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Efficacy and Safety Evaluation of an Air Sterilizer Equipped With an Electrolytic Salt Catalyst for the Removal of Indoor Microbial Pollutants (염촉매 전기분해 공기살균기의 효능 평가)

  • Sun Nyoung Yu;Ho-Yeon Jeon;Bu Kyung Kim;Ae-Li Kim;Kyung Il Jung;Gye Rok Jeon;Soon Cheol Ahn
    • Journal of Life Science
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    • v.34 no.7
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    • pp.500-508
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    • 2024
  • Recently, there has been increasing interest in enhancing the indoor air quality, particularly in response to the growing utilization of public facilities. The focus of this study was on assessing the efficacy and safety of an air sterilizer equipped with electrolytic salt catalysts. To that end, we evaluated the antimicrobial activity of the vapor spraying from the air sterilizer and its cytotoxicity in condensed form on human cell lines (HaCaT, BEAS-2B, and THP-1). Against the test organisms, which comprised five bacterial strains (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium) and one fungal strain (Candida albicans), the air sterilizer exhibited relatively high antimicrobial activities ranging from 10.89 to 73.98% following 1 and 3 hr of vapor spraying, which were notably time-dependent. Importantly, cytotoxicity assessments on human cells indicated no significant harmful effect even at a 1.0% concentration. Comprehensive safety evaluations included morphological observations, gene expression (Bcl-2, Bax) tests, and FACS analysis of intracellular ROS levels. Consistent with previous cytotoxicity findings, these estimates demonstrated no significant changes, highlighting the air sterilizer's safety and antimicrobial activities. In a simulated 20-hr operation within an indoor environment, the air sterilizer not only showed an 89.4% removal of total bacteria but also a 100.0% removal of Escherichia sp. and fungi. This research outlines the potential of the developed electrolytic salt catalyst air sterilizer to effectively remove indoor microbial pollutants without compromising human safety, underscoring the solution that it offers for improving indoor air quality.

Seasonal Variations of Water Quality in the Lower Part of the Nagdong River (낙동강 하류수질의 계절적 변화)

  • KIM Yong-Gwan;SHIM Hye-Kung;CHO Hak-Rae;YOU Sun-Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.6
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    • pp.511-522
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    • 1984
  • The Nagdong is one of the biggest rivers in Korea, which is very important water source not only for tap water of Pusan city but also for the industrial water. Therefore, authors tried to check the water quality year by year. In this experiment one hundred and twenty water samples collected from August 1983 to July 1984 were analyzed bacteriologically and physiologically. Fifteen sampling stations were established between near Samrangjin and estuary of the river. To evaluate the water quality, temperature, pH, chloride ion, salinity, chemical oxygen demand (COD), electrical conductivity, nutrients, total coliform, fecal coliform, fecal streptococcus, viable cell count and bacterial flora were observed. The variation of water temperature was ranged $-1.5{\sim}29.0^{\circ}C$ (Mean value $13.9{\sim}16.5^{\circ}C$), it in spring was higher as $10{\sim}15^{\circ}C$ about $10^{\circ}C$ than in winter and it in autumm was very stabilized as about $20^{\circ}C$ at each station. The pH variation of the samples was ranged $6.68{\sim}9.15$. The range of concentration of chloride ion and salinity varied $7.4{\sim}l,020.5$ mg/l and $1.05{\sim}33.0\%0$, respectively. Especially, salinity of the 3rd water war was the higher than others as $25.76{\sim}31.58\%0$. COD was ranged $1.45{\sim}14.94$ mg/l and the lower part of the Nagdong River was heavily contaminated by domesitc sewage and waste water from the adjacent factor area. The range of electrical conductivity was $1.360{\times}10^2{\sim}5.650{\times}10^4{\mu}{\mho}/cm$ and that was by far higher the estuary than the upper. Concentration of nutrients were $0.008{\sim}0.040$ mg/l (Mean value $0.019{\sim}0.068$ mg/l) for $NO_2-N,\;0.038{\sim}5.253$ mg/l ($0.351{\sim}2.347$ mg/l) for $NO_3-N,\;0.100{\sim}2.685$ mg/l($0.117{\sim}1.380$ mg/l) for $NH_4-N,\;0.003{\sim}0.084$ mg/l($0.014{\sim}0.065$ mg/l) for $PO_4-P$ and $0.154{\sim}6.123$ mg/l ($1.165{\sim}3.972$ mg/l) for $SiO_2-Si$, respectively. Usually nutrients contents of the water in the upper part(included station 1 to 5) were higher than those of the estuarine area. The bacterial density of the samples ranged 7.3 to 460,000/100 ml for total coliforms, 3.6 to 460,000/100 ml for fecal coliform, $0{\sim}46,000/100ml$ for fecal streptococcus and $<30{\sim}1.2{\times}10^5/ml$ for viable cell count. Composition of coliform was $28\%$ Escherichia coli group, $18\%$ Citrobacter freundii group, $31\%$ Enterobacter aerogenes group and $22\%$ others. Predominant species among the 659 strains isolated from the samples were Pseudomonas spp. ($42\%$), Flavobacterium spp. ($20\%$) and Moraxella spp. ($12\%$).

