• Title/Summary/Keyword: 2b-site

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Stochastic ground-motion evaluation of the offshore Uljin Earthquake (울진앞바다 지진( '04. 5. 29, M=5.2)의 추계학적 지진동 평가)

  • Yun, Kwan-Hee;Park, Dong-Hee;Choi, Weon-Hack;Chang, Chun-Jung
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2005.03a
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    • pp.18-25
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    • 2005
  • Stochastic ground-motion method is adopted to simulate horizontal PGA values for the offshore Uljin earthquake recorded at nationwide seismic stations. For this purpose, the Fourier spectra are calculated at every stations based on comprehensive results of wave propagation and site effect which were previously revealed through inversion process applied to large accumulated spectral D/B. In addition, the apparent source spectrum of the offshore Uljin earthquake is estimated by removing the path and site response from the observed spectra. The distance dependent time-duration model is revised by iteratively fitting the PGA values generated by using the raw spectra data to the observed PGA data. The stochastic ground-motion method predicts the observed PGA values within a error of ${\sigma}_{log10}=0.1$. Transfer functions of a site relative to another site are estimated based on the error residual of the inversion results and used to convert PGA values at multiple stations to expected PGA values at a reference station of TJN. The converted PGA values can be used as basic data to evaluate the ground-motion attenuation relations developed for seismic hazard analysis that concerns the large damaging earthquakes.

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Biochemical Characterization of Oligomerization of Escherichia coli GTP Cyclohydrolase I

  • Lee, Soo-Jin;Ahn, Chi-Young;Park, Eung-Sik;Hwang, Deog-Su;Yim, Jeong-Bin
    • BMB Reports
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    • v.35 no.3
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    • pp.255-261
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    • 2002
  • GTP cyclohydrolase I (E.C. 3.5.4.16) is a homodecameric protein that catalyzes the conversion of GTP to 7,8-dihydroneopterin triphosphate (H2NTP), the initial step in the biosynthesis of pteridines. It was proposed that the enzyme complex could be composed of a dimer of two pentamers, or a pentamer of tightly associated dimers; then the active site of the enzyme was located at the interface of three monomers (Nar et al. 1995a, b). Using mutant enzymes that were made by site-directed mutagenesis, we showed that a decamer of GTP cyclohydrolase I should be composed of a pentamer of five dimers, and that the active site is located between dimers, as analyzed by a series of size exclusion chromatography and the reconstitution experiment. We also show that the residues Lys 136, Arg139, and Glu152 are of particular importance for the oligomerization of the enzyme complex from five dimers to a decamer.

Database Modeling and Environmental Information for a Radioactive Waste Repository Site

  • Park S. M.;Rhee C. G.;Park J. B.;Lee H. J.;Kim Chang Lak
    • Nuclear Engineering and Technology
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    • v.36 no.3
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    • pp.263-275
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    • 2004
  • For the safe management of nuclear facilities, including a radioactive waste repository, data about the facility site and the surrounding environment must be collected and managed systematically. This is particularly true for a radwaste repository, which has to be institutionally controlled for a long period after closure. The objectives of this study are (1) to establish a systematical management plan for information about a radwaste repository site and its environment, and (2) to design a database management program for this information, based on the Relative Database Management System (RDBMS). The spatial data are designed by the geodatabase, which is a new object, based on the RDBMS, to manage spatial information related to the database. To meet this requirement, a new program called 'Site Information and Total Environmental data management System (SITES)' is being developed. The scope that produced from the first step of the present study for development of the SITES is introduced. The database is designed to combine spatial and attribute data, and is designed for the establishment of the Geographic Information System (GIS). The hardware and software systems are designed with consideration given to the total data management of the items within the radioactive environment.

Site specific ground motion simulation and seismic response analysis for microzonation of Kolkata

  • Roy, Narayan;Sahu, R.B.
    • Geomechanics and Engineering
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    • v.4 no.1
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    • pp.1-18
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    • 2012
  • The spatial variation of ground motion in Kolkata Metropolitan District (KMD) has been estimated by generating synthetic ground motion considering the point source model coupled with site response analysis. The most vulnerable source was identified from regional seismotectonic map for an area of about 350 km radius around Kolkata. The rock level acceleration time histories at 121 borehole locations in Kolkata for the vulnerable source, Eocene Hinge Zone, due to maximum credible earthquake (MCE) moment magnitude 6.2 were generated by synthetic ground motion model. Soil investigation data of 121 boreholes were collected from the report of Soil Data Bank Project, Jadavpur University, Kolkata. Surface level ground motion parameters were determined using SHAKE2000 software. The results are presented in the form of peak ground acceleration (PGA) at rock level and ground surface, amplification factor, and the response spectra at the ground surface for frequency 1.5 Hz, 3 Hz, 5 Hz and 10 Hz and 5% damping ratio. Site response study shows higher PGA in comparison with rock level acceleration. Maximum amplification in some portion in KMD area is found to be as high as 3.0 times compared to rock level.

