• KSBB Journal
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• v.14 no.3
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• pp.309-314
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• 1999

In order to produce high concentration of sodium gluconate, optimization of the fermentation conditions, such as glucose concentration, inoculum size, dissolved oxygen concentration and glucose feeding method, was examined. When the glucose concentration was maintained in the range of 30∼50 g/L during the batch fermentation, glucose conversion yield and productivity were 92.2% and 6.0 g/L/hr, respectively. In the case of the low concentration below 30 g/L, the yield decreased by about 25%. As the inoculum size increased above 20%(w/v), lag phase was shortened but the productivity decreased. The dissolved oxygen level of 60∼70% was shown to be the threshold point for 75% of increase in the productivity of sodium gluconate. Finally, optimal glucose feeding rate was determined using various feeding methods such as exponential feeding, feeding based on the average glucose consumption rate and was determined using various feeding methods such as exponential feeding, feeding based on the average glucose consumption rate and on the oxygen uptake rate and etc. Our result shows that glucose feeding, based on the oxygen uptake rate is a very simple, efficient and robust method, especially when oxygen is consumed as a substrate for the bioconversion. Using the above glucose feeding strategy under the optimized condition, 255 g/L of sodium gluconate concentration, 12 g/L/hr of productivity and 95% of glucose conversion yield were achieved with A. niger ACM53.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.23 no.2
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• pp.83-87
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• 2017

This study was conducted to determine types of oxygen transmission rate (OTR) films suitable for modified atmosphere (MA) storage treatment of strawberries (cv. 'Maehyang') for export and examination related changes in quality of strawberries during simulated shipping distribution conditions ($2^{\circ}C$ for $10days{\sim}8^{\circ}C$ for 11 days). Strawberries were packed by 1,300, 10,000, 20,000, and $30,000cc/m^2{\cdot}day{\cdot}atm$ OTR films for MA storage treatment and perforated film as the control for MAP. Fresh weight loss rate was less than 0.5% in OTR films except for the perforated film. Carbon dioxide and oxygen content in packages prevailed in permissible range for strawberries under recommended CA/MA conditions (i.e., $CO_2$: 15~20%, $O_2$: 5~10%) at 10,000 cc, 20,000 cc, and 30,000 cc OTR films. Ethylene content in OTR films did not reveal significant differences during storage. The 1,300 cc OTR film revealed highest off-flavor and lowest fungal incidence rate by sensory evaluation. The firmness, soluble solids, and visual quality were preserved the highest at 10,000 cc OTR film. In conclusion, 10,000 cc OTR film preserved the highest quality and extended shelf-life by 13 days if compared with conventional distribution conditions.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.502-502
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• 2023

