• Journal of Microbiology and Biotechnology
• /
• v.21 no.2
• /
• pp.136-146
• /
• 2011

This study provides first-hand proteomic data on the survival strategy of Anabaena sp. PCC 7120 when subjected to long-term iron-starvation conditions. 2D-gel electrophoresis followed by MALDI-TOF/MS analysis of iron-deficient Anabaena revealed significant and reproducible alterations in ten proteins, of which six are associated with photosynthesis and respiration, three with the antioxidative defense system, and the last, hypothetical protein all1861, conceivably connected with iron homeostasis. Iron-starved Anabaena registered a reduction in growth, photosynthetic pigments, PSI, PSII, whole-chain electron transport, carbon and nitrogen fixation, and ATP and NADPH content. The kinetics of hypothetical protein all1861 expression, with no change in expression until day 3, maximum expression on the $7^{th}$ day, and a decline in expression from the $15^{th}$ day onward, coupled with in silico analysis, suggested its role in iron sequestration and homeostasis. Interestingly, the up-regulated FBP-aldolase, Mn/Fe-SOD, and all1861 all appear to assist the survival of Anabeana subjected to iron-starvation conditions. Furthermore, the $N_2$-fixation capabilities of the iron-starved Anabaena encourage us to recommend its application as a biofertilizer, particularly in iron-limited paddy soils.

• Journal of Environmental Impact Assessment
• /
• v.16 no.6
• /
• pp.421-432
• /
• 2007

To investigate temporal and spatial variations of hydrodynamics and environmental conditions in Gyeonggi Bay, including Han River estuary, numerical experiments were performed using 3-dimensional fine grid numerical ocean model. The model successfully reproduced the physical phenomena already known in Gyeonggi Bay where tide and fresh water discharge are dominant forcings. The calculated harmonic constants of tide and tidal current agreed well with those of observations at nine tide stations and two tidal current stations. Tidal asymmetries along the Yeomha Waterway, mainly caused by non-linear effect, were well reproduced and agreed well with observations. Time series of salinity at four stations(A, B, C and D) and horizontal distributions of monthly averaged salinity show that Gyodong and Seokmo Waterways play an important role in fresh water discharge into the Gyeonggi Bay rather than Yeomha Waterway.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.3
• /
• pp.607-611
• /
• 2004

Glycinin, one of the predominant storage proteins in soybeans, was examined as to whether it could be used as a calcium-binding mediator after chemical phosphorylation and enzymatic hydrolysis. Glycinin is composed of six subunits. One of the proglycinin subunits $(A_{la}B_{lb})$ was overexpressed in E. coli to obtain nonphosphorylated proteins with homogeneity. To investigate the enhanced calcium-binding properties of the phosphopeptides, the proglycinin was purified, phosphorylated, and hydrolyzed with trypsin. The proglycinin expressed in E. coli was purified by ammonium sulfate precipitation, ion-exchange chromatography, and cryoprecipitation. Chemical phosphorylation by sodium trimetaphosphate was performed to obtain phosphorylated proglycinin. After the phosphorylation, one-dimensional isoelectric focusing gel electroanalysis confirmed the phosphorylation of the proglycinin. The phosphorylated peptides were then hydrolyzed with trypsin, followed by a binding reaction with calcium chloride. The calcium-bound phosphopeptides were finally separated using immobilized metal $(Ca^{2+})$ chromatography. Consequently, a limited tryptic hydrolysate of the isolated phosphopeptides exhibited an enhanced calcium-binding ability, suggesting the potential of glycinin phosphopeptides as a calcium-binding mediator with greater availability.

• Journal of Korean Physical Therapy Science
• /
• v.20 no.1
• /
• pp.69-75
• /
• 2013

Background : The purpose of this study was to investigate the effects of the kinesiologic factors of gait on symmetric load. Methods : The subjects were consisted normal 33 persons (10 males and 23 females). The kinds of weight of the bag was 0kg, 5kg and 7kg. The kinesiologic factors of gait measured by three dimensional motion analysis system and callibration marker. Callibration was ASIS, hip greater trochanter, knee lateral epicondyle on sagittal plane, ankle lateral malleolus on sagittal plane, toe 5th phalange. The changes kinesiologic factor were analyzed using one way ANOVA with SPSS 21.0 package program. Results : The weight of the bag was statistical significance on change of hip joint and knee joint(p<.05). The weight of the bag was no significance on change of ankle joint(p>.05). The right and left of the lower limbs was no significant(p>.05). Conclusion : This research provides weight of bag for the gait. This study showed that symmetric load does affect kinesiologic factors of gait. This indicates that there is an interaction that plays a crucial roles in the weight of bag and kinesiologic factors of gait.

