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Association of Polymorphisms in Stress-Related TNFα and NPY Genes with the Metabolic Syndrome in Han and Hui Ethnic Groups

  • Bu, De-Yun;Ji, Wen-Wu;Bai, Dan;Zhou, Jian;Li, Hai-Xia;Yang, Hui-Fang
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.14
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    • pp.5895-5900
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    • 2014
  • Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

Influence of Transgenic Corn on the In vitro Rumen Microbial Fermentation

  • Sung, Ha Guyn;Min, Dong Myung;Kim, Dong Kyun;Li, De Yun;Kim, Hyun Jin;Upadhaya, Santi Devi;Ha, J.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.12
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    • pp.1761-1768
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    • 2006
  • In this study, the comparative effects of transgenic corn (Mon 810 and Event 176) and isogenic corn (DK729) were investigated for their influence on in vitro rumen fermentation. This study consisted of three treatments with 0.25 g rice straw, 0.25 g of corn (Mon810/Event176/DK 729) mixed with 30 ml rumen fluid-basal medium in a serum bottle. They were prepared in oxygen free conditions and incubated at $39^{\circ}C$ in a shaking incubator. The influence of transgenic corn on the number of bacterial population, F. succinogenes (cellulolytic) and S. bovis (amylolytic), was quantified using RT-PCR. Fermentative parameters were measured at 0, 2, 4, 8, 12 and 24 h and substrate digestibility was measured at 12 and 24 h. No significant differences were observed in digestibility of dry matter, NDF, ADF at 12 and 24 h for both transgenic and isogenic form of corns (p>0.05) as well as in fermentative parameters. Fluid pH remained unaffected by hybrid trait and decreased with VFA accumulation as incubation time progressed. No influence of corn trait itself was seen on concentration of total VFA, acetic, propionic, butyric and valeric acids. There were no significant differences (p<0.05) in total gas production, composition of gas (methane and hydrogen) at all times of sampling, as well as in NH3-N production. Bacterial quantification using RT-PCR showed that the population number was not affected by transgenic corn. From this study it is concluded that transgenic corn (Mon810 and Event 176) had no adverse effects on rumen fermentation and digestibility compared to isogenic corn. However, regular monitoring of these transgenic feeds is needed by present day researchers to enable consumers with the option to select their preferred food source for animal or human consumption.

Production of ʟ-Theanine Using Escherichia coli Whole-Cell Overexpressing γ-Glutamylmethylamide Synthetase with Baker's Yeast

  • Yang, Soo-Yeon;Han, Yeong-Hoon;Park, Ye-Lim;Park, Jun-Young;No, So-young;Jeong, Daham;Park, Saerom;Park, Hyung Yeon;Kim, Wooseong;Seo, Seung-Oh;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.30 no.5
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    • pp.785-792
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    • 2020
  • ʟ-Theanine, found in green tea leaves has been shown to positively affect immunity and relaxation in humans. There have been many attempts to produce ʟ-theanine through enzymatic synthesis to overcome the limitations of traditional methods. Among the many genes coding for enzymes in the ʟ-theanine biosynthesis, glutamylmethylamide synthetase (GMAS) exhibits the greatest possibility of producing large amounts of production. Thus, GMAS from Methylovorus mays No. 9 was overexpressed in several strains including vectors with different copy numbers. BW25113(DE3) cells containing the pET24ma::gmas was selected for strains. The optimal temperature, pH, and metal ion concentration were 50℃, 7, and 5 mM MnCl2, respectively. Additionally, ATP was found to be an important factor for producing high concentration of ʟ-theanine so several strains were tested during the reaction for ATP regeneration. Baker's yeast was found to decrease the demand for ATP most effectively. Addition of potassium phosphate source was demonstrated by producing 4-fold higher ʟ-theanine. To enhance the conversion yield, GMAS was additionally overexpressed in the system. A maximum of 198 mM ʟ-theanine was produced with 16.5 mmol/l/h productivity. The whole-cell reaction involving GMAS has greatest potential for scale-up production of ʟ-theanine.

A Study on the Combination of the Existing Monitoring System with the Safety Management Network System (기구축 계측시스템의 안전관리 네트워크 시스템 연계 방안에 관한 연구)

  • Lee, Chin-Hyung;Park, Ki-Tae;You, Young-Jun;Joo, Bong-Chul;Hwang, Yoon-Koog;Bae, In-Hwan
    • Journal of Korean Society of societal Security
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    • v.2 no.3
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    • pp.47-53
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    • 2009
  • This study performed the construction of test bed for the combination of the health monitoring system In Yongjong grand bridge with the safety management network system in order to explore the feasibility of combining the existing monitoring system with the safety management network system. For this purpose, connection program which consists of DAQ sender server program and DAQ receiver server program has been developed. DAQ sender server program installed in the connection server, which is located on the DMZ zone of the existing monitoring system, plays the role of communicating with the monitoring system. On the other hand, DAQ receiver server program installed in the connection server, which is located on the integrated operation center of the safety management network system, plays the part of gathering real time and tim history data of the existing monitoring system and storing them in the integrated operation server through the communication with the DAQ sender. Test bed demonstrated the feasibility of combining the existing monitoring system with the safety management network system.

