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The Comparative Study of Arthropods Community on Non-transgenic Mother Chili Pepper (P915) and Transgenic Chili Pepper (CMVP0-CP) in the Isolated Quarantine LMO Fields (LMO 격리 포장에서 유전자비변형 모본 고추(P915)와 유전자 변형 고추(CMVP0-CP)에 서식하는 절지동물 군집 비교연구)

  • Yi, Hoon-Bok;Kim, Hyun-Jung
    • Korean journal of applied entomology
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    • v.49 no.1
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    • pp.23-29
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    • 2010
  • The environmental risks of cucumber mosaic virus resistant transgenic chili peppers with the CMVP0-CP gene on non-target organisms in the agroecosystem environments was evaluated during the periods of the chili pepper growing season (June 19, July 30, August 31) in 2007. Arthropods assemblages leaves and flowers of chili peppers were quantitatively collected by using an insect vacuum collector to compare the arthropod community structures between non-transgenic chili peppers (nTR, P 915) and mosaic virus resistant transgenic chili peppers (TR, CMV-cp, line 7). There was no statistical difference in the arthropod community structure between the two types of crops, nTR and TR, at the same season, although the species richness and Shannon's index were somewhat different among seasons; indicating no effects of genetically modified peppers on the arthropod community. However, further studies were required to conclude more concretely for the potential environmental risk of the transgenic chili pepper of CMV-cp.

Transformation Characteristics of Calcined Oyster Shell to Liquid Lime (소성된 굴패각의 액상소석회로의 전환 특성)

  • Ha, Su Hyeon;Kim, Yeongkyoo
    • Korean Journal of Mineralogy and Petrology
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    • v.33 no.3
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    • pp.185-193
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    • 2020
  • There have been many studies on the calcination of oyster shells in the perspective of recycling of resources. The quicklime made by the calcination of oyster shells is used either as it is or after reacting with water to transform to liquid lime before being used. However, the liquid lime made from calcined oyster shells show slightly different properties from that of limestone. In this study, to compare these properties of oyster shell with those of limestone, the samples were calcined and reacted with water at various temperatures to transform to a liquid lime and filtered using 150 ㎛ sieves to calculate the transform rate to liquid lime. The calcined limestone was transformed to liquid lime at all temperatures, but calcined oyster shell did not show any transformation at 30℃ and 50℃ under the experimental conditions of this study, and rather increased the weight for the remaining after filtration due to the presence of Ca(OH)2 produced by the reaction with water, Even at 90℃, the transformation rate of calcined oyster shell to liquid lime was lower than that of limestone. This difference in oyster shell can be explained partly by the preventing calcined one from reacting with water by conchiolin which is protein found in the prismatic and pearl layers of oyster shell. Conchiolin is also known to be stable and does not decompose even at high temperature. However, even the calcined chalk layer without conchiolin shows lower transformation rate than that of calcined limestone, probably due to the small amount of Na in oyster shell, which may cause additional reaction including eutectic melt during calcination process.

Investigation of Conservative Genes in 168 Archaebacterial Strains (168개 고세균 균주들의 보존적 유전자에 관한 연구)

  • Lee, Dong-Geun;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.30 no.9
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    • pp.813-818
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    • 2020
  • The archaeal clusters of orthologous genes (arCOG) algorithm, which identifies common genes among archaebacterial genomes, was used to identify conservative genes among 168 archaebacterial strains. The numbers of conserved orthologs were 14, 10, 9, and 8 arCOGs in 168, 167, 166, and 165 strains, respectively. Among 41 conserved arCOGs, 13 were related to function J (translation, ribosomal structure, and biogenesis), and 10 were related to function L (replication, recombination, and repair). Among the 14 conserved arCOGs in all 168 strains, 6 arCOGs of tRNA synthetase comprised the highest proportion. Of the remaining 8 arCOGs, 2 are involved in reactions with ribosomes, 2 for tRNA synthesis, 2 for DNA replication, and 2 for transcription. These results showed the importance of protein expression in archaea. For the classes or orders having 3 or more members, genomic analysis was performed by averaging the distance values of the conservative arCOGs. Classes Archaeoglobi and Thermoplasmata of the phylum Euryarchaeota showed the lowest and the highest average of distance value, respectively. This study can provides data necessary for basic scientific research and the development of antibacterial agents and tumor control.

Fatty Acid Composition and Functional Properties of Low Density Lipoprotein and Oxidized LDL from Human Plasma (인체 혈장에서 분리한 LDL과 LDL의 지방산 조성과 기능성의 변화)

  • Jae-Hoon Choi;Hyun-Mi Cho;Heung-Soo Son;Tae-Woong Kim
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.3
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    • pp.402-408
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    • 1994
  • Human plasma low density lipoprotein (LDL) is the major factor of coronary heart disease.But recent studies suggest the normal LDL can be realdily oxidized by oxygen free radicals and not interact with LDL receptors.Lipoprotei particles consist of lipid and protein, and fatty acids are prone to oxidatioin.The fatty acid compositions of LDL from Koreans was compared with that of Westerners.From the results, the raio of unsaturated fatty acids of korean and Westerner approximately 30 and 70%, respectively.which means Westerners are more labile in the lipid oxidation of LDL than Koreams.Normal LDL was incubated with $CuSO_4$ in PBS to lead for the peroxidation of LDL, and it was tested by the detection of TBARS and free radicals.Then, ascorbate, ${\alpha}-tocopherol$ and hyaluronic acid were found to have effects of antioxidants on LDL oxidation.The amount of free radical increased as the extent of oxidation increased.The time course of free radical formation was similar to TBARS.Therefore, determination of free radical by Luminometer was much more convenient than that of TBARS.

