• Journal of Aquaculture
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• v.7 no.4
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• pp.189-205
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• 1994

Formerly, adult-tiger puffer, Takifugu rubripes with ova caught in the sea, were used for seedling production. But it was difficult to secure naturally-ripened adults. For the purpose of adult tiger puffer in captivity, this study was carried out. To determine the growth 220 tiger puffers hatched in 1990 (3-year-old) and 1991 (2-year-old) were used. For spawning and egg incubation leading to fry development, eggs were stripped from tiger puffers hatched in 1988 (5-year-old) and 1990 (3-year-old) through human chorionic gonadotropin (BCG) treatments. In May, 1993, mean body length and mean body weight of 2-year-old tiger puffer were $30.72\pm1.35cm\;and\;1,048\pm228 g,$ and that of 3-year-old tiger puffers were $36.02\pm1.17cm$ and $1,402\pm66g$ respectively. The relationship between body length (L) and body weight (W) of 2-year-old the tiger puffers during the experiment period was represented as $W\;=\;1.7892L^{31524}\times10^5$ (r= 0.9436) and that of 3-year-old, $W=\;3.2840L^{36099}\times10^6$ (r= 0.9070) respectively. The GSI in female 2-year-old-fish changed from $0.23\times0.l2\;to\;0.74\pm0.08$, during the experiment period, and in male it didn't change remarkably until November, but thereafter it increased and showed a peak of $8.69\pm5.09$. The GSI of 3-year-old-fish showed a peak of $8.05\pm5.58$ in April in female and $12.65\pm4.60$ in May in male. The change of HSI in 3-year-old-fish was correlative to the change of GSI, but in 2-year-old-fish it was little correlative. In female gonad of 2-year-old tiger puffer, the mature oocytes reached $350{\mu}m$ in April, but thereafter they didn't spawn and became atrophied. But in male gonad, a great number of spermatozoa were crowded in the testicular lobuli in April. Female gonad of 3-year-old tiger puffer had the mature oocytes of 650 pm in March and the ripe oocytes, $900{\mu}m$ in April. Male testis development was similar to that of 2-year-old-fish. Egg-stripping after hormone treatments was possible past 139 hours and 142 hours from each of two 5-year-old-fish (500IU/kg, BW), and after 114 hour from a 3-year-old-fish (1,000 IU/kg, BW) under water temperature $16.3\~17.8^{\circ}C$. Eggs stripped amounted was 650 g and 400 g from two 5-year-old-fish and 610 g from the 3-year-old-fish, and fertilization rates were $98.0\%,\;97.4\%\;and\;96.5\%$ respectively. All the hatched larvae devloped into normal fry.

• Journal of Families and Better Life
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• v.28 no.1
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• pp.37-49
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• 2010

This study investigated the effects of three factors - children's age, sex and parental feeding styles - on children's self-help skills. The subjects were 213 children and their parents who lived in Kwanak-ku, Seoul. The three-way analysis of variance was employed. The main results were as follows: 1. Children's autonomy was varied significantly according to their sex. Girls do better by themselves than boys do in toileting, washing and drying hands, putting on and buttoning up a coat, putting on clothes, washing and drying their faces. Children's autonomy also varied significantly according to age. A majority of the autonomy subsets increased as the children's ages increased. However, drying their hands with a towel, removing their coats, and drinking water did not vary by age. 2. Children's autonomy also varied significantly according to their parents' feeding style. A responsive feeding style encouraged children to drink water by themselves. 3. The group with the highest score in self-help skills in toileting was 6-year-old girls, and the group with the lowest score was 3-year-old boys. Additionally, the groups with the highest scores in toileting were 4-year-old girls whose parents demonstrated a neglective feeding style, 5-year-old girls whose parents demonstrated an authoritative feeding style, 5-year-old girls whose parents demonstrated a responsive feeding style, 3-year-old boys whose parents demonstrated a responsive feeding style and 6-year-old boys whose parents demonstrated a neglective feeding style. Conversely, the groups with the lowest scores in toileting were 3-year-old girls whose parents demonstrated an authoritarian feeding style, 5-year-old girls whose parents demonstrated a neglective feeding style, and 3-year-old and 6-year-old boys whose parents demonstrated an authoritarian feeding style. 4. The group with the highest score in removing their coats was girls whose parnets demonstrated authoritative, responsive or neglective feeding styles. The group with the lowest score in removing their coats was boys whose parents demonstrated authoritative or neglective feeding styles.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.315-322
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• 2021

