• Genomics & Informatics
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• v.7 no.3
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• pp.152-158
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• 2009

Although the genetic basis for bone mineral density (BMD) has been studied by many groups so far, genes responsible for this complex trait has not been completely revealed. In order to localize quantitative trait loci (QTLs) for BMD variation in Asian population, the study was designed using a group of Mongolian population, a genetically closed population with a homogeneous lifestyle. BMD was measured at the left and right wrists and ankles using DEXA in 1,082 participants from 142 families. Genotyping of 13 polymorphic microsatellite markers on chromosome 13 (average spacing 8-9 cM) and two-point and multipoint linkage analysis were performed. In two-point linkage analysis, we identified two markers, D13S175 (6.03 cM) and D13S265 (68.73 cM) that had LOD scores greater than 1 for left ankle (LOD=2.09, LOD=1.49, respectively). We also found a marker D13S175 (6.03 cM) with a high LOD for left wrist (LOD=1.49) and the markers D13S265 (68.73 cM) and D13S217 (17.21 cM) for the right wrist (LOD= 1.82, LOD= 1.62, respectively). Among these significant marker regions, only two regions at 17 cM (13p11) and 65 cM (13q21) for the right wrist overlapped with major QTLs reported in following multipoint linkage analysis (LOD= 1.7549, LOD=1.4462, respectively). This study provides the possible evidence of the presence of QTLs affecting right wrist BMD in Mongolian populations on 13p11 and 13q21. Modest evidence was also found for genes affecting left ankle and left wrist BMD on 13p13.

• Women's Health Nursing
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• v.7 no.4
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• pp.631-641
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• 2001

This study is to investigate women have pre menstruation discomfort, The period of this research was almost one month (from 25, August 2001 to 25, October 2001). The PAF (Premenstrual assessment form), which was made by Park, Myonng-Wha, used for this study. The confidence rate is Crombach's $\alpha$= .9773. Correcting research papers are analyzed by SPSS 7.5 program and normal feature of the subject is divided by frequency, percentage, mean, standard deviation, t-test and ANOVA. The results of this study are as follows; 1. The average age of the subjects is 26.47 age. 220 subjects(44.6%) have gastroenteric trouble like, enteritis, stomachache, laxity. 278 subjects usually have caffeine drink in routine life. 123 subjects(24.9%) have controlled their diet. The average age of the first plowing is 13.55 ages and distribution is between 10 years and 20 years. The average of the uncomfortable point is 2.84. The way to reduce the uncomfortable feeling on the menstruation is taking relax time. 2. All subjects have experienced pre menstruation discomfort, even the degree uncomfortable feeling is different. Through eighteenth categories on the research papers, average point 2.21 were calculated and many subjects have fatigue ($2.89{\pm}1.0614$) and normal physical uncomfortable ($2.84{\pm}1.0633$) and undercurrent water symptom and unsuitability ($2.45{\pm}0.9857$). 3. According to the result of this study, that shows statistical difference; age (F=2.56. p=.037). marriage (t=2.60, p=.009) the number of children(t=2.83, p=.005), stress level(F=13.03, p=.000) job(F=3.91, p=.020). monthly income(t=2.14. p=.032) uncomfortable degree on the menstruation (F=19.121, p=.000), use of contraceptive appliance or pill(t=-2.20, p=.043), schooling (F=3.30, p=.038). Further research is need to understand pie menstruation discomforts, the variables associated with them. And nursing intervention has to considered in program to reduces of pre-menstruation discomforts.

• Economic and Environmental Geology
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• v.35 no.2
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• pp.103-112
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• 2002

Despite the ecological importance of potentially phytotoxic $Al_{13}$-tridecamer and its formation in the simulated condition, it was not recognized in the natural soil environment. Here we performed thermodynamic calculations to examine the stability condition of $Al_{13}$-tridecamer based on the solubility of AI in the Bo horizon of Andisols, Jeju Island, dominantly composed of AI-containing solid phases such as $Al(OH)_{3}$, proto-imogolite and/or imogolite. We have found that $Al(OH)_{3}$, proto-imogolite and/or imogolite may control Al solubility in the moderate acid condition. It means that AI total activity of the soil solution equilibrated with these solid phases ranges from $10^{-6}$ ~ $10^{-8}$M in the pH 5 to 7. Calculations based on the thermodynamic data strongly indicate that the formation of $Al_{13}$-tridecamer closely related to the total activity of AI in the system. For example, for the formation of $Al_{13}$-tridecamer of $10^{-5}$M, Al total activity of $3{\times}10^{-3}$M are needed at pH 4, and $2{\times}10^{-5}$M in the pH 5 to 7. Therefore, this research and the thermodynamic consideration suggest strongly that $Al_{13}$-tridecamer should be negligible in natural soils, especially Andisols and Spodosols, These mainly contain $Al(OH)_{3}$, proto-imogolite and/or imogolite, which could prevent the formation of $Al_{13}$-tridecamer by controlling the AI total activity low. It means that the toxicity of $Al_{13}$-tridecamer with the increase of soil acidification may be considered to be definitely low.

