• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.131-135
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• 1995

In winter, most of the energy for a greenhouse crop is supplied during the night. Since watermelon is grown under high night temperature, the experiments were set up to investigate night temperature influence on watermelon in order to obtain the best economic output. Day temperatures ranged from $25^{\circ}C$ and 3$0^{\circ}C$ ; night temperatures ranged from 2$0^{\circ}C$ to 3$0^{\circ}C$ at 5$^{\circ}C$ interval. Two cultivars of watermelon(Citrullus vulgaris S. ‘Binna’ and ‘Kamro’) treated with 30/3$0^{\circ}C$ yielded maximum leaf areas, flowers and leaf numbers. 30(14h)/25(10h)$^{\circ}C$ or 30(12h)/25(12h)$^{\circ}C$ grown plants had higher germination ratio and more dry weight and chlorophyll than those of 30/3$0^{\circ}C$ which were the highest temperature integral. Although 25/$25^{\circ}C$ and 30/2$0^{\circ}C$ regime are same average temperature, the growth of watermelons at 30/2$0^{\circ}C$ was significantly higher than 25/$25^{\circ}C$. ‘Binna’ was growing more than ‘Kamro’ at the same temperature. Leaf area ratio(LAR) was reduced with increasing DIF temperature from 30/3$0^{\circ}C$ to 30/2$0^{\circ}C$, but leaf weight ratio(LWR) was increased.

• Journal of fish pathology
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• v.25 no.1
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• pp.39-46
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• 2012

Experiments were carried out to determine the hematological changes in freshwater tilapia Oreochromis niloticus, after water temperature (20, 25 and $30^{\circ}C$) and arsenic concentrations (200, and 400 ppb) exposure 20 days. The RBC count and hematocrit (Ht) were significantly increased in As 400 ppb and water temperature $30^{\circ}C$. Hemoglobin (Hb) was significantly increased under combined As (200 and 400 ppb) and water temperature $30^{\circ}C$. The tilapia exposed to water temperature $30^{\circ}C$ and As concentration 400 ppb increase significantly in calcium concentration. The high concentrations of total protein and glucose concentrations were observed at water temperature $30^{\circ}C$ and As concentration 400 ppb. A significant increment GOT and GPT activities in the tilapia serum noticed at water temperature (25 and $30^{\circ}C$) and As concentration (200, 400 ppb). These results indicate that tilapia can be affected by high water temperature ($30^{\circ}C$) and As concentration (400 ppb) in terms of inorganic matter, organic matter and enzyme activity in serum.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.1-5
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• 1998

This study was investigated to find out the optimum rhizome size of ginger characteristics and yield at two planting densities. Seventy five gram of rhizome gave best performance for plant height and yield with comparison with the other sizes at two planting densities. Among 25g, 50g and 75g of ginger rhizome size were not significantly different in plant height and yield at $30{\times}30cm$ planting density. But, 25g of rhizome size is economically recommended at $30{\times}30cm$ planting densities.

• Korean Journal of Acupuncture
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• v.27 no.2
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• pp.95-105
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• 2010

Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on interleukin(IL) production in mouse macrophage stimulatedby lipopolysaccaride(LPS). Methods : Productions of interleukins were measured y High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$(multi-analyte profiling beads) technology. To begin with, cell culture supernatant was obtained after treatment with LPS(1 ${\mu}g/mL$) and SB for 24 hour. Then, it was incubated with the antibody-conj${\mu}g$ated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. After incubating for 30 minutes, Strepavidin-conjugated Phycoerythrin(SAPE) was then added. Incubating for another 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Results : The results of the experiment are as follows. SB significantly inhibited the LPS-induced production of IL-3($9.15{\pm}0.35$ pg/mL) by $6.92{\pm}0.05,\;7.21{\pm}0.11,\;6.96{\pm}0.33,\;and\;7.45{\pm}0.74$ pg/mL at the concentration of 25, 50, 100, and 200 ${\mu}g/mL$ in mouse macrophage RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced production of IL-5($7.30{\pm}0.48$ pg/mL) by $6.50{\pm}0.29,\;6.30{\pm}0.25,\;6.30{\pm}0.25,\;and\;5.80{\pm}0.25$ pg/mL at the concentration of 25, 50 100, and 200 ${\mg}g/mL$ in RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced productiion of IL-9($17.26{\pm}0.19$ pg/mL) by $15.01{\pm}0.43$ pg/mL at the concentration of 25 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced productioh of IL-13($187.80{\pm}2.90$ pg/mL) by $152.80{\pm}4.25,\;172.80{\pm}3.97,\;162.10{\pm}6.67,\;and\;165.30{\pm}11.80$ pg/mL at the concentration fo 25, 50, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-17($18.30{\pm}0.95$ pg/mL) by $13.30{\pm}1.25,\;13.80{\pm}1.11,\;13.30{\pm}0.75,\;and\;14.00{\pm}1.08$ pg/mL at the concentration of 25, 50 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-23($43.90{\pm}0.83$ pg/mL by $39.50{\pm}1.26,\;38.00{\pm}1.78,\;and\;39.60{\pm}2.49$ pg/mL at the concentration of 25, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). Conclusions : These results suggest that SB has anti-inflammatory activity related with its inhibition of IL-3, IL-5, IL-13, IL-17, and IL-23 production in macrophages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.362-366
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• 2022

This study was conducted to find out the changes in the growth and yield of ginseng and the changes in the soil of direct-seeded ginseng fields after applying different compaction strengths. As a result of surface soil compactions, the topsoil hardness increases as the strength of treatment increases in the first year but topsoil hardness increased only by applying 30 kg weight of compaction in the second year. The germination rate was significantly higher (79.4% and 79.1% at 25 kg and 30 kg, respectively) in 1st year after the application of soil surface compactions. The longest plant was 35.7 cm in 4- years old ginseng in the control and the height was 26.9 cm and 26.5 cm in the soil surface compactions of 25 kg and 30 kg, respectively. In addition, the higher weight of ginseng roots of 31.3 g and 30.3 g were observed after applying 25 kg and 30 kg compaction treatment, and the lowest root weight of 25.6 g was in the control. Therefore, it is shown that after sowing, applying the weight of 25 kg to 30 kg for soil surface compaction is appropriate for better yield in direct seeded cultivation of ginseng at paddy fields.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.755-757
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• 1999

This study was conducted to investigate the optimum temperature after BA treatments for the indution of flowering in Cymbidium ensifolium 'Tekkotsusosin'. Flowering rate was 90% at $30/25^{\circ}C$ (day/night), 50% at $25/20^{\circ}C$ and the natural condition as control, and non flowering at $20/15^{\circ}C$. Malformation rate of flower shape was at $25/20^{\circ}C$, 4% at $30/25^{\circ}C$, and 9% in the natural condition. The length and number of flowering stalks per plant were most at $30/25^{\circ}C$ treatment. After BA spraying, the lowest air temperature for flowering was assumed to be about $20^{\circ}C$.

• The Korean Journal of Malacology
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• v.31 no.1
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• pp.35-41
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• 2015

In order to investigate the live food value of microalgae for efficacious rearing of larvae and spats of bivalve, we studied growth rates of four microalgal species (Isochrysis galbana, Pavlova lutheri, Chaetoceros simplex, Tetraselmis tetrathele) cultured in different environmental conditions. These include changes in temperatures (20, 25, 30 and $35^{\circ}C$), salinities (20, 25, 30 and 33 psu) and light intensities (60, 100 and $140{\mu}mol\;m^{-2}s^{-1}$). The growth rate of I. galbana was faster at $25^{\circ}C$ than that of $20^{\circ}C$. At $25^{\circ}C$ the highest growth rate of I. galbana was observed at 33 psu (0.413) and the lowest at 20 psu (0.368) in 10 days of culture (P < 0.05). The growth rate of I. galbana was lower at 25 psu (0.383) than that of 30 psu and higher than that of 20 psu (P < 0.05). Similar temperature and salinity-dependent changes were also found in P. lutheri and T. tetrathele. C. simplex showed faster growth rate at $30^{\circ}C$ than that of $25^{\circ}C$. The highest growth rate of C. simplex was observed at 33 psu (0.428) and the lowest at 20 psu (0.389) in 10 days of culture (P < 0.05). Upon exposure to the light with different intensities, all four microalgal species showed a significantly faster growth rate at $140{\mu}mol\;m^{-2}s^{-1}$ than at $100{\mu}mol\;m^{-2}s^{-1}$ (P < 0.05).

