• Biomolecules & Therapeutics
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• v.30 no.6
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• pp.553-561
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• 2022

Chronic myeloid leukemia (CML) is a slowly progressing hematopoietic cell disorder. Sphingosine kinase 1 (SPHK1) plays established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers, including leukemia. However, small-molecule inhibitors targeting SPHK1 in CML still need to be developed. This study revealed the role of SPHK1 in CML and investigated the potential anti-leukemic activity of hirsuteine (HST), an indole alkaloid obtained from the oriental plant Uncaria rhynchophylla, in CML cells. These results suggest that SPHK1 is highly expressed in CML cells and that overexpression of SPHK1 represents poor clinical outcomes in CML patients. HST exposure led to G2/M phase arrest, cellular apoptosis, and downregulation of Cyclin B1 and CDC2 and cleavage of Caspase 3 and PARP in CML cells. HST shifted sphingolipid rheostat from sphingosine 1-phosphate (S1P) towards the ceramide coupled with a marked inhibition of SPHK1. Mechanistically, HST significantly blocked SPHK1/S1P/S1PR1 and BCR-ABL/PI3K/Akt pathways. In addition, HST can be docked with residues of SPHK1 and shifts the SPHK1 melting curve, indicating the potential protein-ligand interactions between SPHK1 and HST in both CML cells. SPHK1 overexpression impaired apoptosis and proliferation of CML cells induced by HST alone. These results suggest that HST, which may serve as a novel and specific SPHK1 inhibitor, exerts anti-leukemic activity by inhibiting the SPHK1/S1P/S1PR1 and BCR-ABL/PI3K/Akt pathways in CML cells, thus conferring HST as a promising anti-leukemic drug for CML therapy in the future.

• Animal Bioscience
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• v.35 no.12
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• pp.1921-1928
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• 2022

Objective: This research aimed to evaluate the effects of age on growth, tibia development, and intestinal calcium (Ca) and phosphorus (P) transporter gene expressions in broiler chickens. Methods: A total of 224 male Arbor Acres broilers were fed with nutrient-adequate diets and reared in eight cages (28 broilers per cage). Eight broilers (one broiler per cage) were selected and killed at 5, 10, 15, 20, 25, 30, 35, and 40 days of age, respectively. Results: Body weight continuously increased with age of broiler chickens from 5 to 40 days. The bone weight, ash weight, diameter, and length of the tibia also increased with broiler age. By contrast, the tibia ash, Ca, and P percentages quadratically changed with age (p<0.001), and the highest values of mineral contents were observed at 20, 25, and 25 days of age, respectively. The mRNA abundances of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), and plasma membrane ATPase 1b (PMCA1b) increased from 5 to 25 days and then decreased up to 40 days. Similar results were noted in the mRNA abundances of IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1), inorganic phosphate transporter 2 (PiT-2), nuclear vitamin D receptor (nVDR), and membrane vitamin D receptor (mVDR). The mRNA abundances of Ca and P transporters and VDRs were the highest at 25 days of age. Conclusion: These data indicate that age quadratically affects intestinal Ca and P transporter gene expression and mineral absorption capacity in broiler chickens.

• Journal of the East Asian Society of Dietary Life
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• v.20 no.5
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• pp.668-679
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• 2010

The purpose of this study was to analyze the relations of children's skipping meals after researching eating habits and lifestyle, parents' appreciation in nutrition behavior and dietary intake, throughout the research based on 4th to 6th grade students, total of 362 children at an elementary school in Inchoen. There were 104 students in skipping meals group and 258 students in eating meals group, with the average ages of 10.9, and 10.8, respectively. The average height and weight were 144.5 cm, 38.6 kg for skipping meals group, and 145.7 cm, 39.3 kg for eating meals group. Parents' appreciation of importance in breakfast showed a significant difference in whether children skip the meals or not (p<0.01). 43.7% of parents in skipping meals group answered that they serve breakfast everyday, compared to eating meals group with the percentage of 94.9%, showing significant difference in frequency of serving breakfast for their children (p<0.001). The skipping meals group answered that the reason they do not have breakfast is because they do not have time, which showed the highest percentage of 41.2%. For the eating meals group, 40.5% of students answered that they do not have appetite, which also showed difference (p<0.001). The skipping meals group tended to wake up later than those who have breakfast in the morning(p<0.01). The breakfast time for skipping meals group was later than the eating meals group, and according to whether they have breakfast of not, it showed a difference as well(p<0.01). Total score of nutrition attitude in skipping meals group and eating meals group were 30.8 and 32.1, showing that eating meals group showed more good in nutrition attitude (p<0.05). Daily intakes of energy (p<0.01) and protein (p<0.01) in skipping meals group were significantly lower than those in eating meals group. Skipping meals group bad lower rates in INQs of protein (p<0.01) and zinc (p<0.01), showing that skipping meals group is having low quality meals in nutrition. In conclusion, this study revealed that students with skipping meals are more likely to have meals that lacks nutrition or have low quality meals, and the time of rising hour in the morning, frequency of eating snacks can also affect whether or not they skip meals.