• Journal of Biosystems Engineering
• /
• v.31 no.4 s.117
• /
• pp.355-362
• /
• 2006

A one-dimensional heat conduction problem in cylindrical coordinate system was solved using Crank-Nicolson finite difference method to predicting the temperature distribution in rice storage bin with wall insulator. The model can simulate the grain temperatures in insulated round bins using the input data of initial grain temperature. ambient air temperature, wind velocity, solar radiation on a horizontal surface, and thermal properties of grain, bin wall, wall insulator, insulator cover, and air. Temperatures were collected at the bin center, 0.65m in radial direction from the center, and near the bin wall in 2.7m diameter bin filled with rough rice to depth of 3.0m were used to validate the simulation model. Grain temperatures predicted by the model were in very good agreement with the measured temperatures. The residual mean square error between measured and predicted grain temperatures at the bin center was $1.38^{\circ}C$.

• Steel and Composite Structures
• /
• v.34 no.5
• /
• pp.657-670
• /
• 2020

In this paper, vibration analysis of functionally graded nanoshell is studied based on the sinusoidal higher-order shear and normal deformation theory to account thickness stretching effect. To account size-dependency, Eringen nonlocal elasticity theory is used. For more accurate modeling the problem and corresponding numerical results, sinusoidal higher-order shear and normal deformation theory including out of plane normal strain is employed in this paper. The radial displacement is decomposed into three terms to show variation along the thickness direction. Governing differential equations of motion are derived using Hamilton's principle. It is assumed that the cylindrical shell is made of an arbitrary composition of metal and ceramic in which the local material properties are measured based on power law distribution. To justify trueness and necessity of this work, a comprehensive comparison with some lower order and lower dimension works and also some 3D works is presented. After presentation of comparative study, full numerical results are presented in terms of significant parameters of the problem such as small scale parameter, length to radius ratio, thickness to radius ratio, and number of modes.

• Journal of Satellite, Information and Communications
• /
• v.12 no.1
• /
• pp.107-113
• /
• 2017

Recently, digital signage is various changes and converging media. It is common to exhibit and guide focusing on information transfer by using the display. Such a method can not express variously changing digital signage. Therefore, we have to find a way to solve the problem of place and structure. In this paper, we experimented the new direction technique. Finally, we are going to propose acoustic direction technique using a microphone to maximize digital signage and intention of delivery by adding an sound reinforcement method to the visual representation.

• Journal of the Korean Society of Safety
• /
• v.33 no.5
• /
• pp.134-140
• /
• 2018

Scour in the downstream of hydraulic structures such as apron induces to collapse due to abruptly increasing rainfall and discharge in streams and reaches. This is because the forcible jet from overflowing is not sufficiently dissipated by existing energy dissipators, and it continues to sweep the bed materials during flood events. In this study, a second stilling basin was proposed as a countermeasure and the energy dissipation efficiency of this structure was analyzed using 3D-dimensional numerical analysis. First, results from previous research and hydraulic tests were used to verify the accuracy of the numerical model. It showed that the second stilling basin played a definite role in reducing the bottom velocity, comparing with diminishing the energy dissipation when numerical tests were conducted under scaled field conditions in Korea. This means that the second stilling basin can be a countermeasure against scour in downstream. If more efficiency analysis of the second stilling basin would be performed in terms of energy dissipator for various types of hydraulic jump, it would be an alternative solution to scouring issues.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.29 no.6
• /
• pp.14-26
• /
• 2015

This study has been conducted so as to analyse user's sensibility on lighting method, correlated color temperature and illumination by composing surface light source, which was projected onto a unit side of interior wall, ceiling and floor. 1) As an analyzed results of the sensibility images, it showed that the "snug & tender" value had got higher when the correlated color temperature got lower. And the "energetic, cheerful" value had got higher when the level of illuminance got lower. Furthermore, the "unusual, unique" showed higher value on the illuminated floor circumstance. Finally, the higher correlated color temperature had been, "energetic, cheerful" value also got higher. 2) As a result of multi-regression analysis, it was found that 3000K and 100lx had the biggest influence on 'snug' image while 5,500K, 500lx had the biggest influence on 'energetic' image. In addition, it was found that the illuminated floor had a big influence on 'unusual' image while 500lx had the biggest influence on 'refined' image.

• Journal of the Korean Society of Combustion
• /
• v.13 no.3
• /
• pp.17-23
• /
• 2008

The spray combustion and spray cooling depends on droplet evaporation. So, evaporation model for spray has been requested and lots of investigation has been done and various reliable models have been developed also for last few decades. In the present study, One dimensional quasi-steady spherically symmetric droplet evaporation model for micro-gravity is developed. The gas phase was assumed as steady state and the thermophysical properties are calculated as a function of temperature, pressure and composition and the properties used in the model was validated by NIST web data and overall evaporation history results was compared with experimental results by Nomura and Qasim and gave satisfactory agreements. Through this model, diverse phenomenon was investigated, especially regarding the effects of ambient pressure and temperature. The effects of pressure for the droplet evaporation time were studied. The high pressure increased the droplet surface temperature and made effect on the evaporation time depend on atmospheric temperature. The role of the ambient temperature was investigated and explained. The basic investigation for the evaporation process according to variation of droplet diameter and surface temperature were also investigated and the well-known phenomena, like D-square-law, were reported, too.