• The Plant Pathology Journal
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• v.39 no.1
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• pp.141-148
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• 2023

Black shoot blight disease caused by Erwinia pyrifoliae has serious impacts on quality and yield in pear production in Korea; therefore, rapid and accurate methods for its detection are needed. However, traditional detection methods require a great deal of time and fail to achieve absolute quantification. In the present study, we developed a droplet digital polymerase chain reaction (ddPCR) method for the detection and absolute quantification of E. pyrifoliae using a pair of species-specific primers. The detection range was 10³-10⁷ copies/ml (DNA templates) and cfu/ml (cell culture templates). This new method exhibited good linearity and repeatability and was validated by absolute quantification of E. pyrifoliae DNA copies from samples of artificially inoculated immature pear fruits. Here, we present the first study of ddPCR assay for the detection and quantification of E. pyrifoliae. This method has potential applications in epidemiology and for the early prediction of black shoot blight outbreaks.

• BMB Reports
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• v.33 no.2
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• Korean Journal of Digital Imaging in Medicine
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• v.3 no.1
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• pp.126-132
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• 1997

Purpose : To apply to Program of Auto processor quality control after comparison of Film density variations with amendments to Auto density by using Check density program and Adjust density program of calibration mode into the Laser imager. Methods : Observe Check and Adjust density variations on the Control chart with standard step and value during seven months from December, 1995 to June, 1956 extending twice a week. (1) Measure density value on the steps after printing out 17-step sensitometric pattern of the Check density program. (2) In the same way, measure density values after amending density by using Adjust density program If they are exceeding allowable error limit. Results : In case of Check density program, the exceeding limit rates of Density difference(DD) and Middle density(MD) are: FL-IM3543 DD=75%. MD=72.5%, FL-IMD DD=0%. MD=30.8%(14.5%) After amending density by using Adjust density program, the exceeding limit rates of all both Laser imager were zero percent. The standard deviations are show lower FL-IM D than FL-IM3543 on the Check density control chart, but higher on the Adjust density control chart. Conclusion : (1) Check density variations by printingout sensitometric pattern extending once a week at least for quality control of the Laser imager. (2) In case of a dusty place, check the Laser beam transmission after cleaning Laser optical unit extending once a month. (3) Be sure to measure and check density values by using adjust density program if they are exceeding allowable error limit. (4) Maintain much better film density by performing the adjust density program even if check density values are existed within normal limit.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.7
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• pp.741-749
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• 2003

In this paper, we investigated the degree of compensation for WDM channel signal distortion due to chromatic dispersion, self phase modulation(SPM) and cross phase modulation(XPM). The considered system is 120 Gbps (3${\times}$40 Gbps) intensity modulation direct detection(IM/DD) WDM transmission system with path-averaged intensity approximation(PAIA) mid-span spectral inversion(MSSI) as compensation method. This system have highly nonlinear dispersion shifted fiber(HNL-DSF) as nonlinear medium in optical phase conjugator(OPC). We use 1 dB eye opening peralty(EOP) in order to evaluate the characteristics of compensation for distorted WDM channels. We confirmed that improvement of transmission distance and performance is achieved by MSSI method to distorted long-haul IM/DD WDM channels due to chromatic dispersion, SPM and XPM. And in the aspect of compensation for distorted pulse due to XPM, the MSSI method is effective to IM/DD WDM transmission system with high fiber dispersion coefficient.

• Journal of Nutrition and Health
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• v.41 no.4
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• pp.291-298
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• 2008

To study antioxidant role of zinc, the effects of dietary zinc deficiency and vitamin E supplementation on lipid peroxidation were studied. Levels of zinc and vitamin E in blood and liver were also measured. Forty Sprague-Dawley male rats aging 8 weeks old were used as experimental animals. Zinc deficient diet (Zn, 0 ppm), zinc normal diet (Zn,36.5 ppm), and vitamin E supplemented diet (1,000 IU ${\alpha}$-tocopherol/kg of diet) were used as experimental diet. During the first three weeks, rats were divided into zinc normal (ZnN, 8 animals) and zinc deficient (ZnD, 32 animals) group. Eight rats from each group were sacrificed to get blood and liver after 3 weeks of experiment. The remaining 24 zinc deficient rat were then divided into zinc normal (ZnDN), zinc deficient (ZnDD), vitamin E supplemented (ZnDE) diet groups. After another 3 weeks of experiment, all animals were sacrificed as well. Thiobarbituric acid reactive substanc (TBARS) levels in plasma and liver, conjugated diene levels in liver were measured as lipid peroxidation index. There were no significant differences in food intake, body weight gain, and food efficiency ratio among groups. Weights of liver per 100 g body weight were not significantly different. There were no significant differences in Zn levels in serum. Plasma and liver TBARS level, and liver conjugated diene level were significantly lower in ZnDE than in ZnDN or ZnDD, and significantly higher in ZnDD than in ZnDN. Therefore, it seems that lipid peroxidation is accelerated by dietary zinc deficiency and recovered partly by vitamin E supplementation.

