• BMB Reports
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• v.37 no.3
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• pp.298-303
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• 2004

In general, a SYPRO Ruby dye is well known as a sensitive fluorescence-based method for detecting proteins by one-or two-dimensional SDS-PAGE (1-DE or 2-DE). Based on the SYPRO Ruby dye system, the combined two-dimensional fibrin zymography (2-D FZ) with SYPRO Ruby staining was newly developed to identify the Bacillus sp. proteases. Namely, complex protein mixtures from Bacillus sp. DJ-4, which were screened from Doen-Jang (Korean traditional fermented food), showed activity on the zymogram gel. The gel spots on the SYPRO Ruby gel, which corresponded to the active spots showing on the 2-D FZ gel, were analyzed by a matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis. Five intracellular fibrinolytic enzymes of Bacillus sp. DJ-4 were detected through 2-D FZ. The gel spots on the SYPRO Ruby dye stained 2-D gel corresponding to 2-D FZ were then analyzed by MALID TOF MS. Three of the five gel spots proved to be quite similar to the ATP-dependent protease, extracellular neutral metalloprotease, and protease of Bacillus subtilis. Also, the extracellular proteases of Bacillus sp. DJ-4 employing this combined system were identified on three gels (e.g., casein, fibrin, and gelatin) and the proteolytic maps were established. This combined system of 2-D zymography and SYPRO Ruby dye should be useful for searching the specific protease from complex protein mixtures of many other sources (e.g., yeast and cancer cell lines).

• Journal of the Korean Society of Marine Environment & Safety
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• v.18 no.5
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• pp.388-397
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• 2012

In this study, 3D-NIT(3-Dimensional Numerical Irregular wave Tank) model in which regular wave as well as stable irregular wave can be generated in 3-dimensional numerical irregular wave tank was proposed. To verify validity, the following steps need to be conducted: 1) comparative analysis between calculated waveforms and targeted waveforms at the wave generating point, 2) comparative analysis with the existing experimental values of overtopping volume estimated, targeting shore protection structures installed on a slope bed, 3) comparison with the existing numerical and hydraulic experimental results through application in the analysis on the wave deformation by structures and wave force acting on the vertical cylindrical structures. Based on the results, characteristics of the breaking wave forces according to incident waves and interval distance of structures were identified through application of 3D-NIT model in the analysis on the breaking wave forces acting on the cylindrical structures installed on a slope bed, and reflection and overtopping was reviewed through application in the special breakwaters on the domestic fields. The numerical results obtained the 3D-NIT model are in good agreement with experimental results, and its applicaion to the complex-shpaed coastal structures is verified.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.11
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• pp.1299-1307
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• 2014

In this work, an appropriate analysis model of a precise foot sensor with low detection pressure capability under a low range of variation in the dimensional variables was proposed. With a simple two-dimensional model, it was found that a remarkably high error level sometimes occurred between the analysis and experimental results. In order to overcome the error and improve the performance, a three-dimensional model was introduced, and the detection pressure and sensor characteristics were compared with those of the experimental results, which showed its enhanced performance with less error and higher precision.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.1
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• pp.107-116
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• 2010

The existing cadastral record cannot meet various and changing demands on land information, improve user convenience, and raise administrative efficiency. In addition, three-dimensional parcels, or spatial objects about three-dimensional space cannot be registered in the conventional cadastral record. The limitation of cadastral information based on two dimensions is quite stressing the necessity of three-dimensional cadastral record. The purpose of this study is to develop new limns of cadastral record model in order. In register three-dimensional positions and right relations of land and buildings. This study examined land cases where space was being three-dimensionally used. As the result, cadastral record models both separated by steps and integrated were developed, which can contain matters of land, buildings, and right registration about three-dimensional land parcels. Also, this study suggested a method where a building can be separately registered according to it's the superficies division.

• Journal of KIBIM
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• v.8 no.2
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• pp.41-50
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• 2018

The current study focused on the utilization of building information modeling (BIM) data in steel-frame structures, which help to reduce project durations because they employ prefabricated structural members that are assembled on-site. In addition, a business process model was proposed using BIM data collected during the preconstruction, structural steel fabrication, and on-site construction phases of an actual steel-frame project. The ultimate expectation is that BIM data support at each phase, as well as the increased understanding among project participants, will result in an increase in project management productivity. The results from the current study are summarized as follows: To implement a BIM capable of application to steel-frame projects and data utilization, existing theories were studied to develop the construction project steps, both generally into the preconstruction (A1), steel fabrication (A2), and on-site construction phases, (A3) and specifically into 19 BIM-applicable phases. Based on the derived BIM-applicable phases, the model elements of the BIM object were identified, and the shortcomings of existing steel-frame projects were ameliorated, resulting in an improved data flow model. Moreover, for the proposed BIM data flow to progress efficiently, the BIM specialist needs to be well-acquainted with the phase-specific three-dimensional (3D) model output, and the infrastructure to construct an error-free 3D model must be provided. Based on the actual construction example, the BIM data utilized steel-frame projects - via production reports, clash checks, two-dimensional (2D) drawings, four-dimensional (4D) simulations, and 3D scanning - to make cooperation and communication among participants easier.

