• Title/Summary/Keyword: 3D PD

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Optimization and Scale-up of Fish Skin Peptide Loaded Liposome Preparation and Its Storage Stability (어피 펩타이드 리포좀 대량생산 최적 조건 및 저장 안정성)

  • Lee, JungGyu;Lee, YunJung;Bai, JingJing;Kim, Soojin;Cho, Youngjae;Choi, Mi-Jung
    • Food Engineering Progress
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    • v.21 no.4
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    • pp.360-366
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    • 2017
  • Fish skin peptide-loaded liposomes were prepared in 100 mL and 1 L solution as lab scales, and 10 L solution as a prototype scale. The particle size and zeta potential were measured to determine the optimal conditions for the production of fish skin peptide-loaded liposome. The liposome was manufactured by the following conditions: (1) primary homogenization at 4,000 rpm, 8,000 rpm, and 12,000 rpm for 3 minutes; (2) secondary homogenization at 40 watt (W), 60 W, and 80 W for 3 minutes. From this experimental design, the optimal conditions of homogenization were selected as 4,000 rpm and 60 W. For the next step, fish peptides were prepared as the concentrations of 3, 6, and 12% at the optimum manufacturing conditions of liposome and stored at $4^{\circ}C$. Particle size, polydispersion index (pdI), and zeta potential of peptide-loaded liposome were measured for its stability. Particle size increased significantly as manufacture scale and peptide concentration increased, and decreased over storage time. The zeta potential results increased as storage time increased at 10 L scale. In addition, 12% peptide showed the formation of a sediment layer after 3 weeks, and 6% peptide was considered to be the most suitable for industrial application.

Molecular Characterization of a Nuclease Gene of Chlorella Virus SS-2

  • Park, Yun-Jung;Jung, Sang-Eun;Choi, Tae-Jin
    • The Plant Pathology Journal
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    • v.25 no.1
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    • pp.47-53
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    • 2009
  • Sequence analysis of the Chlorella virus SS-2 revealed one putative nuclease gene that is 807 bp long and encodes a 31kDa protein. Multiple sequence alignment analysis reveals the presence of highly conserved PD-(D/E)XK residues in the encoded protein. The gene cloned into an expression vector was expressed as a His-tagged fusion protein in chaperone containing pKJE7 cells. The recombinant protein was purified using a His-Trap chelating HP column and used for functional analysis. Exonuclease activity of the SS-2 nuclease was detected when the DNA substrates, such as linear ssDNA, PCR amplicon, linear dsDNA with 5'-overhang ends, 3'-overhang ends, or blunt ends were used. Covalently closed circular DNA was also degraded by the SS-2 recombinant protein, suggesting that the SS-2 nuclease has an endonuclease activity. Stable activity of SS-2 nuclease was observed between $10^{\circ}C$ and $50^{\circ}C$. The optimum pH concentrations for the SS-2 nuclease were pH 6.0-8.5. Divalent ions inhibited the SS-2 nuclease activity.

Development of UV-IR Camera using IR Module and Improved UV Filter Transmittance (투과율 향상 UV 필터와 IR 모듈을 이용한 자외선-적외선(UV-IR) 카메라 개발)

  • Kim, Young-Seok;Shong, Kil-Mok;Bang, Sun-Bae;Kim, Chong-Min;Choi, Myeong-Il;Kim, Woo-Jin
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.26 no.12
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    • pp.37-43
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    • 2012
  • UV-IR diagnostic technology is being used for predictive maintenance of high voltage equipment together with IR-thermography and ultrasonic devices. In this paper, the UV-IR camera design, fabrication, and perform a simple test to be take advantage of the diagnostic equipment. UV-IR camera developed a $6.4^{\circ}{\times}4.8^{\circ}$ of the field of view as a conventional camera to diagnose a wide range of slightly enlarged, and power equipment to measure the distance between the camera and the distance meter has been attached. In a distance of 5m with the UV-IR it is possible to detect partial discharge with a PD level of 2.5 pC and a RIV(Radio Influence Voltage) level of $3.6dB{\mu}V$.