지난 140여년(1880~2022)간 지구의 연평균 기온은 약 1.5℃ 상승하였다. 이에 따라 폭염, 홍수, 가뭄 등 이상기후 발생이 급증하고 있으며, 생태계가 급격히 변화하여 담수호의 산소량 또한 급속히 감소하고 있다. 소양호는 총길이 60 km, 유역면적 2.703 km², 최대저수량 29억톤으로, 유역면적은 북한강 유역의 25%에 달하며 북한강 상류에 위치하고 있는 국내 최대의 인공호이다. 따라서 소양호 유역의 관리는 북한강 유역의 수질 관리와 수도권 수자원 공급의 핵심 중 하나라고 볼 수 있다. 그러나 기후변화에 따라 소양호의 성층현상에도 변동이 생겼을 것으로 판단되고 있다. 호소의 성층현상은 수심에 따른 온도의 변화로 발생하는 현상으로, 성층현상이 심하면 유체는 연직운동이 제한되고 상대적으로 수평방향 운동이 활발해진다. 소양호는 수심이 매우 깊으며, 열용량이 크기 때문에 여름에 성층이 형성되는 온대일순환호(warm monomictic lake)로 분리된다. 성층현상이 심하면 호소 하부의 저층에서는 용존산소가 거의 없어 혐기성 상태가 되고, 침전된 유기물이 혐기성 미생물에 의해 분해되기 때문에 수질은 크게 악화된다. 따라서 본 연구는 30년간의 소양호의 수온과 DO의 변동을 분석하여 성층현상의 변동성을 파악하고자 하였다. 이에 따라 약 30년간(1993년 1월~2022년 12월) 0~90 m까지 측정한 수온, DO 데이터를 이용하였다. 데이터는 매달 최소 1회~최대 5회 측정된 자료 중 가장 수심이 깊게 측정된 날의 자료를 이용하였다. 1월과 2월의 데이터는 동절기로 인해 소양호 조사를 실시할 수 없어 제외하였다. 수온의 경우 30년간 서서히 증가하는 경향을 보였으며 0~90m 전반에 걸쳐서도 대체로 증가하는 경향을 보였다. 또한 6월에 성층현상이 더 심해지고, 겨울에는 연직운동이 감소하는 모습을 보였다. DO의 경우 용존산소가 중층에서 최저를 보이는 Metalimnetic oxygen minimum을 보였으며, 2008년까지는 거의 매년 농도 2.0mg/L 이하인 달이 있었으나 그 이후에는 회복되어 대부분 2.0 mg/L 이상을 보였다. 그러나 심층의 경우에는 2014년부터 DO 농도 3 미만으로 떨어지는 경우가 증가하였다.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.28 no.1
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• pp.41-50
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• 2023

Dissolved oxygen sensors have characteristics in which data drift occurs over time. Therefore, in-situ calibration of the dissolved oxygen sensor is essential to accurately measure the concentration of dissolved oxygen in seawater. In order to provide a method for in-situ calibration, appropriate number of samples for calibration, and laboratory calibration interval of the dissolved oxygen sensor, the dissolved oxygen sensor values were compared with the measured values by titration on a total of 133 samples from three different cruises in the Indian Ocean, Pacific Ocean, and East Sea over a period of about one year. As a result, it is preferable to calibrate the sensor value using the correlation of a straight line obtained by directly comparing the final concentration value given by the sensor and the measured value. For the accurate calibration, at least 30 samples must be used to enable in-situ calibration within an accuracy range of about 1%. In addition, it is recommended that a laboratory calibration should perform within 1 year for the membrane type dissolved oxygen sensor for CTD to achieve a performance of 70% or more.

• Food Science and Preservation
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• v.5 no.3
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• pp.207-210
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• 1998

To prolong the shelf life of green chili pepper, packaging films such as 30 $\mu\textrm{m}$ low density polyethylene(LDPE), 20 $\mu\textrm{m}$ perforated polyolefin (SM250), 30 $\mu\textrm{m}$ cast polypropylene (CPP) and 15 $\mu\textrm{m}$ polyolefin (MPD) were used. LDPE showed a suitable gas concentration which consisted of 3-5% O$_2$ and below 5% CO$_2$for keeping quality of green chili peppers after 10 days. Though weight loss was 9.3% for SM250 after 5 days, others showed below 2.0% weight loss after 40 days. Green chili peppers inside CPP package revealed a great decrease in ascorbic acid content after 30 days. SM250 showed a less total microbial count among packages, but there was no great differences among packages.