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Effect of Nitrous Oxide (N2O) Treatment on Quality of Peach (Prunus persica) Postharvest (복숭아(Prunus persica) 수확 후 아산화질소(N2O) 처리가 품질에 미치는 영향)

  • Nah, Hyun-Seok;Bae, Ro-Na;Lee, Seung-Koo
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.42-49
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    • 2012
  • This experiment was conducted to find out the effects of nitrous oxide ($N_2O$) on the postharvest quality of 'Janghowon hwangdo' peach fruits. Fruits were harvested at commercial maturity for marketing in late September, and treated with 70% $N_2O$ + 20% $O_2$ + 10% air, 80% $N_2O$ + 20% $O_2$, and 90% $N_2O$ + 10% $O_2$ for 48 h, and then stored at $15^{\circ}C$. No significant treatments for soluble sugar and titratable acidity contents were detected. However, good appearance and taste in peach fruit were maintained better in 80% $N_2O$ treatment than in air treatment. The treatment with 90% $N_2O$ had negative effects on weight loss and taste because of rotting by anaerobic fermentation. 80% $N_2O$ treated fruit had significantly higher fungus (Botrytis cinerea) growth inhibition of saprogenic approximately than air treatment until 12 days of storage. The browning and rotting at surface of peach were also retarded when peaches were treated with 80% $N_2O$ before they were artificially wounded. The activity of polyphenol oxidase (PPO) was inhibited about 80% in peach of 80% $N_2O$ treatment compared with in air treatment. The result showed that 80% $N_2O$ treatment was able to extend the shelf life of peach fruits through maintaining taste and inhibition of softening and browning by rotting and wounding during storage.

Effects of weir on the change of sediments (하천의 보가 퇴적물에 미치는 영향)

  • Cho, Yong-Chul;Kim, Kyung-Hee;Woo, Hyo-Seop;Oh, Jong-Min
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.1083-1087
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    • 2006
  • 과거 50 년 동안 국내 보 설치 수는 매년 증가하여 왔으나, 그 증가비율은 근래 들어 감소하는 추세로, 이는 보 보다는 용수공급능력이 큰 양수장, 저수지 등 타 시설물에 의존하는 것으로 판단 할 수 있다. 더욱이 인구의 도시 집중화에 따른 농경지의 도시화, 경작 방식의 변화에 의한 비닐하우스 단지로의 변화, 시설의 노후화 등으로 매년 $50{\sim}150$개 정도의 보가 폐기되고 있다. 그러나 이렇게 폐기되는 보는 하천에 그대로 존치되어 하천생태통로의 단절, 수질악화, 수변 서식처 악화 등의 문제를 지속적으로 야기 시키고 있다. 실험은 문산천 검전보, 안양천 안양대교보, 기철거된 경안천 무명보에서 자연시료를 채취하여 실행 하였다. 문산천 검전보는 하천환경이 안정되고 양호한 대조구간으로 선정하였다. 안양 대교보는 도심화로 인하여 원기능을 상실하였으며 수차례에 걸친 개수가 이루어졌고 수년전까지만 해도 러브보로 인한 수질정화장치가 있었으나 효능이 약화되어 철거할 계획을 가지고 있는 것으로 알려져 있다. 경안천 무명보는 현재는 완전 철거된 상태이다. 경안천 무명보는 기 철거된 보의 대조구간으 로 선정하였다. 보 상류의 수질 오염도가 높은 것은 정체구역에 의한 용존산소 부족과 오염저니에 의한 것으로 판단되므로, 각각의 대상보의 퇴적물 오염도를 조사하였다. 각각의 조사 지점은 검전보의 경우, 만장교를 최상류 지점으로, 검전보 상/하류, 광탄교를 최하류 지점으로 선정하여, 총 4 지점에서 채취했으며, 안양대교보의 경우 최상류지점, 보 상/하류 지점으로 총 3지점, 무명보의 경우 보 상/하류로 총 2 지점에서 채취했으며, 실험은 $\ulcorner$Soil Method$\lrcorner$에 준하여, 각각 입도분포, 함수율, 강열감량, COD, T-N, T-P 등을 시행하였다. 문산천 검전보 퇴적물 오염도 조사 결과, 강열감량, COD, T-N, T-P 항목에서 보 상류가 보 하류에 비해 높게 나타났으며, 각각 하류에 비해 상류가 30.76%, 16.69%, 73.13%, 18.91% 더 높게 나타났다. 안양대교보 퇴적물 조사 결과, 검전보와 마찬가지로, 강열감량, COD, T-N, T-P 항목에서 보 상류가 보 하류에 비해 33.33%, 9.23%, 24.78%, 18.56% 높게 나타났다. 기 철거된 무명보의 경우, 무명보 상류, 무명보 하류 2개 지점을 채취하였으며, 강열감량, COD, T-N 항목에서 보 상류가 보 하류에 비해 18.75%, 412.6%, 25.75% 높게 나타났다. 퇴적물 조사 결과, 강열감량, COD, T-N 항목에서 검전보, 안양대교보, 무명보 모두 보 상류의 오염도가 높게 나타났으며, 무명보를 제외한 검전보, 안양대교보는 T-P 항목에서도 보 상류의 오염도가 높게 나타나, 보 상류의 퇴적물 오염농도가 심화된 것으로 나타났다. 그러나 기철거된 무명보의 경우, 강열감량, COD, T-N, 항목에서 보 상류가 높게 나타났으나, 철거되지 않은 검전보나 안양대교보에 비해 그 차이가 크지 않은 것으로 나타났다.