• The Korean Journal of Pesticide Science
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• v.5 no.3
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• pp.1-11
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• 2001

New technologies will have a large impact on the discovery of new herbicide site of action. Genomics, combinatorial chemistry, and bioinformatics help take advantage of serendipity through tile sequencing of huge numbers of genes or the synthesis of large numbers of chemical compounds. There are approximately $10^{30}\;to\;10^{50}$ possible molecules in molecular space of which only a fraction have been synthesized. Combining this potential with having access to 50,000 plant genes in the future elevates tile probability of discovering flew herbicidal site of actions. If 0.1, 1.0 or 10% of total genes in a typical plant are valid for herbicide target, a plant with 50,000 genes would provide about 50, 500, and 5,000 targets, respectively. However, only 11 herbicide targets have been identified and commercialized. The successful design of novel herbicides depends on careful consideration of a number of factors including target enzyme selections and validations, inhibitor designs, and the metabolic fates. Biochemical information can be used to identify enzymes which produce lethal phenotypes. The identification of a lethal target site is an important step to this approach. An examination of the characteristics of known targets provides of crucial insight as to the definition of a lethal target. Recently, antisense RNA suppression of an enzyme translation has been used to determine the genes required for toxicity and offers a strategy for identifying lethal target sites. After the identification of a lethal target, detailed knowledge such as the enzyme kinetics and the protein structure may be used to design potent inhibitors. Various types of inhibitors may be designed for a given enzyme. Strategies for the selection of new enzyme targets giving the desired physiological response upon partial inhibition include identification of chemical leads, lethal mutants and the use of antisense technology. Enzyme inhibitors having agrochemical utility can be categorized into six major groups: ground-state analogues, group specific reagents, affinity labels, suicide substrates, reaction intermediate analogues, and extraneous site inhibitors. In this review, examples of each category, and their advantages and disadvantages, will be discussed. The target identification and construction of a potent inhibitor, in itself, may not lead to develop an effective herbicide. The desired in vivo activity, uptake and translocation, and metabolism of the inhibitor should be studied in detail to assess the full potential of the target. Strategies for delivery of the compound to the target enzyme and avoidance of premature detoxification may include a proherbicidal approach, especially when inhibitors are highly charged or when selective detoxification or activation can be exploited. Utilization of differences in detoxification or activation between weeds and crops may lead to enhance selectivity. Without a full appreciation of each of these facets of herbicide design, the chances for success with the target or enzyme-driven approach are reduced.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.526-531
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• 2014

