• Annals of Hepato-Biliary-Pancreatic Surgery
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• v.27 no.2
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• pp.166-171
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• 2023

Backgrounds/Aims: Routine execution of intraoperative cholangiography (IOC) in laparoscopic cholecystectomy (LC) is considered a good practice to help early identification of biliary duct injuries (BDIs) or common bile duct (CBD) stones. This study aimed to determine the impact of IOC during LC. Methods: This is a retrospective, monocentric study, including patients with a LC performed from January 2020 to December 2021. Results: Of 303 patients, 215 (71.0%) were in the IOC group and 88 (29.0%) in the no-IOC group. IOC was incomplete or unclear in 10.7% of patients, with a failure rate of 14.7%. Operating time was 15 minutes longer in the IOC group (p = 0.01), and postoperative complications were higher (5.1% vs. 0.0%, p = 0.03). There were three BDIs (0.99%), all included in the IOC group; only one was diagnosed intraoperatively, and the other two were identified during the postoperative course. Regarding identifying CBD stones, IOC showed a sensitivity of 77%, a specificity of 98%, an accuracy of 97.2%, a positive predictive value of 63% and a negative predictive value of 99%. Conclusions: Systematic IOC has shown no specific benefits and prolonged operative duration. IOC should be performed on selected patients or in situations of uncertainty on the anatomy.

• Coupled systems mechanics
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• v.13 no.4
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• pp.311-335
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• 2024

This work aims to show a model to estimate the minimum cost (Thickness and area of steel in X and Y directions) for design a circular isolated footing with eccentric column that considers that the surface in contact with the ground works partially under compression. The formulation is shown by integration to find the moments, the bending shears and the punching shear using the pressure volume under the footing. Some researchers show the minimum cost design for circular isolated footings for an eccentric column assuming that the contact area works completely in compression, others consider the contact surface with the ground working partially in compression for a column in the center of the base. Three numerical examples are developed to obtain the complete design, which are: Example 1 for a column in the center of the base,Example 2 for a column at a distance of 1.50 m from the center of the base in the X direction, Example 3 for a column at a distance of 1.50 m from the center of the base in both directions. Also, a comparison of the new model against the model proposed by other authors is presented. The comparison shows that the new model generates a great saving of up to 43.74% for minimum area and 48.44% for minimum cost design in a column located in the center of the base, and when the column is located at a distance of radius/2 starting from the center of the base in the X direction generates great savings of up to 45.24% for minimum area and 31.80% for minimum cost design. Therefore, it is advisable to use the model presented in this study.

• Archives of Plastic Surgery
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• v.45 no.2
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• pp.165-170
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• 2018

Background To present the outcomes of the tarsal switch procedure using an anterior approach to correct severe ptosis with poor levator muscle function (<4 mm) with absent or poor Bell's phenomenon. Methods This retrospective case series included 11 patients with severe neurogenic or acquired myogenic palpebral ptosis. All patients underwent the tarsal switch procedure through an anterior approach from 2012 to 2015. Margin reflex distance (MRD1 and MRD2) and the palpebral fissure were evaluated preoperatively and postoperatively. Data were compared using the Wilcoxon signed-rank test. P-values <0.05 were considered to indicate statistical significance. Results Surgery was performed on 18 eyelids (11 patients). The median age at surgery was 57 years (range, 29-86 years). Four patients had unilateral ptosis and seven had bilateral ptosis. Nine patients had myogenic ptosis and two had neurogenic ptosis. Postoperatively, the chin-up position improved in all patients. The MRD1 increased statistically significantly, from 0 mm preoperatively to 1.0 mm postoperatively (P=0.001). The MRD2 decreased statistically significantly, from 4.5 mm preoperatively to 3.0 mm postoperatively (P=0.001). The palpebral fissure did not change (4.0 mm preoperatively to 4.0 mm postoperatively) (P=0.13). Conclusions The tarsal switch procedure through an anterior approach is an effective alternative for correcting severe ptosis, especially neurogenic or acquired myogenic ptosis. This procedure can be performed with minimal risk of ocular surface exposure and provides stable outcomes.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.21 no.2
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• pp.118-126
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• 2018

Purpose: Classical criteria for diagnosis of autoimmune hepatitis (AIH) are intended as research tool and are difficult to apply at patient's bedside. We aimed to study the accuracy of simplified criteria and the concordance with the expert diagnosis based on the original criteria. Methods: A cohort of children under study for liver disorder was selected through consecutive sampling to obtain the prevalence of AIH within the group of differential diagnoses. AIH was defined, based on classical criteria, through committee review of medical reports. Validity indicators of the simplified criteria were obtained in an intention to diagnose approach. Optimal cut-off and the area under the receiver operating characteristic (ROC) curve were calculated. Results: Out of 212 cases reviewed, 47.2% were AIH. For the optimal cut-off (6 points), the simplified criteria showed a sensitivity of 72.0% and a specificity of 96.4%, with a 94.7% positive and a 79.4% negative predictive value. The area under the ROC curve was 94.3%. There was a good agreement in the pre-treatment concordance between the classical and the simplified criteria (kappa index, 0.775). Conclusion: Simplified criteria provide a moderate sensitivity for the diagnosis of AIH, but may help in indicating treatment in cases under suspicion with 6 or more points.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.20-24
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• 2006

