• Reproductive and Developmental Biology
• /
• 제29권1호
• /
• pp.37-41
• /
• 2005

본 연구는 한우 수정란의 체외생산에 있어서 효율과 품질의 향상을 위해서, 미성숙 난포란을 회수하는 난소의 형태와 배양 용기로서 straw의 효과를 검토하였다. 난소의 형태에 따른 수정율은 전 군에서 70.3∼84.1%로서 비슷한 경향이었다. 8세포기 및 배반포기 발달율은 황체와 난포가 모두 존재하지 않는 대조군이 가장 높았다. 배반포의 inner cell mass(ICM), trophectoderm(TE), total cell number(TCN) 및 ICM/TCN 비율은 낭종군과 퇴행황체군이 다른군에 비하여 높은 경향이었다. 체외성숙에 이용하는 배양용기에 따른 수정율은 0.5 ㎖ straw 군이, 8세포기 발달율은 대조군이 가장 높았으나, 배반포기 발달율은 23.1∼30.7％로서 각 군 간에 비슷한 경향이었다. 한편 각각의 배양 용기에서 유래된 배반포의 ICM, TE, TCN 및 ICM/TCN 비율은 유사한 경향이었다.

• 폴리머
• /
• 제37권3호
• /
• pp.405-410
• /
• 2013

스티렌-에틸렌/부틸렌-스티렌(SEBS) 블록공중합체를 이용하여 선형의 폴리페닐렌설파이드(PPS)와 상용 폴리에틸렌테레프탈레이트 블렌드(PET)의 상용화를 하고자 하였다. 머독 혼련 스크류가 장착된 단축 압출기를 통하여 제조한 블렌드를 성형하여 그 특성들을 살펴보았다. 네 개의 각기 다른 PPS/PET 조성에 대하여 블렌드 수지를 제조한 후 열적, 유변학적, 기계적 특성을 측정하고 모폴로지를 관찰하였다. 평가한 결과 비혼화성계로서 심각한 물성 저하를 관찰할 수 있었다. 이 중 시장성과 경제성을 고려하여 PPS/PET(40/60)을 기초블렌드 조성으로 선정하고 상용화제로서 SEBS를 첨가하여 제조한 삼원블렌드의 물성을 측정한 결과 기초블렌드의 기계적 물성이 개선되었고 모폴로지 관찰결과 PPS 분산상 크기가 감소함을 보인 바 SEBS가 선형 PPS/PET 블렌드의 상용성을 향상시켰기 때문이라 사료된다.

• 대한치과기공학회지
• /
• 제29권2호
• /
• pp.49-64
• /
• 2007

This research collected the curriculum for Dental Technology from a total of 20 schools -3-year colleges and 4-year colleges- all in Korea. And we analyzed the average credits of subjects from students. As a result of this analysis, we get the conclusion below: 1. In a distribution which students answered about each subjects; Seminar, Dental morphology I II, Dental morphology practice I II, and Dental devices & instruments don't have many credits and mostly have a converged tendency in high points, according to the arithmetic mean of the major basis subjects. 2. In an analysis of the correlation which is based on the distinction of sex: Complete denture prosthodontics I II III, Dental orthodontics I II, Dental orthodontics practice I II, and Dental orthodontics & practice III have a meaningful difference. ($\rho$< 0.05 ) 3. In an analysis of the correlation which is based on an age The major basis subjects -Introduction to dental technology, Dental esthetic, and Dental casting procedure-, and the major application subjects -Crown and bridge prosthodontics practice, Complete denture prosthodontics, Dental orthodontics, Dental orthodontics practice, and Operative dentistry laboratory technology- have meaningful difference. ($\rho$< 0.05 ) 4. In a correlation coefficient between subjects; The correlation coefficient of the major application subjects is higher than the major basis subjects. According to the student - t, we found that the level of significance is $\rho$< 0.001, which is meaningful. We feel the lack of a limitation which we didn't survey all students who are major in Department of Dental Technology. And we also feel that we'd better to consider not only students who being in college but also graduated students.