• Microbiology and Biotechnology Letters
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• v.19 no.6
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• pp.575-582
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• 1991

To increase the production of glutathione by the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were isolated and cloned. To clone a gshI gene, the GS903 mutant strain, which is deficient in $\gamma$-glutamylcysteine synthetase activity, has been raised. A gshI gene was cloned using pBR322 plasmid as a 3.6 Kb PstI DNA fragment isolated from E. coli K-12 chromosomal DNA. Also a gshIl gene was cloned using pUC13 plasmid as a 2.2 Kb PstI-BamHI DNA fragment. To study the effects of plasmid copy number and passenger DNA size on the expression levels of the gsh genes, various recombinant plasmids containing different sets of genes were constructed. The expression levels of the gsh genes were increased approximately twice higher in pUC series plasmids than that in pBR322 plasmid. But the sizes of the passenger DNA containing the gsh genes in the vector plasmid did not affect on the expression levels of the gsh genes.

• Journal of Genetic Medicine
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• v.8 no.2
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• pp.119-124
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• 2011

Purpose: Supernumerary marker chromosome (SMC) could be associated with various phenotypic abnormalities based on the chromosomal origin of SMCs. The present study aimed to determine the genomic contents of SMCs using chromosomal microarray and to analyze molecular cytogenetic characterizations and clinical phenotypes in patients with SMCs. Materials and Methods: Among patients with SMCs detected in routine chromosomal analysis, SMCs originating from chromosome 15 were excluded from the present study. CGH-based oligonucleotide chromosomal microarray was performed in 4 patients. Results: The chromosomal origins of SMCs were identified in 3 patients. Case 1 had a SMC of 16.1 Mb in 1q21.1-q23.3. Case 2 showed 21 Mb gain in 19p13.11-q13.12. Case 3 had a 4.5 Mb-sized SMC rearranged from 2 regions of 2.5 Mb in 22q11.1-q11.21 and 2.0 Mb in 22q11.22-q11.23. Conclusion: Case 1 presented a wide range of phenotypic abnormalities including the phenotype of 1q21.1 duplication syndrome. In case 2, Asperger-like symptoms are apparently related to 19p12-q13.11, hearing problems and strabismus to 19p13.11 and other features to 19q13.12. Compared with cat-eye syndrome type I and 22q11.2 microduplication syndrome, anal atresia in case 3 is likely related to 22q11.1-q11.21 while other features are related to 22q11.22-q11.23. Analyzing SMCs using high-resolution chromosomal microarray can help identify specific gene contents and to offer proper genetic counseling by determining genotype-phenotype correlations.

• Culinary science and hospitality research
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• v.12 no.3 s.30
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• pp.119-133
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• 2006

This study was conducted to examine the sensory characteristics and the experimental of the medicinal demi-glace with varying quantity of omija extracts added. First, according to the result of this study, demi-glace sauces with varying quantity of omija extracts added were as follows; (1) pH : The pH of control groups was 5.13 and omija sauces ranged from pH 4.18 to pH 4.81. And the more omija extracts were added, the less pH was found. (2) $^{\circ}Brix$ : The control was 10.2 and omija sauces ranged from 11.1 to 13.0 $^{\circ}Brix$. The more omija extracts were added, the higher $^{\circ}Brix$ was found. (3) Color : The heavier weight of omija were added, the less L value was found; however, a, b values were increased. Secondly, the results of sensory evaluation based on authentic tastes of demi-glace sauces with the different levels of omija added were as follows; In terms of aftertastes and overall acceptability preference of demi-glace sauces, males and females preferred 2% added omija sauces. There were no significant differences in gender, but there were significant differences in sample sauce(p<0.001). This study found that 2% sauce was recommended as the best for the application of medicinal demi-glace sauce with omija.

