Applied Biological Chemistry
- Volume 39 Issue 3
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- Pages.172-176
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- 1996
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Optimization of Culture Conditions for Xylitol Production by A Mutant of Candida parapsilosis
Candida parapsilosis 돌연변이주에 의한 Xylitol 생산조건의 최적화
- Oh, Deok-Kun (Department of Food Science and Technology, Woosuk University) ;
- Kim, Sang-Yong (Tong Yang Confectionery Co., R&D Center) ;
- Kim, Jung-Hoe (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
- Published : 1996.09.30
Abstract
Effect of culture conditions such as pH, temperature, agitation speed and oxygen transfer rate on xylitol production from xylose by Candide parapsilosis ATCC 21019 mutant was investigated in a jar fermentor. The initial concentration of xylosr was fixed at 50 g/l in this experiment. When pH was increased, cell growth and xylose consumption rate were increased, but maximum xylitol production was shown in the range of pH 4.5 and 5.5 with a yield of 0.68 g/g-xylose. The optimal temperature for xylitol production was determined to be
Candida parapsilosis ATCC 22019 돌연변이주를 이용하여 xylitol 생산에 영향을 주는 배양 조건인 pH와 온도 그리고 교반속도 및 산소전달속도등의 환경인자가 Xylitol의 생성에 미치는 영향을 살펴보았다. 발효조에서 pH가 증가 할수록 균체농도와 기질소비속도가 증가하여 발효시간이 단축되었다. 그러나, Xylitol생산은 pH 4.5와 5.5에서 큰 차이 없이 50g/l의 xylose로 부터 약 34g/l로 최대농도를 보여주었다. 온도가 증가 할수록 최대 비증식속도가 증가하였지만 최종 균체농도는 감소하였고, xylitol 생산성은