• Imaging Science in Dentistry
• /
• 제42권3호
• /
• pp.155-161
• /
• 2012

Purpose: This study was performed to investigate the prevalence, morphology, and calcification pattern of the elongated styloid process in the Mathura population and its relation to gender, age, and mandibular movements. Materials and Methods: The study analyzed digital panoramic radiographs of 2,706 adults. The elongated styloid process was classified with the radiographic appearance based on the morphology and calcification pattern. The limits of mandibular protrusion were evaluated for each subject. The data were analyzed by using a Student's t-test and chi-squared test with significance set at p=0.05. Results: Bilateral elongation having an "elongated" type styloid process with a "partially mineralized" pattern was the most frequent type of styloid process. No correlation was found between styloid process type and calcification pattern on the one hand and gender on the other, although elongated styloid was more prevalent in older and male populations (p<0.05). Further styloid process elongation showed no effect on mandibular protrusive movement (p>0.05). Conclusion: Dentists should recognize the existence of morphological variation in elongated styloid process or Eagle syndrome apparent on panoramic radiographs. We found higher prevalence of elongated styloid process in the population of the Mathura region when compared with other Indian populations. The calcification of the styloid process was more common in the older age group with no correlation to gender, mandibular movement and site. "Type I" with a "partially calcified" styloid process was observed more frequently in the population studied.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
• /
• pp.108-108
• /
• 2003

The purpose of this is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine embryos. Blastocysts were produced by in vitro fertilization of slaughterhouse-derived, in vitro matured oocytes with frozen-thawed boar semen, and subsequent culture on granulosa cell monolayer. After frozen-thawing, embryos were culture in NCSU-23 medium with 5 mM hypotaurine, 4 mg/$m\ell$ BSA and 10 ng/$m\ell$ for 48 hrs to survival tests. When blastocysts were frozen-thawed by OPS methods, the embryos with normal morphology were 32.1, 34.5 and 38.9 % in early blastocyst, blastocyst and expanded blastocyat stages. The rates of partial damaged embryos were significantly (P<0.05) higher in early biastocysts than expanded blastocysts. In another experiment, the embryos frozen by OPS methods were cultured for 48 hrs for survival and developmental rates in vitro. The proportions of embryos hatched were 11.8, 20.2 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. On the other hand, The proportions of embryo with normal morphology after culture were 23.5, 25.0 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. These finding indicate the possible broader application for OPS methods that this procedure described is relatively harmless, that it can be used for blastocysts of different developmental stages.

• 한국재료학회지
• /
• 제21권11호
• /
• pp.611-616
• /
• 2011

Red-orange phosphors $Gd_{1-x}PO_4:{Eu_x}^{3+}$ (x = 0, 0.05, 0.10, 0.15, 0.20) were synthesized with changing the concentration of $Eu^{3+}$ ions using a solid-state reaction method. The crystal structures, surface morphology, and optical properties of the ceramic phosphors were investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), and photoluminescence (PL) spectrophotometry. The XRD results were in accordance with JCPDS (32-0386), and the crystal structures of all the red-orange phosphors were found to be a monoclinic system. The SEM results showed that the size of grains increases and then decreases as the concentration of $Eu^{3+}$ ionincreases. As for the PL properties, all of the ceramic phosphors, irrespective of $Eu^{3+}$ ion concentration, had orange and red emissions peaks at 594 nm and 613 nm, respectively. The maximum excitation and emission spectra were observed at 0.10 mol of $Eu^{3+}$ ion concentration, just like the grain size. An orange color stronger than the red means that $^5D_0{\rightarrow}^7F_1$ (magnetic dipole transition) is dominant over the $^5D_0{\rightarrow}^7F_2$ (electric dipole transition), and $Eu^{3+}$ is located at the center of the inversion symmetry. These properties contrasted with those of a red phosphor $Y_{1-x}PO_4:{Eu_x}^{3+}$, which has a tetragonal system. Therefore, we confirm that the crystal structure of the host material has a major effect on the resulting color.

