• Journal of Microbiology and Biotechnology
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• v.5 no.1
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• pp.6-13
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• 1995

A gene coding for the $\beta$-galactosidase (lactase) of Kluyveromyces tragilis UCD 55-55 was isolated by complementation in Escherichia coli YMC9. From the plasmid library made from Sau3A-digested chromosomal DNA, one positive clone was selected. The cloned gene for $\beta$-galactosidase was on 7.3 kilobase pair DNA fragment, and a slightly low level of $\beta$-galactosidase enzyme activity was detecied in E. coli. It was also confirmed that the cloned gene comes from K. tragilis by DNA-DNA hybridization and immunochemical blotting experiments. In order to construct a new yeast strain having the metabolic ability for lactose, the cloned gene for K. tragilis $\beta$-galactosidase was inserted in yeast vector YEp24 and YRp17, and transformed into Saccharomyces cerevisiae YNN27 and Ml-2B. The yeast transformants showed the nearly the same $\beta$-galactosidase productivity as level of K. tragilis when uninduced, but these could not utilize lactose as a sole carbon source, presumably due to the lack of lactose transport system. Nevertheless, a slightly higher ethanol productivity was achieved by these transformants than S. cerevisiae or K. tragilis, in the medium containing glucose and lactose.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2022.06a
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• pp.1213-1215
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• 2022

몰입형 비디오 부호화를 위한 MIV(MPEG Immersive Video) 표준은 제한된 3D 공간의 다양한 위치의 뷰(view)들을 효율적으로 압축하여 사용자에게 임의의 위치 및 방향에 대한 6 자유도(6DoF)의 몰입감을 제공한다. MIV 의 참조 소프트웨어인 TMIV(Test Model for Immersive Video)에서는 복수의 뷰 간 중복되는 영역을 제거하여 전송할 화소수를 줄이기 때문에 복호화기에서 렌더링(rendering)을 위해서 각 화소의 점유(occupancy) 정보도 전송되어야 한다. TMIV 는 점유맵을 깊이(depth) 아틀라스(atlas)에 포함하여 압축 전송하고, 부호화 오류로 인한 점유 정보 손실을 방지하기 위해 깊이값 표현을 위한 동적 범위의 일부를 보호대역(guard band)으로 할당한다. 이 보호대역을 줄여서 더 넓은 깊이값의 동적 범위를 사용하면 렌더링 화질을 개선시킬 수 있다. 따라서, 본 논문에서는 현재 TMIV 의 점유 정보 오류 분석을 바탕으로 이를 보정하는 기법을 제시하고, 깊이 동적 범위 확장에 따른 부호화 성능을 분석한다. 제안기법은 기존의 TMIV 와 비교하여 평균 1.3%의 BD-rate 성능 향상을 보여준다.

• Research in Mathematical Education
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• v.26 no.3
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• pp.253-270
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• 2023

This study aims to investigate the extent to which Korean high school textbooks incorporate opportunities for students to engage in the mathematical modeling process through tasks related to exponential and logarithmic functions. The tasks in three textbooks were analyzed based on the actions required for each stage in the mathematical modeling process, which includes identifying essential variables, formulating models, performing operations, interpreting results, and validating the outcomes. The study identified 324 units across the three textbooks, and the reliability coefficient was 0.869, indicating a high level of agreement in the coding process. The analysis revealed that the distribution of tasks requiring engagement in each of the five stages was similar in all three textbooks, reflecting the 2015 revised curriculum and national curriculum system. Among the 324 analyzed tasks, the highest proportion of the units required performing operations found in the mathematical modeling process. The findings suggest a need to include high-quality tasks that allow students to experience the entire process of mathematical modeling and to acknowledge the limitations of textbooks in providing appropriate opportunities for mathematical modeling with a heavy emphasis on performing operations. These results provide implications for the development of mathematical modeling activities and the reconstruction of textbook tasks in school mathematics, emphasizing the need to enhance opportunities for students to engage in mathematical modeling tasks and for teachers to provide support for students in the tasks.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.145-153
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• 2022

