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Analysis of Chemical Composition and in vitro Anti-oxidant Properties of Extracts from Sea Buckthorn (Hippophae rhamnoides) (비타민나무(Sea Buckthorn, Hippophae rhamnoides) 추출물의 이화학적 성분 분석과 항산화 활성효과)

  • Kim, Kyung-Min;Park, Min-Hee;Kim, Kyung-Hee;Im, Sang-Hyun;Park, Yoo-Hwa;Kim, Young-Nam
    • Journal of Applied Biological Chemistry
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    • v.52 no.2
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    • pp.58-64
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    • 2009
  • Total polyphenol contents and antioxidative activity of water and ethanol extracts from Sea Buckthorn (Hippophae rhamnoides) leaves, branches (bough and twig) and roots were analyzed. The level of crude ash and crude protein in water extract of leaves, branches (bough and twig), and roots were shown to be a bit higher than ethanol extracts. Especially crude protein contents from water extract of leaves, bough, twig, and roots were 14.90, 18.60, 18.03, and 16.61% respectively. Total polyphenol content of ethanol extracts of all parts of Sea buckthorn was ranged from 106.33${\pm}$2.32 ${\mu}g/g$ to 147.78${\pm}$3.06 ${\mu}g/g$ showing higher amount than water extracts. To investigate antioxidative activity of Sea buckthorn, DPPH free radical scavenging activity, hydroxy radical scavenging activity, and SOD-like activity were analyzed. The results showed that the antioxidative activity of ethanol extracts was relatively higher than water extracts. The $IC_{50}$ values for DPPH free radical scavenging activity was ranged from 23.58${\pm}$0.84 ${\mu}g/mL$ to 59.35${\pm}$1.69 ${\mu}g/mL$. Compared to 68.85${\pm}$1.44% of SOD-like activity from L-ascorbic acid used as a control, the ethanol extract of Sea buckthorn branches showed relatively strong activity of 35.03${\pm}$2.33%. The highest hydroxy radical scavenging activity was shown as 66.12${\pm}$8.73% from ethanol extract of Sea buckthorn roots which was similar value to 72.47${\pm}$2.83% of L-ascorbic acid.

Effects of Gardenia jasminoides Ellis Peel Extract in Namhae Korea on the Bioactivity Compounds and Lipid Peroxidation Inhibition Activity (남해산 치자(Gardenia jasminoides Ellis fructus) 껍질의 생리활성 및 지질과산화 저해 활성에 미치는 영향)

  • Jin, Dong-Hyeok;Oh, Da-Young;Kang, Dong-Soo;Lee, Young-Geun;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.1
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    • pp.263-272
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    • 2018
  • The object of this study was to measure the bioactivity and lipid peroxidation inhibition activity of peel from Gardenia jasminoides Ellis fructus (GJE) in Namhae Korea. The amount of phytic acid was also determined. Extraction was performed using three solvents, CM (choloform:methanol, 2:1, v/v), n-butanol and 70% ethanol. To investigate by the solvent extract of total phenol content and value as a functional food ingredient of GJE peel through nitrogen oxide scavenging activity, antioxidant activity, reducing power and lipid peroxidation inhibition were performed. The bioactivities of the extract solvents increased significantly with increasing concentrations (0.2, 0.4, 0.6 mg/mL, p<0.05). The total phenol contents of GJE peel extracts were highest in CM ($39.74{\pm}0.15mg\;CAE/g$) extract. The order of total phenol contents, antioxidant activity and reducing power of the solvents in the GJE peel were the same, in the analysis of nitrogen oxides scavenging activity and lipid peroxidation inhibition, it was confirmed the results were inconsistent. As a result, the GJE peel showed excellent bioactivities. Considering the extraction yield and various physiological activities, it is considered that efficiency is better when extracted from CM and 70% ethanol extracts.

