• Journal of Life Science
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• v.13 no.6
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• pp.794-798
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• 2003

Batch production of (S)-phenyl oxirane was investigated using epoxide hydrolase activity of Rhodosporidium toruloides SJ-4. Effect of reaction condition of asymmetric biohydrolysis of racemic phenyl oxirane was analyzed and optimized by response surface methodology. The optimal conditions of pH, temperature and DMSO cosolvent ratio were 7.4, $34^P\circ}C$, and 2.3%(v/v), respectively. The final yield was enhanced up to 67%, and reaction times required to reach 99% ee (enatiomeric excess) decreased down to 50% by response surface methodology Enantiopure (S)-phenyl oxirane with 100% enantiopurity and 24% yield (theoretical yield = 50%) was obtained from racemic substrate.

• Korean journal of applied entomology
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• v.20 no.3 s.48
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• pp.173-175
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• 1981

New screening method of varietal resistance by isotope $^{32}P$ was examined in these experiments. Insects were fed for 24 hrs. in the vials, with rice seedlings absorbed the solution if $0.6\~7{\mu}\;Ci\;^{32}P$ for 24 to 48 hrs. Feeding amounts of 3 species of plant-hoppers at the different stages and duration of infestation were investigated for effective process of screening method of the varietal resistance using isotope $^{32}P$. Feeding amount of $^{32}P$ of brown plant-hopper was observed for the different varieties. The results may be summarized as follows: 1. Brown planthopper fed greatest and the feeding amount were increased in order of white-backed planthopper and small brown planthopper. Female hoppers fed more than male. 2. Feeding amount was increased in order of adult, 5th instar, adult, 4th instar, 3rd instar 2nd instar and 1st instar. The duration of $24\~48\;hours$ is considered sufficient for insect infestation. 3. New screening method by $^{32}P$ was compared with seedling bulk screening method in view of feeding amount and plant reaction. Feeding amount of $^{32}P$ by brown planthopper in Milyang 47, resistant variety to this insect, was very low, while in TN 1, susceptible variety, it was very high about one hundred tines of Milyang 47.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7513-7523
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• 2015

The purpose of this study was to investigate relationships between self-care knowledge and compliance of ischemic stroke patients with diabetic. The participants were 117 ischemic stroke patients with diabetics who were outpatients at the P university hospital. Data were collected from July 1 to August 31, 2013. Data were analyzed using SPSS 18.0., specifically descriptive statistics, t-test, ANOVA, and Pearson's correlation analysis. Total mean score and correct answer ratio of self-care knowledge were 14.69 points, 63.9%. Total mean score and compliance ratio of self-care compliance were 2.24 points, 44.8%. The correlation coefficient between self-care knowledge and compliance was a significant positive correlation (r=.54, p<.001). Among sub-elements, diet(r=.24, p=.011), medication(r=.43, p<.001), living habits(r=.19, p=.042) and cautions and warnings(r=.45, p<.001) were significant positive correlations. Based on the results of this study, we need to develop integrated nursing intervention education programs for self-care knowledge and compliance promotion of ischemic stroke patients with diabetic.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10705-10711
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• 2015

Oral cancer is one of the most common and lethal cancers in the world. Combination chemotherapy coupled with nanoparticle drug delivery holds substantial promise in cancer therapy. This study aimed to evaluate the efficacy and safety of two dosages of our novel pH and temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NPs) with attention to the MMP-2 mRNA profile in a 4-nitroquinoline-1-oxide induced oral squamous cell carcinoma (OSCC) model in the rat. Our results showed that both IV and oral dosages of DOX-MTX NP caused significant decrease in mRNA levels of MMP-2 compared to the untreated group (p<0.003). Surprisingly, MMP-2 mRNA was not affected in DOX treated compared to cancer group (p>0.05). Our results indicated that IV dosage of MTX-DOX is more effective than free DOX (12 fold) in inhibiting the activity of MMP-2 in OSCCs (P<0.001). Furthermore, MMP-2 mRNA expression in the DOX-MTX treated group showed a significant relation with histopathological changes (P=0.011). Compared to the untreated cancer group, we observed no pathological changes and neither a significant alteration in MMP-2 amount in either of healthy controls that were treated with oral and IV dosages of DOX-MTX NPs whilst cancer group showed a high level of MMP-2 expression compared to healthy controls (p<0.001).Taking together our results indicate that DOX-MTX NPs is a safe chemotherapeutic nanodrug that its oral and IV forms possess potent anti-cancer properties on aggressive tumors like OSCC, possibly by affecting the expression of genes that drive tumor invasion and metastasis.