Identification of Rhizo-bacterium Inhibiting Diaporthe citri Causing Citrus Melanose (감귤 검은점무늬병균의 생육을 저해하는 근권 세균의 분리 및 동정)

  • Nnam, Myung-Hyeun;Shin, Jin-Ho;Choi, Jae-Pill;Hong, Suck_Il;Kim, Young-Gwon;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.332-335
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    • 2009
  • Rhizo-bacteria were isolated from organic-farming soils to select antagonistic agent for controlling citrus melanose disease. Among several antagonistic bacteria, KB-401 effectively inhibited mycelial growth of several plant fungal pathogens, including the pathogen of citrus melanose, Diaporthe citri. KB-401 also inhibited spore germination of the fungal pathogen. The tip of germ tube was swollen when conidia of D. citri were co-culture with KB-401 in PD broth amended 1% glucose. KB-401 was identified as Bacillus subtilis through the investigation for physiological characters and the analysis of nucleotide sequences of 16S rDNA.

Phase II Study of Docetaxel (Aisu) Combined with Three-dimensional Conformal External Beam Radiotherapy in Treating Patients with Inoperable Esophageal Cancer

  • Shen, Kang;Huang, Xin-En;Lu, Yan-Yan;Wu, Xue-Yan;Liu, Jin;Xiang, Jin
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.12
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    • pp.6523-6526
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    • 2012
  • Objective: This study was designed to investigate treatment efficacy and side effects of concomitant Aisu$^{(R)}$ (docetaxel) with three-dimensional conformal external beam radiotherapy for the treatment of inoperable patients with esophageal cancer. Methods: Inoperable patients were treated with three-dimensional conformal external beam radiotherapy (5/week, 2 GY/day, and total dose 60GY) plus docetaxel ($30-45mg/m^2$, iv, d1, 8). Results: Twenty eight patients met the study eligibility criteria and the response rate was evaluated according to RICIST guidelines. Among 28 patients, 2 achieved CR, 22 PR, 3 SD and 1 patient was documented PD. Mild gastrointestinal reaction and bone marrow suppression were also documented. All treatment related side effects were tolerable. Conclusion: Three-dimensional conformal external beam radiotherapy combined with docetaxel is an active and safe regimen for inoperable patients with esophageal cancer.

Development of UV Corona Camera for the Detecting of Discharge on Power Facility using UV Transmittance Improvement Filter (UV 투과율 향상 필터 기술을 이용한 전력설비 방전 검출용 자외선 코로나 카메라 개발)

  • Kim, Young-Seok;Choi, Myeong-Il;Kim, Chong-Min;Bang, Sun-Bae;Shong, Kil-Mok
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.11
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    • pp.1656-1661
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    • 2012
  • UV inspection technology is being used for predictive maintenance of power facility together with IR-thermography and ultrasonic devices. In this paper, the UV corona camera design, fabrication, and perform a simple test to be take advantage of the diagnostic equipment. The UV corona camera developed a $6.4^{\circ}{\times}4.8^{\circ}$ of the field of view as a conventional camera to diagnose a wide range of slightly enlarged, and power equipment to measure the distance between the camera and the distance meter has been attached. The transmission between 250 to 280nm was 11% ($12.5%{\times}88%{\times}98%$) by combing the transmission on absorption film, window and other filter(UG 5, nickel sulphate and so on). In a distance of 5m with the UV corona camera it is possible to detect partial discharge with a PD level of 2.5pC and a RIV level of $3.6dB{\mu}V$.

Thermal conductivity and properties of sheath alloy for High-$T_c$ superconductor tape (고온초전도 선재용 피복합금의 열전도도 측정 및 특성평가)

  • 박형상;지봉기;김중석;임준형;오승진;오승진;주진호;나완수;유재무
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.13 no.8
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    • pp.711-717
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    • 2000
  • Effect of alloying element additions to Ag on thermal conductivity electrical conductivity and mechanical properties of sheath materials for BSCCO tapes has been characterized. The thermal conductivity at low temperature range(10~300K) of Ag alloys were evaluated by both direct and indirect measurement techniques and compared with each other. It was observed that thermal conductivity decreased with increasing the content of alloying elements such as Au, Pd and Mg. Thermal conductivity of pure Ag at 30 K was measured to be 994.0 W/m.K on the other hand the corresponding values of A $g_{0.9995}$/M $g_{0.0005}$, A $g_{0.974}$/A $u_{0.025}$/M $g_{0.001}$, A $g_{0.973}$/Au.0.025//M $g_{0.002}$, and A $g_{0.92}$/P $d_{0.06}$/M $g_{0.02}$ were 342.6, 62.1, 59.2, 28.9 W/m.K respectively indicating 3 to 30 times lower than that of pure Ag. In addition alloying element additions to Ag improved mechanical strength while reduced elongation probably due to the strengthening mechanisms by the presence of additive atoms.s.