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The Effect of Addition of Cyclic Adenosine Monophosphate and Nitric Oxide in Low Potassium Dextran Solution for Lung Preservation in an Isolated Rabbit Lung Perfusion Model. (토끼 폐장 분리관류 모형에서 Low Potassium Dextran 용액에 Cyclic Adenosine Monophosphate와 Nitric Oxide의 첨가가 폐보존에 미치는 영향)

  • 조덕곤;조규도;김영두;곽문섭
    • Journal of Chest Surgery
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    • v.34 no.3
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    • pp.212-223
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    • 2001
  • 배경: 이식폐의 보존 및 재관류 동안 cyclic adenosine monophosphate(cAMP)와 nitric oxide(NO)는 폐혈관 내 순환조절을 유지하는데 있어 중심적인 역할을 한다. 그러나 내치세포내의 cAMP와 NO 모두 허혈-재관류 과정 동안에 현저하게 감소한다. 이에 저자는 low potassium dextran(LPD) 폐조본액에 cAMP의 유사체인 dibutyry1 cAMP(db-cAMP)와 NO의 공여물질인 nitroglycerin(NTG)을 첨가하여 이들의 폐보존 효과를 알아보고, 이들은 첨가한 폐보존액 들의 효과를 비교하였다. 대상 및 방법: 토끼 폐장 분리관류 모형에 실험군은 각각 6마리씩 4개군으로 단순 LPD 페보존액만 사용한 경우(I군), LPD 용액에 NTG 만 참가한 경우(II군), cAMP 만 첨가한 겨우(III군) 그리고 두가지 모두를 첨가한 경우는 IV군으로 분류하였으며, 폐보존액이 주입된 심폐블록은 영상 1$0^{\circ}C$에서 24시간 동안 보관한 다음 100% 산소농도에서 기계호흡을 하면서 신선 정맥혈로 30분 동안 재관류를 시행하였다. 재관류폐의 평가를 위해 폐기능 및 폐부종 정도를 정량 측정하였으며, 유출로 혈액으로부터 tumor necrosis factor $\alpha$(TNF-$\alpha$)와 간접적인 NO의 총량을 알기 위해 nitrite/nitrate의 양을 측정하였다. 또한 재관류가 끝난 후 광학 및 전자현미경학적 소견을 관찰하였다. 결과: 모든 실험군 중 제 IV군 의 폐보존 능력이 가장 우수하였으나, 제 II, III, IV군 사이는 통RP적으로 유의한 차이가 없었다. 제 I군은 제 II, III, IV군들에 비해 유의하게 폐기능이 가장 나쁘고 폐부종 정도가 가장 심했다(p<0.05). 제 II군은 제 III군에 비해 더 좋은 폐기능을 보였고, 폐부종 정도가 덜 하였으나 통계적은 유의성은 없었다. TNF-$\alpha$ 는 제 IV 군이 Irns에 비해 유의하게 분비량이 적었다. (p<0.05). 총 NO의 양은 제 II군과 IV 군이 제 I 군과 III군보다 유의하게 높았으나(p<0.001), 제 II군과 IV군, 제 I군과 III군 사이 비교에서 유의한 차이는 없었다. 또한 제 I 군과 III군에서는 시간이 지남에 따라 유의하게 NO의 양이 점차 감소하였다. (p<0.05). 광학 및 전자현민경 소견상 폐포 및 폐혈관 구조가 제 II, III, IV 군이 I 군에 비해 더 잘 보존되어있었다. 결론: LPD 폐보존액에 db-cAMP 및 NTG의 첨가는 폐보존 효과가 모두 우수함을 확인하였고 이들의 폐보존 효과 차이는 거의 없음을 알수 있었다. 그렇지만 이들의 병합사용이 폐혈관 항상성을 더 잘 유지시킬 수 있고 허혈-재관류 손상을 줄여 폐보존 효과를 높일 수 있을 것이라고 기대된다.