• The Korean Journal of Pain
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• v.35 no.1
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• pp.86-96
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• 2022

Background: To explore the association between low back pain (LBP) and pelvic pain (PP) and rectus abdominis diastasis (RAD) in postpartum women and identify the characteristics and risk factors. Methods: Women diagnosed with RAD and a history of labor and delivery, between 2009 and 2018, were identified from six hospitals within the Partners Healthcare System. Univariate and multivariable binary logistic regression analyses were used to identify the risk factors associated with pain. Results: Age at onset of RAD in the non-cesarean delivery group was earlier than those in cesarean delivery (CD) group (P = 0.017). Women who underwent CD demonstrated 4.5 times greater risk of RAD than those who had no CD exposure. The cumulative composition ratio of LBP at every age stage of the period from 8 years pre-first delivery to 8 years post-first delivery was significantly higher than the other five conditions (RAD, umbilical hernia, PP, depressive disorder [DD], and strain of muscle, fascia, and tendon [SMFT]) (P for trend < 0.001). Women with DD, SMFT, and PP were more likely to have LBP (odds ratio [OR] = 1.91, 95% confidence interval [CI] 1.06 to 3.47, P = 0.032; OR = 4.50, 95% CI 1.64 to 12.36, P = 0.003; OR = 2.14, 95% CI 1.17 to 3.89, P = 0.013; respectively). Conclusions: In postpartum women with RAD, DD, SMFT, and PP were found to be risk factors contributing to the development of LBP. Race and LBP also played roles in the development of PP.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.488-500
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• 2012

The present study was carried out to evaluate arthropod predators (Araneae, Carabidae, Staphylinidae, and Formicidae) as bioindicator for forest disturbance. We selected 12 study sites according to disturbance intensities caused by fire intensity and recovery methods at Goseong, Gangneung, and Samcheok in Gangwondo. Intensities of disturbance were characterized as follows: 0 (no fire), 1 (surface fire), 2 (crown fire), and 3 (crown fire followed by reforestation). Arthropod predators were captured using pitfall trap. Species richness and abundance of Araneae, Carabidae, Staphylinidae, and Formicidae were not different along disturbance intensities. However, species richness and abundance of grassland ants significantly increased in higher disturbance intensities. Abundance of grassland ants such as Formica japonica and Camponotus japonicas linearly decreased along litter depth. Furthermore, ant communities were clustered along disturbance intensities. In a one-time survey, ants may be most useful as bioindicator for forest disturbance among the four predator taxa, because ants actively forage for long seasons.

• Journal of radiological science and technology
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• v.46 no.3
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• pp.231-238
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• 2023

This study aimed to compare the results of delivery quality assurance (DQA) using MapCHECK and OCTAVIUS for radiation therapy. Thirty patients who passed the DQA results were retrospectively included in this study. The point dose difference (DD) and gamma passing rate (GPR) were analyzed to evaluate the agreement between the measured and planned data for all cases, Plan complexity was evaluated to analyze dosimetric accuracy by quantifying the degree of modulation according to each plan. We analyzed the monitor units (MUs) and total MUs for each plan to evaluate the correlation between the MUs and plan complexity. We used a paired t-test to compare the DD and GPRs that were obtained using the two devices. The DDs and GPRs were within the tolerance range for all cases. The average GPRs difference between the two devices was statistically significant for the brain, and head and neck for gamma criteria of 3%/3 mm and 2%/2 mm. There was no significant correlation between the modulation index and total MUs for any of the cases. These DQA devices can be used interchangeably for routine patient-specific QA in radiation therapy.

• 전기산업
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• v.7 no.2
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• pp.98-106
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• 1996

• Journal of the KSME
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• v.31 no.1
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• pp.43-50
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• 1991

로봇의 설계시 고려하여야 하는 여러가지 면을 기술하였다. 평면 3자유도 링크의 경우 길이비를 "Fibonacci Sequence"를 따라가도록 결정하였으며 여유자유도를 갖는 직접구동로봇의 설계 및 제작시 유의하여야 할 사항을 정리하였다. 몸체설계후 POSTECH DD 로봇을 위한 실시간 제 어환경이 구성되었으며 VME버스의 MC68020/68882 프로세서를 탑재한 "one board" 컴퓨터에서 모든 로봇제어 소프트웨어가 수행되었다.든 로봇제어 소프트웨어가 수행되었다.