• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.28-33
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• 1998

A gene coding for ${\beta}$-xylosidase from thermophilic xylanolytic Bacillus sp. KK-1 was cloned into Escherichia coli using plasmid pBR322. Recombinant plasmid DNAs were isloated from E. coli clones which were capable of hydrolyzing 4-methylumbelliferyl-${\beta}$-D xylopyranoside. Restriction analysis showed the DNAs to share a common insert DNA. Xylo-oligosaccharides, including xylotriose, xylotetraose, xylopentaose, and xylobiose were hydrolyzed to form xylose as an end product by cell-free extracts of the E. coli clones, confirming that the cloned gene from strain KK-1 is ${\beta}$-xylosidase gene. The ${\beta}$-xylosidase gene of strain KK-1 designated as xylB was completely sequenced. The xylB gene consisted of an open reading frame of 1,602 nucleotides encoding a polypeptide of 533 amino acid residues, and a TGA stop codon. The 3' flanking region contained one stem-loop structure which may be involved in transcriptional termination. The deduced amino acid sequence of the KK-1 ${\beta}$-xylosidase was highly homologous to the ${\beta}$-xylosidases of Bacillus subtilis and Bacillus pumilus, but it showed no similarity to a thermostable ${\beta}$-xylosidase from Bacillus stearothermophilus.

• Earthquakes and Structures
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• v.7 no.1
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• pp.57-82
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• 2014

The heritage value of the mixed wood-masonry 18th century Pombalino buildings of downtown Lisbon is recognized both nationally and internationally. The present paper focuses on the seismic assessment of global response and retrofitting of a typical Pombalino building by nonlinear static analyses, performed by the research software Tremuri, which is able to model 3D configurations. The structure is modelled using nonlinear beams for masonry panels, while in case of the internal walls (frontal walls) an original formulation has been developed in order to take into account their specific seismic behaviour. Floors are modelled as orthotropic membrane finite elements: this feature allows to simulate the presence of both flexible and rigid diaphragms, being the first ones more representative of the original state while the second ones of retrofitted configurations. Seismic assessment has been evaluated by applying nonlinear static procedure and comparing the performance of different configurations (by considering various retrofitting strategies). Finally, assuming a lognormal cumulative distribution, fragility curves are obtained to be representative of Pombalino buildings: the most important application of such curves is for seismic risk and loss estimation analyses.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.20 no.9
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• pp.42-47
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• 2021

A one-touch descending lifeline that can easily be installed and rapidly evacuated in the event of a fire accident in high-rise buildings was proposed to overcome difficulties of conventional descending lifeline such as complex installation methods and procedures. However, this lifeline exhibits limitations such as restrictions in installation location and large apparatus size. Therefore, this paper proposes a sliding-type descending lifeline, which has a similar operation to that of current one-touch descending lifeline and solves the aforementioned limitations. A double square link mechanism including a sliding four-bar linkage is proposed and the descending lifeline support is redesigned to unfold in two different planes, allowing 3D movement. Additionally, the shape of the support frame is designed to obtain two attachment surfaces that can be attached to a wall, irrespective of the angle between the window and the inner wall. FEA analysis using ABAQUS is performed to ensure that the robustness of the presented support complies with the Fire Control Act Enforcement Decree. Finally, the feasibility of the proposed sliding one-touch descending lifeline is verified through fabrication.

• Earthquakes and Structures
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• v.12 no.1
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• pp.1-12
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• 2017

Historical earthquakes have shown that successive seismic events may occur in regions of high seismicity. Such a sequence of earthquakes has the potential to increase the damage level of the structures, since any rehabilitation between the successive ground motions is practically impossible due to lack of time. Few studies about this issue can be found in literature, most of which focused their attention on the seismic response of SDOF systems or planar frame structures. The aim of the present study is to examine the impact of seismic sequences on the damage level of 3D multistorey R/C buildings with various structural systems. For the purposes of the above investigation a comprehensive assessment is conducted using three double-symmetric and three asymmetric in plan medium-rise R/C buildings, which are designed on the basis of the current seismic codes. The buildings are analyzed by nonlinear time response analysis using 80 bidirectional seismic sequences. In order to account for the variable orientation of the seismic motion, the two horizontal accelerograms of each earthquake record are applied along horizontal orthogonal axes forming 12 different angles with the structural axes. The assessment of the results revealed that successive ground motions can lead to significant increase of the structural damage compared to the damage caused by the corresponding single seismic events. Furthermore, the incident angle can radically alter the successive earthquake phenomenon depending on the special characteristics of the structure, the number of the sequential earthquakes, as well as the distance of the record from the fault.

• The korean journal of orthodontics
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• v.53 no.4
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• pp.254-263
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• 2023

Objective: The evidence on the accuracy of bite registration using intraoral scanners is sparse. This study aimed to develop a new method for evaluating bite registration accuracy using intraoral scanners. Methods: Two different types of models were used; 10 stone models and 10 with acrylic resin teeth. A triangular frame with cylindrical posts at each apex (one anterior and two posteriors) was digitally designed and manufactured using three-dimensional (3D) printing. Such a structure was fitted in the lingual space of each maxillary and mandibular model so that, in occlusion, the posts would contact their opposing counterparts, enforcing a small interocclusal gap between the two arches. This ensured no tooth interference and full contact between opposing posts. Bite registration accuracy was evaluated by measuring the distance between opposing posts, with small values indicating high-accuracy. Three intraoral scanners were used: Medit i500, Primescan, and Trios 4. Viewbox software was used to measure the distance between opposing posts and compute roll and pitch. Results: The average maximum error in interocclusal registration exceeded 50 ㎛. Roll and pitch orientation errors ranged above 0.1 degrees, implying an additional interocclusal error of around 40 ㎛ or more. The models with acrylic teeth exhibited higher errors. Conclusions: A method that avoids the need for reference hardware and the imprecision of locating reference points on tooth surfaces, and offers simplicity in the assessment of bite registration with an intraoral scanner, was developed. These results suggest that intraoral scanners may exhibit clinically significant errors in reproducing the interocclusal relationships.