This study was carried out to compare the growth characteristics and feed values of the whole crop silage rice from paddy field and reclaimed paddy field. The heading dates of tested varieties was August 8 to August 30 in the paddy field, 1~2 days later than the reclaimed paddy field's August 6 to August 29. Plant height was in the range of 105~135 cm in paddy field, and 97~126 cm in reclaimed paddy field, respectively. The plant height of Mokyang was tallest in the paddy field, but Suwon 544 was tallest in the reclaimed paddy field. The number of tiller per plant of Nokyang was largest in the paddy field. Dry matter (DM) yield of whole crop rice ranged from 15.26 to $23.24MT\;ha^{-1}$ in the paddy field and 11.94 to $18.89MT\;ha^{-1}$ in the reclaimed paddy field. The highest DM yield in both fields was attained by Mokwoo. Comparing with the paddy field, the reclaimed paddy field achieved 78~84% in the DM yield. The crude protein content of varieties was 8.5 to 11.6% in the reclaimed tidal land and 6.7~8.7% in the paddy field. Neutral detergent fiber (NDF) of varieties in both fields ranged from 31.2 to 55.5%. Suwon 544 recorded the highest NDF contents in both fields. All varieties did not show any significant difference in acidic detergent fiber (ADF) which had values ranging from 22.3 to 33.2%. Total digestion nutrient (TDN) was more than 60% overall in both fields. Regarding TDN yield, Mokwoo recorded $16.54MT\;ha^{-1}$ in reclaimed tidal land and $12.69MT\;ha^{-1}$ in the paddy field, which showed the highest figure among the varieties. These results suggest the feasibility for cultivation of the whole crop silage rice in reclaimed paddy field, which showed 80% of the yield compared to paddy field, and implied that Mokwoo would show the most excellent yield and feed value.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.3
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• pp.335-341
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• 2013

Kyungokgos purchased in local markets in Korea vary in their combination and mixing ratios during processing. This study was investigated qualities of Kyungokgos manufactured traditionally to evaluating its qualities. The general components of Kyungokgos were moisture (18.62~49.78%), ash (0.198~1.211%), protein (0.89~3.58%), lipid (0.16~1.14%) and carbohydrates (47.95~77.08%). The color values of L, a, and b were 26.49~73.87, 16.51~38.64, and 45.41~88.94, respectively. The viscosity was classified into three non-Newtonian type groups: high, medium, and non-dilatant, according to the increase of loop execution times. Three extracts (KOG-1, -7, and -8, in a 30-fold dilution) showed no cytotoxicity toward RAW 264.7 cells, while the extracts of KOG-2, -4, and -5 showed a low cytotoxic effect. KOG-1 and -2 extracts with low cytotoxicity markedly inhibited the production of the inflammatory mediators-nitric oxide (NO) and tumor necrosis factor-alpha (TNF-${\alpha}$) in LPS-stimulated RAW 264.7 cells. These results indicate that KOG-1 and -2 extracts have anti-inflammatory activity in LPS-stimulated RAW 264.7 macrophages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.25-34
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• 2010

Although whole crop barley (Hordeum vulgare L.) is now widely grown as a silage crop in Korea, forage production and silage quality of that for organic farm have not been published. Therefore, this experiment was conducted to evaluate the effect of harvest stage on forage production and quality of organic barley, and effect of field wilting and crushed rice for shortening of harvest date and improvement of forage quality. The experiment was split-plot design with three replications. Main plots were heading, milking and yellow stages, and sub-plot were field wilting for I day, crushed rice 10% (CR10%) and 15% (CR15%) treatments. The dry matter (DM) contents of heading, milking and yellow stages were 12.8%, 21.9% and 29.8%, respectively. The DM yields of heading, milking and yellow stages were 10,346, 15,819 and 18,336 kg/ha, respectively, and the total digestible nutrients (TDN) of these were 6,288, 9,550 and 10,178 kg/ha, respectively. The pH of milking stage showed low 4.00 pH values. The crude protein, ether extract, crude ash, non-fiber carbohydrate (NFC) and TDN contents were decreased as harvest stage progressed, while neutral detergent fiber (NDF) and acid detergent fiber (ADF) were increased. The crude ash (CA) of milking stage showed the lowest among harvest stages. Field wilting and crushed rice treatments decreased CA, NDF and ADF contents, and increased NFC and TDN contents. In vitro dry matter digestibility (IVDMD) decreased with progressed harvest stage, while field wilting and crushed rice treatments increased that of barley silage. Lactic acid and total organic acid contents of milking stage were the highest, and butyric acid of milking stage was the lowest among harvest stage. The good effect of field wilting and crushed rice was observed in heading stage. The experiment results indicate that optimum harvest stage of barley silage for organic was milking stage. The field wilting and crushed rice additive could be recommended as effective method for shortening harvest date and increasing forage quality of organic barley silage

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.2
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• pp.115-120
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• 2010