• The Korea Journal of Herbology
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• v.24 no.2
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• pp.39-48
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• 2009

Objectives : To clarify the possible effect of JS (Juglans sinensis), PCF (Psoralea corylifolia L.), and J+P(JS+PCF), we examined their influence on the development of pulmonary eosinophilic inflammation in the asthmatic murine model. Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by intraperitonial injections of 0.2 ml alum-precipitated Ag containing 100 ${\mu}$g of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 minutes/day on 3 days/week for 8 weeks (at a flow rate of 250 L/min, 2.5% ovalbumin in normal saline) and, JS, PCF and J+P (200 mg/kg, 400 mg/kg) were orally administered 3 times per week for 8 weeks. Results : The suppressive effects of JS, PCF, and J+P were demonstrated by the accumulation of eosinophils into airways, with the reduction of eosinophils and lung leukocytes. These were correlated with the marked reduction of IL-4, IL-5, IL-13 levels in the BALF and serum. OVA-specific IgE levels were also decreased in serum and BAL from these mice. And also JS, PCF, and J+P decreased eosinophilic CCR3 and CD11b expression in lung tissue. Conclusions : These results indicate that JS, PCF, and J+P have deep inhibitory effects on airway inflammation and hyper-responsiveness in the asthmatic murine model. The suppression of IL-5, IgE, and eosinophilils and the increase of IFN-${\gamma}$ production in BALF seem to contribute to these effects. Specially, esosinophils and TNF-a in J+P combination group were significantly reduced in BALF and lung tissue. Hence, the results indicated that JS, PCF, and J+P could act as an immuno-modulator which possesses anti-inflammatory and anti-asthmatic property by modulating the imbalance between Th1 and Th2 cytokines.

• Journal of Practical Engineering Education
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• v.15 no.2
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• pp.475-484
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• 2023

This study was conducted to identify the influences of bioethics awareness and patient-centered care on attitude of terminal care of hospice nurses. The participants were 145 nurses working in hospice care center of hospitals in Korea. Data were collected during May and June in 2018 and were analyzed with descriptive statistics, t-test, one way ANOVA, Pearson's correlation coefficient, and multiple hierarchecal regression analysis using SPSS 24.0 program. Attitude of terminal care was influenced by hospice specialist certification (β=.15, p=.031), bioethics awareness (β=-.24, p=.003), and patient-centered care (β=.36, p<.001) in the regression model. These variables explained 36.9% of attitude of terminal care. In order to improve the attitude of terminal care of hospice nurses, programs for improving patient-centered care competence including bioethics awareness should be provided.

• The Korean Journal of Ecology
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• v.16 no.4
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• pp.409-416
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• 1993

After spraying simulated sulphuric acid rain (SSAR) to the leaves of Glycine max, Quercus aliena var. pellucida and Pinus rigida, the leachates were consecutively collected and quantitatively determined for the concentration of K and Ca. The amount of the leached ion decreased with subsequent acid treatment for all plants. But as the pH of SSAR decreased, the amount of the leached ion increased. The cumulative quantities of K leached from each species were 1.04 to 1.46 times higher at pH 4.0, 1.09 to 1.58 times higher at pH 3.5 and 1.24 to 2.03 times higher at pH 3.0 compared with pH 5. 6 treatments. The cumulative quantities of Ca leached from each species were 1.23 to 1.47 times higher at pH 4.0, 1.50 to 1.92 times higher at pH 3.5, and 2.45 to 3.30 times higher at pH 3.0 compared with pH 5.6 treatments. The Ca /K ratio in 1000 ml leachate was 1.10 to 2.91 for Q. aliena var. pellucida and 1.68 to 2.98 for P. rigida, but 0.66 to 0.91 for G, max. The Ca /K ratio in 1000 rnl leachate increased for all three species, as the pH of SSAR decreased. Foliage analysis after acid rain treatment showed leaching effect at pH 3.0.

