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Environment of Fluvial Sedimentary Deposits and Palynological Occurrence in the Geochang Area (거창 지역 하성퇴적층 형성환경과 화분산상 연구)

  • Kim, Ju-Yong;Yang, Dong-Yoon;Bong, Pil-Yun;Kim, Jin-Kwan;Oh, Keun-Chang;Choi, Don-Won
    • The Korean Journal of Quaternary Research
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    • v.20 no.1 s.26
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    • pp.39-50
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    • 2006
  • Jeonjangri site of Geochang area is located in the Geochang Basin, and lies on the river terrace of upstream part of Hwang River. Fluvial deposits are well distributed at the northern and southern walls of trench 2(district 2) in the Jeonjangri archeological site. This study aims to interpret the occurrences of fluvial sedimentary deposits on the basis of grain size analysis and palynological analysis in the representative sections of Jeongjangri site. The sedimentary profile shows that the upper units are typified by paleosols with soil wedge formed at about $25,000{\sim}30,000yr$ B.P, and the lower units are characterized by reddish brown muddy sands, organic muds and sand/gravel downwards in the profile. Particularly palynological study on the organic muds of southern wall section showed a result that lower unit is dominant with grass vegetation, and upper unit with Alnus-Quercus-Pinus vegetation. The former is interpreted to be formed at $60,000{\sim}50,000yr$ B.P (stadial), while the latter at $80,000{\sim}70,000yr$ B.P. In general broad-leaved/coniferous mixed forests are mostly dominant in Jeongjangri site and the climate was presumed to be cool temperate at that time.

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Site-directed Mutagenesis Analysis Elucidates the Role of 223/227 Arginine in 23S rRNA Methylation, Which Is in 'Target Adenine Binding Loop' Region of ErmSF (위치 지정 치환 변이를 이용한 ErmSF의 '타깃 Adenine Binding Loop'을 형성하는 부위에 존재하는 223/227 Arginine 잔기의 23S rRNA Methylation 활성에서의 역할 규명)

  • Jin, Hyung-Jong
    • Korean Journal of Microbiology
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    • v.48 no.2
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    • pp.79-86
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    • 2012
  • ErmSF is one of the Erm family proteins which catalyze S-adenosyl-$_L$-methionine dependent modification of a specific adenine residue (A2058, E. coli numbering) in bacterial 23S rRNA, thereby conferring resistance to clinically important macrolide, lincosamide and streptogramin B ($MLS_B$) antibiotics. $^{222}FXPXPXVXS^{230}$ (ErmSF numbering) sequence appears to be a consensus sequence among the Erm family. This sequence was supposed to be involved in direct interaction with the target adenine from the structural studies of Erm protein ErmC'. But in DNA methyltarnsferase M. Taq I, this interaction have been identified biochemically and from the complex structure with substrate. Arginine 223 and 227 in this sequence are not conserved among Erm proteins, but because of the basic nature of residues, it was expected to interact with RNA substrates. Two amino acid residues were replaced with Ala by site-directed mutagenesis. Two mutant proteins still maintained its activity in vivo and resistant to the antibiotic erythromycin. Compared to the wild-type ErmSF, R223A and R227A proteins retained about 50% and 88% of activity in vitro, respectively. Even though those arginine residues are not essential in the catalytic step, with their positive charge they may play an important role for RNA binding.