• Applied Biological Chemistry
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• v.39 no.3
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• pp.172-176
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• 1996

Effect of culture conditions such as pH, temperature, agitation speed and oxygen transfer rate on xylitol production from xylose by Candide parapsilosis ATCC 21019 mutant was investigated in a jar fermentor. The initial concentration of xylosr was fixed at 50 g/l in this experiment. When pH was increased, cell growth and xylose consumption rate were increased, but maximum xylitol production was shown in the range of pH 4.5 and 5.5 with a yield of 0.68 g/g-xylose. The optimal temperature for xylitol production was determined to be $30^{\circ}C$. Considering the importance of dissolved oxygen tension, for xylitol production, the effect of oxygen transfer rate coefficient $(k_La)$ on fermentation parameters was carefully evaluated in the range of $20{\sim}85\;hr{-1}\;of\;k_La$ (corresponding to $100{\sim}300$rpm of agitation speed). The xylitol production was maximized at $30\;hr^{-1}\;of\;k_La$(150 rpm). A higher oxygen transfer rate supported better cell growth with lower xylitol yield. It was determined that maximum xylitol concentration, xylitol yield and productivity was 35.8 g/l, 71.6% and $0.58\;g/l{\sim}hr^{-1}$, respectively, at $30\;hr^{-1}\;of\;k_La$ In order to further increase xylitol productivity, ferementation using the concentrated biomass(20 g/l) was carried out at the conditions of pH 4.5, $30^{\circ}C$ and $30\;hr\;1$ of oxygen transfer rate. The final xylitol concentration of 40 g/l was obtained at 18 hours of culture time. From this result, it was calculated that xylitol yield was 80ft on the basis of xylose consumption and volumetric productivity was $2.22\;g/l{\sim}hr$ which was increased by $3{\sim}4$ fold compared with $0.5{\sim}0.7\;g/l-hr$ obtained in a normal fermentation condition.

• Food Science of Animal Resources
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• v.32 no.3
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• pp.346-353
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• 2012

This study was carried out in order to investigate the relationship of antioxidant enzyme activity, lipid oxidation, and aroma pattern of Hanwoo (Korean cattle) beef for 8 d at $15^{\circ}C/RH$ 100% under 0% $O_2$/20% $CO_2/80%\;N_2$, 25% $O_2$/20% $CO_2/55%\;N_2$, 50% $O_2$/20% $CO_2/30%\;N_2$, and 75% $O_2$/20% $CO_2/5%\;N_2$-conditions. The reduction of $O_2$ and the induction of $CO_2$ were shown in the 25-75% $O_2$-groups during storage and total change rates of $O_2$ and $CO_2$ were followed in order: 25%>50%>75%. Catalase activity was not significantly different among all groups. During storage, glutathione peroxidase activity decreased by increasing the $O_2$ concentration and was followed at 8 d in order: 0%>25%>50%>75% (p<0.05). From 2 d of storage, TBARS content had significant (p<0.05) differences in order: 0%>25%>50%>75%. The CIE $L^*$ value was higher (p<0.05) in the 25-75% $O_2$-groups, compared with the 0% $O_2$-group. During the first time, the CIE $a^*$ value was higher (p<0.05) in order: 0%<25%<50%<75% but was lower (p<0.05) in the 25-75% $O_2$-groups from 2 d of storage than in the 0% $O_2$-group. The CIE $b^*$ value was higher (p<0.05) in the 25-75% $O_2$-groups than in the 0% $O_2$-group and had significant (p<0.05) differences at 0 and 8 d in order: 0%<25%<50%<75%. Aroma patterns with the electronic nose were clearly discriminated between the 0% $O_2$-groups and the 25-75% $O_2$-groups at 8 d of storage. Therefore, high oxygen reduced stabilities of antioxidant enzymes, lipid oxidation, and color in Hanwoo beef. Furthermore, antioxidant enzyme activities and lipid oxidation could affect aroma patterns.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.5
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• pp.874-881
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• 1997