The effects of stepping in place tempo and roundhouse kick types on response time in taekwondo (태권도에서 제자리딛기 템포와 돌려차기 유형이 응답시간에 미치는 영향)

  • Lee, Jong-Hwa;Song, Young-Hoon
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.4
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    • pp.870-877
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    • 2020
  • The purpose of this study is to compare and analyze the effects of stepping-in-place tempo and roundhouse kick types on response time. Fifteen males participated in this experiment. All have over ten years of experience and hold a forth dan(degree) black belt in taekwondo. The task is when the participants are doing stepping in place they respond to the light stimulus as fast as they can do roundhouse kick. Five different stepping in place tempos (100, 120, 140, 160, and 180 bpm) and four different types of roundhouse kick(front leg body roundhouse kick, front leg head roundhouse kick, back leg body roundhouse kick, and back leg head roundhouse kick) were used. Three measurements were taken for each of the different combinations of conditions for a total of 60 measurements. For data analysis, two-way ANOVA with repeated measures was used and pair-wise comparisons were performed using bonferroni statistics. The results show that there was significant difference interaction effect between stepping in place tempo and roundhouse kick type in the response time. And, there were significant difference in main effect of response time in accordance with stepping in place tempo and roundhouse kick type. The response time of roundhouse kick was the fastest at 160 bpm of stepping in place tempo, but there was no significant difference between 140 and 160 bpm. Front leg body roundhouse kick was the fastest. And, the response time was the fastest when front leg body roundhouse kicked at 140 bpm of stepping in place tempo. Stepping in place tempo between 140 and 160 bpm is the most effective to optimize the response time. And, More effective response time was front leg roundhouse kick as compared with back leg roundhouse kick and, body roundhouse kick as compared with head roundhouse kick. The findings in this study will provide useful information for performance improvement and will help with strategy for taekwondo competition.

Return to Sports Activity After Anterior Cruciate Ligament Reconstruction (전방십자인대 재건술 후 스포츠 활동 복귀)

  • Park, Jong-Hyuk;Lee, Jun-Mo;Bae, Hyun-Kyung;Im, Jong-Han;Lee, Ju-Hong
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.8 no.2
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    • pp.95-101
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    • 2009
  • Purpose: The purpose of this study was to determine how many of our patients who had had anterior cruciate ligament (ACL) reconstruction returned to their previous levels of sports and to identify why not doing so. Material and Methods: We evaluated 43 knees with a minimum of two years follow-up among whom undergoing 109 ACL reconstruction from September 2003. The mean age of patients was 26 years (17 to 45 years) Clinical results was evaluated according to the Lysholm Knee scores, Tegner activity score, subjective IKDC questionnaires, objective IKDC evaluation form and KT-1000 arthrometer. Results: The overall clinical results demonstrated significant improvement at the final follow-up comparing to preoperative data (P<0.05). Among 43 patients, 25 patients (58%) returned to their previous level of sports and 18 patients (42%) did not. Of whom, 11 patients (61%) were not able to return to sports due to fear to re-injury and 7 patients (39%) suffered from instability and pain. The differences in the Lysholm Scores, KT-1000 arthrometer and subjective IKDC in the 3 groups of patients by ANOVA test were found to be statistically significant (P<0.001). Although there is no difference in objective IKDC and KT-1000 arthrometer, the difference was observed in Tegner activities, subjective IKDC obtained by those who returned to sport and those who had fear to reinjury (P<0.001). Conclusion: This study showed that a quater of patients could not return to their previous level of sport after ACL reconstruction because of fear to reinjury. We thought that not only improving the technique of ACL reconstruction and systemic rehabilitation but also considering psychological factor of patients could allow more patients to return to the previous level of sports.

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Comparison of dietary behavior, changes of diet, and food intake between 40~59 years old subjects living in urban and rural areas in Lao PDR (라오스 도시·농촌 지역별 40~59세 주민들의 식행동, 식생활변화 및 식품섭취 비교 연구)

