• International Journal of Oral Biology
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• v.34 no.1
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• pp.37-42
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• 2009

The attachment and adhesion of RAW 264.7 and MC3T3-E1 cells to titanium (Ti) discs with various degrees of roughness was investigated. The attachment, adhesion, and proliferation of these cells were evaluated after 4 hr, 24 hr and 7 day incubations. Both RAW 264.7 and MC3T3-E1 cells showed a time-dependant correlation between attachment and adhesion on the surface of the titanium discs. Both types of cells tended to have higher survival rate on these discs as the surface roughness increased. The percentage of adherent inflammatory RAW 264.7 cells was greater than MC3T3-E1 cells at 24 hr, but this was reversed at 7 days in culture. The morphology of osteoblastic MC3T3-E1 cells at 24 hr, determined using a surface emission microscope (SEM), appeared flattened and spread out while inflammatory RAW 264.7 cells were predominantly spherical in shape. The adhesion of both cell types on the titanium discs was dependant on the levels of fibronectin adsorbed on the disc surface, indicating that serum constituents modulate the efficient adhesion of these cells. Our data indicate that the cellular response to the titanium surface is dependent on the types of cells, surface roughness and serum constituents.

• Korean Journal of Ecology and Environment
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• v.47 no.4
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• pp.312-318
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• 2014

This study was conducted to establish models of filtrating rate and production of feces of a native freshwater bivalve, Anodonta woodiana, on removal organic matters in eutrophic waters. Among the applied shell size (4.3~15.5 cm), the filtrating rate and production of feces of Anodonta woodiana was $0.08{\sim}0.86L\;g^{-1}\;h^{-1}$ (average $0.24L\;g^{-1}\;h^{-1}$), $0.00{\sim}11.10mg\;g^{-1}\;h^{-1}$ (average $0.94mg\;g^{-1}\;h^{-1}$), respectively. In two different water current (high $48Lh^{-1}$, low $24Lh^{-1}$), the filtrating rate of Chl-a was $0.02{\sim}0.10L\;g^{-1}\;d^{-1}$ (average $0.05L\;g^{-1}\;d^{-1}$), $0.02{\sim}0.11L\;g^{-1}\;d^{-1}$ (average $0.07L\;g^{-1}\;d^{-1}$) and the removal rate was 65.4%, 82.1%, respectively. Response surface methodology, with a central composite design comprising 3 levels and 2 variables, was used to identify the optimal removal condition of shell length, water current and filtrating rate or feces production by bivalves. The optimum removal conditions were found that had optimized $6.21L\;mussel^{-1}\;d^{-1}$ at shell length 14.3~15.6 and water current $22{\sim}30Lh^{-1}$. The optimal conditions of production of feces ($4.2mg\;g^{-1}\;d^{-1}$) by freshwater mussels were shell length 14.3~16.3 cm and water current $36{\sim}44Lh^{-1}$.

• Design & Manufacturing
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• v.17 no.3
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• pp.1-7
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• 2023

Blow molding is a manufacturing process in which thermoplastic preforms are preheated and then pneumatically expanded within a mold to produce hollow products of various shapes. The two-step process, a type of blow molding method, requires the output of multiple infrared lamps to be adjusted individually, so the process of finding initial conditions hinders productivity. In this study, digital twin technology was applied to solve this problem. A blow molding simulation technique was established and simulation-based metadata was generated. A response surface ROM (Reduced Order Model) was built using the generated metadata. Then, a dynamic ROM was constructed using the results of 3D heat transfer analysis. Through this, users can quickly check the product wall thickness uniformity according to changes in the control value of the heating lamp for products of various shapes, and at the same time, check the temperature distribution of the preform in real time.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.84-87
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• 2001

The high speed machining which cam improve the production and quality has been remarkable in die/mold industry with the growth of parts and materials industries. The speed of machine tool increases, but on the other hand, the response of sensors I not being improved. Therefore, the condition monitoring techniques for the machine too, tool and workpiece in high speed machining are incomplete. In this study, characteristics of the tool edge roughness were verified from the high frequency components of cutting force signals acquired by the high speed dynamometer. Also, the experimental evaluation technique for the machinability and condition monitoring in high speed machining was established by analyzing the cutting force, acceleration and surface roughness.

• Journal of Power Electronics
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• v.16 no.3
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• pp.1004-1011
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• 2016

The inaccurate model parameters in the predictive current control of surface-mounted permanent magnet synchronous motor (SMPMSM) affect the current dynamic response and steady-state error. This paper presents a model parameter correction algorithm based on the relationship between the errors of model parameters and the static errors of dq-axis current. In this correction algorithm, the errors of inductance and flux are corrected in two steps. Resistance is ignored. First, the proportional relations between inductance and d-axis static current errors are utilized to correct the error of model inductance. Second, the flux is corrected by utilizing the proportional relations between flux and q-axis static current errors under the condition that inductance is corrected. An experimental study with a 100 W SMPMSM is performed to validate the proposed algorithm.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.29 no.5
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• pp.377-388
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• 2016

This study is aimed to evaluate the embedment effect of foundation as compared to the surface foundation on the response of a base-isolated nuclear power plant structure. For this purpose, the boundary reaction method (BRM), which is a two-step frequency domain and time domain technique, is used for the nonlinear SSI analysis considering nonlinear behavior of base isolators. The numerical model of the BRM is verified by comparing the numerical results obtained by the BRM and the conventional frequency-domain SSI analysis for an equivalent linear SSI system. Finally, the displacement response of the base isolation and the horizontal response of the structure obtained by the nonlinear SSI analysis using the moat foundation model are compared with those using the surface foundation model. The comparison showed that the displacement response of the base isolation can be reduced by considering the embedment effect of foundation.