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.4
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• pp.379-384
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• 1995

The retinomotor responses of jack mackerel, Trachurus japonicus and carp, Cyprinus carpio were studied for the different strobe flash frequencies in respects of the application of the light stimulus to herding and barrier system. The strobe flash frequencies ranged at 1.7, 5, 10, 25, 430Hz in jack mackerel and 1.7, 5, 25Hz in carp. The retinal adaptation ratio was examined histologically according to the time elasped of 1, 5, 10, 30min after turning on the light in each experimental condition. Cone index were determined in order to express the retinal adaptation ratio. Jack mackerel showed an increase of the retinal adaptation ratio ranging from average 27% at the pre-stimulus light to 42.6%, 49.8%, 59.8% and 83.1% at the irradiation times of 1, 5, 10 and 30min., respectively. Carp showed an increase of the retinal adaptation ratio ranging from average 28.4% at the pre-stimulus light to 51%, 53.9% and 91.3% at the irradiation times of 1, 5 and 30min., respectively. The light adaptation process was observed to be fast approximately in order of 430Hz, 25Hz, 5Hz, 1.7Hz and 10Hz for jack mackerel, and 25Hz, 5Hz and 1.7Hz for carp, and to be light-adaptated in 30 minutes.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.343-348
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• 2012

Developmental period, mummification, pupal mortality, and sex ratio of Korean strain of Aphelinus varipes F$\ddot{o}$rster parasitizing the green peach aphid, Myzus persicae were studied at 20, 25, 30, and $35^{\circ}C$ in controlled climate cabinets. The plastic container with a leaf disc of sweet pepper (Capsicum annuum L.) was used as experimental units. Green peach aphids at different developmental stages were reared in four different temperatures and presented to A. varipes for 12 hours. A female adult of A. varipes was allowed to parasitize for 12 hours in each plastic petri dish. The periods from egg to adult of A. varipes were 18.3 days, 14.7 days, 10.4 days, and 9.3 days at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The pupal mortalities of A. varipes were 0%, 1.5%, 2.8%, and 10.2% at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The mummifications of A. varipes were significantly different in all developmental stages of the aphid. The mummification of A. varipes parasitizing green peach aphid was the highest at 1st and 2nd in $20^{\circ}C$ and $25^{\circ}C$, but the highest at 3rd (84.4-85.0%) in $30^{\circ}C$ and $35^{\circ}C$. The host feedings of A. varipes were $20^{\circ}C$ (12.5%), $25^{\circ}C$ (17.3%), $30^{\circ}C$ (16%), and $35^{\circ}C$ (10.9%), respectively. The sex ratios of A. varipes female were $20^{\circ}C$ (49%), $25^{\circ}C$ (46%), $30^{\circ}C$ (69%), and $35^{\circ}C$ (60%), respectively.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.43-47
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• 1997

This study was carried out in the laboratory to clarify effects of different temperature of Protopulvinaria mangiferae(Green) on development, survivorship and reproduction. Developmental period of the mango shieldscale from crawlers to preoviposition adult decreased as temperature increased. The threshold temperature andthermal constants for the development of one generation were 11.7"C and 1000.0 day-degrees, respectively. At25$^{\circ}$C and 30$^{\circ}$C, survival rate from egg to preoviposition adult were 82% and 6096, respectively. Hatchability ofeggs was more than 99% at both condition. The reproductive period overaged 50 days(2SnC) and 33 days(30$^{\circ}$C). After mature adult began to reporduce, and more than 50% of the crawlers emerged during the firsthalf of their life time. The net reproduction rate per generation(R), mean length of a generation0 and intrinsicrate of natural increase(r, ) were higher at 25$^{\circ}$C than at 30"C, and the values measured at 25$^{\circ}$C were 132.6, 76.2 and 0.064/female/day, respectively.