• 한국재료학회지
• /
• 제29권9호
• /
• pp.559-566
• /
• 2019

$Lu(Nb,Ta)O_4:Eu^{3+}$ powders are synthesized by a solid-state reaction process using LiCl and $Li_2SO_4$ fluxes. The photoluminescence (PL) excitation spectra of the synthesized powders consist of broad bands at approximately 270 nm and sharp peaks in the near ultraviolet region, which are assigned to the $Nb^{5+}-O^{2-}$ charge transfer of $[NbO_4]^{3-}$ niobates and the f-f transition of $Eu^{3+}$, respectively. The PL emission spectra exhibit red peaks assigned to the $^5D_0{\rightarrow}^7F_J$ transitions of $Eu^{3+}$. The strongest peak is obtained at 614 nm ($^5D_0{\rightarrow}^7F_2$), indicating that the $Eu^{3+}$ ions are incorporated into the $Lu^{3+}$ asymmetric sites. The addition of fluxes causes the increase in emission intensity, and $Li_2SO_4$ flux is more effective for enhancement in emission intensity than is LiCl flux. The substitution of $Ta^{5+}$ for $Nb^{5+}$ results in an increase or decrease in the emission intensity of $LuNb_{1-x}Ta_xO_4:Eu^{3+}$ powders, depending on amount and kind of flux. The findings are explained using particle morphology, modification of the $[NbO_4]^{3-}$ structure, formation of substructure of $LuTaO_4$, and change in the crystal field surrounding the $Eu^{3+}$ ions.

• 대한치과교정학회지
• /
• 제53권2호
• /
• pp.77-88
• /
• 2023

Objective: To develop a method for generating three-dimensional (3D) digital models of the periodontal ligament (PDL) using 3D cone-beam computed tomography (CBCT) reconstruction and to evaluate the accuracy and agreement of the 3D PDL models in the measurement of periodontal bone loss. Methods: CBCT data collected from four patients with skeletal Class III malocclusion prior to periodontal surgery were reconstructed at three voxel sizes (0.2 mm, 0.25 mm, and 0.3 mm), and 3D tooth and alveolar bone models were generated to obtain digital PDL models for the maxillary and mandibular anterior teeth. Linear measurements of the alveolar bone crest obtained during periodontal surgery were compared with the digital measurements for assessment of the accuracy of the digital models. The agreement and reliability of the digital PDL models were analyzed using intra- and interexaminer correlation coefficients and Bland-Altman plots. Results: Digital models of the maxillary and mandibular anterior teeth, PDL, and alveolar bone of the four patients were successfully established. Relative to the intraoperative measurements, linear measurements obtained from the 3D digital models were accurate, and there were no significant differences among different voxel sizes at different sites. High diagnostic coincidence rates were found for the maxillary anterior teeth. The digital models showed high intra- and interexaminer agreement. Conclusions: Digital PDL models generated by 3D CBCT reconstruction can provide accurate and useful information regarding the alveolar crest morphology and facilitate reproducible measurements. This could assist clinicians in the evaluation of periodontal prognosis and establishment of an appropriate orthodontic treatment plan.