Mercury (Hg) is a major concern in marine environment because of their bioaccumulation and biomagnification properties, and adverse effects to aquatic organisms at even a trace amount. However, little information on the effects of Hg, compared to other heavy metals, is available in marine small crustaceans. Here, we investigated the transcriptional modulation of metabolism-related genes in the brackish water flea, Diaphanosoma celebensis after exposure to sublethal concentration (0.2, 0.4, 0.8 ㎍/l) of HgCl₂ for 48 h. Relative mRNA expression levels of five detoxification enzyme-coding genes (cytochrome P450; cyp360A1, cyp361A1, cyp4AP3, cyp4C122, and cyp370C5) and six digestive enzyme-coding genes [alpha amylase (AMY), alpha amylase related protein (AMY-like), trypsin (TRYP), chymotrypsin-like protein (CHY), lipase (LIP), pancreatic lipase-related protein (PLRP)] were analyzed using quantitative real time reverse transcription polymerase chain reaction (qRT-PCR). As results, Hg increased the mRNA level of cyp370C5 (clan2) and cyp4AP3 (clan4) in a concentration dependent manner. A significant increase in TRYP mRNA was also concentration-dependently observed after exposure to Hg. These findings suggest that cyp370C5 and cyp4AP3 play a key role in Hg detoxification in D. celebensis, and Hg can affect energy metabolism by modulating the transcription of digestive enzyme. This study will provide better understanding the molecular effects of Hg in marine small crustacean.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.214-214
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• 2022

Soluble starch synthases (SSs) elongate α-glucans from ADP-Glc to the glucan nonreducing ends and play a critical role in synthesizing resistant starch in the rice. A total of 10 SSs isoforms were reported in rice, including granules-bound starch synthase I (GBSSI), GBSSII, starch synthase I (SSI), SSIIa (SSII-3), SSIIb (SSII-2), SSIIc (SSII-1), SSIIIa (SSIII-2), SSIIIb (SSIII-1), SSIVa (SSIV-1), and SSIVb (SSIV-2). SSIII proteins are involved in forming the B chain and elongating cluster filling chains in amylopectin metabolism. The functions of SSIIIb (SSIII-1) are less clear as compared to SSs. Here, we sought to shed light on the genetic diversity profiling of the SSIII-1 gene in 374 rice accessions composed of 54 wild-type accessions and 320 bred cultivars (temperate japonica, indica, tropical japonica, aus, aromatic, and admixture). In total, 17 haplotypes were identified in the SSIII-1 coding region of 320 bred cultivars, while 44 haplotypes were detected from 54 wild-type accessions. The genetic diversity indices revealed the most negative Tajima's D value in the temperate-japonica, followed by the wild type, while Tajima's D values in other ecotypes were positive, indicating balancing selection. Nucleotide diversity in the SSIII-1 region was highest in the wild group (0.0047) while lowest in temperate-japonica. Lower nucleotide diversity in the temperate-japonica is evidenced by the negative Tajima's D and suggested purifying selection. The fixation index (F_ST) revealed a very high level of gene flow (low F_ST) between the tropical-japonica and admixture groups (F_ST=-0.21) followed by admixture and wild groups (-0.04), indica and admixture groups (0.02), while low gene flow with higher F_ST estimates between the temperate-japonica and aus groups (0.72), tropical-japonica and aromatic groups (0.71), and temperate-japonica and admixture groups (0.52). Taken together, our study offers insights into haplotype diversity and evolutionary fingerprints of SSIII-1. It provides genomic information to increase the resistant starch content of cooked rice.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.6A
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• pp.618-627
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• 2002

In processing images using 2 dimensional Discrete Wavelet Transform(2D-DWT), the method to process the pixels around the image boundary may affect the quality of image and the cost to implement in hardware and software. This paper proposed an effective method to treat the boundary pixels, which is apt to implement in hardware and software without losing the quality of the image costly. This method processes the 2-D image as 1-D array so that 2-D DWT is performed by considering the image with the serial-sequential data structure (Serial-Sequential Processing). To show the performance and easiness in implementation of the proposed method, an image compression codec which compresses image and reconstructs it has been implemented and experimented. It included log-scale fried quantizer, but the entropy coder was not implemented. From the experimental results, the proposed method showed the SNR of almost the same SNR(Signal to Noise Ratio) to the Periodic Expansion(PE) method when the compression ratio(excluding entropy coding) of 2:1, 15.3% higher than Symmetric Expansion(SE) method, and 9.3% higher than 0-pixel Padding Expansion(ZPE) method. Also PE method needed 12.99% more memory space than the proposed method. By considering only the compression process, SE and ZPE methods needed additional operations than the proposed one. In hardware implementation, the proposed method in this paper had 5.92% of overall circuit as the control circuit, while SE, PE, and ZPE method has 22%, 21,2%, and 11.9% as the control circuit, respectively. Consequently, the proposed method can be thought more effective in implementing software and hardware without losing any image quality in the usual image processing applications.