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State-Aware Re-configuration Model for Multi-Radio Wireless Mesh Networks

  • Zakaria, Omar M.;Hashim, Aisha-Hassan Abdalla;Hassan, Wan Haslina;Khalifa, Othman Omran;Azram, Mohammad;Goudarzi, Shidrokh;Jivanadham, Lalitha Bhavani;Zareei, Mahdi
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.1
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    • pp.146-170
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    • 2017
  • Joint channel assignment and routing is a well-known problem in multi-radio wireless mesh networks for which optimal configurations is required to optimize the overall throughput and fairness. However, other objectives need to be considered in order to provide a high quality service to network users when it deployed with high traffic dynamic. In this paper, we propose a re-configuration optimization model that optimizes the network throughput in addition to reducing the disruption to the mesh clients' traffic due to the re-configuration process. In this multi-objective optimization model, four objective functions are proposed to be minimized namely maximum link-channel utilization, network average contention, channel re-assignment cost, and re-routing cost. The latter two objectives focus on reducing the re-configuration overhead. This is to reduce the amount of disrupted traffic due to the channel switching and path re-routing resulted from applying the new configuration. In order to adapt to traffic dynamics in the network which might be caused by many factors i.e. users' mobility, a centralized heuristic re-configuration algorithm called State-Aware Joint Routing and Channel Assignment (SA-JRCA) is proposed in this research based on our re-configuration model. The proposed algorithm re-assigns channels to radios and re-configures flows' routes with aim of achieving a tradeoff between maximizing the network throughput and minimizing the re-configuration overhead. The ns-2 simulator is used as simulation tool and various metrics are evaluated. These metrics include channel-link utilization, channel re-assignment cost, re-routing cost, throughput, and delay. Simulation results show the good performance of SA-JRCA in term of packet delivery ratio, aggregated throughput and re-configuration overhead. It also shows higher stability to the traffic variation in comparison with other compared algorithms which suffer from performance degradation when high traffic dynamics is applied.

A Quantitative Method for the Assessment of Myocardial Function using the Polar Analysis of Tc-99m-MIBI Myocardial SPECT (Tc-99m-MIBI 심근 SPECT 극성지도 분석에 의한 심근 기능의 정량적 평가)

  • Kwark, Cheol-Eun;Lee, Dong-Soo;Yeo, Jung-Suk;Lee, Kyung-Han;Chung, June-Key;Lee, Myung-Chul;Seo, Joung-Don;Koh, Chang-Soon
    • The Korean Journal of Nuclear Medicine
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    • v.28 no.2
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    • pp.172-176
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    • 1994
  • As the Tc-99m-MIBI myocardial SPECT demonstrated wide application in the diagnosis of myocardial function, the quantitative and severity-dependent information is currently re quired. In this study, we proposed a computerized method for scoring the fixed defects in terms of extent-weighted severity and for identifying the reversibility in ischemic regions. At the first stage of this method, the transverse slices were reconstructed with 0.4 Nyquist freq. and order 5 Butterworth filter. From the oblique/sagittal slices, maximal count per pixel circumferential profiles were extracted for each sector, and then stress/redist. polar maps were normalized and plotted. For reversibility, the stress polar map was subtracted from the de-layed image and positive-valued pixels were categorized into three grades. The extent-weight-ed severity scores were calculated using the assigned grades and their number of pixels. This procedure was done automatically and the reversibility and severity scores were produced for each of the coronary territories (LAD, RCA, LCX) or any combination of these. Clinical ap-plication has shown that the changes In reversibility scores after PTCA were correlated linearly with the pre PTCA scores(r>0.8) in postinfarct cases as well as in angina, and severity scores of persistent defects in stress/rest SPECT study matched to the regional ejection fraction and visual analysis of regional wall motion of gated blood pool scan(r>0.6). We conclude that the computerized severity scoring method for the analysis of myocardial SPECT could be useful in the assessment of the myocardial ischemia and fixed defect.

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Herbicidal Activity of Thiocarbamate Herbicides and Its Effect on Cell Division and Elongation (Thiocarbamate계(系) 잡초제(雜草劑)의 살초력(殺草力)과 세포분열(細胞分裂) 및 신장(伸長)에 미치는 영향(影響))