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Content Analysis of Food and Nutrition unit in Middle School Textbooks of Home Economics - Focus on the National Curriculums from 1st to 2009 revised (중학교 가정(기술·가정)교과 식생활 영역의 핵심 교육내용 분석 - 제1차 교육과정부터 2009개정 교육과정의 교과서 내용을 중심으로 -)

  • Jang, Yoon-Mi;Kim, Yoo Kyeong
    • Journal of Korean Home Economics Education Association
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    • v.30 no.4
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    • pp.93-112
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    • 2018
  • We analysed the textbooks of Home Economics in middle school from 1st to 2009 curriculums to investigate the contents and the portion of Food and Nutrition section. The key words were generated by word cloud technique using text-mining, and the portion of Food and Nutrition section was presented as a ratio of the pages. The core key words of Food and Nutrition section through the curriculums were 'raw food'·'food'·'diet'. In 1st and 2nd curriculums, the main key words were related to food materials, condiments and nutrients such as 'vitamin'·'protein'. The words such as 'nutrition'·'eating'·'requirement' were newly appeared in 3rd, 'portion' in 6th, and 'diet'·'adolescence' in 7th curriculum. The mean ratio of Food and Nutrition section in Home Economics was 24.3%. While the portion was as high as 31.8% in 7th it was strikingly reduced to 15.2% in 2009th. curriculum. Besides, Food and Nutrition section was composed of 10 units of middle level category during the 2nd and 3rd curriculums, and was reduced to 2 small units with none of middle level category in 2009th curriculum. Although the contents of Food and Nutrition section has been developed and adapted to the needs of the society through the curriculums, the portion of Food and Nutrition section in Home Economics has been reduced especially in 2009th curriculum, which could raise concerns on the health of individuals and communities.

Effects of Prunellae Herba on the H2O2-Treated LLC-PK Cell's Redox Status and NF-κB Signaling (H2O2 처리된 LLC-PK₁세포에서 Redox Status 및 NF-κB Signaling에 대한 하고초(夏枯草)의 효과)

  • Son, Jong Seok;Jeong, Ji Cheon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.30 no.4
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    • pp.242-249
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    • 2016
  • This study was to investigate the anti-inflammatory effects of Prunellae Herba(PH). The generation of superoxide anion radical (․O2-), nitric oxide (NO), peroxynitrite (ONOO-) and Prostaglandin E₂(PGE2) were measured in the H2O2-Treated renal epithelial cells(LLC-PK1 cell) of mouse. And the effects of Prunellae Spica on the expression of NF-κB (p50, p65), IKK-α, phospho-IκB-α and inflammation-related proteins, COX-2, iNOS, IL-1β and VCAM-1, were examined by western blot. The fluorescent probes, 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihyldrorhodamine 123 (DHR 123) were used to estimate the scavenging effect of Prunellae Spica on ․O2-, NO, ONOO-. Western blot was conducted to assess the protein expression levels of NF-κB (p50, p65), IKK-α, phospho-IκB-α, inflammation-related proteins, COX-2, iNOS, IL-1β, VCAM-1. PH inhibited H2O2-treated cell death dose-dependently. It reduced the generation of ·O2-, NO, ONOO- and PGE₂ in the H2O2-treated renal epitheial cells(LLC-PK1 cell) of mouse in vitro. PH reduced the expression of NF-κB, IKK-α, phospho-IκB-α, COX-2, iNOS, IL-1β and VCAM-1 genes through means of decreasing activation of NF-κB signaling as well. According to these results, PH has an antioxidative activity and anti-inflammatory effect by regulating the NF-κB pathway. This suggest that PH is expected to be used to regulating inflammatory process and treating inflammation-related disease.

Changes in Chemical Composition of Traditional Ewhaju druing Brewing (전통 이화주 양조 중의 화학성분 변화)

  • 김정옥;남상명;김종군
    • Korean journal of food and cookery science
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    • v.9 no.4
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    • pp.272-277
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    • 1993
  • Chemical composition was determined to evaluate the quality of traditional Ewhaju during brewing. The content of moisture, total sugar, reducing sugar, crude protein and crude ash of Ewhaju after the lapse of 20 days from brewing were 47.01%, 47.57%, 29.45%, 4.81% and 0.09%, respectively and free sugar composed with 28.07% of glucose and 1.30% of maltose compared with 17.43% of glucose in one year aged Ewhaju. Contents of minerals were Ca 4.8mg%, Mg 9.2mg%, K 33.0mg%, Na 16.0mg%, Mn 0.76 ppm, Fe 35.0 ppm, Zn 1.1 ppm, Cu 0.12 ppm, Cr 0.20 ppm and Pb 0.22 ppm and rarely changed during fermentation. Total amino acids were 4.23% immediately after brewing 4.54% after the lapse of 100 days. In change of amino acids, methionine and tyrosine slightly increased during fermentation. Total acid contents of Ewhaju at immediately after brewing, in the lapse of 100 days and aged one year were 0.25%, 2.16%, 3.70%, respectively and 0.05%, 0.72% and 1.04%, in volatile acid. The pH in Swhaju remarkably decreased during fermentation; 6.1 at immediatly after brewing, about 3.9 at 100 days and 365 days fermentation.

