• 제목/요약/키워드: 3D digital microscope

검색결과 43건 처리시간 0.028초

미세유체소자와 디지털 홀로그래피 기술을 이용한 미생물의 3D 이미징과 세그먼테이션 (3D sensing and segmentation of microorganism using microfluidic device and digital holography)

  • 신동학;이준재
    • 한국정보통신학회논문지
    • /
    • 제17권2호
    • /
    • pp.447-452
    • /
    • 2013
  • 미세유체소자(microfluidic device)는 미생물과 관련된 다양한 작업들에 대해서 정확한 제어를 제공할 수 있다. 본 논문에서는 미세유체 소자와 디지털 홀로그래피 마이크로스코피 기술로 구성된 시스템을 구성하고 미생물의 3D 이미징과 세그먼테이션을 설명한다. 각각의 미생물은 미세유체 채널을 통하여 흘러가며 홀로그래피 마이크로스코피가 홀로그램을 기록한다. 기록된 홀로그램은 Fresnel 변환을 통하여 컴퓨터적으로 복원되며, 복원된 영상의 위상성분을 이용하여 미생물의 위치 정보를 찾기 위한 세그먼테이션을 수행한다. 제안하는 방법의 유용함을 설명하기 위하여 광학 실험을 수행하고 그 결과를 나타내었다.

Lymphovenous anastomoses with three-dimensional digital hybrid visualization: improving ergonomics for supermicrosurgery in lymphedema

  • Will, Patrick A.;Hirche, Christoph;Berner, Juan Enrique;Kneser, Ulrich;Gazyakan, Emre
    • Archives of Plastic Surgery
    • /
    • 제48권4호
    • /
    • pp.427-432
    • /
    • 2021
  • The conventional approach of looking down a microscope to perform microsurgical procedures is associated with occupational injuries, anti-ergonomic postures, and increased tremor and fatigue, all of which predispose microsurgeons to early retirement. Recently, three-dimensional (3D) visualization of real-time microscope magnification has been developed as an alternative. Despite its commercial availability, no supermicrosurgical procedures have been reported using this technology to date. Lymphovenous anastomoses (LVAs) often require suturing vessels with diameters of 0.2-0.8 mm, thus representing the ultimate microsurgical challenge. After performing the first documented LVA procedure using 3D-augmented visualization in our unit and gaining experience with this technique, we conducted an anonymized in-house survey among microsurgeons who had used this approach. The participants considered that 3D visualization for supermicrosurgery was equivalent in terms of handling, optical detail, depth resolution, and safety to conventional binocular magnification. This survey revealed that team communication, resident education, and ergonomics were superior using 3D digital hybrid visualization. Postoperative muscle fatigue, tremor, and pain were also reduced. The major drawbacks of the 3D visualization microscopic systems are the associated costs, required space, and difficulty of visualizing the lymphatic contrast used.

3D 디지털 현미경으로 측정한 당백가시 인쇄본의 형상 특징 (An Image Characteristics of Metal Movable Type Printing on One Hundred Poets of the Tang Dynasty by the Measurement of 3D Digital Microscope)

  • 김혜경;오카다 요시히로
    • 보존과학회지
    • /
    • 제33권1호
    • /
    • pp.43-49
    • /
    • 2017
  • 류코쿠 대학 소장 "당백가시"를 중심으로 3D 디지털 현미경으로 인쇄된 종이 표면에 남아있는 금속활자의 미세표면을 관찰하여 굴곡구조와 표면 거칠기를 정량화하는 측정 방법을 소개하고자 한다. 인쇄된 종이 표면을 기준으로 활자 표면의 굴곡 현상을 선으로 측정하고 활자의 미세표면은 면으로 측정하여 수치화하여 인쇄된 종이 표면의 특성을 제시하였다. 3D 디지털 현미경 측정 방법은 비접촉, 비파괴 방법으로 많은 자료 분석과 반사광에 의한 직접 관찰이 가능하다. 그리고 클리닝이나 배접 그리고 주름을 펴기 위하여 강한 힘으로 압력을 주는 과정에서 고문서에 함유된 정보가 손실될 수 있으므로 사전 조사 방법으로 종이 표면에 남아있는 인쇄 정보를 수집하는데 유용한 방법이라고 할 수 있다.

Application of Three-dimensional Scanning, Haptic Modeling, and Printing Technologies for Restoring Damaged Artifacts

  • Jo, Young Hoon;Hong, Seonghyuk
    • 보존과학회지
    • /
    • 제35권1호
    • /
    • pp.71-80
    • /
    • 2019
  • This study examined the applicability of digital technologies based on three-dimensional(3D) scanning, modeling, and printing to the restoration of damaged artifacts. First, 3D close-range scanning was utilized to make a high-resolution polygon mesh model of a roof-end tile with a missing part, and a 3D virtual restoration of the missing part was conducted using a haptic interface. Furthermore, the virtual restoration model was printed out with a 3D printer using the material extrusion method and a PLA filament. Then, the additive structure of the printed output with a scanning electron microscope was observed and its shape accuracy was analyzed through 3D deviation analysis. It was discovered that the 3D printing output of the missing part has high dimensional accuracy and layer thickness, thus fitting extremely well with the fracture surface of the original roof-end tile. The convergence of digital virtual restoration based on 3D scanning and 3D printing technology has helped in minimizing contact with the artifact and broadening the choice of restoration materials significantly. In the future, if the efficiency of the virtual restoration modeling process is improved and the material stability of the printed output for the purpose of restoration is sufficiently verified, the usability of 3D digital technologies in cultural heritage restoration will increase.

