• Applied Microscopy
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• v.48 no.3
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• pp.55-61
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• 2018

The synaptic vesicle is a specialized structure in presynaptic terminals that stores various neurotransmitters. The actin filament has been proposed for playing an important role in mobilizing synaptic vesicles. To understand the role of actin filament on synaptic vesicle pooling, we characterized synaptic vesicles and actin filament after treatment of brain-derived neurotrophic factor (BDNF) or Latrunculin A on primary cultured neuron from rat embryo hippocampus. Western blots revealed that BDNF treatment increased the expression of synapsin I protein, but Latrunculin A treatment decreased the synapsin I protein expression. The increased expression of synapsin I after BDNF disappeared by the treatment of Latrunculin A. Three-dimensional (3D) tomography of synapse showed that more synaptic vesicles localized near the active zone and total number of synaptic vesicles increased after treatment of BDNF. But the number of synaptic vesicle was 2.5-fold reduced in presynaptic terminals and the loss of filamentous network was observed after Latrunculin A application. The treatment of Latruculin A after preincubation of BDNF group showed that synaptic vesicle number was similar to that of control group, but filamentous structures were not restored. These data suggest that the actin filament plays a significant role in synaptic vesicles pooling in presynaptic terminals.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.1
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• pp.43-52
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• 2009

LIDAR data, as the source of the 3D information of buildings, are used many modeling fields such as three-dimensional city models in urban planning and the visibility analysis of buildings. This study suggests a methodology, that is characterized by combining image-based and point-based process, for minimizing the user's intervention and automatically extracting building boundary only using the LIDAR data. Image processing methodology is firstly used to separate building and non-building regions from LIDAR data. Moreover, building regions are then classified main roof into remaining parts by the statistical analysis of height values, and the remaining parts are processed separately. Through the experimental results of study areas which exist many types of buildings, for example, apartment-type, stair-type, complex-type, etc. Approximately 90% building boundaries are automatically extracted by the proposed methodology.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.5
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• pp.395-404
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• 2012

This paper presents computations of the dynamic derivatives of three dimensional flight vehicle configurations using CFD. The pitch dynamic derivatives are computed from the pitch sinusoidal motion, while the roll damping is computed based on steady state calculation using a non-inertial frame method. The Basic Finner and the SDM(Standard Dynamic Model) are chosen for the benchmark tests against other numerical and experimental results. For the flow calculations, a 3-D Euler solver that can be run both on the non-inertial frame and on the inertial frame is developed. A dual-time stepping method is applied for the unsteady time accurate simulations. A good agreement of pitch-roll dynamic derivatives with previously published numerical results and the experimental results is observed.

• Physical Therapy Korea
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• v.27 no.3
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• pp.199-205
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• 2020

Background: The classification of foot type can be commonly determined by the height of the media longitudinal arch. The normalized arch height (NAH) is defined as the ratio of navicular or instep heights to the foot length or instep length. Objects: This study investigated the relationships among foot characteristics, such as foot length (FL), instep length (IL), navicular height (NH), and instep height (IH), in Korean young adults. Also, the distribution of foot type based on calculated NAH was assessed. Methods: Three-dimensional foot scanning data of young adults aged 20 to 39 years (total: 1,978; 974 male, 1,004 female) were obtained from the Korea Technology Standards Institute, and used for analyses. NAH was calculated as the following: NH/FL, IH/FL, IH/IL, NH/IL. Spearman's rank order correlation was used to identify correlations among variables. The Mann-Whitney U-test and chi-square test were used to compare the sex differences in foot characteristics and distribution of foot type. Results: FL and IL showed a very high correlation (r = 0.94). The correlations between FL or IL and IH (r = 0.50-0.57) were greater than those between FL or IL and NH (r = 0.23-0.72). Males had significantly larger values than females (p < 0.001), and the frequency of pes planus was significantly higher in females than in males (χ² = 50.09, p < 0.001). Based on the IH/IL index, the neutral foot, pes planus and pes cavus distributed by 16%, 78%, and 6% respectively. Conclusion: Our results on foot arch distribution could be used as basic data in clinical or footwear fields, and our data on differences in arch structure according to sex may facilitate understanding of why injury to the lower limbs differs between males and females.

• Journal of the Korean Society for Railway
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• v.19 no.1
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• pp.67-76
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• 2016

The asphalt-concrete trackbed system has many advantages in terms of maintenance and economics. However, methods to investigate practical use corresponding to the development of the trackbed system must be developed. The primary objective of this study was to evaluate the dynamic performance of the asphalt system in accordance with both the elastic and viscoelastic material characteristics and design thickness of the asphalt trackbed. More specifically, in order to reduce the uncertainty error of the Finite Element(FE) model, a three-dimensional full scale FE model was developed and then the infinite foundation model was considered. Finally, to compare the condition of viscoelastic materials, performance evaluation of the asphalt-concrete trackbed system was used to deal with the dynamic amplification factors; numerical results using isotropic-elastic materials in the FE analysis are presented.