  • Kim, Ji Yeon;Yi, Kyungock;Kang, Minah;Kang, Younhee;Lee, Gunjeong;Kim, Harris Hyun-soo;Hansana, Visanou;Kim, Yuri
    • Journal of Nutrition and Health
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    • v.49 no.2
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    • pp.111-124
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    • 2016
  • Purpose: The current study was conducted for evaluation and comparison of dietary behavior and food intake in different regions of Lao PDR. Methods: The survey was conducted on 979 people aged 40~59 years old living in 25 urban provinces and 25 rural provinces in four districts (VTE Capital-Chanthabuly, Xaysetha, VTE Province-Phonhong, and Thoulakhom) of Laos. General demographic information, health status, and dietary behavior were surveyed using a questionnaire. Results: The literacy ratio (p = 0.000), education level (p = 0.000), asset ownership level (p = 0.000), and government and private employee ratio (p = 0.000) were higher in urban subjects compared with rural subjects. The mean value of weight (p = 0.000), waist circumference (p = 0.000), and diastolic blood pressure (p = 0.009) and alcohol consumption (p = 0.000), self-rated health status (p = 0.001), and the rate of obesity (p = 0.000) were significantly higher in urban subjects compared with rural subjects. However, the rate of current smoker was significantly higher in the rural group (p = 0.023). Meals are becoming more westernized by higher frequency of eating out, consumption of fatty meat and fried or stir-fried food in urban areas compared to rural areas. Urban subjects had relatively better balanced meals compared to rural subjects whereas they consumed insufficient meals per day and consumed meals irregularly compared to rural subjects. Intake of fruit and milk was significantly higher in urban subjects compared with rural subjects. However, the intake of vegetables was significantly higher in rural areas than urban areas. Conclusion: The result of this study showed that the traditional Lao diet is being replaced by an unhealthy western dietary pattern, which may be a risk factor for increasing development of non-communicable disease (NCD) in Lao PDR. Planning of proper personalized nutritional intervention and education in each area is needed to decrease the health risks of NCD.

The Effect of Nutrition Education on the Improvement of Psychosocial Factors Related to Vegetable and Fruit Intake of Elementary School Children in Pre-Action Stages (채소와 과일섭취 증진을 위한 영양교육이 행동전단계 초등학생의 사회심리적 요인의 변화에 미치는 효과)

  • Suh, Yoon-Suk;Chung, Young-Jin
    • Journal of Nutrition and Health
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    • v.43 no.6
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    • pp.597-606
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    • 2010
  • This study investigates the change of level of psychosocial factors of decisional balance, processes of change, and self-efficacy after nutrition education on vegetable and fruit intake of children in pre-action stages. Forty-seven 5th grade elementary school children in Yeongi-Gun, Chungnam-Do were categorized into pre-action stages from a total of 71 children by four-stage questions, and then finally into two stages of precontemplation and contemplation & preparation. After 6 weeks nutrition education, recognition of both of pros and cons of vegetable intake were improved in the two groups of precontemplation and contemplation & preparation. In fruits intake, however, recognition of pros was not changed after nutrition education in either stage, but recognition of cons was improved only in the precontemplation stage. However, no difference in any psychosocial variables was found between precontemplation stage and contemplation & preparation stage. After the nutrition education, the score of consciousness raising of vegetable intake belonging to cognitive process of change increased in the subjects of the precontemplation stage, wheres the scores of dramatic relief, self-reevaluation, environment reevaluation and social liberation increased in the group of contemplation & preparation. In fruit intake, however, no change was found except in the environment reevaluation in the precontemplation group. The behavioral process of change of vegetable and fruit intake did not show any change in either group after nutrition education. Self-efficacy scores of vegetable intake of keeping the foods at hand, eating the recommended number of servings and making time to eat increased in the precontemplation group, while in the contemplation & preparation group, self efficacy scores of shopping for a variety of vegetables, instead of keeping the foods at hands, as well as the scores of the rest two items of eating the recommended number of servings and making time to eat increased after nutrition education. However, in case of fruits intake, no difference was shown in the precontemplation stage except an item of keeping the foods at hand. The results indicated that the stage of change was progressed in the pre-action stage after nutrition education and the scores of many psychosocial factors increased in both pre-action stages. It is suggested that the recognition of cons as well as the cognitive process of change and self-efficacy could be best used as indicators of the assessment of nutrition education program for vegetable intake.

