• Journal of Animal Reproduction and Biotechnology
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• v.37 no.2
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• pp.136-143
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• 2022

Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1 µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.

• Archives of Plastic Surgery
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• v.49 no.3
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• pp.378-396
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• 2022

Several reconstructive methods have been reported to restore the continuity of the aerodigestive tract following resection of pharyngeal and hypopharyngeal cancers. However, high complication rates have been reported after voice prosthesis insertion. In this setting, the ileocolon free flap (ICFF) offers a tubularized flap for reconstruction of the hypopharynx while providing a natural phonation tube. Herein, we systematically reviewed the current evidence on the use of the ICFF for reconstruction of the aerodigestive tract. A systematic literature search was conducted across PubMed MEDLINE, Web of Science, ScienceDirect, Scopus, and Ovid MEDLINE(R). Data on the technical considerations and surgical and functional outcomes were extracted. Twenty-one studies were included. The mean age and follow-up were 54.65 years and 24.72 months, respectively. An isoperistaltic or antiperistaltic standard ICFF, patch flap, or chimeric seromuscular-ICFF can be used depending on the patients' needs. The seromuscular chimeric flap is useful to augment the closure of the distal anastomotic site. The maximum phonation time, frequency, and sound pressure level (dB) were higher with ileal segments of 7 to 15 cm. The incidence of postoperative leakage ranged from 0 to 13.3%, and the majority was occurring at the coloesophageal junction. The revision rate of the microanastomosis ranged from 0 to 16.6%. The ICFF provides a reliable and versatile alternative for reconstruction of middle-size defects of the aerodigestive tract. Its three-dimensional configuration and functional anatomy encourage early speech and deglutition without a prosthetic valve and minimal donor-site morbidity.

• Journal of the Korean Geotechnical Society
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• v.39 no.8
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• pp.17-28
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• 2023

Laboratory-scale hydraulic fracturing experiments were conducted on granite specimens at various viscosities and injection rates of the fracturing fluid. A series of cross-sectional computed tomography (CT) images of fractured specimens was obtained via a three-dimensional X-ray CT imaging method. Pixel-level fracture segmentation of the CT images was conducted using a convolutional neural network (CNN)-based Nested U-Net model structure. Compared with traditional image processing methods, the CNN-based model showed a better performance in the extraction of thin and complex fractures. These extracted fractures extracted were reconstructed in three dimensions and morphologically analyzed based on their fracture volume, aperture, tortuosity, and surface roughness. The fracture volume and aperture increased with the increase in viscosity of the fracturing fluid, while the tortuosity and roughness of the fracture surface decreased. The findings also confirmed the anisotropic tortuosity and roughness of the fracture surface. In this study, a CNN-based model was used to perform accurate fracture segmentation, and quantitative analysis of hydraulic stimulated fractures was conducted successfully.

• Journal of Forest and Environmental Science
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• v.39 no.1
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• pp.55-64
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• 2023

Properties of a lesser-used wood species were investigated to determine its potential for structural utilization. Trees of Delonix regia were felled and sampled at the base, middle and top and then sectioned to inner wood, middle wood, and outer wood for variation across the axial and radial directions. Hence, selected physical and mechanical properties as well as natural durability of D. regia along the radial and axial directions were examined. Obtained data were analyzed using analysis of variance (ANOVA) at α_0.05. There was no significant difference in the Moisture content (MC) of the wood but specific gravity (SG) decreased from base to top ranging from 0.35-0.44. Water absorption, volumetric swelling, and volumetric shrinkage range from 46.18-51.86%, 2.57-4.02%, and 2.26-3.96% respectively along the axial plane. The weight loss for graveyard exposure and accelerated laboratory decay test ranged from 25.14-48.00% and 32.02-44.45% respectively. Modulus of Rupture and Modulus of Elasticity values range from 29.42-72.68 Nmm² and 3,834.54-8,830.37 Nmm² respectively. The SG values has confirmed the species as a medium density wood and values of other properties tested showed that the wood is dimensional stable and moderately resistance to fungi and termite. Hence, it could be used for light construction purposes such as furniture and other interior woodwork.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.6A
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• pp.779-788
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• 2008

In this present study, the three dimensional shell elements analysis method for exact distortional behavior of multicell trapezoidal box girders subjected to an eccentric loading is proposed. In order to perform the independent distortional analysis using shell elements, it is necessary to calculate exact distortional forces. In this study, the force-decomposition equation for applied eccentric load acting on multicell trapezoidal box girder is derived and the equation based on static force equilibrium and superposition theory decompose the eccentric load to the loads cause flexture, torsion and distortion. So by using this force-decomposition equation and shell element analysis, each behavior can be easily analysis independently. This independent analysis method is very useful to physically understand each major behavior of multicell box girder, especially distortional phenomenon. Furthermore, it may be also very useful for designer to perform the independent distortional analysis for diaphragm design using simple 3D shell elements model without preliminary complex calculation for distortional constants.