• Journal of Korean Neurosurgical Society
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• v.67 no.2
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• pp.166-176
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• 2024

Objective : Three-dimensional (3D) printing in vascular surgery is trending and is useful for the visualisation of intracranial aneurysms for both surgeons and trainees. The 3D models give the surgeon time to practice before hand and plan the surgery accordingly. The aim of this study was to examine the effect of preoperative planning with 3D printing models of aneurysms in terms of surgical time and patient outcomes. Methods : Forty patients were prospectively enrolled in this study and divided into two groups : groups I and II. In group I, only the angiograms were studied before surgery. Solid 3D modelling was performed only for group II before the operation and was studied accordingly. All surgeries were performed by the same senior vascular neurosurgeon. Demographic data, surgical data, both preoperative and postoperative modified Rankin scale (mRS) scores, and Glasgow outcome scores (GOS) were evaluated. Results : The average time of surgery was shorter in group II, and the difference was statistically significant between the two groups (p<0.001). However, no major differences were found for the GOS, hospitalisation time, or mRS. Conclusion : This study is the first prospective study of the utility of 3D aneurysm models. We show that 3D models are useful in surgery preparation. In the near future, these models will be used widely to educate trainees and pre-plan surgical options for senior surgeons.

• Journal of the Ergonomics Society of Korea
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• v.23 no.2
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• pp.25-36
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• 2004

The effects of task characteristics on the relative efficiency of visual display dimension were studied using a simulated tele-robotic task. Through a conventional method of task analysis. the tele-robotic task was divided into two categories: the task element requiring focused attention (FA task) and the task element requiring global attention (CA task). Time-ta-completion data were collected for a total of 120 trials involving 10 participants. For the CA task. there was no significant difference between the multiple two-dimensional (20) display and the three-dimensional (3D) monocular display. For the FA task. however. the multiple 20 display was superior to the 3D monocular display. The results suggest that the characteristics of a given task have a considerable effect on the choice of display dimensionality and the multiple 3D display is better for human operators to effectively judge depth if the task requires frequent use of focused attention.

• Smart Structures and Systems
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• v.13 no.1
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• pp.111-135
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• 2014

Based on the Reissner mixed variational theorem (RMVT), finite rectangular layer methods (FRLMs) are developed for the three-dimensional (3D) linear buckling analysis of simply-supported, fiber-reinforced composite material (FRCM) and functionally graded material (FGM) sandwich plates subjected to bi-axial compressive loads. In this work, the material properties of the FGM layers are assumed to obey the power-law distributions of the volume fractions of the constituents through the thickness, and the plate is divided into a number of finite rectangular layers, in which the trigonometric functions and Lagrange polynomials are used to interpolate the in- and out-of-plane variations of the field variables of each individual layer, respectively, and an h-refinement process is adopted to yield the convergent solutions. The accuracy and convergence of the RMVT-based FRLMs with various orders used for expansions of each field variables through the thickness are assessed by comparing their solutions with the exact 3D and accurate two-dimensional ones available in the literature.

• Steel and Composite Structures
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• v.27 no.6
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• pp.789-801
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• 2018

A weak-form formulation of finite annular prism methods (FAPM) based on Reissner's mixed variational theorem (RMVT), is developed for the quasi three-dimensional (3D) static analysis of two-directional functionally graded (FG) circular plates with various boundary conditions and under mechanical loads. The material properties of the circular plate are assumed to obey either a two-directional power-law distribution of the volume fractions of the constituents through the radial-thickness surface or an exponential function distribution varying doubly exponentially through it. These FAPM solutions of the loaded FG circular plates with both simply-supported and clamped edges are in excellent agreement with the solutions obtained using the 3D analytical approach and two-dimensional advanced plate theories available in the literature.

• International Journal of Internet, Broadcasting and Communication
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• v.11 no.1
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• pp.54-59
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• 2019

The parametrization of the surface plays an important role in the 3D computer graphics and computer vision. Especially, it is essential in 3D mesh editing that manipulates the position of 3D objects on a computer monitor. Because computer monitors are two-dimensional, while vertices of objects that actually want to change are in a three-dimensional virtual space. In this study, we propose a new type of parametrization method that takes advantage of the special properties of circles that are common to local parameterization. Experimental results show that our parameterization method is more efficient in terms of time and space than the standard parameterization method that was designed by Floater et al.