Post LGM Fluvial Environment and Palynological Changes of South Korea

  • Kim, Ju-Yong;Yang, Dong-Yoon;Bong, Pil-Yoon;Nahm, Wook-Hyun;Lee, Heon-Jong;Lee, Yung-Jo;Hong, Sei-Sun;Lee, Jin-Young;Kim, Jin-Wkan;Oh, Keun-Chang
    • The Korean Journal of Quaternary Research
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    • v.17 no.2
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    • pp.17-23
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    • 2003
  • In Korea terrestrial fluvial sequences can be used as pedological and sedimentological markers indicating a millenium-scale environmental and climatic changes imprinted in fluvial sub-environments, which in turn are represented by the cyclicity of fluvial sands, backswamp organic muds, and flooding muds intercalations of frostcracked or dessicated brown paleosols. Post LGM and Holocene fluvial and alluvial sedimentary sequences of Korea are formed in such landscapes of coastal, floodplain, backswamp and hillslope areas. Among them, the most outstanding depositional sequences are fluvial gravels, sands and organic mud deposits in coastal, fluvial, or alluvial wetlands. The aim of this study is to explain the sedimentary sequences and palynofloral zones since the last 15,000years, on the basis of organic muds layers intercalated in fluvial sand deposits. Jangheung-ri site of Nam river, Soro-ri site of Miho river, Youngsan rivermouth site in Muan, Oksan-ri site of Hampyeong and Sanggap-ri site of Gochang are illustrated to interpret their sedimentary facies, radiocarbon datings, and palynofloral zonation. Up to the Middle to Late Last Glacial(up to 30-35Ka), old river-bed, flooding, and backswamp sequences contain such arboreal pollens as Pinus, Abies, and Picea, and rich in non-arboreal pollens like Cyperaceae, Gramineae, Ranunculaceae, and Compositae. During the LGM and post-LGM periods until Younger Dryas, vegetation has changes from the sub-alpine conifer forest(up to about 17-11Ka), through the conifer and broad-leaved deciduous forest, or mixed forest (formed during 16,680-13,010yrB.P), to the deciduous and broad-leaved forest (older than 9,500yrB.P). In the Earliest Holocene flooding deposits, fragments of plant roots are abundant and subjected to intensive pedogenic processes. During Holocene, three arboreal pollen zones are identified in the ascending order of strata; Pinus-Colyus zone(mixed conifer and deciduous broad-leaved forest, about up to 10Ka), Alnus-Quercus forest (the cool temperate deciduous broad-leaved forest, about 10Ka-2Ka), and Pinus forest (the conifer forest, about after 2Ka), as examplified in Soro-ri site of Cheonwon county. The palynological zonations of Soro-ri, Oksan-ri, Sanggap-ri, Youngsan estuary, and Gimhae fluvial plain have been recognized as a provisional correlation tool, and zonations based on fluvial backswamp and flooding deposits shows a similar result with those of previous researchers.

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The Analysis of Web Sites of Textile Exchange of B to B (기업 대 기업간(B to B) 섬유거래 웹사이트 분석)

  • 홍병숙;이은진;이지연
    • Journal of the Korean Society of Clothing and Textiles
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    • v.27 no.1
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    • pp.123-133
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    • 2003
  • The specific objectives of the study were as follows: 1) To investigate the composition system (design, usability and interactivity) of web sites of textile exchange of B to B 2) To examine and valuate contents and marketing (announcement, satisfaction and variety of contents) of web sites of textile exchange of B to B. The data were collected from search engine, portal sites of evaluation, direct contact, interview over the phone with web master of concerned web sites and the result of analytical valuation of web sites. The results of this study were as fellows: 1) The Dongsung trading intended to mainly use their homepage as a inside communication place by intranet network. The Daechang trading was mainly using their homepage as a tool of expansion of their outside export market. The etextiler was selling their web solutions through homepage. The texcom was offering the web place and useful informations to trading companies in Asia. 2) The texcom consisted text with little image to speed up for loading and navigation for usability of users. The Dongsung trading made intranet network for communication and exchange of informations of company inside. The etextiler offered a booking menu to inquiry in homepage. The Daechang trading tried to give good impression from the introduction page at homepage.

Structural and Microwave Dielectric Properties of $La(Mg_{1/2}Ti_{1/2})O_3$ Ceramics ($La(Mg_{1/2}Ti_{1/2})O_3$ 세라믹스의 구조 및 고주파 유전 특성 연구)

