• 정보처리학회논문지B
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• 제11B권6호
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• pp.661-666
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• 2004

통신 기술의 발전으로 무선 채널을 이용한 멀티미디어 서비스를 제공하기 위한 방법이 활발히 연구되고 있다. 이에 따라 최근 무선망이나 인터넷과 같이 오류가 많이 발생하는 채널을 통한 비디오의 전송이 급격히 증가하면서 채널 상에서 발생하는 전송에러로 인한 데이터의 손실이 심각한 문제로 대두되고 있으며, 이에 따라 에러 은닉이 주된 관심사로 부각되었다. 에러 은닉 기법은 부가 정보가 필요 없으며, 부호화 기법의 변형 없이 적용이 가능하기 때문에 널리 연구되고 있다. 본 논문에서는, 손실된 블록 주위의 정상블록들 중 움직임 벡터들의 적응적 선택을 이용하여 중간 값을 취한 후, 손실된 블록의 새로운 움직임 벡터로 할당하고, OBMC(Overlapped Block Motion Compensation)을 통해 최종적으로 손실영역을 은닉하게 된다. 이 방법은 연속된 GOB(Group Of Block)의 손실에 있어서 효과적임을 알 수 있었다. 실험 결과 PSNR 성능평가 면에서 제안한 방법의 결과가 기존의 방법들 중 우수한 에러은닉 결과를 내는 MVRI(Motion Vector Rational Interpolation)이나 메디안$.$중첩 에러 은닉 기법에 비해 약 3db정도 향상된 결과를 얻을 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.376-380
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• 1999

Constructs, encoding a single-chain variable fragment of a catalytic antibody 4f4f (scFv-4f4f) with glycosidase activity, were made by combining the coding sequences for the heavy and light chain variable domains with a sequence encoding a linker (GGGGS). Using three different plasmid systems, single-chain antibodies were expressed separately in Escherichia coli, demonstrating significant differences in the expression level and amounts in soluble form of the recombinant protein. The protein expression from pET3a-scFv-4f4f was up to 20% of the total soluble proteins and, more importantly, the proteins were mostly found in a soluble form. An SDS-PAGE analysis of the purified single-chain proteins, yielding higher than 5mg from a 1-1 culture, showed a single band corresponding to its molecular weight of 29,100. A preliminary study shows that the expressed scFv-4f4f is catalytically active. The catalytic parameters for the hydrolysis of p-nitrophenyl-$\beta$-D-glucopyranoside by scFv-4f4f are being investigated.

• 대한인간공학회지
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• 제29권6호
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• pp.925-932
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• 2010

Most of the research on three-dimensional interaction field have showed different accuracy in terms of sensing, mode and method. Furthermore, implementation of interaction has been a lack of consistency in application field. Therefore, this study is to suggest research trends of three-dimensional interaction using meta-analysis. Searching relative keyword in database provided with 153 domestic papers and 188 international papers covering three-dimensional interaction. Analytical coding tables determined 18 domestic papers and 28 international papers for analysis. Frequency analysis was carried out on method of action, element, number, accuracy and then verified accuracy by effect size of the meta-analysis. As the results, the effect size of sensor-based was higher than vision-based, but the effect size was extracted to small as 0.02. The effect size of vision-based using hand motion was higher than sensor-based using hand motion. Therefore, implementation of three-dimensional sensor-based interaction and vision-based using hand motions more efficient. This study was significant to comprehensive analysis of three-dimensional motion recognition for interaction and suggest to application directions of three-dimensional interaction.

• 방송공학회논문지
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• 제9권4호
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• pp.446-454
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• 2004

멀티미디어 시스템이 발전함에 따라 멀티미디어 서비스 내에서 영상데이터 압축의 중요성은 점점 강조되고 있다. 그러나 영상을 압축한 비트스트림에 오류가 발생할 경우 영상을 복원할 때 심각한 왜곡이 발생하고, 이 때문에 멀티미디어 서비스에서 오류 은닉 방법은 더욱 중요한 기술로 대두되고 있다. 이러한 점을 해결하기 위해 Hsia는 1차원 경계면에서의 정합벡터를 이용하여 손상된 블록에 발생한 오류를 복구하는 오류 은닉 방법을 제안하였다. 그러나 정합벡터를 구하기 위해서 손상된 블록을 중심으로 상위와 하위에 있는 블록의 경계면에 있는 모든 픽셀에 대한 MAD (Mean Absolute Difference)값을 계산해야 하기 때문에 이 방법은 많은 연산량이 필요하다. 많은 연산량을 해결하기 위해서 본 논문에서는 계층 탐색 기반의 고속 오류 은닉 방법을 제안한다. 제안하는 방법에서는 정합벡터를 찾기 위한 확인점을 줄여 계산량을 감소시킨다. 제안한 방법을 Hsia가 제안한 방법과 비교하였을 때 화질을 유지하면서 연산량을 약 3배 줄일 수 있었다.