• Fisheries and Aquatic Sciences
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• v.25 no.3
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• pp.175-186
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• 2022

Sea mustard (Undaria pinnatifida), an important edible seaweed belonging to the brown algal family of Alariaceae, contains copious physiologically active substances. It has long been popular in Korea as a food and is frequently consumed in the form of soup. It is also commercially available as a home meal replacement. In this study, we developed a seasoning key base with a high degree of sensory preference from sea mustard using the extrusion cooking process. Extrusion cooking conditions were optimized through response surface methodology. Barrel temperature (X₁, 140℃-160℃) and screw speed (X₂, 158-315 rpm) were set as independent variables, and overall preference was determined as the dependent variable (Y, points). An optimal condition was obtained at X₁ = 148.5℃ and X₂ = 315 rpm, and the dependent variable (Y, overall acceptance) was 7.95 points, similar to the experimental value of 7.81. Umami taste had a relationship with the overall acceptance of sea mustard seasoning. In the electronic nose and tongue, increased sourness and umami intensities were associated with the highest sensory score. The samples were separated well by each characteristic via principal component analysis. Collectively, our study provides imperative preliminary data for the development of various seasonings using sea mustard.

• Molecules and Cells
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• v.24 no.1
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• pp.119-124
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• 2007

TLR4 together with CD14 and MD-2 forms a pattern recognition receptor that plays an initiating role in the innate immune response to Gram-negative bacteria. Here, we employed the surface plasmon resonance technique to investigate the kinetics of binding of LPS to recombinant CD14, MD-2 and TLR4 proteins produced in insect cells. The dissociation constants ($K_D$) of LPS for immobilized CD14 and MD-2 were $8.7{\mu}m$, and $2.3{\mu}m$, respectively. The association rate constant ($K_{on}$) of LPS for MD-2 was $5.61{\times}10^3M^{-1}S^{-1}$, and the dissociation rate constant ($K_{off}$) was $1.28{\times}10^2S^{-1}$, revealing slow association and fast dissociation with an affinity constant $K_D$ of $2.33{\times}10^6M$ at $25^{\circ}C$. These affinities are consistent with the current view that CD14 conveys LPS to the TLR4/MD-2 complex.

• Applied Chemistry for Engineering
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• v.29 no.5
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• pp.503-509
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• 2018

In this study, we used the ultrasonic extraction process as a method to extract antioxidant substances from kudzu, and measured the content of iso-flavonoids puerarin, daidzein, daidzin contained in kudzu. The response surface methodology which is a statistical analysis method for optimizing the extraction amount of iso-flavonoids from the kudzu and the process condition for maximizing the yield was applied. It is the final objective of this study to effectively derive the condition of the process that matches the target response with a minimum number of experiments and analyze the effect of each process condition on the response. In the response surface methodology, the central composite design was applied and the optimum condition was analyzed, and the three independent variables were set to ultrasonic irradiation time, volume ratio of ethanol/ultrapure water, ultrasonic irradiation power. Using the response surface methodology, the optimum conditions with the maximum extraction yield and the content of iso-flavonoids were evaluated as ultrasonic irradiation time (24.75 min), ethanol / ultrapure water volume ratio (39.75 vol%), ultrasonic irradiation power (592.36 W). The overall satisfaction level appears as high as 0.8938, which is recognized at a significance level within 5%. As a result of analyzing the optimization process, it was confirmed that the ultrasonic irradiation time is the factor that most affects the responses.

• 한국신재생에너지학회:학술대회논문집
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• 2009.06a
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• pp.600-603
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• 2009

We compared relative importance of thermal conductivity and initial ground temperature in designing borehole heat exchanger network and also we test accuracy of ground temperature estimation in thermal response test using a proven 3-D T-H modeler. The effect of error in estimating ground temperature on calculated total length of borehole heat exchanger was more than 3 times larger than the case of thermal conductivity in maximum 20% error range. Considering 10% of error in estimating thermal conductivity is generally acceptable, we have to define the initial ground temperature within 5% confidence level. Utilizing the mean annual ground surface temperature and the geothermal gradient map compiled so far can be a economic way of estimating ground temperature with some caution. When performing thermal response test for estimating ground temperature as well as measuring thermal conductivity, minimum 100 minutes of ambient circulation is required, which should be even more in case of very cold and hot seasons.