Rootzone Profile, Trickle Irrigation System and Turfgrass Species for Roof Turf Garden (옥상녹화에 적합한 지반, 점적 관수 및 잔디 선정)

  • 이재필;한인송;주영규;윤원종;정종일;장진혁;김두환
    • Asian Journal of Turfgrass Science
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    • v.17 no.4
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    • pp.155-163
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    • 2003
  • This study was conducted to find out suitable rootzone profile, irrigation system, and turfgrass species for roof turf garden. Treatments of profile with soil amendment were Mixture I: Perlite(PL)60%+Vermiculite(VC)20%+Peatmoss(PM)20%, Mixture II: PL60%+VC 10%+PM20%+Sand(SD)10%, Mixture III: PL60%+VC20%+PM20% and Mixture IV: PL60%+VC10%+PM20%+SD10%+Styrofoam 5cm as a drain layer. To test trickle irrigation for roof garden, intervals of main pipe spacing(50cm, 100cm) and drop hole distance(15, 20, 30, 50 and 100cm)were treated, To select most suitable turfgrass species or mixture, Bermudagrass 'Konwoo', Zoysiagrass 'Konhee' and cool-season grass(Kentucky Bluegrass 80% + Perennial Ryegrass 20%, Tall Fescue 30% + KB50% + PR 20%)were tested. In particle size analysis, the soil amendments Perlite and vermiculite showed very even distribution, however, peatmoss contained mostly coarse particles with fiber over $\Phi$ 4.75mm. Under field moisture condition, vermiculite and peatmoss showed 350% water holding capacity, on the other hand, sand or Perlite showed 115% and 166%, respectively. Total weight of soil profile was 139.2kg/$m^2$ with Styrofoam drain layer without sand, which showed most lightest among treatments. Turf quality also resulted positve with Styrofoam drain layer installation. On trickle irrigation system, the proper interval of main drain pipe spacing and drop hole distance were 50cm and 50cm, respectively, In irrigation frequency, once per a day for 15 minute irrigation with 2 1/hr showed the best results on turf quality. Among turfgrass species or cool season grass mixture, warm season turfgrass fine leaf type zoysiagrass 'Konhee' and Bermudagrass 'Konwoo' showed very acceptable result on all over the treatments of rootzone and irrigation system. To apply cool season grasses for the roof garden, advanced researches may be needed to establish the proper soil amendment, rootzone profile, and irrigation system, Application of Bermudagrass 'Konwoo' for roof turf garden also needs successive tests to overcome winter injuries.

Effects of early commercial milk supplement on the mucosal morphology, bacterial community and bacterial metabolites in jejunum of the pre- and post-weaning piglets

  • Hu, Ping;Niu, Qingyan;Zhu, Yizhi;Shi, Chao;Wang, Jing;Zhu, Weiyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.3
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    • pp.480-489
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    • 2020
  • Objective: Sow milk (SM) may not be able to meet the piglet's nutritional needs in late lactation. Hence, this study was conducted to investigate the effects of early commercial milk (CM) supplement on the mucosal morphology, bacterial community and bacterial metabolites in jejunum of piglets. Methods: Ten litters of newborn piglets ([Yorkshire×Landrace]×Duroc) were randomly divided into 2 groups of 5 litters. The piglets in the control group were suckled by the sow (SM), while the piglets in the treatment group (CM supplement) were supplemented with a CM supplement along with suckling from d 4 to d 28 of age. Results: No significant differences were observed about jejunal mucosal morphology on d 28 and d 35 between two groups. On d 28, the activity of lactase in the jejunum was significantly decreased in the CM group, while the activity of sucrase and the ratio of maltase to lactase were significantly increased (p<0.05). On d 35, the activity of maltase in the jejunum was significantly increased in the CM group (p<0.05), and maltase to lactase ratio tended to increase in the CM group (p = 0.065). In addition, piglets in the CM group had a higher abundance of Clostridium XI, Tuicibater, and Moraxella in the jejunum on d 28, while the abundance of Lactobacillus was significantly increased on d 35 (p<0.05). Conclusion: The early CM supplement improved the maturation of the jejunum to some extent by enhancing the maltase and sucrase activities. Moreover, the early CM supplement could help maintain the homeostasis of internal environment in jejunum by increasing the microbial-derived metabolites.