• Journal of Photoscience
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• 제12권1호
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• pp.1-9
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• 2005

The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal $Zn^2+$-binding RING-finger domain, a coiled-coil structure, and WD40 repeats at the C-terminal, implying that the protein plays a role in protein-protein interactions. The PnCOP1 transcript was detected in the cotyledon, hypocotyls and leaves, but not in root. The levels of the PnCOP1 transcript were reduced in leaves that were a farther distance away from the cotyledons. The expression level of the PnCOP1 gene was inhibited by light, while the expression was increased in the dark. During the floral inductive 16 hour-dark period for Pharbitis nil, the expression was increased and it reached its maximum at the 12th hour of the dark period. The levels of PnCOP1 mRNA were dramatically reduced upon light illumination. These results suggest that PnCOP1 may play an important function in the floral induction of Pharbitis nil.

• IMMUNE NETWORK
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• 제24권2호
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• pp.3.1-3.23
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• 2024

Cigarette smoke extract (CSE)-treated mouse airway epithelial cells (MAECs)-derived exosomes accelerate the progression of chronic obstructive pulmonary disease (COPD) by upregulating triggering receptor expressed on myeloid cells 1 (TREM-1); however, the specific mechanism remains unclear. We aimed to explore the potential mechanisms of CSE-treated MAECs-derived exosomes on M1 macrophage polarization and pyroptosis in COPD. In vitro, exosomes were extracted from CSE-treated MAECs, followed by co-culture with macrophages. In vivo, mice exposed to cigarette smoke (CS) to induce COPD, followed by injection or/and intranasal instillation with oe-TREM-1 lentivirus. Lung function and pathological changes were evaluated. CD68⁺ cell number and the levels of iNOS, TNF-α, IL-1β (M1 macrophage marker), and pyroptosis-related proteins (NOD-like receptor family pyrin domain containing 3, apoptosis-associated speck-like protein containing a caspase-1 recruitment domain, caspase-1, cleaved-caspase-1, gasdermin D [GSDMD], and GSDMD-N) were examined. The expression of maternally expressed gene 3 (MEG3), spleen focus forming virus proviral integration oncogene (SPI1), methyltransferase 3 (METTL3), and TREM-1 was detected and the binding relationships among them were verified. MEG3 increased N6-methyladenosine methylation of TREM-1 by recruiting SPI1 to activate METTL3. Overexpression of TREM-1 or METTL3 negated the alleviative effects of MEG3 inhibition on M1 polarization and pyroptosis. In mice exposed to CS, EXO^-CSE further aggravated lung injury, M1 polarization, and pyroptosis, which were reversed by MEG3 inhibition. TREM-1 overexpression negated the palliative effects of MEG3 inhibition on COPD mouse lung injury. Collectively, CSE-treated MAECs-derived exosomal long non-coding RNA MEG3 may expedite M1 macrophage polarization and pyroptosis in COPD via the SPI1/METTL3/TREM-1 axis.

• 방송공학회논문지
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• 제11권3호
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• pp.336-348
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• 2006

최근 멀티미디어 부호화 기술 및 전송기술의 급격한 발전과 DMB(Digital Multimedia Broadcasting)와 같은 이동형 TV의 등장으로 인해 작은 LCD 패널을 통해 시청하는 사용자가 증가하고 있다. 본 논문에서는 위와 같은 이동형 단말에서의 축구 동영상 시청 시 쾌적한 시청 경험을 제공하기 위한 지능형 디스플레이 기술을 소개하고 이러한 기술의 필요성을 조사, 분석하였다. 111명의 실험자를 대상으로 실시된 이 연구에서는 쾌적한 축구 동영상 시청을 보장하기 위한 최소화면 크기를 조사하였으며, 또한 화면 내에서 관심 영역만을 추출하여 디스플레이 하는 경우의 시청자 만족도의 변화를 알아보았다. 실험 결과는 영상의 해상도가 $320{\times}240$ 이하로 감소되는 경우, 관심 영역만을 확대하여 시청하는 것이 시청자의 시청 만족도를 향상시켜 주는 것으로 나타났으며, 따라서 필요시 관심 영역만을 디스플레이 해줄 수 있는 지능형 디스플레이 기술이 필요함을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1344-1349
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• 2004