In order to estimate toxicity of copper, radmium and chromium on survival, growth and oxygen consumption of the estuarine mysid, Neomysis awatschensis, adult and juvenile, the experiments were conducted by renewal bioassay method at $20{\pm}1^{\circ}C\;and\;20{\pm}1\%_{\circ}$ salinity. The $96hr-LC_{50}$ of the mysid exposed to cadmium, copper and chromium was 20.2, 11.3 and $670.4\;{\mu}g/\ell$ in adut and 3.4, 1.9 and $49.4\;{\mu}g/\ell$ in juvenile, respectively, and were ranked in order of toxicity : copper >cadmium >chromium. Survival rates of the mysid exposed to the sublethal concentrations of heavy metals for 40 days were significantly affected by cadmium $\geq1.0\;{\mu}g/\ell$ and copper $\geq0.6\;{\mu}g/\ell$ concentrations. The growth rate of the mysid exposed to $cadmium\geq2.0\;{\mu}g\ell$ and copper $\geq1.2\;{\mu}g/\ell$ concentrations were significantly reduced than that exposed to normal condition, but there was no affect on intermoult period. Oxygen consumption rate of the mysid exposed to heavy metals was significantly reduced with increasing heavy metals concentrations. The results of the present study led us to conclude that concentrations levels cadmium$\geq1.0\;{\mu}g\ell$ and copper $\geq0.6\;{\mu}g\ell$ of the estuarine could markedly affect the distribution and population of the mysid by reduced survival, growth and oxygen consumption rate.

• Tuberculosis and Respiratory Diseases
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• v.63 no.3
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• pp.242-250
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• 2007

Background: Abnormal angiogenesis can induce hypoxia within a highly proliferating tumor mass, and these hypoxic conditions can in turn create clinical problems, such as resistance to chemotherapy. However, the mechanism by which hypoxia induces these changes has not yet been determined. Therefore, this study was conducted to determine how hypoxia induces changes in cell viability and extracellular microenvironments in an in vitro culture system using non-small cell lung cancer cells. Methods: The non-small cell lung cancer cell line, A549 was cultured in DMEM or RPMI-1640 media that contained fetal bovine serum. A decrease in the oxygen tension of the media that contained the culture was then induced in a hypoxia microchamber using a $CO_2-N_2$ gas mixture. A gas analysis and an MTT assay were then conducted. Results: (1) The decrease in oxygen tension was checked the anaerobic gas mixture for 30 min and then reoxygenation was induced by adding a 5% $CO_2-room$ air gas mixture to the chamber. (2) Purging with the anaerobic gas mixture was found to decrease the further oxygen tension of cell culture media. (3) The low oxygen tension resulted in a low pH, lactic acidosis and a decreased glucose concentration in the media. (4) The decrease in glucose concentration that was observed as a result of hypoxia was markedly different when different types of media were evaluated. (5) The decrease in oxygen tension inhibited proliferation of A549 cells. Conclusion: These data suggests that tumor hypoxia is associated with acidosis and hypoglycemia, which have been implicated in the development of resistance to chemotherapy and radiotherapy.

• Journal of Chest Surgery
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• v.34 no.7
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• pp.524-533
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• 2001

Background: Hyperoxemic cardiopulmonary bypass (CPB) has been recognized as a safe technique and is widely used in cardiac surgery. However, hyperoxemic CPB may produce higher toxic oxygen species and cause more severe oxidative stress and ischemia/reperfusion injury than normoxemic CPB. This study was undertaken to compare inflammatory responses and myocardial injury between normoxemic and hyperoxemic CPB and to examine the beneficial effect of normoxemic CPB. Material and method: Thirty adult patients scheduled for elective cardiac surgery were randomly divided into normoxic group (n=15), who received normoxemic CPB (about Pa $O_{2}$ 120 mmHg), and hyperoxic group (n=15), who received hyperoxemic CPB (about Pa $O_{2}$ 400 mmHg). Myeloperoxidase (MPO), malondialdehyde (MDA), adenosine monophosphate (AMP), and troponin-T (TnT) concentrations in coronary sinus blood were determined at pre- and post-CPB. Total leukocyte and neutrophil counts in arterial blood were measured at the before, during, and after CPB. Lactate concentration in mixed venous blood was analyzed during CPB, and cardiac index (Cl) and pulmonary vascular