The aim of this study was to investigate the sensitivity to environmental stress, and changes in the photosynthesis capacity in Abies koreana seedlings by age and to suggest the most effective age for restoration. To identify these physiological characteristics of A. koreana, the chlorophyll fluorescence and photosynthetic capacity of 1-, 2-, 3-, 5- and 6-year-old A. koreana seedlings were observed from June 2020 to June 2021. The maximum quantum efficiency of Photosystem II (Fv/Fm), a chlorophyll fluorescence measurement parameter, was strongly positively correlated with the monthly average temperature (1-year-old seedling: r=0.8779, 2-year-old seedling: r=0.8605, 3-year-old seedling: r=0.8697, 5-year-old seedlings: r=0.8085, and 6-year-old seedlings: r=0.8316). The Fv/Fm values were the lowest in winter (November 2020-March 2021). In addition, the Fv/Fm values of 1-, 2-, and 3-year-old seedlings in winter were lower than that of 5- and 6-year-old seedlings, while the Fv/Fm values in summer were relatively higher than those in winter. Further, the Fv/Fm values of seedlings of all ages decreased in August 2020, when the monthly average temperature was the highest. In particular, 1-year-old to 3-year-old seedlings showed Fv/Fm values less than 0.8. Further, the photosynthetic capacity measured in August 2020 increased with increasing seedling age. The analysis of variance results for summer Fv/Fm values showed significant differences in age-specific averages (p<0.05), and Duncan's multiple range test showed significant differences between 5- and 6-year-old seedlings and 1-, 2-, and 3-year-old seedlings (p<0.05). These results suggested that the 5- and 6-year-old seedlings were less sensitive to environmental stress and showed better photosynthetic capacity than the 1-, 2-, and 3-year-old seedlings. Therefore, 5-year-old or older A. koreana seedlings can be used as restoration materials because they can show increased adaptability and stable growth during transplantation due to their relatively high environmental resistance and photosynthetic capacity.

• Journal of Ginseng Research
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• v.5 no.2
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• pp.114-121
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• 1981

Starch was isolated from 4-year-old and 6-year-old ginseng roots and its physical and chemical characteristics were studied. The results obtained were summarized as follows. 1. The shape of ginseng starch granules was polygonal and rounded with its granule size ranging from 2.0 to 7.5$\mu$. The swelling power of 4-year-old ginseng starch was much greater than that of 6-year old ginseng starch. Gelatinization pattern showed that 6-year-old ginseng starch gelatinized rapidly at $65^{\circ}C$, whereas 4-year-old starch continued to gelatinize, without having a definite gelatinization temperature as temperature increased 3. Amylogram of ginseng starch showed that gelatinization initiated at 61$^{\circ}C$ and was completed at 88$^{\circ}C$ with its viscosity reaching at 810 B.U. 4. The amylose contents were 32% and 9% for 4-year-old and 6-year-old ginseng starch, respectively. 5. X-ray diffraction analyses indicated that there were some structural differences between 4-year-old and 6-year-old ginseng starch.

• Applied Biological Chemistry
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• v.40 no.2
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• pp.148-151
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• 1997

Platycodon root contains abundant pharmaceutical substances and is widely used as a food and a medicinal herb. To identify the major components of the three-year-old and the 24-year-old platycodon roots cultivated in a local farm were analyzed and compared with their chemical compositions prior to their pharmaceutical substances, which were used as the fundamental data. There were no differences in chemical composition, inorganic compositions and fatty acid compositions between the 24-year-old and three-year-old roots. Total amino acid contents were 375.8 mg% and 891.6 mg% in the 24-year-old and three-year-old platycodcon roots, respectively. Sixteen species of amino acid were identified by amino acid autolyzer except cysteine both in the 24-year-old and three-year-old roots. Linoleic acid, which was the highest fatty acid contained both in the 24-year-old and three-year-old roots, was estimated to be 48.7% and 39.1% of total fatty acids, respectively.

• Journal of Sasang Constitutional Medicine
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• v.17 no.1
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• pp.84-102
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• 2005

Objectives The purpose of this study was to objectify the diagnosis of Sasang constitution. It was analyzed the adult female voices into 44 factors with PSSC-2004. Methods The study was conducted on the subjects inputted /a/ 2.5-3 sec of 217 adult women voices to PSSC-2004. The statistical analyses are applied to three groups: total group, under 55 year-old group, over 55 year-old group. The group of total 216 was composed with 8 Taeyangins, 60 Soyangins, 66 Taeumins and 83 Soeumins. The under 55 year-old group was composed with 6 Taeyangins, 53 Soyangins, 55 Taeumins and 76 Soeumins. The over 55 year-old group was composed with 2 Taeyangins, 7 Soyangins, 11 Taeumins and 7 Soeumins. Results 1. In total group and under 55 year-old one, Soeumin's center pitch were significantly high compared with the others(P=0.005)(P=0.019). It was significant for distinction between Taeumin and Soeumin at the result of post mortem. 2. In over 55 year-old group, Soeumin's center freq.(total) and center freq.(1) were significantly high compared with the others(P=0.024)(P=0.016). In under 55 year-old group, Soeumin's center freq.(4) was significantly high(P=0.025). While Taeumin's center freq.(3) in under 55 year-old group and center freq.(4) in over 55 year-old group were significantly high(P=0.049)(P=0.043). 3. In over 55 year-old group, Soeumin's APQ(center), APQ(2/5), APQ(3/5), APQ(4/5) and APQ(2/3) were significantly high compared with the others. It was significant for distinction between Taeumin and Soeumin at the result of post mortem(P=0.004) (P=0.003) (P=0.004) (P=0.013) (P=0.004). 4. In over 55 year-old group, Soeumin's APQ(1/3), APQ(1/2), APQ(2/2) and zeroover APQ were significantly high compared with the others (P=0.013) (P=0.003) (P=0.008) (P=0.012). 5. In over 55 year-old group, Taeyangin's octave 3 shimmer was significantly high compared with the others(P=0.000). 6. In under 55 year-old group, Taeyangin's total energy was significantly high compared with the others(P=0.040). 7. In under 55 year-old group, Taeyangin's zeroover energy sum was significantly high compared with the others(P=0.038). Conclusions From above result, there is the possibility of efficiency standard guide for constitutional diagnosis by analyzation of the voices.