• Restorative Dentistry and Endodontics
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• v.47 no.4
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• pp.39.1-39.12
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• 2022

Objectives: This study investigated the prevalence and characteristics of external cervical resorption (ECR) regarding sex, age, tooth, stages of progression, and portal of entry, using cone-beam computed tomography (CBCT) scans. Materials and Methods: CBCT scans of 1,313 patients from a Brazilian subpopulation comprising 883 female and 430 male patients (mean age, 55.2 years), acquired using a PreXion 3D CBCT unit, were evaluated. All permanent teeth included in the scans were evaluated for the presence of ECR according to the 3-dimensional classification and the portal of entry. The association between the presence of ECR and the factors studied was assessed using the χ² test. Intra-observer agreement was analyzed with the kappa test (α = 0.05). Results: In total, 6,240 teeth were analyzed, of which 84 (1.35%) were affected by ECR. A significant association was found between the presence of ECR and sex, with a higher prevalence in male patients (p = 0.002). The most frequently affected teeth were the mandibular and maxillary central incisors. The most common height was the mid-third of the root. For the portal of entry, 44% of cases were on the proximal surfaces, 40.5% on the lingual/palatal surface and 15.5% on the buccal surface. Intra-observer agreement was excellent. Conclusions: The prevalence of ECR was 1.35%, with a higher prevalence in male patients and a wide age distribution. The mandibular and maxillary central incisors were the most commonly affected teeth, and cases of ECR most frequently showed a height into the mid-third of the root and proximal entry.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.46 no.3
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• pp.285-292
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• 2023

As various accidents have occurred in underground spaces, we aim to improve the quality validation standards and methods as specified in the Regulations on Producing Integrated Map of Underground Spaces devised by the Ministry of Land, Infrastructure and Transport of the Republic of Korea for a high-quality integrated map of underground spaces. Specifically, we propose measures to improve the quality assurance of pipeline-type underground facilities, the so-called life lines given their importance for citizens' daily activities and their highest risk of accident among the 16 types of underground facilities. After implementing quality validation software based on the developed quality validation standards, the adequacy of the validation standards was demonstrated by testing using data from two-dimensional water supply facilities in some areas of Busan, Korea. This paper has great significance in that it has laid the foundation for reducing the time and manpower required for data quality inspection and improving data quality reliability by improving current quality validation standards and developing technologies that can automatically extract errors through software.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.50 no.4
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• pp.197-205
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• 2024

Objectives: To evaluate the effectiveness of decompression and various parameters that may affect volume change in cystic lesions. Patients and Methods: This retrospective study included patients who visited the Department of Oral and Maxillofacial Surgery at Ewha Womans University Medical Center between 2012 and 2022 for decompression of cystic lesions of the jaw. To measure volume changes, pre- and post-decompression cone-beam computed tomography was performed and reconstructed in three dimensions using Mimics 25.0 software (Materialise NV). A comparative analysis was performed based on sex, age, initial cyst volume, location, degree of cortical layer expansion, and pathologic diagnosis using the Mann-Whitney U and Kruskal-Wallis tests. Results: In all 20 cases, the duration of decompression was 7.84±3.35 months, and all patients successfully completed the decompression period without any complications. Significant differences were observed in the reduction rate and shrinkage speed based on the degree of cortical layer expansion. However, only the shrinkage speed (not the reduction rate) showed a significant difference with respect to the initial cyst volume. Significant differences were not observed based on sex, age, location, or pathologic diagnosis. Conclusion: Although the present study involved a small number of cases, the effectiveness of decompression was confirmed. In particular, 3D analysis overcame the shortcomings of previous studies of decompression and allowed earlier resection. Further studies with more patients are required to provide a rationale for these results and identify factors that influence decompression.

• Journal of the Korea Society of Computer and Information
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• v.29 no.9
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• pp.9-23
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• 2024

In this study, we propose a method to improve the classification accuracy of body mass index (BMI) estimation techniques based on three-dimensional gait data. In previous studies on BMI estimation techniques, the classification accuracy was only about 60%. In this study, we identify the reasons for the low BMI classification accuracy. According to our analysis, the reason is the use of the undersampling technique to address the class imbalance problem in the gait dataset. We propose applying oversampling instead of undersampling to solve the class imbalance issue. We also demonstrate the usefulness of anthropometric and spatiotemporal features in gait data-based BMI estimation techniques. Previous studies evaluated the usefulness of anthropometric and spatiotemporal features in the presence of undersampling techniques and reported that their combined use leads to lower BMI estimation performance than when using either feature alone. However, our results show that using both features together and applying an oversampling technique achieves state-of-the-art performance with 92.92% accuracy in the BMI estimation problem.

• Korean Journal of Applied Biomechanics
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• v.34 no.3
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• pp.133-144
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• 2024

Objective: Neuromuscular training may often result in an incomplete improvement of biomechanical risk factors associated with non-contact ACL injury. This study aimed to investigate the effects of reactive neuromuscular training (RNT) on dynamic postural control of the lower extremity during single-leg drop landing (SLDL) in comparison with conventional neuromuscular training (CNT). Method: Twenty-eight recreational female athletes were randomized to a CNT group (n=15) or a RNT group (n=13). All participants performed SLDL for three-dimensional motion analysis before and after CNT and RNT. The kinematic variable was calculated from before 50 milliseconds (ms) to after 100 ms initial contact: joint angle (degree). The kinetic variable was calculated from initial contact to after 100 ms: internal net joint moment (Nm/kg). Time series curve for normalized data was represented for group means and associated 95% confidence intervals. Effect sizes using Cohen's d was also calculated. Results: In within-group differences, the CNT group showed decreased knee flexion angle (0~100% of the task) and decreased knee valgus angle (0~50% of the task) after the intervention, whereas the RNT group showed increased hip flexion angle (0~14% of the task), decreased hip adduction angle (0~100% of the task), and decreased knee valgus angle (0~100% of the task) after the intervention. In between-group differences, the RNT group exhibited more hip flexion angle (0~49, 61~91% of the task), more knee flexion angle (0~100% of the task), and less knee valgus angle (0~55% of the task) than the CNT group. Conclusion: These findings suggest that both training intervention programs modified dynamic posture of the hip and knee during SLDL. Therefore, combining a neuromuscular training program with RNT technique would be more efficient for future injury prevention efforts.