• The Korean Journal of Health Service Management
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• v.13 no.2
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• pp.121-133
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• 2019

Objectives: The purpose of this study was to determine the effectiveness of hand acupressure in relieving shoulder pain among male manufacturing workers. Methods: A nonequivalent control group was employed using a nonsynchronized design. Data were collected from two manufacturing plants located in metropolitan area U. The data were analyzed with a ${\chi}^2$-test and an independent t-test to perform a homogeneity test, and with repeated ANOVA measures to perform an hypothesis test using the SPSS 21.0 program. Results: Hypothesis 1 that "the experimental group provided with hand moxibustion would score lower for low shoulder pain than the control group" was supported (F=306.05, p<.001). Hypothesis 2, that "the experimental group provided with hand moxibustion would have a wider range of motion than the control group" was also supported with increased flexion(F=7.73, p<.001), extension(F=7.89, p=.001), abduction(F=21.23, p<.001), and horizontal abduction(F=20.89, p=.028). Hypothesis 3, that "the experimental group provided with hand moxibustion would score lower for quality of sleep than the control group" was not supported (F=56.10, p=.091). Conclusions: Hand moxibustion is effective in relieving shoulder pain for male manufacturing workers, in relieving low shoulder pain and increasing the range of motion.

• Journal of Species Research
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• v.13 no.3
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• pp.340-351
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• 2024

A new species of the canuellid genus Brianola Monard, 1926 (Copepoda: Harpacticoida) was collected during a meiofauna survey in July 2021 at the intertidal muddy sand of Phuket Island, Thailand. Currently, there are only eight known species of Brianola: B. stebleri, B. exigua, B. curvirostris, B. elegans, B. sydneyensis, B. vangoethemi, B. hamondi, and B. haliensis. Thus, the Brianola rawaiensis sp. nov. from Thailand is the ninth species of the genus. The new species, Brianola rawaiensis is most closely related to B. haliensis by sharing a four-segmented antennule in females and five-segmented antennule in males, eight-segmented antennary exopod and three-segmented antennary endopod, and P1 exp-3 with 5 spines/setae. However, B. rawaiensis sp. nov. is different from B. haliensis by having: 1) P1 exp-1 with 1 outer pinnate spine, 2) P1 exp-2 with inner plumose seta, 3) P2 exp-3 with 3 setae/spines, 4) P2 enp-1 with one inner plumose seta, 5) P4 enp-1 with inner seta. Sexual dimorphism is expressed in the antennule and segmentation of the urosome. Ovigerous female bearing one egg sac with 20 eggs.

• YAKHAK HOEJI
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• v.38 no.5
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• pp.488-495
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• 1994

To evaluate the feasibility of transmucosal delivery of leucine enkephalin (Leu-Enk) and its synthetic analog, $[D-Ala^2]$-leucine enkephalinamide (YAGFL), their physicochemical stabilities in aqueous buffered solutions were first investigated using a stability indicating high performance liquid chromatography. The degradation of Leu-Enk and YAGFL followed the pseudo-first-order kinetics. From the pH-rate profiles, it was found that the maximal stability of the two pentapeptides was at the pH of about 5.0. The shelf lives $(t_{90%})$ for the degradation of Leu-Enk and YAGFL at pH 5.0 and $40^{\circ}C$ were found to be 48.13 and 50.9 days, respectively. From the temperature dependence of the degradation, activation energies for Leu-Enk and YAGFL were calculated to be 13.61 and 13.47 kcal/mole, respectively. A higher ionic strength and a higher initial peptide concentration in buffered solution slowed the degradation of the two pentapeptides. The addition of 2-hydroxypropyl-${\beta}$-cyclodextrin into the peptide solution did not affect the stability significantly.

• The Korean Journal of Zoology
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• v.21 no.2
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• pp.67-75
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• 1978

The auther investigated the distribution and seasonal variations of Copepods and Branchiopods in Myeongam Lake located in Cheongju City, Chungchungbuk Do. Air and Water temperature, pH, and the amount of dissolved oxygen were also measured eleven times from July 29 to November 5, 1977. The results obtained are as follows: 1. The mean values of air and water temperature, pH, and dissolved oxygen during the investigation period were $25^{\circ}C , $23.7^{\circ}C, 6.86, and 10.75 ppm, respectively. 2. The total number of species collected in this lake were 13 species (1) thermocyclops taihokuensis (Harada) (2) Thermocyclops hyalinus (Rehberg) (3) Mesocyclops leuckarti (Claus) (4) Diacyclops disjunctus (Thallwitz) (5) Paracyclops fimbriatus (Fischer) (6) Cyclops sp. a (7) Cyclops sp. b (8) Diaphanosoma brachyurum (Lievin) (9) Daphnia pulex Leydig (10) Daphnia longispina (O. F. Muller) (11) Daphnia sp. (12) Moina dubia De Guerne et Richard (13) Chydorus sphaericus (O. F. Muller) 3. Among these species, 4 species (1, 3, 8, 10) were collected continually throughout the investigation period. 4. The dominant species was Diaphanosoma brachyurum during the investigation period.