  • Chun, J.C.;Lee, C.K.;Ma, S.Y.
    • Korean Journal of Weed Science
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    • v.15 no.1
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    • pp.46-53
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    • 1995
  • The herbicidal activity of thiocarbamate herbicides and its effect on cell division and elongation were determined. The herbicides studied were molinate(S-ethyl-N,N-hexamethylenethiocarbamate), dimepiperate [S-(${\alpha}$,${\alpha}$-di methylbenzyle)piperidine-1-carbothioate], esprocarb [S-benzyl-N-ethyl-N-(1,2-dimethylpropyl) thiocarbamate], and thiobencarb [S-(4-chlorobenzyl)-N, N-diethylthiocarbamate]. The herbicides applied at the rates ranged from $10^{-6}$ to $10^{-5}M$ did not affect germination and post-germination root growth of rice(Oryza sativa L.) and barnyardgrass [Echinochloa crus-galli(L.) P. Beauv.], but inhibited the post-germination shoot growth. There was no inhibition of gibberellin-induced ${\alpha}$-amylase biosynthesis in de-embryonated rice seeds by the herbicides at $10^{-5}M$, but about 50-60% inhibition occurred at $10^{-4}M$. When the herbicides were applied 7 days after seeding, the rates required to 50% growth inhibition of barnyardgrass were 146g, 91g, 96g, and 102g ai/10a for molinate, dimepiperate, esprocarb, and thiobencarb, respectively. No effect of the herbicides on cell division was found at $10^{-4}M$, but about 31 to 47% inhibition as compared with the untreated check was obtained by treatment of the herbicides at $10^{-3}M$. However, about 33 to 38% inhibition of cell elongation occurred at $10^{-5}M$ of the herbicides. They also inhibited IAA-induced cell elongation.

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Surgical Treatment of the Pseudoaneurysm of the Ascending Aorta Following the Repair of Congenital VSD -1 case report- (선천성 심실중격결손증 교정술 후 발생한 가성 상행대동맥류 수술 치험 -1례 보고-)

  • An, Byeong-Hui;Kim, Gwang-Hyu;Na, Guk-Ju;Kim, Sang-Hyeong
    • Journal of Chest Surgery
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    • v.29 no.5
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    • pp.564-568
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    • 1996
  • Pseudoaneurysm of the ascending aorta following cardiac surgery is very unusual and it is poten- tially fatal. We report here a fourteen year-old female patient with pseudoaneurysm of the ascending aorta following a repair of a congenital ventricular septal defect at other hospital 50 months ago. Although she had a mild superficial wound infection postoperatively, she enjoyed uneventful. life until she visited our hospital for a generalized weakness and exertional dyspnea which developed a month ago. Chest CT and echocardiogram showed partially calcified pseudoaneurysm of the ascending aorta. Two aortic defects were located on the anterolateral ascending aortic wall wkere it was suspected as a previous sites of aortic and cardioplegic cannulation. The internal wall of the pseudoaneurysm was covered with neoendothelium and intervened by septal tissue. Two defects on he aortic wall were oval in shape and about 1.5cm in the greatest diameter The defects were trimmed to make a one large de- fect and it was reconstructed with patch designed from 22mm collagen impregnated double velour Dacron graft. The postoperative course was uneventful.

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Development of Rustling Sound Generator Using Reciprocating Motion and Evaluation of Its Fabric Sound (왕복운동에 의한 직물마찰음발생장치의 개발 및 이를 이용한 직물소리 평가)

  • Kim Chun-Jeong;Cho Gil-Soo
    • Science of Emotion and Sensibility
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    • v.9 no.2
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    • pp.133-140
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    • 2006
  • In order to investigate the sensation of the fabric sound simulating the real wear-condition, the fabric sound simulator using reciprocating friction was developed. Fabric sounds from 5 specimen were generated by the fabric sound simulator and recorded using high performance microphone. Physical sound parameters of fabrics including level pressure of total sound (LPT), level range (${\Delta}L$), and frequency differences (${\Delta}f$) were calculated. For psychological evaluation, seven adjectives for sound (softness, loudness, sharpness, clearness, roughness, highness, and pleasantness) were used as the semantic differential scale. Fabric sounds by reciprocating friction of nylon taffeta and polyester leno had the highest value of LPT and evaluated as loud, sharp, rough, and unpleasant while polyester ultra suede and silk crepe de chine haying the lower LPT and ${\Delta}f$ were perceived as soft and quite. Comparing with fabric sound by one-way friction, fabric sound by reciprocation friction was perceived as more sharp, loud, and rough. LPT was also the most important factor affecting the sensation of the fabric sound by reciprocating friction.

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Salient Object Extraction from Video Sequences using Contrast Map and Motion Information (대비 지도와 움직임 정보를 이용한 동영상으로부터 중요 객체 추출)

  • Kwak, Soo-Yeong;Ko, Byoung-Chul;Byun, Hye-Ran
    • Journal of KIISE:Software and Applications
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    • v.32 no.11
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    • pp.1121-1135
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    • 2005
  • This paper proposes a moving object extraction method using the contrast map and salient points. In order to make the contrast map, we generate three-feature maps such as luminance map, color map and directional map and extract salient points from an image. By using these features, we can decide the Attention Window(AW) location easily The purpose of the AW is to remove the useless regions in the image such as background as well as to reduce the amount of image processing. To create the exact location and flexible size of the AW, we use motion feature instead of pre-assumptions or heuristic parameters. After determining of the AW, we find the difference of edge to inner area from the AW. Then, we can extract horizontal candidate region and vortical candidate region. After finding both horizontal and vertical candidates, intersection regions through logical AND operation are further processed by morphological operations. The proposed algorithm has been applied to many video sequences which have static background like surveillance type of video sequences. The moving object was quite well segmented with accurate boundaries.