipurpose satellite 1(KOMPSAT-1) was successfully launched on Dec. 21, 1999 and operated for three years. It is still properly operated even though its life cycle was ended. The development of KOMPSAT-2 (Korea Multipurpose Satellite-2) is near completion and the development of KOMPSAT-3, KOMPSAT-5 and COMS (Communication, Ocean, Meterological Satellite) are proceeding swiftly. In KORDI(Korea Ocean Research and Development Institute), the KOSC (Korea Ocean Satellite Center) construction project is being prepared for acquisition, processing and distribution of sensor data via L-band from GOCI(Geostationary Ocean Color Imager) instrument which is loaded on COMS(Communication, Ocean and Meteorological Satellite); it will be launched in 2000. Ansan(the headquarter of KORDD has been selected for the location of KOSC between 5 proposed sites, because it has the best condition to receive radio wave. The data acquisition system is classified antenna and RF. Antenna is designed to be ${\emptyset}$ 9m cassegrain antenna which has 19.35 $G/T(dB/^{\circ}K)$ at 1.67GHz, RF module, is divided into LNA(Low noise amplifier) and down converter, those are designed to send only horizontal polarization to modem The existing building is re-designed and classified for the KOSC operation concept; computing room, board of electricity, data processing room, operation room Hardware and network facilities have been designed to adapt for efficiency of each functions. The distribution system which is one of the most important systems will be constructed mainly on the internet, and it is also being considered constructing outer data distribution system as a web hosting service for to offering received data to user under an hour.

• Journal of Communications and Networks
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• v.14 no.3
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• pp.336-345
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• 2012

Autonomous system (AS) business relationships and their inference have been widely studied by network researchers in the past. An important application of inferred AS relationships can be the prediction of AS paths between a source and destination AS within a model. However, besides knowing the topology and inferred AS relationships, AS path prediction within a model needs to be understood in order for us to know how we can derive border gateway protocol (BGP) policies from AS relationships. In this paper, we shed light onto the predictive capabilities of AS relationships by investigating whether they can be translated into BGP policies such that inferred AS paths are consistent with real AS paths, e.g., paths observed from BGP routing tables. Our findings indicate that enforcing constraints such as the well-known valley-free property and the widely assumed preference of customer routes always results in a very low consistency for AS path inference. In addition, this is true irrespective of whether customer, peer, or provider routes are preferred. Apparently, applying such constraints eliminates many "correct" paths that are observed in BGP routing tables and that are propagated in a simple shortest path model where AS relationships are ignored. According to our findings, deriving BGP routing policies for predicting with high accuracy AS paths in a model directly from AS relationships is still difficult.

• Korean System Dynamics Review
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• v.14 no.3
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• pp.25-49
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• 2013

As global competition for green car, that is environmentally friendly car, is getting tougher, the governments and the related industries are putting their core efforts in its diffusion. However, the green car sales are disappointing so far. To overcome the gridlock, it is necessary to develop concrete analytical framework to understand the diffusion process. Based on causal loop analysis from the previous work, we have identified main variables and relationships of them in the diffusion process and developed a stock-flow diagram and mathematical formula for the main components. The model would be applied for further quantitative simulation on the diffusion process of green car and other innovative goods as well. Also, we have suggested constructive insights for the policy makers and for the related industries. First, it is important to increase consumers' willingness to consider through marketing and word of mouth to accelerate the diffusion process. Second, in the perspective of the industry, the market share of green car should be increased at the earliest possible stage and this could be done by enhancing each components of green car attractiveness(e.g. price, driving range, social infra). Third, companies should develop a balanced investment between consumer and technology sector through a flexible financial policy. Fourth, the government continuously has the role of investing in the related R&D and social infra building. We expect the green car diffusion model and related formula from the research can provide meaningful tools to analyze the diffusion process of other new and innovative goods based on its deep researched literature review.

• Journal of Ginseng Research
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• v.42 no.4
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• pp.455-462
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• 2018

Background: Ginsenoside Rh2 has been known to enhance the activity of immune cells, as well as to inhibit the growth of tumor cells. Although the repertoire of genes regulated by Rh2 is well-known in many cancer cells, the epigenetic regulation has yet to be determined, especially for comprehensive approaches to detect methylation changes. Methods: The effect of Rh2 on genome-wide DNA methylation changes in breast cancer cells was examined by treating cultured MCF-7 with Rh2. Pyrosequencing analysis was carried out to measure the methylation level of a global methylation marker, LINE1. Genome-wide methylation analysis was carried out to identify epigenetically regulated genes and to elucidate the most prominent signaling pathway affected by Rh2. Apoptosis and proliferation were monitored to examine the cellular effect of Rh2. Results: LINE1 showed induction of hypomethylation at specific CpGs by 1.6-9.1% (p < 0.05). Genome-wide methylation analysis identified the "cell-mediated immune response"-related pathway as the top network. Cell proliferation of MCF-7 was retarded by Rh2 in a dose-dependent manner. Hypermethylated genes such as CASP1, INSL5, and OR52A1 showed downregulation in the Rh2-treated MCF-7, while hypomethylated genes such as CLINT1, ST3GAL4, and C1orf198 showed upregulation. Notably, a higher survival rate was associated with lower expression of INSL5 and OR52A1 in breast cancer patients, while with higher expression of CLINT1. Conclusion: The results indicate that Rh2 induces epigenetic methylation changes in genes involved in immune response and tumorigenesis, thereby contributing to enhanced immunogenicity and inhibiting the growth of cancer cells.