  • Yeo, Jae-Hyun;Baek, Jong-Hu;Nham, Sahn;Lee, Hwack-Joo;Park, Hyun-Min;Byun, Jae-Dong
    • Korean Journal of Materials Research
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    • v.9 no.9
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    • pp.943-947
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    • 1999
  • The crystal structure and the microwave dielectric properties of $La(Mg_{1/2}Ti_{1/2})O_3$ ceramics have been investigated. $La(Mg_{1/2}Ti_{1/2})O_3$ has the 1:1 ordered monoclinic structure with the lattice parameters of $a = 5.5467(3){\AA}, b = 5.5616(3){\AA}, c = 7.8426(5) {\AA} and \beta = 89.9589 (2)^{\circ}$ The spacegroup of LMT is $P2_1/n$. Monoclinic LMT has the in­phase and anti-phase tilting of octahedra with the $a^_a^_c^_$ tilting system. Anti-parallel shift of A-site cations was also found in LMT. The relative density of the specimens sintered above $1600^{\circ}C$ was ranged between 95 % and 96 % of the theoretical density and the dielectric constant of specimens was about 28. The highest $Q\timesf$ achieved in this investigation was 63,100 for the specimen sintered at $1630^{\circ}C$ for 5 hr. Temperature coefficient of resonance frequency ranged from $>-74 ppm/^{\circ}C ~ -79 ppm/^{\circ}C$.

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Electronic Structure and Magnetism of Alloying Elements Substituted B2 FeAl Intermetallic Compounds: A Density Functional Study

  • Yun, Won Seok;Lee, Jee Yong;Kim, In Gee
    • Proceedings of the Korean Magnestics Society Conference
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    • 2012.11a
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    • pp.130-131
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    • 2012
  • In this study, the thermodynamic and magnetic properties of alloying element substituted B2 FeAl systems have been investigated using the all-electron FLAPW method based on the GGA. It was shown that the important changes take place in the structural properties as well as in the magnetism when alloying element is substituted by Fe or Al site in B2 FeAl. Detailed discussion on the thermodynamic and magnetic properties and electronic structure of these intermetallic compounds will be given.

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Characteristics of Musim Stream by Surveyed Sites Based on EPT-group of Aquatic Insects1a (수서곤충 EPT-group을 이용한 무심천의 조사지점별 특성)

  • Shin, Hyun-Seon;Mitamura, Osamu;Kim, Sook-Jung;Choi, Jun-Kil
    • Korean Journal of Environment and Ecology
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    • v.22 no.4
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    • pp.420-426
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    • 2008
  • This study compared and analyzed EPT ratio, EPT richness and EPT number(number of species and individuals) of a total of 9 selected sites individually using EPT-group of aquatic insects in Musim stream, also compared similarity ratio between the sampling sites using UPGMA(Unweighted Paired Group Method using Arithmetic average) based on analysis subsequent to species structure of aquatic insects, and compared and analyzed a functional feeding groups(FFGs) of aquatic insects based on stream order. As a result, the aquatic insect group of 2,269 individuals is classified as 71 species, 36 families, and 8 orders, and EPT ratio and EPT richness at sites 3(0.72 and 3.89, respectively) were found to be relatively higher than those at the other sites; by contrast, those at site 9(0.03 and 0.09, respectively) were relatively low. EPT number of species and individuals was found to be lowest at site 9. As a result of UPGMA analysis, the sampling sites in Musim stream were divided into three groups, including A1(site 1, 7, 8), A2(site 2, 3, 4, 5, 6) and B(site 9). According to the results of FFGs, there was not shown specific difference in the number of functional feeding groups by each sites; however, there appeared a little more shredders and scrappers in the upper and mid stream comparing to other sampling sites while collector-filterers and collector-gatherers were found to be dominant in number in the lower stream. According to this research result, the sampling sites in Musim stream were found to have an effect on not only EPT richness, EPT ratio, and EPT number but also FFGs structure.

Concentration and Gas-particle Partition of PCDDs/Fs and dl-PCBs in the Ambient Air of Ansan Area (안산지역 대기 중 다이옥신 및 dl-PCBs의 오염특성 조사)