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Evaluation of Dispersant Application to Stranded Oil as a Clean-up Technique at Sandy Tidal Flat (사질 조간대 표착유의 방제를 위한 유화분산제의 적용 평가)

  • Cheong, Cheong-Jo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.11 no.4
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    • pp.227-231
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    • 2008
  • The purpose of this study is to clarify the behavior of stranded crude oil and to estimate the restoration of seawater infiltration by application of dispersant as one of cleaning techniques. We made visualization of infiltration process of seawater and stranded crude oil on the sandy beach sediments by using of a model sandy beach. Major conclusions derived from this study are as follows. The seawater infiltration volume was reduced by the stranded crude oil. However, thirty percentage of the sweater infiltration into the sediments was restored by dispersant application to the penetrated oil in sandy beach. The penetration depth of stranded oil were dropped at first falling tide, but were not significantly fluctuated after that. Moreover, oil concentration was most high within the upper 2 cm. The stranded crude oil was broken into small size droplets and dispersed into the sediments by the dispersant application. Therefore, dispersant applications play an important roles in the large increase of surface area of given volume of oil, and it resulted in promoting to biological degradation process at the oil/water interface, dispersing the stranded oil into the water column and restoration of the supplement of the dissolved oxygen and nutrients to the benthic organisms.

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KINETIC STUDIES OF LACTIC ACID FERMENTATION(PART 2) INFLUENCE OF TEMPERATURE ON FERMENTATION (유산균 발효에 관한 동력학적 연구(제2보) 발효에 미치는 온도의 영향)

  • LEE Keun-Tai;LEE Myeong-Sook;HAN Bong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.3
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    • pp.161-166
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    • 1979
  • To know the influence of temperature on the fermentation process, a strain of Lactobacillus bulgarius was experimentally cultured three different temperature conditions of $39^{\circ}C,\;42^{\circ}C\;and\;45^{\circ}C$, pH 5.8 and mechanical agitation of 500rpm. During 20 hour's fermentation, the microbial growth attained the maximum concentration under the conditions mentioned above. However, the culturing conditions resulted different outcomes in terms of maximum concentration of the microbes and the residual concentration of substrate. Among the three temperature conditions, the fermentation at $45^{\circ}C$ was most effective and the maximum specific growth temperature conditions, the fermentation at $45^{\circ}C$ was most effective and the maximum specific growth rate was 0.58/hr. Activation energy deduced from the Arrhenius equation was 9,220cal/mole and entropy was $-33.74\;cal/^{\circ}K$ mole. Activation enthalpy was 9,845 cal/mole and free energy was 19,800 cal/mole.