• Korean Journal of Child Studies
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• v.25 no.5
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• pp.179-191
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• 2004

An emotion inference task was used to investigate children's cognitive cuing by age, cue type, and situation-emotion fit. Subjects were 41 of 3-, and 5-year-old children from two different day-care centers in Seoul and Kyonggi province. Each child was individually interviewed with pictorial tasks. 5-year-old children demonstrated more cuing, thinking, and application responses than 3-year-old children. Particularly, they showed more situation and thinking responses in situation-emotion match than in situation-emotion mismatch. 3-year-old children showed more past-oriented responses than 5-year-old children. In the interpersonal cue story, there were more situation, past-oriented and thinking responses than in the interobjective ones. The interpersonal cue story produced more situation responses in situation-emotion match than in situation-emotion mismatch.

• Korean Journal of Child Studies
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• v.27 no.3
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• pp.255-270
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• 2006

The present study applied the research of Endres(2003) to investigate understandings of theory of mind by 3- and 4-year-olds based on false belief, perspective taking, and intention. Participants were 86 3- and 96 4-year-old children in three kindergartens. Individual interviews were conducted for each task. Results showed that 4-year-olds scored higher than 3-year-olds on all three variables. Both 3- and 4-year-olds scored highest on perspective taking and lowest on intention. These results suggest that children's understandings of the theory of mind are still developing between 3 and 4 years of age and that their understanding of intention develops more slowly than false belief and perspective taking.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.348-353
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• 1999

Ten Australian Stock Horses colts (five yearling and five 3-year old colts) of which 2 yearlings and 2 three year old colts served as control animals while 3 yearlings and 3 three year old colts received two GnRH immunizations within 4 weeks interval were used in this study. By the 5th to 6th week after immunization, the GnRH antibody titres in the plasma rose above 1:1000 and attained peak levels of 1:6500 by the 8th week and gradually declined to about 1:3000 by the 10th week in both the age groups. The testosterone and androstenedione concentrations of the control colts in both age groups were significantly greater (p<0.05) than that of the vaccinated groups. During the immunosuppression period, the vaccinated colts behaved like geldings. Semen could not be collected from 2 of the 3 three-year old vaccinated colts. The testicular dimensions, testicular weight, parenchymal weight, seminiferous tubule volumes, interstitial space volumes, Leydig cell volume, seminiferous tubule % of the control colts were significantly greater than those of the vaccinated colts in both the age groups. The 3-year old control colts had a significantly (p<0.05) greater % of Leydig cells than the control and vaccinated 1-year old colts. There was arrest of spermatogenesis with complete absence of sperm in the testes of the vaccinated colts while there was various stages of spermatogenesis in those of the control colts. Morphometric analysis demonstrated that the 3-year old colts had significantly (p<0.05) greater DSP/gm of testis and DSP/testis than those of the 1-year old control colts. This study elucidated that the GnRH immunization could suppress the testicular function of the 3-year old and yearling colts.

• Applied Biological Chemistry
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• v.39 no.6
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• pp.517-520
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• 1996

To identify the volatiles of Platycodon roots, the three-year-old and the 24-year-old Platycodon roots cultivated in a local farm were extracted with ethyl ether by SDE method. 54.71mg% and 80.01mg% of volatile components were collected in the three-year-old and in 24-year-old Platycodon roots, respectively. As a result of GC/MS(TIC) analysis, 24 and 28 compounds were identified, respectively; The amounts of volatile components such as hexanol, trans-2-hexenol and nonanal in 24-year-old roots were detected above ten times than those in 3-year-old roots. The Volatiles of 24-year-old roots were fractionated by SCC and identified by GC/MS to be 10 hydrocarbons, 8 alcohols, 10 aldehydes, 5 esters, 6 acids and 1 unknown compound as the major components. The species and total amounts of volatile component in 24-year-old Platycodon roots were considered to be higher than those in 3-year-old Platycodon roots. *Abbreviations 1. Platycodon : platycodon grandiflorus (jacquin) A. De Candolle. 2. SDE : Simultaneous Distillation Extraction 3. SCC : Silicagel Column Chromatography 4. GC : gas chromatography 5. GC/MS : Gas Chromatography-Mass Spectrometer 6. TIC : Total Ion Chromatogoraphy