Plasmid DNA (pDNA) is a product of interest for many biopharmaceutical companies and research laboratories, because of increase in the number of gene therapy protocols that use nonviral vectors. This work was undertaken to study the effect of antibiotic and dissolved oxygen concentration (DOC) on the production of a ColE 1-type plasmid (pVAX1-LacZ) hosted in Escherichia coli $DH5\alpha$ and cultured in a batch fermentor with 0.751 of Terrific Broth. A decrease in the DOC from $60\%\;to\;5\%$ was shown to increase the specific pDNA concentration approximately 1.5-fold, due to the downregulation of growth. Additionally, this increase in the pDNA concentration led to a 2.2-fold increase in the purity of cell lysates obtained after cell lysis. However, the use of higher DOC led to 2.8-fold higher volumetric productivity as a consequence of a faster growth rate, reducing the fermentation time from 24 to 8 h. Interestingly, the specific pDNA concentration, and pDNA productivity and purity were always higher $(10-15\%)$ in the absence of antibiotic. Overall, the data indicate that nonselective conditions can be used without compromising yield, productivity, and purity of pDNA.

• Journal of Veterinary Science
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• v.22 no.6
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• pp.81.1-81.9
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• 2021

Background: Suid gammaherpesvirus 3, 4, and 5 (porcine lymphotropic herpesvirus - PLHV-1, -2, and -3) are viruses that infect domestic and feral pigs. Objectives: This study examined the presence of PLHV DNA in biological samples from free-living wild boars circulating in a Brazilian geographical region with a high density of commercial domestic pigs. Methods: Lung samples of 50 free-living wild boars were collected by exotic wildlife controller agents between 2017 and 2019 in the state of Paraná, southern Brazil. Lung and spleen fragments were obtained from six fetuses collected by hysterectomy post mortem from a pregnant sow. A polymerase chain reaction (PCR) assay using consensus primers (pan-herpesviruses) was performed to detect PLHV DNA. The samples showing positive results for PLHV DNA were submitted to single-round PCR assays with the specific primers for identifying PLHV-1 (213-S/215-As), PLHV-2 (208-S/212-As), and PLHV-3 (886s/886As). The specificity of the species-specific PCR products was assessed by nucleotide sequencing of the amplicons. Results: Forty-eight (96%) of the 50 lung samples analyzed were positive for PLHV by PCR using pan-herpesvirus primers. In 33 (68.75%) of the positive samples, at least two PLHV species were identified simultaneously. The DNA of PLHV-1, -2, and -3 was found in free-living wild boars of all ages, but not in the fetuses, even though they were from a sow that tested positive for all three viruses. Conclusion: These viruses are endemic to the population of feral pigs in the Brazilian region evaluated, as well as in domesticated pigs.

• Bulletin of the Korean Mathematical Society
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• v.50 no.2
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• pp.675-685
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• 2013

We prove, under some general assumptions, that a generator of any uniformly bounded Nemytskij operator, mapping a subset of space of functions of bounded variation in the sense of Wiener-Young into another space of this type, must be an affine function with respect to the second variable.

• Honam Mathematical Journal
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• v.31 no.2
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• pp.167-176
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• 2009

The aim of this paper is to obtain three interesting results for reducibility of Kamp$\'{e}$ de $\'{e}$riet function. The results are derived with the help of contiguous Gauss's second summation formulas obtained earlier by Lavoie et al. The results obtained by Bailey, Rathie and Nagar follow special cases of our main findings.

• Journal of the Korean Mathematical Society
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• v.45 no.2
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• pp.527-535
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• 2008

Sufficient conditions are given to assert that between any two Banach spaces over $\mathbb{K}$ Fredholm mappings share exactly N values in a specific open ball. The proof of the result is constructive and is based upon continuation methods.

• The Korean Journal of Zoology
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• v.15 no.2
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• pp.57-69
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• 1972

According to the brachyuran specimens collected from the coastal seas of Korea during the period from 1969 to 1970, the following ten species were described briefly as hitherto unrecorded species from Korea: Family Calapidae: 1. Mursia curtispina trispinosa(Parisi); Family Corystidae: 2. Jonas distincta (de Haan); Family Portunidae: 3. Portunus (Monomia), argentatus (White); Family Xanthidae: 4. Neoliomer a insularis (White), 5. Acutumnus asper (Ruppell); Family Majidae: 6. Pleistacanta sanctijohannis Miers 7. Huenia proteus de Haan, 8. Scyra compressipes Stimpson, 9. Maja spinigera de Haan, 10. Entomonyx spino년 (Miers)