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Changes of Soil Salinity due to Flooding in Newly Reclaimed Saline Soil (신간척지 토양에서 담수에 의한 토양염도 변화에 대한 개관)

  • Ryu, J.H.;Yang, C.H.;Kim, T.K.;Lee, S.B.;Kim, S.;Baek, N.H.;Choi, W.Y.;Kim, S.J.;Chung, D.Y.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.45-46
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    • 2009
  • This study was carried out to identify the changes of EC during desalinization due to flooding in newly reclaimed saline soil. To do this, experimental plots were made of rotary tillage+water exchanging plot, flooding plot and rainfall flooding plot. In rotary tillage+water exchanging plot, drainage, rotary tillage and flooding were conducted at the interval of 7 days. In rotary tillage+water exchanging plot and flooding plot, plots were irrigated at the height of 10 cm. After 38 days desalinization, changes of EC values at top soil (0~20 cm) were as follows. In rotary tillage+water exchanging plot, EC decreased from $21.38dS\;m^{-1}$ to $2.16dS\;m^{-1}$ and in flooding plot, EC decreased from $13.97dS\;m^{-1}$ to $2.22dS\;m^{-1}$. In rotary tillage+water exchanging plot and flooding plot, EC values decreased below the EC criterion ($4.0dS\;m^{-1}$) of saline soil. In rainfall flooding plot, EC values decreased or increased according to amounts of rainfall and rainfall time. After 38 days, EC decreased from $16.7dS\;m^{-1}$ to $12.35dS\;m^{-1}$. In flooding plot, changes of EC due to soil depth were investigated. After 38 days desalinization, changes of EC due to soil depth were as follows. At 0~10 cm depth, EC value decreased from $13.08dS\;m^{-1}$ to $0.74dS\;m^{-1}$ (94.3% of salt was desalinized). At 10~20 cm depth, EC value decreased from $14.80dS\;m^{-1}$ to $3.69dS\;m^{-1}$ (75.2% of salt was desalinized). At 20~30 cm depth, soil was desalinized slowly compared with upper soil, EC value decreased from $13.57dS\;m^{-1}$ to $6.93dS\;m^{-1}$ (48.9% of salt was desalinized).

Survival and Physiological Responses of Red Sea Bream Pagrus major with Decreasing Sea Water Temperature (수온 하강에 따른 참돔, Pagrus major의 생존율 및 생리 반응)

  • Shin, Yun Kyung;Kim, Young Dae;Kim, Won Jin
    • Korean Journal of Ichthyology
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    • v.30 no.3
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    • pp.131-136
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    • 2018
  • Decrease in seawater temperature during winter is one of the most important and serious issues confronted by fish net-cage aquaculture farms. This can become the cause of the manifestation of diseases and ensuing mass mortality. The present study was conducted to assess the survival rate, the range of limited low-temperature, the response of oxidative stress in the blood of red sea bream Pagrus major with decrease of water temperature. Low-lethal temperature for 7 days of P. major ($7day-LT_{50}$) was $6.54^{\circ}C$ (confidence limit: $6.31{\sim}6.71^{\circ}C$). Oxygen consumption rate was decreased with lower temperature. It showed the minimum value at the range of low-lethal temperature. Osmolality at $5^{\circ}C$ and $6^{\circ}C$ experimental group was higher significantly than control group. SOD (superoxide dismutase) activity was increased significantly at $5^{\circ}C$ experimental group compared to control group. This study data will be used to determine the appropriate area for aquaculture of red sea bream. It also manage fish net-cage farm to cope with the mass mortality occurring frequently during winter season.

Effects of Crack Resistance Properties of Ozone-treated Carbon Fibers-reinforced Nylon-6 Matrix Composites (탄소섬유의 오존처리가 나일론6 기지 복합재료의 크랙저항에 미치는 영향)

  • Han, Woong;Choi, Woong-Ki;An, Kay-Hyeok;Kim, Hong-Gun;Kang, Shin-Jae;Kim, Byung-Joo
    • Applied Chemistry for Engineering
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    • v.24 no.4
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    • pp.363-369
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    • 2013
  • In this work, the effects of ozone treatments on mechanical interfacial properties of carbon fibers-reinforced nylon-6 matrix composites were investigated. The surface properties of ozone treated carbon fibers were studied by Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). Mechanical interfacial properties of the composites were investigated using critical stress intensity factor ($K_{IC}$). The cross-section morphologies of ozone-treated carbon fiber/nylon-6 composites were observed by scanning electron microscope (SEM). As a result, $K_{IC}$ of the ozone-treated carbon fibers-reinforced composites showed higher values than those of as-received carbon fibers-reinforced composites due the enhanced $O_{1s}/C_{1s}$ ratio of the carbon fiber by the ozone treatments. This result concludes that the mechanical interfacial properties of nylon-6 matrix composites can be controlled by suitable ozone treatments on the carbon fibers.