This study was carried out to determine the forage production and quality of Italian ryegrass (IRG) and forage barley sown in early spring in Suwon, 2009. The five treatments used in this experiment were IRG Kowinearly (early maturity), IRG Kowinmaster (medium maturity), Yuyeon barley, Kowinearly + Yuyeon mixture, and Kowinmaster + Yuyeon mixture. The lodging was observed in IRG, but no lodging was found IRG + barley mixtures. The heading date of Kowinearly and Kowinmaster were 16 May and 22 May, respectively, and that of barley was 13 May. The dry matter (DM) percentage at harvest was 22.2~27.6%. The forage quality among treatments were similar, but the crude protein (CP) content of IRG was higher than that of barley, and in vitro DM digestibility was a little low in Kowinmaster. The yields of DM, CP and digestible DM were high in Kowinearly + Yuyeon barley mixtures as a 13,816 kg, 1,384 kg and 10,387 kg per ha, respectively (p<0.05). In conclusion, the mixture cultivation of IRG and forage barley was very effective, because of preventing of IRG lodging, increasing of forage yield, and stable production of forages. The optimum harvest date for silage manufacture of IRG and barley sown in early spring was recommended early June instead of May.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.2
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• pp.169-178
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• 2010

The present study was conducted with four ruminally fistulated cattle to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility and TDN values of various silages (whole crop rye, RS; whole crop barley, BS; Italian ryegrass, IRGS and sudangrass, SGS) which were produced in Korea. The pH of rumen fluid were not greatly affected by silage at most times after feeding but SGS decreased (p<0.049) the ruminal ammonia-N concentration as 7.28 mg/100 ml at 3h after feeding compared to other silages. An acetate proportion from SGS feeding was higher at 1h (p<0.018), 3h (p<0.004) and 6h (p<0.019) after feeding than those from other silages. The propionate ($C_2$) proportion, however, was greater (p<0.001~p<0.042) for the RS and BS than the corresponding values of other silages up to 6h after feeding while the lowest $C_2$ proportion was observed from SGS. The butyrate proportion was lowest (p<0.007~p<0.027) in the cattle fed BS at 1~6h after feeding among silages. An ED of dry matter (DM) in the rumen was highest (P<0.048) for RS as 59.64%, and was reduced in the order of BS (56.12%), IRGS (55.64%) and SGS (54.02%). Similar tendency was observed in the EDs of crude protein (p<0.014) and organic matter (OM, p<0.039). The whole tract digestibility of DM (p<0.032), neutral detergent fiber (NDF, p<0.034) and OM (p<0.041) of SGS was greatly reduced. The TDN value based on whole tract digestibility of silages was highest for RS as 61.1%, and TDN values of BS, IRGS and SGS were 57.1%, 57.9% and 50.7%, respectively. Based on the results obtained from the present study, components in various whole crop silages produced in Korea might affect the ruminal VFA proportion, and especially, NDF content could greatly influence on the TDN value of silage calculated based on the NDF content.

• Journal of Life Science
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• v.30 no.10
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• pp.877-885
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• 2020

Lentinuls edodes has been used for traditional food and medicine around Asia, and a variety of biological effects have been reported. In this study, L. edodes water extract (LWE) was investigated for its anti-photodamage effect in HaCaT keratinocytes. To perform the necessary assays, L. edodes was extracted with distilled water for 8 hr at 40℃ in an extract tank. Anti-photodamage activity was assessed using a scratch wound healing assay, cell proliferation, and a reactive oxygen species (ROS) scavenging test and by measuring the mRNA and protein expression levels of matrix metalloproteinases (MMPs) and type I procollagen. MMPs and collagen expression are major markers of UV-induced photodamage in skin. Prior to photodamage analysis, the total polyphenol and β-glucan contents of the LWE were evaluated and found to be 4.64 mg GAE/g DW and 165.96 mg/g, respectively. Treatment with LWE induced cell migration and cell proliferation in UV-irradiated HaCaT cells, and LWE effectively scavenged the ROS induced by H₂O₂ and UVB irradiation in HaCaT cells. UVB irradiation induced ROS generation and led to increased production of MMP-1 and MMP-9 and to decreased collagen production in human keratinocytes. Treatment with LWE upregulated the expression levels of MMP-1, MMP-9, and type I procollagen in UVB-irradiated HaCaT cells. This study suggests that LWE could be used to develop cosmetic materials with anti-photodamage effects.