An Effect of Safety Leadership Coaching Program on Safety Behaviors of Construction Workers: Based on Behavior Based Safety (안전 리더십 코칭 프로그램이 건설 현장 근로자들의 안전 행동에 미치는 효과 : 행동기반 안전관리(Behavior Based Safety: BBS)를 중심으로)

  • Lee, Jidong;Oah, Shezeen;Moon, Kwangsu
    • Journal of the Korean Society of Safety
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    • v.33 no.6
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    • pp.115-122
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    • 2018
  • This study examined the effect of safety leadership coaching program on managers' safety management and workers' safety behaviors in construction site. Three or four managers and about one hundred workers at each site participated in this study. Safety leadership coaching program consisted of safety leadership education, goal setting, self-monitoring and feedback on workers' safety behavior, reward for three safety management behaviors; (1) safety observations of workers safety behavior and (2) providing positive feedback on safe behavior (3) providing corrective feedback on risk behavior and daily safety education for workers. Dependent variables were the percentage of safe behaviors of workers and frequency of managers' safety management behaviors. A nonconcurrent AB multiple baseline design across settings was adopted. After baseline(A), safety leadership coaching program (B) was introduced to each site. The results showed that safety leadership coaching program was effective to increase managers' and workers' safety behaviors. These results suggest that safety leadership coaching program developed in this study would be an alternative treatment technique to improve construction safety management. In addition, the implications, limitations of this study, and future studies are discussed.

Kinetic Study on Dephosphorylation of Myelin Basic Protein by Some Protein Phosphates

  • 황인성;김진한;최명운
    • Bulletin of the Korean Chemical Society
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    • v.18 no.4
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    • pp.428-432
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    • 1997
  • The dephosphorylation specificity of protein phosphatase 2A (PP2A), calcineurin (PP2B) and protein phosphatase 2C (PP2C) were studied in vitro using myelin basic protein (MBP) as a model substrate which was fully phosphorylated at multiple sites by protein kinase C (PKC) or cyclic AMP-dependent protein kinase (PKA). In order to determine the site specificity of phosphates in myelin basic protein, the protein was digested with trypsin and the radioactive phosphopeptide fragments were isolated by high performance liquid chromatography (HPLC) on reversed-phase column. Subsequent analysis and/or sequential manual Edman degradation of the purified phosphopeptides revealed that Thr-65 and Ser-115 were most extensively phophorylated by PKA and Ser-55 by PKC. For the dephosphorylation kinetics, the phosphorylated MBP was treated with calcineurin or PP2C with various time intervals and the reaction was terminated by direct tryptic digest. Both Thr-65 and Ser-115 residues were dephosphorylated more rapidly than any other ones by phosphatases. However it can be differentiated further by first-order kinetics that the PP2B dephosphorylated both Thr-65 and Ser-115 with almost same manner, whereas PP2C dephosphorylated somewhat preferentially the Ser-115.

Neutron diffraction and Massbauer studies on $Sr_2Fe_{1-x}Cr_xMoO_6$

  • 유홍주;김성백;심인보;김철성;최용남;오화숙
    • Proceedings of the Korean Magnestics Society Conference
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    • 2002.12a
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    • pp.106-107
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    • 2002
  • 상온 거대자기저항 물질로 많은 연구가 이루어지고 있는 double perovskite (A$_2$BB'O$_{6}$) 계 물질은 B-site의 전이금속 윈자 종류에 따라 다양한 특성이 나타나는 것으로 보고되고 있다$^{[1]-[4]}$ . 그 중 Sr$_2$FeMoO$_{6}$ 시료가 MR 특성 및 전자기적 특성이 가장 우수한 것으로 알려져 있으나, Figure 1에 보이는 것과 같이, Sr$_2$CrMoO$_{6}$ 시료가 가장 높은 전이온도를 갖음으로 훌륭한 소자로의 응용 가능성을 보이고 있다. (중략)

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A Study on the B-to-B Order Processing System in Supply Chain Management Environment (SCM 환경에서 기업 간 수주처리시스템에 대한 연구)

  • Seo, Jun-Yong;Koh, Jae-Moon;Park, Hee-Cheon
    • IE interfaces
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    • v.13 no.3
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    • pp.416-423
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    • 2000
  • This paper considers a web-based order processing system under SCM environment. Following the framework of B2B electronic commerce, we focus on activities closely related to order handling within the supply chain. We also try to integrate the order processing system with the overall information system of the company. The methodology is applied to the case of H company and a prototype system is designed. This study analyzes the current product estimate and order handling process in the point of view of both the purchasing and the selling companies. It intends improvement by changing the existing closed way of request and offering estimate through fax or VAN into an open way using internet. For this, a company should be registered on the order site of internet. Then it can request estimate, take estimate, and handle order through internet adopting the concept of electrical commercial transaction.

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Cloning and Expression of B. Aphaericus insecticidal toxin gene in E. coli

  • Lim, Pyong-Ok;Lee, Hong-Sup;Lee, Hyung-Hoan
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.515.2-515
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    • 1986
  • B. sphaericus 1593 K-5 synthesize a potent entomicidal toxin against mosquito larvae. B. sphaericus EcoRl DNA fragment carrying the biocidal activity was clonied and expression in E. coli JM83. For the construction of a recombinant plasmid bearing the toxin activity the DNA of B. sphaericus was partially digested by the EcoRl. The EcoRl DNA fragments were ligated to plasmid pUC 8-EcoR1 site. The transformants were selected on LB plates containing X-gall and ampicilline. The transformants were bioassayed against mosquito larvae of which two clones showed biocidal activity. The two clones were redigested with Eco R1 and analyzed by 0.7% agarose gel electrophorsis.

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