• IMMUNE NETWORK
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• v.2 no.1
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• pp.19-24
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• 2002

Background: Salvia miltiorrhiza (SM), a traditional oriental medicine, has been reported to have anti-tumor properties, but its exact mechanism remains to be elucidated. In this study, we investigated several of the molecular events that occur in human breast carcinoma MCF-7 cells and human pulmonary adenocarcinoma A549 cells. Methods: For this purpose, we evaluated the growth-inhibitory effect of SM in association with the expressions of p53, p21, cyclin D1, and pRb, which are known to be involved in cell cycle arrest. The extent of thymidine incorporation was also examined to assess G1/S phase cell cycle arrest in both cells by $^3H$-thymidine incorporation. Results: Our results show that SM inhibits the growth and the proliferation of MCF-7 and A549 cells. Furthermore, we also observed increased expression of p21 via a p53-dependent pathway in both cell lines after treating with SM. In addition, treatment with SM for 24 hours caused the suppression of hyperphosphorylated retinoblastoma protein (pRb) expression and the dephosphorylation of pRb. Conclusion: These findings suggest that the growth inhibitory and the anti-proliferation effects of SM on MCF-7 cells and A549 cells are mediated via the decreased expression and dephosphorylation of pRB by p21 up-regulation in a p53-dependent manner. To the best of our knowledge, this study is the first to report upon the molecular mechanisms involved in SM-induced tumor cell growth inhibition.

• IMMUNE NETWORK
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• v.13 no.2
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• pp.63-69
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• 2013

IL-12 is a secretory heterodimeric cytokine composed of p35 and p40 subunits. IL-12 p35 and p40 subunits are sometimes produced as monomers or homodimers. IL-12 is also produced as a membrane-bound form in some cases. In this study, we hypothesized that the membrane-bound form of IL-12 subunits may function as a costimulatory signal for selective activation of TAA-specific CTL through direct priming without involving antigen presenting cells and helper T cells. MethA fibrosarcoma cells were transfected with expression vectors of membrane-bound form of IL-12p35 (mbIL-12p35) or IL-12p40 subunit (mbIL-12p40) and were selected under G418-containing medium. The tumor cell clones were analyzed for the expression of mbIL-12p35 or p40 subunit and for their stimulatory effects on macrophages. The responsible T-cell subpopulation for antitumor activity of mbIL-12p35 expressing tumor clone was also analyzed in T cell subset-depleted mice. Expression of transfected membranebound form of IL-12 subunits was stable during more than 3 months of in vitro culture, and the chimeric molecules were not released into culture supernatants. Neither the mbIL-12p35-expressing tumor clones nor mbIL-12p40-expressing tumor clones activated macrophages to secrete TNF-${\alpha}$. Growth of mbIL-12p35-expressing tumor clones was more accelerated in the $CD8^+$ T cell-depleted mice than in $CD4^+$ T cell-depleted or normal mice. These results suggest that $CD8^+$ T cells could be responsible for the rejection of mbIL-12p35-expressing tumor clone, which may bypass activation of antigen presenting cells and $CD4^+$ helper T cells.

• IMMUNE NETWORK
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• v.11 no.6
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• pp.368-375
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• 2011

Background: Recent clinical observation reported that there was a significant correlation between change in circulating vascular endothelial growth factor (VEGF) levels and the occurrence of severe acute graft-versus-host disease (GVHD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT), but the action mechanisms of VEGF in GVHD have not been demonstrated. Methods: This study investigated whether or not blockade of VEGF has an effect on acute GVHD in a lethally irradiated murine allo-HSCT model of $B6\;(H-2^b)\;{\rightarrow}B6D2F1\;(H-2^{b/d})$. Syngeneic or allogeneic recipient mice were injected subcutaneously with anti-VEGF peptides, dRK6 ($50{\mu}g/dose$) or control diluent every other day for 2 weeks (total 7 doses). Results: Administration of the dRK6 peptide after allo-HSCT significantly reduced survival with greaterclinical GVHD scores and body weight loss. Allogeneic recipients injected with the dRK6 peptide exhibited significantly increased circulating levels of VEGF and expansion of donor $CD3^+$ T cells on day +7 compared to control treated animals. The donor $CD4^+$ and $CD8^+$ T-cell subsets have differential expansion caused by the dRK6 injection. The circulating VEGF levels were reduced on day +14 regardless of blockade of VEGF. Conclusion: Together these findings demonstrate that the allo-reactive responses after allo-HSCT are exaggerated by the blockade of VEGF. VEGF seems to be consumed during the progression of acute GVHD in this murine allo-HSCT model.