Improving Phase Contrast of Digital Holographic Microscope using Spatial Light Modulator

  • Le, Thanh Bang;Piao, Meilan;Jeong, Jong-Rae;Jeon, Seok-Hee;Kim, Nam
    • Journal of the Optical Society of Korea
    • /
    • 제19권1호
    • /
    • pp.22-28
    • /
    • 2015
  • We propose a new method for improving the phase contrast of a multiphase digital holographic microscope using a spatial light modulator (SLM). Using the SLM as the annulus, our method improves the light contrast of the object edge to achieve higher accuracy. We demonstrate a digital holographic microscopy technique that provides a 30% improvement in the phase contrast compared to conventional microscopy, which utilizes a mechanical annulus. The phase-contrast improvement allows the 3D reconstructed hologram to be determined more precisely.

From Exoscope into the Next Generation

  • Nishiyama, Kenichi
    • Journal of Korean Neurosurgical Society
    • /
    • 제60권3호
    • /
    • pp.289-293
    • /
    • 2017
  • An exoscope, high-definition video telescope operating monitor system to perform microsurgery has recently been proposed an alternative to the operating microscope. It enables surgeons to complete the operation assistance by visualizing magnified images on a display. The strong points of exoscope are the wide field of view and deep focus. It minimized the need for repositioning and refocusing during the procedure. On the other hand, limitation of magnifying object was an emphasizing weak point. The procedures are performed under 2D motion images with a visual perception through dynamic cue and stereoscopically viewing corresponding to the motion parallax. Nevertheless, stereopsis is required to improve hand and eye coordination for high precision works. Consequently novel 3D high-definition operating scopes with various mechanical designs have been developed according to recent high-tech innovations in a digital surgical technology. It will set the stage for the next generation in digital image based neurosurgery.

SEM 영상의 자체검정에 의한 미세구조물의 3차원 표면모델 생성 (3D Surface Model Generation of Micro Structure by Self Calibration of The SEM Image)

  • 이효성;박형동
    • 한국측량학회:학술대회논문집
    • /
    • 한국측량학회 2003년도 춘계학술발표회 논문집
    • /
    • pp.151-159
    • /
    • 2003
  • This study presents method for self-calibration of the SEM(Scanning Electron Microscope) stereo image using the standard microprobe with same grid pattern and using parallel and central perspective projection equation. Result showed that parallel projection method is more suitable for standard microprobe. The maximum error of 3D coordinates acquired by this method did not exceed 5 $\mu\textrm{m}$, and DSM(Digital Surface Model) for three dimensional measurement of the rock sample was generated by the digital photogrammetry. This result can be used for quantification of micro scale change of shape and analysis of the micro morphology of rock due to weathering.

  • PDF

확장된 피사계 심도 알고리즘에서 엣지 정보 분석에 의한 3차원 깊이 정보 추출 방법 (The 3D Depth Extraction Method by Edge Information Analysis in Extended Depth of Focus Algorithm)

  • 강선우;김준식;주효남
    • 제어로봇시스템학회논문지
    • /
    • 제22권2호
    • /
    • pp.139-146
    • /
    • 2016
  • Recently, popularity of 3D technology has been growing significantly and it has many application parts in the various fields of industry. In order to overcome the limitations of 2D machine vision technologies based on 2D image, we need the 3D measurement technologies. There are many 3D measurement methods as such scanning probe microscope, phase shifting interferometry, confocal scanning microscope, white-light scanning interferometry, and so on. In this paper, we have used the extended depth of focus (EDF) algorithm among 3D measurement methods. The EDF algorithm is the method which extracts the 3D information from 2D images acquired by short range depth camera. In this paper, we propose the EDF algorithm using the edge informations of images and the average values of all pixel on z-axis to improve the performance of conventional method. To verify the performance of the proposed method, we use the various synthetic images made by point spread function(PSF) algorithm. We can correctly make a comparison between the performance of proposed method and conventional one because the depth information of these synthetic images was known. Through the experimental results, the PSNR of the proposed algorithm was improved about 1 ~ 30 dB than conventional method.

치과용 3D 프린팅 시스템에 의해 출력된 보철물의 품질 평가 (An evaluation of quality of dental prostheses printed by dental 3-dimensional printing system)

  • 한만소
    • 대한치과기공학회지
    • /
    • 제38권3호
    • /
    • pp.185-191
    • /
    • 2016
  • Purpose: The purpose of this study were to evaluate the quality of dental prostheses printed by 3-dimensional printing system. Methods: Mater model was prepared and ten study models were fabricated. Ten single crowns were printed by 3D-printing system(Resin group) and another ten single crowns using casting method were manufactured(Metal group). The marginal adaptation of single crowns were measured using by silicone replica technique. Silicone replicas were sectioned four times. The marginal adaptations were evaluated using by digital microscope. Statistical analyses were performed with Mann-Whitney test(${\alpha}=0.05$). Results: $Mean{\pm}standard$ deviations of all marginal adaptations were $92.1(20.0){\mu}m$ for Metal group and $69.7(12.3){\mu}m$ for Resin group. Two groups were no statistically significant differences(p>0.05). Conclusion: Marginal adaptation of single crowns printed by 3D-printing system were ranged within the clinical recommendation.