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THz Short-range Transmission Technology Based on Photonics (포토닉스 기반 THz 근거리 전송 기술)

  • Cho, Seung-Hyun;Moon, Sang-RoK;Lee, Joon Ki
    • Electronics and Telecommunications Trends
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    • v.34 no.6
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    • pp.61-70
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    • 2019
  • Recently, research and development for next-generation mobile communication and short-range wireless communication has begun worldwide along with the provision of commercial services of 5G mobile communication technology. In response to this trend, the THz band has attracted considerable attention as a frequency band for transmitting 100 Gbps of large-capacity wireless data. For communication in the THz band, research and development of approaches based on photonics and electronics is being actively performed; the configurations, characteristics, and performances of these two methods for THz transmission have been seriously examined. Among them, we reviewed the technical issues in implementing THz wireless transmission technology using photonics technology. We also introduced the Electronic and Telecommunications Research Institute's (ETRI) development of photonics-based THz short-range transmission technology starting from 2019 and including some initial results. In the near future, 100 Gbps high-capacity wireless data transmission technology utilizing photonics technology is expected to be commercially available and applied to various applications, such as 3D hologram transmission, uncompressed large capacity medical data transmission, and multiple augmented reality/virtual reality (AR/VR).

A Study on the Improvement Method of Indoor Light Environment Items in Environmental Certification for Educational Facilities - Focusing on G-SEED, LEED, BREEAM, CASBEE - (교육시설 친환경 인증에서 실내 빛환경 항목 개선방안에 관한 연구 - G-SEED, LEED, BREEAM, CASBEE를 중심으로 -)

  • Lee, Se-Won;Lee, Kyung Sun;Kim, Sooran
    • Journal of the Architectural Institute of Korea Planning & Design
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    • v.34 no.5
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    • pp.61-69
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    • 2018
  • The goal of this study is to compare the evaluation results of indoor daylight environment for each certification and propose improvement points. As the importance of indoor daylight environment has increased, foreign countries have been aware of this and have changed the light environmental certification items. On the other hand, Korea environmental certification systems have not been able to follow the current situation. In order to improve this, this study intended to find evaluation criteria for each category by comparing and analyzing indoor daylight environment certification items in Korea's G-SEED, LEED in the United States, BREEAM in the UK, and CASBEE in Japan. After that, this study simulated 3D models of three spaces about classroom, reading room and club room of Yanghyun elementary School which is scheduled to be constructed for the evaluation of indoor daylight environment, and the results were analyzed to suggest for improvement.

Dominant-species Variation of Soil Microbes by Temperate Change (온도변화에 기인한 토양미생물 우점종의 변화에 관한 연구)

  • Park, Kap-Joo;Lee, Byeong-Chol;Lee, Jae-Seok;Park, Chan-Sun;Cho, Myung-Hwan
    • Korean Journal of Environmental Biology
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    • v.29 no.1
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    • pp.52-60
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    • 2011
  • Today, the weather is changing continually, due to the progress of global warming. As the weather changes, the habitats of different organisms will change as well. It cannot be predicted whether or not the weather will change with each passing day. In particular, the biological distribution of the areas climate change affects constitutes a major factor in determining the natural state of indigenous plants; additionally, plants are constantly exposed to rhizospheric microorganisms, which are bound to be sensitive to these changes. Interest has grown in the relationship between plants and rhizopheric microorganisms. As a result of this interest we elected to research and experiment further. We researched the dominant changes that occur between plants and rhizospheric organisms due to global warming. First, we used temperature as a variable. We employed four different temperatures and four different sites: room temperature ($27^{\circ}C$), $+2^{\circ}C$, $+4^{\circ}C$, and $+6^{\circ}C$. The four different sites we used were populated by the following species: Pinus deniflora, Pinus koraiensis, Quercus acutissima, and Alnus japonica. We counted colonies of these plants and divided them. Then, using 16S rRNA analysis we identified the microorganisms. In conclusion, we identified the following genera, which were as follows: 10 species of Bacillus, 2 Enterobacter species, 4 Pseudomonas species, 1 Arthrobacter species, 1 Chryseobacterium species, and 1 Rhodococcus species. Among these genera, the dominant species in Pinus deniflora was discovered in the same genus, but a different species dominated at $33^{\circ}C$. Additionally, that of Pinus koraiensis changed in both genus and species which changed into the Chryseobacrterium genus from the Bacilus genus at $33^{\circ}C$.