  • Heo, Jong-Won;Kim, Dong-Gi;Song, Il-Seok;Lee, Gang-Woong
    • Journal of Korean Society for Atmospheric Environment
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    • v.26 no.5
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    • pp.517-532
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    • 2010
  • After establishment of Banwol industrial complex in 1987, Ansan city becomes the largest industrial sector development in Gyeonggi-do, Korea. As the population and industrial activity grow over this region, toxic air pollutants, particularly POPs (Persistent Organic Pollutants) from various emission sources have been major public concerns. Air samples for POPs monitoring were collected at the industrial sites ($A_2$), residential sites ($B_1$, $B_2$), commercial site (C), and rural/remote site (D) of the area of Ansan during 2008 with a prolonged industrial sampling site $A_1$ from 2001 to 2008. All samples were analysed for 2,3,7,8 substituted-polychlorinated dibenzo-p-dioxin and dibenzofurans (PCDD/Fs) and dioxin like polychlorinatd diphenyls (dl-PCBs). In site $A_1$, a steady decline of their concentrations from 2003 to 2008 was observed due to the reinforced emission guideline from waste incinerators. The average concentration of the PCDD/Fs and dl-PCBs ranged between 0.118 pg-TEQ/$m^3$ (rural/remote site D) and 0.532 pg-TEQ/$m^3$ (industrial area $A_2$). These level were generally consistent with previous studies in Gyeonggi-do, while higher than other places. Most of PCDD/Fs congener were partitioned into particle phase, whereas dl-PCBs were partitioned into gas phase. The logarithm of gas-particle partition coefficient $K_P$ of dl-PCBs and PCDD/Fs were well correlated with sub-cooled liquid vapor pressure $P_L$. The slope $m_T$ of log $K_P$ versus log $P_L$ for PCDD/Fs (-1.22) and dl-PCBs (-1.02) in industrial area ($A_2$) were high compared to other residential/commercial area. It suggests that this area was likely influenced by the direct emission source of PCDD/Fs and dl-PCBs. To simulate the partition of PCDD/Fs and dl-PCBs between gas and particle phase, Junge-Pankow model ($P_L$-base) and $K_{oa}$ model were applied. It was found that J-P model was more suitable than the $K_{oa}$ model in this study.

Ligand-Based Virtual Screening for inhibitors of PTP-1B with Antihyperglycemic properties

  • Kim, Heung-Jae;Yoo, Moo-Hi;Son, Mi-Won;Kim, Soon-Hoe
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.359.3-359.3
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    • 2002
  • Protein-tyrosine phosphatase 1 B(PTP-l B). which plays a key role in insulin signaling. is rising as a fascinating target for type 2 diabetes and obesity. Many scientists in structural biology solved the three dimensional X-ray Crystal structure of this type of enzyme, so we could easily get the active site structure of PTP-1 B or complex structure with ligand. Our virtual screening study for PTP-1B exactly based on these crystal strucutures from public database. (omitted)

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The changes of immune-related molecules within the ileal mucosa of piglets infected with porcine circovirus type 2

  • Shi, Fengyang;Li, Qiuming;Zou, Zhanming;Wang, Yang;Hou, Xiaolin;Zhang, Yonghong;Song, Qinye;Zhou, Shuanghai;Li, Huanrong
    • Journal of Veterinary Science
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    • v.21 no.5
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    • pp.78.1-78.15
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    • 2020
  • Background: Enteritis is one of the most frequently reported symptoms in piglets infected with porcine circovirus type 2 (PCV2), but the immunopathogenesis has not been reported. Objectives: This study examined the effect of a PCV2 infection on the intestinal mucosal immune function through morphological observations and immune-related molecular detection. Methods: Morphological changes within the ileum of piglets during a PCV2 infection were observed. The expression of the related-molecules was analyzed using a gene chip. The immunocyte subsets were analyzed by flow cytometry. The secretory immunoglobulin A (SIgA) content was analyzed by enzyme-linked immunosorbent assay. Results: The PCV2 infection caused ileal villus damage, intestinal epithelial cells exfoliation, and an increase in lymphocytes in the lamina propria at 21 days post-infection. Differentially expressed genes occurred in the defense response, inflammatory response, and the complement and coagulation cascade reactions. Most of them were downregulated significantly at the induction site and upregulated at the effector site. The genes associated with SIgA production were downregulated significantly at the induction site. In contrast, the expression of the Toll-like receptor-related genes was upregulated significantly at the effector site. The frequencies of dendritic cells, B cells, and CD8+T cells were upregulated at the 2 sites. The SIgA content decreased significantly in the ileal mucosa. Conclusions: PCV2 infections can cause damage to the ileum that is associated with changes in immune-related gene expression, immune-related cell subsets, and SIgA production. These findings elucidated the molecular changes in the ileum after a PCV2 infection from the perspective of intestinal mucosal immunity, which provides insights into a further study for PCV2-induced enteritis.