• Journal of Integrative Natural Science
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• v.3 no.2
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• pp.72-77
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• 2010

Alginate lyases, also known as alginases or alginate depolymerases, catalyze the degradation of alginate by a ${\beta}$-elimination mechanism that has yet to be fully elucidated. Alginate is a copolymer of ${\alpha}$-L-guluronate (G) and its C5 epimer ${\beta}$-D-mannuronate (M), arranged as homopolymeric G blocks, M blocks, alternating GM or random heteropolymeric G/M stretches. Almost all alginate lyases depolymerize alginate in an endolytical fashion via a ${\beta}$-elimination reaction. The alginate lyase Atu3025 from Agrobacterium tumefaciens strain C58, consisting of 776 amino-acid residues, is a novel exotype alginate lyase classified into polysaccharide lyase family 15. Till now there is no crystal structure available for this class of proteins. Since there is no template with high sequence identity, three-dimensional coordinates for exotype alginate lyase (PL 15 family) were determined using modeling methods (Comparitive modeling and Fold recognition). The structures were modeled using the X-ray coordinates from Heparinase protein family (PDB code: 3E7J). This enzyme (Atu3025) displays enzymatic activity for both poly-M and poly-G alginate. Since poly-M is widespread; docking of a tri-mannuronate against the modeled structure was performed. We identified some of those residues which are crucial for lyase activity. The results from this study should guide future mutagenesis studies and also provides a starting point for further proceedings.

• Journal of Korean Society for Geospatial Information Science
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• v.11 no.1 s.24
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• pp.71-75
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• 2003

The highway is greatly changed by the constant economic growth for a long times the traffic situation such as the large volumes and the performance vehicles, the performance enlargement of vehicles, the high speedization, etc., due to growth economic. A study of an optimal route selection model is researched over late 1980s by development of computer and GSIS, and consisted including research about the optimal route that uses digital terrain model in domestic such as the earth volume calculations, the mass curve output and the automation system construction. Lately, the study of the driving simulation of the highway and the virtual reality using VGIS(Virtual Geographic Information System) is researched. This study shows when the alternative highway selection considered surrounding facilities, development plan and according to estimate amount of traffic and the additional possibility of view analysis and environment effect analysis element will study through 3D simulation method.

• Journal of Digital Convergence
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• v.16 no.10
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• pp.453-463
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• 2018

This study investigated the effects of an Augmented Reality(AR) implemented book on flow of reading, interest in history, and acquisition of history knowledge. Perceptions of AR infused books were investigated as well. Researchers provided a history book implementing AR and the same book without any AR content respectively to an experiment group(n=15) and a control group(n=15) composed of $3^{rd}$ and $4^{th}$ grade elementary school children. Results indicate that AR implemented reading had a positive effect on the flow of reading and interest in history, but not on acquisition of history knowledge. Also, AR-based contents were attractive to learners due to its amusing characters, sound, realistic visual motions, and vivid three-dimensional effects. Lastly, students preferred amusing interesting characters, lengthier animations and subtitles, and AR that could be seen without holding smart devices for a long while.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.34 no.6
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• pp.1911-1918
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• 2014

The increase of input resources, such as labor and equipment, in a construction project causes workspace interference between activities and it influences on the productivity and quality of construction activities. To solve this problem, many studies related to the workspace interference have been performed, however they verified the workspace concerning with only the geometric location of activities or generated the shape of workspace by a whole object concept not separated units of detailed operations. It is difficult for project manager to reasonably analyze the workspace conflict, because the size of workspace cannot reflect the characteristics of an activity and input time of a resource. This paper presents a methodology that can generate three-dimensional models in order to optimize the workspace shape and size by considering with the characteristics of each activity and input time of each resource. The suggested method can be used for the active BIM system that optimizes the workspace conflict without additional construction duration and for the searching algorithm of optimized moving path for construction equipment.

• Interaction and multiscale mechanics
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• v.3 no.1
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• pp.55-79
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• 2010

The effect of soil-structure interaction on a single-storey, two-bay space frame resting on a pile group embedded in the cohesive soil (clay) with flexible cap is examined in this paper. For this purpose, a more rational approach is resorted to using the finite element analysis with realistic assumptions. Initially, a 3-D FEA is carried out independently for the frame on the premise of fixed column bases in which members of the superstructure are discretized using the 20-node isoparametric continuum elements. Later, a model is worked out separately for the pile foundation, by using the beam elements, plate elements and spring elements to model the pile, pile cap and soil, respectively. The stiffness obtained for the foundation is used in the interaction analysis of the frame to quantify the effect of soil-structure interaction on the response of the superstructure. In the parametric study using the substructure approach (uncoupled analysis), the effects of pile spacing, pile configuration, and pile diameter of the pile group on the response of superstructure are evaluated. The responses of the superstructure considered include the displacement at top of the frame and moments in the columns. The effect of soil-structure interaction is found to be quite significant for the type of foundation considered in the study. Fair agreement is observed between the results obtained herein using the simplified models for the pile foundation and those existing in the literature based on a complete three dimensional analysis of the building frame - pile foundation - soil system.