• Journal of Korean Society of Steel Construction
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• v.18 no.3
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• pp.385-393
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• 2006

In Korea, the effective utilization of wod structure is encour aged to preserve natural resources and the global environment.ote demand for wod. The efective combination of structural la minated timber and other materials is expected to extend the potential of building structures. This research examines the moment resis tance-type jointing method using structural laminated timber and H-section stel aiming at development of the two-direction frame for lar ge 9 mm and 12 mm) of the H section. Therefore, we conducted the experiment with bending test of the joints to investigate the s tifnes, strength, strain distributions of laminated timber an d of the flange of the H section, and failure paterns. As shown in the results, t he joints with a flange thicknes of 9 mm and 12 m have superi or strength with a flange thicknes of 9 mm and 12 mm were very large, whic h confirmed the high level of energy absorption of such structure s.

• International journal of steel structures
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• v.18 no.5
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• pp.1617-1630
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• 2018

The crucial differences between conventional rail with split-type connectors and continuous welded rails are axial stress in the longitudinal direction and stability, as well as other issues generated under the influence of loading effects. Longitudinal stresses generated in continuously welded rails on railway bridges are strongly influenced by the nonlinear behavior of the supporting system comprising sleepers and ballasts. Thus, the track structure interaction cannot be neglected. The rail-support system mentioned above has properties of non-uniform material distribution and uncertainty of construction quality. The linear elastic hypothesis therefore cannot correctly evaluate the stress distribution within the rails. The aim of this study is to apply the nonlinear finite element method using the nonlinear coupling interface between the track and structural model and to illustrate the welded rail behavior under the loading effect and uncertain factors of the ballast. Numerical results of nonlinear finite analysis with a three-dimensional solid and frame element model are presented for a typical track-bridge system. A composite plate girder, modeled by solid and shell elements, is also analyzed to consider the behavior of the welded rail. The analysis result showed buckling under the independent calculations of load cases, including 'temperature change', 'bending of the supporting structure', and 'braking' of the railway vehicle. A parametric study of the load combination method and the loading sequence is also included in this analysis.

• Radiation Oncology Journal
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• v.17 no.1
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• pp.84-88
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• 1999

Purpose :To develop a method for verifying a treatment setup in stereotactic radiotherapy by ma- tching portal images to DRRs. Materials and Methods : Four pairs of orthogonal portal images of one patient immobilized by a thermoplastic mask frame for fractionated stereotactic radiotherapy were compared with DRRs. Portal images are obtained in AP (anteriorfposterior) and lateral directions with a target localizer box containing fiducial markers attached to a stereotactic frame. DRRs superimposed over a planned iso-center and fiducial markers are printed out on transparent films. And then, they were overlaid over onhogonal penal images by matching anatomical structures. From three different kind of objects (isgcenter, fiducial markers, anatomical structure) on DRRs and portal images, the displacement error between anatomical structure and isocenters (overall setup error), the displacement error between anatomical structure and fiducial markers (irnrnobiliBation error), and the displacement error between fiducial markers and isocenters (localization error) were measured. Results : Localization error were 1.5$\pm$0.3 mm (AP), 0.9$\pm$0.3 mm (lateral), and immobilization errors were 1.9$\pm$0.5 mm (AP), 1.9$\pm$0.4 mm (lateral). In addition, overall setup errors were 1.0$\pm$0.9 mm (AP), 1.3$\pm$0.4 mm (lateral). From these orthogonal displacement errors, maximum 3D displacement errors($\sqrt{(\DeltaAP)^{2}+(\DeltaLat)^{2}$)) were found to be 1.7$\pm$0.4 mm for localization, 2.0$\pm$0.6 mm for immobilization, and 2.3$\pm$0.7 mm for overall treatment setup. Conclusion : By comparing orthogonal portal images with DRRs, we find out that it is possible to verify treatment setup directly in stereotactic radiotherapy.

• Journal of Korean Society of Steel Construction
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• v.29 no.5
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• pp.361-368
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• 2017

ASCE 7-10 defines the torsional irregular structure as the one that has large torsional responses caused by the eccentricity. The code requires that these structures should be designed abide by the torsional provisions. This study evaluates the influence of torsional provisions on the performance of the designed multiple steel moment frames with different eccentricity. In this study, 3D response history analyses are performed. The results show that the moment frame design according to the standard with torsional irregularity provisions showed larger performance as the eccentricity increased and the distribution of plastic hinges similarly to orthopedic structures.

• Journal of Digital Contents Society
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• v.18 no.3
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• pp.577-584
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• 2017

Despite growing participation of many researchers, the cases of applying the transformative emotional display technology using physical materials and media are still in its early stages and the concept and structure are not properly established in Korea. The emerging global trend of physical display shows a possibility of new digital signage using emotional flip display. In this context, this study attempts to develop related element technology necessary in physical flip display and electromagnetic rotation module along with the integration platform of aesthetic arbitrarily-shaped display. The found results will be applied in researching the contents for the main subject which will be exhibited at the EXPO 2017 in Astana. It is to build up the foundation pioneering the localization of associated industry by means of diverse technological breakthroughs including flip-display technology, electromagnetic module technology, real-time video inter-working technology, high-speed rotation module technology, and transformative 3D frame technology.