• ALGAE
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• v.19 no.3
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• pp.161-174
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• 2004

Phonological, morphological and molecular characteristics of Spatoglossum pacificum Yendo are examined. S. pacificum has an annual life cycle composed of saprophytes with apparent absence of male and female gametophytes in Korea. The seasonal growth of this species explains that the annual growth is closely related to the monthly variation of water temperature. S. pacificum has protruding reproductive structures above the outmost cortical layer. Although this observation is restricted to several species, reproductive structures on the thallus can make S. pacificurn distinguishable from S. crassum and S. lacturn. The morphogenesis of a midrib at the base of S. pacificum in this study is the same as those of Dictyopteris but different from those of S. crassum and S. lacturn, suggesting that S. pacificum is closely related to Dictyopteris. In the comparison of plastid gene sequences among species of Spatoglossum and Dictyopteris, S. pacificum is more similar to D. divaricata and D. undulate than those of S. crassum in rbcL, rbcS, psbA and psaA. This result is congruent with the anatomical characteristic of a midrib at the base of the thallus and the protrusion of reproductive organs on the thallus. The phylogenetic relationship based on these plastid genes also shows that S. pacifism is included in Dictyotpteris Glade and separated from S. crassum. We propose the new combination of Dictyopteris pacifica (Yendo) I.K. Hwang, H.S. Kim et W.J. Lee, comb. nov. based on the differences of anatomical characteristics of the midrib, the existence of reproductive organs on thallus and the molecular analyses.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.4 no.3
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• pp.517-526
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• 2000

In this paper, we propose the trellis coded spread spectrum communication system with one channel signal selection of the subset by the PN code. This paper proposes the Viterbi decoder that have the squared Euclidean distance of the order phase difference as well as 1st order phase difference as the branch metrics by using the multiple symbol detection method. TCM method was developed to overcome limited power and bandwidth efficiently in digital communication. we multiply one of convolution code's output data to PN code for applying TCM to the spread spectrum. We investigated the performance of the direct sequence/spread spectrum communication system with trellis coded modulation. In this system, we could improved the coding gain in the spread spectrum.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.5A
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• pp.503-509
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• 2007

Low-power motion estimation is required for video coding in portable information devices. In this paper, we propose a low-power motion estimation algorithm and 1-D systolic may VLSI architecture using full search block matching algorithm (FSBMA). Main power dissipation sources of FSBMA are complex computations and frequent memory accesses for data in the search area. In the proposed algorithm, memory accesses and computations are reduced by using 1D PE (processing array) array architecture performing motion estimation of two neighboring blocks in parallel and by skipping unnecessary computations during motion estimation. The VLSI implementation results of the algorithm show that the proposed VLSI architecture can save 9.3% power dissipation and can operate two times faster than an existing low-power motion estimator.

• Journal of the Korean Home Economics Association
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• v.35 no.5
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• pp.73-102
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• 1997

The purpose of this study was to identify the specific and empirical contents in terms of first-married stepmother about her stress and efforts to adapt to her stepfamily. This studies relied on the qualitative research method in order to approach stepmother's subjective experience. There were 6 stepmother respondents who were 6 first-marrieds living in Seoul or Kyoungki area. The data was collected by in-depth personal interview.‘Open coding process’, a part of grounded theory procedures were used to analyzed the data. The major findings can be summarized as follows: 1. When focused o stressors, the stress of stepmothers were categorized into 4 categories. Those were the following: 1 When focused on stressors, the stress of stepmothers were categorized into 4 categories. Those were the following: (1) Stepmother's own problem such as stepmother' identity problem; (2) Problems within stepfamily such as problems about relations with husbands, stepchildren, and her own children; (3) Problems of stepmother family's kin such as relational problems with former spouses, in-laws, and her parent; (4) Problems of social relationship such as isolation and alienation in social relationship and neighbors' prejudice about stepmother. 2. In overcoming stress from stepfamily life and adapting to it, 4 categories were identified as important. Contents of 4 categories were such like this: (1) Stepmother's own efforts - (a) give a positive meaning to marriage or remarriage (b) flexible personality (c) situation-accepting attitude (d) exclusive management of household income (e) job (f) perseverance for time to adaptation; (2) Positive feedback within stepfamily - (a) supportive husband (b) sense of unity as a couple (c) psychological reward from stepchildren (d) existence of stepmother's own child (e) behavior for family solidarity (f) family-centered religious life; (3) Stepfamily's relationship with former spouses such as breakdown in relationship with ex-wives or ex-husbands; (4) Support from stepmothers' kin and people in social network with stepmothers. It was concluded that to reduce stress and to help adapt to stepfamily, stepmother should make an effort, and that her stepfamily and her kin should cooperate and support. In addition, negative stereotype of stepmother and stepfamily should disappeared. It was suggested that studies on stepfamily should be vitalized and that programs for preparing remarriage and counseling for stepfamily should be spread.