Tea Consumption, Alcohol Drinking and Physical Activity Associations with Breast Cancer Risk among Chinese Females: a Systematic Review and Meta-analysis

  • Gao, Ying;Huang, Yu-Bei;Liu, Xue-Ou;Chen, Chuan;Dai, Hong-Ji;Song, Feng-Ju;Wang, Jing;Chen, Ke-Xin;Wang, Yao-Gang
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7543-7550
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    • 2013
  • Objective: To evaluate associations between tea consumption, alcohol drinking and physical activity and breast cancer risk among Chinese females. Methods: Three English databases (PubMed, ScienceDirect and Wiley) and three Chinese databases (CNKI, WanFang and VIP) were independently searched by 2 reviewers up to December 2012, complemented by manual searches. The quality of included studies was assessed with the Newcastle-Ottawa Scale items. Random-effects models were used to estimate the pooled odds ratios (ORs) and 95% confidence intervals (CIs). Potential publication bias was estimated through Egger's and Begg's tests. Heterogeneity between studies was evaluated with $I^2$ statistics. Results: Thirty-nine studies involving 13,204 breast cancer cases and 87,248 controls were identified. Compared with non-drinkers, regular tea drinkers had decreased risk (OR=0.79, 95%CIs: 0.65-0.95; $I^2$=84.9%; N=16). An inverse association was also found between regular physical activity and breast cancer risk (OR=0.73, 95%CIs: 0.63-0.85; $I^2$=77.3%; N=15). However, there was no significant association between alcohol drinking and breast cancer risk (OR=0.85, 95%CIs: 0.72-1.02; $I^2$=63.8%; N=26). Most of the results from the subgroup analysis were consistent with the main results. Conclusion: Tea consumption and physical activity are significantly associated with a decreased risk of breast cancer in Chinese females. However, alcohol drinking may not be associated with any elevation of risk.

Ultrastructure of nasal cilia in children with recurrent or persistent respiratory diseases (반복 지속성 호흡기 질환 소아에서 비강 섬모의 미세구조 검사의 의의)

  • Shin, Su-A;Yang, Seung;Oh, Jae-Won;Lee, Ha-Baik;Park, Cheol-Eon;Kwan, Jung-Kyun
    • Clinical and Experimental Pediatrics
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    • v.49 no.4
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    • pp.410-416
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    • 2006
  • Purpose : Ciliary abnormalities of the respiratory system usually accompany recurrent or persistent respiratory diseases such as paranasal sinusitis, bronchiectasis, rhinitis, and/or otitis media, since they cause certain derangements in ciliary cleaning activities. This disease is usually inherited by autosomal recessive trait, but may also be found to be acquired or transient in rare cases after heavy exposure to pollutants, cigarette smoking or severe infection. We performed this study in children with frequently recurrent or persistent respiratory diseases to clarify if the ciliary abnormalities are preceding factors. Methods : We enrolled 17 children with suspected respiratory ciliary abnormalities. The indications for evaluation of ciliary ultrastructure were recurrent or persistent respiratory infections. Children with immunologic abnormalities were excluded. From August 2000 to July 2003, we performed a biopsy on nasal mucosa and examined the structure of ciliary status by using an electron microscope. Results : Of the subjects, there were seven males and 10 females, aged 2 to 10 years. Out of the 17 subjects, 12 cases of chronic paranasal sinusitis, nine chronic coughs, nine frequent upper respiratory infections, seven cases of recurrent otitis media, four cases of recurrent pneumonia, and four cases of bronchial asthma were found. Out of the 17 cases on which histologic examinations were conducted, four cases showed pathologic findings, including one case of inner dynein arm defect, one of microtubular transposition, one of supernumerous tubules, and one singlet, respectively. Conclusion : It is essential for differential diagnosis and effective treatment to identify the abnormalities of ultrastructure of nasal cilia in children with symptoms of frequently recurrent or persistent respiratory diseases, if immunodeficiency or respiratory allergy could be excluded.