• 대한한의학회지
• /
• 제23권3호
• /
• pp.200-210
• /
• 2002

Objectives: Dojeckjiyu-tang has been used to treat Hwaseol & Jeokri. The object of this study is examination of the treatment effect of Dojeckjiyu-tang for ulcerative colitis of the mouse descending colon. Methods and Materials : Twenty-one rats were divided into 3 groups and treated as follows: the control group was untreated mice. The UCE group was ulcerative colitis elicited mice. The DJT group was Dojeckjiyu-tang treated mice after ulcerative colitis elicitation. The groups were examined with common morphology, paneth cells in intestinal crypt, absorptive cells and goblet cells in epithelium, cell division in mucose, COX-1 as mucosal protector, COX-2 (which appears to play an important role in inflammation), IL-2R-, ICMA-1-inducing cellular immuno-chainreaction, and the distribution of apoptotic cells. Results: 1. The morphology of colonic mucosa from UCE mice: the disappearance of epithelium and intestinal propria in hemorrhagic erosions were seen, but in the morphology of colonic mucosa from DJT-treated mice, the configuration of epithelium and intestinal propria were the same as normal. 2. The distribution of goblet cells and absorptive cells with microvilli in intestinal propria from UCE mice: a noticeable decrease of goblet cells and absorptive cells with microvilli were seen, but with the distribution of goblet cells and absorptive cells with microvilli in intestinal propria from DJT -treated mice, the configuration of goblet cells and absorptive cells with microvilli were the same as normal. 3. The immunohistochemical stain for BrdD in colonic mucosa and COX-1 in lamina propria from UCE mice: BrdU positive cells and COX-1 positive cells in the region of hemorrhagic erosion disappeared, but in the immunohistochemical stain for BrdU in colonic mucosa and COX-1 in lamina propria from DIT-treated mice, BrdU positive cells and COX-1 positive cells were seen. 4. The immunohistochemical stain for COX-2 in lamina propria, IL-2R-in lamina propria, intestinal propria and submucosa and ICMA-1 in intestinal propria and submucosa from DCE mice: a noticeable increase COX-2, IL-2R-, ICMA-1 positive cells were seen, but in the immunohistochemical stain for COX-2 in lamina propria, IL-2R-in lamina propria, intestinal propria and submucosa and ICMA-1 in intestinal propria and submucosa from DJT-treated mice, a numerical decrease of COX-2, IL-2R-, ICMA-1 positive cells was observed. 5. The distribution of apoptotic cells in epithelium and lamina propria from UCE mice: a noticeable increase of apoptotic cells in region of hemorrhagic erosion was seen, but in the distribution of apoptotic cells in epithelium and lamina propria from DJT-treated mice, a remarkable decrease of apoptotic cells was seen. Conclusions: According to the above results, Dojeckjiyu-tang has a moderate effect on ulcerative colitis in descending colon.

• 한국가금학회지
• /
• 제35권1호
• /
• pp.57-62
• /
• 2008

본 연구는 유기산 복합제(lactic acid 12%, formic acid 9%, citric acid 5%, butyric acid 5% and phosphoric acid 6%) 급여가 육계의 생산성, 장기무게, 장내 융모 형태 및 혈액내 면역적 특성에 미치는 영향을 알아보기 위해 시험을 실시하였다. 사양 시험은 2일령 Arbor Acre Broiler(male) 480수를 공시하였으며, 5주간 사양 시험을 실시하였다. 시험 설계는 1) CON(기초 사료), 2) OA1(기초 사료 + 유기산 복합제 0.1%) 및 3) OA2(기초 사료 + 유기산 복합제 0.2%)로 3개 처리를 하여 처리당 8반복, 반복당 20수씩 완전 임의 배치하였다. 전체 시험 기간동안 생산성은 처리구간 유의적인 차이가 없었다. 장기 무게에서는 근위 무게가 CON 처리구와 비교하여 OA2 처리구가 유의적으로 높게 나타내었다(Linear effect = 0.034). 하지만, 비장과 소장 무게에서는 처리구간 차이를 나타내지 않았다. 혈액내 면역적 성상에서는 처리구간 차이를 나타내지 않았다. 장의 융모에서는 CON 처리구와 비교하여 OA1 처리구가 유의적으로 높게 나타났다(Quadratic effect = 0.050). 결론적으로, 본 시험의 결과 육계에 0.2% 유기산 복합제를 급여시 근위의 중량을 증가시키고, 0.1% 급여 시는 소장 융모의 길이를 증가시키는 효과가 있었다.