Titin-cap (TCAP), one of the abundant transcripts in skeletal muscles, was nvestigated in this study in cattle because of its role in regulating the proliferation and differentiation of myoblasts by interacting with the myostatin gene. From the 5, and 3, RACE experiments, full-length TCAP coding sequence was identified, comprising 166 amino acids. The amino acid comparison showed high sequence similarities with previously identified human (95.8%) and mouse (95.2%) TCAP genes. The TCAP expression, addressed by northern blot, is limited in muscle tissues as indicated by Valle et al. (1997). The radiation hybrid analysis localized the gene on BTA19, where the comparative human and porcine counterparts are on HSA17 and SSC12. A few muscle-related genetic disorders were mapped on HSA17 and some growth-related QTLs were identified on SSC12. The bovine TCAP gene found in this study opens up new possibilities for the investigation of muscle-related genetic diseases as well as meat yield traits in cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.315-320
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• 2016

Porcine beta-defensin-1 (PBD-1) gene plays an important role in the innate immunity of pigs. The peptide encoded by this gene is an antimicrobial peptide that has direct activity against a wide range of microbes. This peptide is involved in the co-creation of an antimicrobial barrier in the oral cavity of pigs. The objective of the present study was to detect polymorphisms, if any, in exon-1 and exon-2 regions of PBD-1 gene in Large White Yorkshire (LWY) and native Ankamali pigs of Kerala, India. Blood samples were collected from 100 pigs and genomic DNA was isolated using phenol chloroform method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. Exon-1 and exon-2 regions of PBD-1 gene were amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Subsequent silver staining of the polyacrylamide gels revealed three unique SSCP banding patterns in each of the two exons. The presence of single nucleotide polymorphisms (SNPs) was confirmed by nucleotide sequencing of the PCR products. A novel SNP was found in the 5'-UTR region of exon-1 and a SNP was detected in the mature peptide coding region of exon-2. In exon-1, the pooled population frequencies of GG, GT, and TT genotypes were 0.67, 0.30, and 0.03, respectively. GG genotype was predominant in both the breeds whereas TT genotype was not detected in LWY breed. Similarly, in exon-2, the pooled population frequencies of AA, AG, and GG genotypes were 0.50, 0.27, and 0.23, respectively. AA genotype was predominant in LWY pigs whereas GG genotype was predominant in native pigs. These results suggest that there exists a considerable genetic variation at PBD-1 locus and further association studies may help in development of a PCR based genotyping test to select pigs with better immunity.

• 대한전자공학회논문지TC
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• 제37권1호
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• pp.76-87
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• 2000

멀티캐리어 DS-CDMA는 다중경로 페이딩 채널에서 광대역 시스템의 구현에 효과적인 방법이다. 본 논문에서는 콘볼류션 코딩을 적용한 중복된 멀티캐리어 DS-CDMA 시스템을 제안하고, 기존의 멀티캐리어 시스템과 비교하여 성능의 우수성을 입증한다. 제안된 멀티캐리어 DS-CDMA 시스템을 분할된 각 서브밴드를 가용주파수 대역 내에서 보다 효율적으로 구성하기 위하여 50% 중복 (overlapping) 된 구조를 갖도록 한다. 그리고 전송률이 1/M인 콘볼루션 채널코딩과 반복율이 1/R인 반복코딩을 적용하여 채널 코딩에 의한 이득과 주파수 다이버시티 (frequency diversity) 효과를 동시에 획득하고, 또한 MUI(Multiple User Interference)를 효과적으로 감소시키기 위하여 각 서브밴드의 확산신호에 raised-cosine 칩 성형필터를 적용하였다. 사용된 칩 성형필터의 rolloff 인자 (0< ${\beta}{\le}1$)의 변화에 따른 MUI를 분석하여 기존의 멀티캐리어 시스템의 성능보다 우수함을 보인다.