• Journal of Applied Biological Chemistry
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• v.51 no.3
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• pp.88-94
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• 2008

Three different protein extraction methods-phenol extraction, trichloroacetic acid (TCA) precipitation, and desalting/TCA precipitation-were compared to determine the optimal reproducible high resolution 2-dimensional (2-D) electrophoresis for each chungkugjang, doenjang, and kochujang samples. The soluble proteins from Chungkugjang extracted by phenol were separated with high reproducibility and resolution, and gained 1.75- to 3-fold more protein spots on 2-D gel than those from the other methods. On the contrary, the extracted proteins from doenjang and kochujang treated by desalting/TCA precipitation method showed about 1.5- to 3.3-fold more protein spots on 2-D gel. Using the established methods, the changes in the protein profiles of the fermented soy foods were monitored during the fermentation period by 2-DE. One of the major proteins in soy, $\beta$-conglycinin $\alpha$-subuint, and some proteins with unknown functions were localized on 2-D gel as the protease-resistant proteins throughout the fermentation period of doenjang. Changes in the protein profile monitored by the established methods can provide basic information on unfolding the mechanisms of the generation of biofunctional activity in the fermented soy foods.

• 한국가시화정보학회:학술대회논문집
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• 2007.11a
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• pp.109-113
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• 2007

Micro-PIV is a well-known method for measurement of two- dimensional, two-component velocity in the microfluidic devices. Lots of the micro fluidic devices generate three-dimensional flow and 3D measurement of velocity is helpful to understand the physics of micro flow phenomena. In this study, we developed new micro 3D measurement method by applying 2-frame PTV in stereoscopic micro system. In this study, we did the validation study of SMPTV by using the simulated flow model to verify the accuracy and the feasibility of measurement and compared with SMPIV method. The results showed that SMPTV provides better spatial resolution and measurement accuracy than SMPIV method.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2001.10a
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• pp.245-252
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• 2001

The ultimate strength testing of a two-story, single-bay, and sway allowed space steel frame was performed. Considering a majority of large-scale frame tests in the past, only two-dimensional frames were experimentally studied. Therefore, three-dimensional experiment is needed to extend the knowledge of this field. The steel frame subjected to non-proportional vertical and horizontal load was tested. The load-displacement curve of the test frame is provided. The experiment results are useful for verification of the three-dimensional numerical analysis. The results obtained from 3D non-linear analysis using ABAQUS were compared with experimental data.

• Journal of Ocean Engineering and Technology
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• v.4 no.1
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• pp.55-61
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• 1990

For the tracking of three dimensional free surface motions, a method referred to as the Volume of Fluid(VOF) algorithm is extended. In order to calculate the slope of three dimensional free surface which is the most important for the advection algorithm that decides the amount of fluid from cell to cell and for the application of free surface boundary condition, a simple method utilizing two dimensional slope informations is introduced. The extended algroithm is tested by demonstrating the simulation of a propagating sinusoidal wave through the channel whose width changes abruptly.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.72-83
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• 2009

The purpose of this study is to examine the effects of a degree of discretization in the direction of longitudinal dam axis on the results of three dimensional fill dam dynamic analysis. In this study, the three dimensional dynamic analyses of the existing 'H' dam which is modeled with a different degree of discretization were carried out. From these results, the fundamental frequency of the dam and the responses at the dam crest such as acceleration and settlement were compared and analyzed. It was concluded that the size of finite element discretized in the direction of the longitudinal axis mush be smaller than 1/8 of dam length in order to obtain the reasonable fundamental frequency and response of acceleration and mush be smaller than 1/10 in order to obtain the reasonable settlement behaviors from the three dimensional dynamic analysis of the fill dam.

• Korean Journal of Computational Design and Engineering
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• v.12 no.1
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• pp.39-49
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• 2007

Recently three-dimensional (3-D) die design and production process has been widely introduced into the tooling shops of automotive manufacturers to reduce time-to-production of brand-new automobiles. 3-D solid models created in CAD systems are used not only for various simulations for design verification, but also for NC tool path generation to machine dies and their Styrofoam patterns. However, a lot of time and cost will be required to build solid models for dies if designers use only the generalized modeling capabilities of commercial 3-D CAD systems. To solve this problem, it is necessary to customize 3-D CAD system for the specific die design and manufacturing process. This paper describes a dedicated 3-D CAD system based on Unigraphics for die design for automotive body panels. The system provides not only 3-D design capabilities, but also standard part libraries, to enhance design productivity. The design process modeling technology has been introduced to facilitate redesign of the die for the modified panel. By introducing this system, dies can be designed more rapidly in the 3-D space, and their solid data can be directly transferred to CAM tools for NC tool path generation and simulation tools for virtual manufacturing.