• Childhood Kidney Diseases
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• v.7 no.2
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• pp.150-156
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• 2003

Purpose : Efforts to predict the clinicopathological outcome of IgA nephropathy have been made but have yielded conflicting results and have not helped in deciding the appropriate timing of the renal biopsy. In this study, we reviewed the predictive factors of clinicopathological outcome for finding out the criteria of renal biopsy timing of IgA nephropathy. Methods : Forty children diagnosed with biopsy proven IgA nephropathy at Wonju Christian Hospital were studied retrospectively, based on medical records. Results : Among 39 patients, 2 children progressed to higher serum creatinine level. One of them reached to the end stage renal disease within 2 year 7 months. According to WHO histopathological classification, there were 15 cases of class I, 14 cases of class II, 7 cases of class III, and 3 cases of class IV. In the mild histological classes(class I, II), gross hematuria was shown in 23 out of 29 children(P=0.02). In the severe histological classes(class III, IV), gross hematuria was noted in 4 out of 10(P>0.05). The tubulointerstitial changes were grade 1 in 24 cases, grade 2 in 4 cases, grade 3 in 8 cases, and grade 4 in 3 cases. With an increase in the tubulointerstitial grade, the 24 hour urine protein/albumin ratio increased. Serum creatinine less than 0.79 mg/dL could predict the lower grade(grade 1 and 2) of tubulointerstitial changes. But serum creatinine greater than 1.13 mg/dL could predict the higher grade(grade 3 and 4) of tubulointerstitial changes. In children with gross hematuria(n=27), serum creatinine was lower(0.78 vs 1.09 mg/dL, P=0.027), serum IgA was higher(316.3 vs 198.8 mg/dL), and the cases of lower WHO classification(I and II) were more common(23 vs 4, P=0.029) than the children with microscopic hematuria. Conclusion : Serum creatinine less than 0.79 mg/dL, macroscopic hematuria, and higher 24 hour urine protein/albumin ratio would predict the lower grade glomerulo tubulointerstitial lesion in IgA nephropathy and could be used as the criteria delaying the renal biopsy.

• Proceedings of the KAIS Fall Conference
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• 2010.11b
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• pp.988-991
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• 2010

본 연구에서는 고등학교 단거리 선수의 심폐능력, 신체조성, 그리고 각 부위별 근력이 24주간 운동을 통하여 어떠한 변화가 있는지 규명하였다. D 고등학교에 재학중이며 운동경력이 최소 4년이상된 남자 단거리 선수 8명을 연구대상자로 선정하였다. 운동 전 후 연구대상자들의 심폐능력, 신체조성, 그리고 근력을 측정하여 자료처리 하였다. 본 연구결과, 체지방률, 체지방량, 제지방량, 그리고 체수분량 모두 훈련 후 통계적으로 유의한 증가가 있었다(p<0.05). $VO_2$max와 aerobic threshold는 운동전보다 향상되었으며 통계적으로 매우 유의한 변화가 나타났다 (p<0.05). 근력 또한 통계적으로 유의한 상승 변화을 보였다 (p<0.05.) 본 연구결과 24주간의 훈련 프로그램은 남자 고등학교 단거리 선수들의 총체적 체력 향상을 도모하였다. 단거리 훈련 프로그램 작성 시 전통적인 방법으로 훈련을 계획하고 실천하기 보다는 경기력 향상 및 부상 예방을 위하여 훈련 전 종합적인 신체검사 및 체력 검사를 전문가와 같이 실시 및 평가하여 이를 토대로 개인의 체력에 맞게 훈련 프로그램을 계획 실천할 필요가 있을 것이다.

• The Korean Journal of Emergency Medical Services
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• v.20 no.3
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• pp.85-93
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• 2016

Purpose: The purpose of this study was to investigate the education retention effect of endotracheal and laryngeal tube intubation using a manikin study. Methods: The study consisted of measuring intubation time, intubation success rate, and confidence of intubation after education. The evaluation of the education was performed 2 weeks, 4 weeks, and 24 weeks after education and skill tests. The study subjects were 48 paramedic students of third and fourth grade. Results: There was no significant difference in endotracheal intubation time but the time spent performing laryngeal tube intubation significantly increased over time (p<.000). The intubation success rate of endotracheal and laryngeal tube intubation was 100% in the $24^{th}$ week, and there was no significant difference in time spent performing the intubation. The students' confidence in endotracheal (p<.023) and laryngeal tube intubation (p<.001) decreased significantly from the second week to the $24^{th}$ week. Conclusion: This study revealed that it is necessary to spend at least 24 weeks to train students endotracheal and laryngeal intubation to improve the students' confidence in performance of intubation.