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Inheritance and Heritability of Telomere Length in Chicken (닭 텔로미어 길이의 유전력 추정과 유전 전이 양상)

  • Park, Dan Bi;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.41 no.3
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    • pp.217-225
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    • 2014
  • Telomeres are the ends of the eukaryotic chromosomes and consist of a tandem repetitive DNA sequence and shelterin protein complex. The function of telomere is to protect chromosome. Telomere length in somatic cells tends to decrease with organismal age due to the end replication problem. However, several factors at the genetic, epigenetic and environmental level affect telomere length. In this study, we estimated heritability of telomere length and investigated inheritance of telomeres in a chicken. Telomere length of lymphocytes was analyzed by semi-quantitative polymerase chain reaction using telomere primer and quantitative fluorescence in situ hybridization using telomeric DNA probe. In results, heritability of telomere length was estimated 0.9 at birth by offspring-parent regression analysis and was estimated 0.03 and 0.04 at 10 and 30 weeks old, respectively, by parental variance analysis. There was a significant positive correlation in telomere length between father and their offspring (r=0.348), and mother and their offspring (r=0.380). In inheritance patterns of telomere length, the influence of paternal and maternal effect on their offspring was similar. The influence of inherited telomeres on male and female progeny was also roughly alike. These results implicated that imprinting of parental telomere length was regulated by autosomal genes, not sex linked genes. In addition, telomere length of offspring at birth did not differ along with their maternal age. Thus, maternal age does not affects telomere length in their offspring at birth owing to cellular reprogramming at early embryonic stage.

Effects of Shading and Organic Matter Applications on Growth and Aromatic Constituents of Codonopsis lanceolata (차광과 유기물 시용이 더덕의 생육 및 향기성분에 미치는 영향)

  • 이승필;김상국;남명숙;최부술;이상철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.496-504
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    • 1996
  • The study was conducted to obtain the basic information on increasing aromatic degrees by shading and organic matter application on Condonopsis lanceolata. The result were as followings : Fresh root wt. in shading 55 % treatment was increased about twices, whereas fresh root weight in non-shading was decreased. But growth of ground parts such as vine length, leaf width, and leaf number was promoted under non-shading treatment. Macroelements such as K, Ca, and Mg, were increased in non-shading and higher organic matter application. The components such as crude protein, fiber, and ash were increased in the shading 55% treatment compared with non-shading. In the sixteen amino acids, arginine contents were the highest in non-shading and organic matter 30M/T. Recovery yield of essential oil was 0.005% in non-shading. The highest was organic matter 30M/T in the non-shading as 0.007%. Major aromatic constituents were 11 kinds of aliphatic alcohols such as I-hexanol, cis-3-hexenol, and trans-2-hexanal. As a result, major aromatic degree was higher in shading 55%, also increased in the much organic matter application. It was considered to be applied dense shading 55% and much organic matters to produce Condonopsis lanceolata plants which have high aroma and good quality.

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The effect of duck meat treated with turmeric powder intake on the postprandial blood lipid profiles in female university students (울금 첨가 오리가공육 섭취가 여대생의 식후 혈중 지질 농도에 미치는 영향)

  • Lee, Songmi;Ro, Hee Kyong
    • Journal of Nutrition and Health
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    • v.49 no.2
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    • pp.80-87
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    • 2016
  • Purpose: This study examined the effects of duck meats with turmeric powder on blood lipids in 10 female university students. Methods: The subjects received duck meat with 0%, 0.1%, 0.2%, and 0.4% turmeric powder and glucose, total cholesterol, triglyceride (TG), high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol in their serums after 30, 60, 90, 120, and 180 min were measured. Results: The average height, weight, and body mass index of subjects were $159.6{\pm}2.6cm$, $51.3{\pm}3.5kg$, and $20.1{\pm}1.0$, respectively. The fasting glucose, ${\gamma}-glutamyl$ transferase (GGT), glutamic pyruvic transferase (GPT), glutamic oxaloacetic transferase (GOT), c-reactive protein (CRP), and hemoglobin were within the normal range. The ${\Delta}-AUC$ (area under the curve) of postprandial glucose, TG did not change, but ${\Delta}-AUC$ of postprandial total cholesterol and LDL were significantly decreased, and HDL was increased by intake of the duck meat with turmeric powder. Conclusion: This study shows that duck meats with turmeric powder affected the postprandial blood lipid levels.