• Journal of Technologic Dentistry
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• v.43 no.1
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• pp.13-18
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• 2021

Purpose: This study aimed to compare the flexural properties and perform the Weibull analysis of photo-curing three-dimensional (3D) printing resin. Methods: Photo-curing temporary resin (3D polymer) was used as a printing resin. Specimens (65 × 10 × 3.3 ㎣) were prepared following the ISO 20975-1 guidelines and according to the different printing orientations using a digital light processing 3D printer (D2 120; Dentium). The flexural strength (FS), flexural modulus, and work of fracture (WOF) were measured using a universal testing machine (Instron 3344; Instron) at a crosshead speed of 5 mm/min. Results: In this study, the 0° orientation exhibited higher FS and WOF than the 45° orientation. Significant differences were found among the printing orientations (p<0.05). Specimens printed at the 0° orientation were the most accurate. In the Weibull analysis, 0° showed the greatest Weibull modulus (m), which represents a higher reliability. Conclusion: 3D printing should be selected and used by considering flexural properties, size accuracy, and reliability.

• Korean Chemical Engineering Research
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• v.55 no.5
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• pp.698-703
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• 2017

Bubble flow characteristics in fluidized beds were analyzed by CPFD simulation. A fluidized bed, which had the size of $0.3m-ID{\times}2.4m-high$, was modeled by commercial CPFD $Barracuda^{(R)}$. Properties of bed material were $d_p=150{\mu}m$, ${\rho}_p=2,330kg/m^3$, and $U_{mf}=0.02m/s$. Gas was uniformly distributed and the range of superficial gas velocity was 0.07 to 0.16 m/s. Two other geometries were modeled. The first was a three-dimensional model, and the other was a two-dimensional model of $0.01m{\times}0.3m{\times}2.4m$. Bubble size and rising velocity were simulated by axial and radial position according to superficial gas velocity. In the case of three-dimensional model, simulated bubble rising velocity was different from correlations, because there was zigzag motion in bubble flow, and bubble detection was duplicated. To exclude zigzag motion of bubble flow, bubble rising velocity was simulated in the two-dimensional model and compared to the result from three-dimensional model.

• Korean Institute of Interior Design Journal
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• v.16 no.5
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• pp.38-46
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• 2007

In the late 1970s, the method of quantitative and scientific space structural analysis based on graph theory was introduced to the process of space design, which arranges design and functional elements, as relying heavily on intuition could produce errors due to unverified experiences and prejudices of the designer. As the method of space analysis is complex and hard to express visually and requires repetitive operations, it was discussed theoretically only. However, with the development of computer performance and graphic in recent years, visualization became possible. But the method of visual structural analysis of space is at the level of two dimensions and it is not easy to get accurate data when it is applied to limited three dimensional space such as an interior space. For the visual structural analysis of space, this study presents 4 indices including visibility volume level, pure visibility connection frequency, effective visibility connection frequency, and path visibility connection frequency. This study also presents space division using three dimensional arrangement rather than the existing vector operation method and raytracing algorithm at the lattice constant. Based on this, an analysis tool for the visible regions of three dimensional space that is capable of evaluating at multiple points by using three dimensional game engine and presentation tool that allows the analyzer to interpret the data effectively is made. It is applied to 2 prototype models by displacing Z axis, and the results are compared with UCL Depthmap to verify the validity of data and evaluate its usefulness as a multidimensional, multi-view space analysis tool.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.11B
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• pp.1311-1319
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• 2009

Microscopic specimen was captured by an integral imaging microscope and displayed as a three-dimensional image by an integral imaging display system. We applied the generalized relationship between pickup and display using two different lens arrays to our integral imaging microscope and display system. In order to display three-dimensional microscopic image, scaling of the captured elemental images is required. We analyzed the effect of the scaling coefficient in terms of the distortion of the displayed three-dimensional image and the loss of the captured elemental images. In our experiment, microscopic specimen is picked up by an integral imaging microscope having $125{\mu}m$ elemental lens pitch and displayed as three-dimensional image by an integral imaging display system having 1mm elemental lens pitch. The scaling coefficient was chosen to minimize the elemental image loss.