• 한국미생물·생명공학회지
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• 제13권2호
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• pp.157-162
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• 1985

Bacillus sphaericus 1593 K-5 균주의 Culex pallens 모기유충에 대한 LC$_{50}$(cells/$m\ell$)은 2.6$\times$$10^2$이었다. B. sphericus 1593 K-5 Spo-D 1216 균주는 Culex pallens 모기유충에 대해 독성을 나타내지 않았다. B. sphaericus 1593 K-5 균주는 포자가 형성되어 가면서 독성이 증가함을 보였다. B. sphaericus 1593 K-5 균주의 세포질을 Sep-hadex G-100 컬럼상에서 gel permeation 크로마토그래피를 행하였을 때 3개의 단백질 분획을 보였으며 이 중 한 분획이 활성분획있다. 이 활성 분획을 DEAE-Sepha dex A-50 컬럼상에서 ion-exchange 크로마토그래피를 행하였을 때 4개의 단백질 분획을 보였으며 이중 한 분획이 활성분획이었다. B. sphericus 1593 K-5 Spo-D1216균주의 세포질은 Sephadex G-100 컬럼상에서 gel permeation 크로마토그래피를 행하였을 때 2개의 단백질 분획을 보였으며 이 분획 모두가 활성이 없음이 생체실험 결과 밝혀졌다.다.

• 산업식품공학
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• 제21권2호
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• pp.103-109
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• 2017

DEAE sephacel anion chromatography 및 SP sepharose cation chromatography에 의해 Streptomyces violaceoruber 유래 alginate lyase의 정제를 수행하여 비활성 14.59 units/mL 정제배율 40.64배를 나타내었다. Tricine SDS-PAGE에 의한 단일밴드를 확인하였고, 분자량은 23.3 kDa으로 결정되었다. 정제효소에 의해 sodium alginate를 가수분해하여 1차 activated carbon column chromatography와 2차 bio gel P-2 gel filtration에 의해 당가수분해물을 분리 회수하여 TLC와 FACE를 통해 중합도를 확인하고 Timell's method에 의해 hetero type M/G-oligosaccharide 중합도 6, 8로 결정되었다. B. animalis, B. bifidum, B. breve, B. infantis, B. longum와 L. acidophilus, L. casei, L. reuteri에 생육활성에 대한 중합도 6, 8의 영향을 검토하기 위하여 modified-MRS media에 탄소원으로 중합도 6, 8를 대체하여 생육활성을 비교한 결과 B. longum에서는 D.P. 6 M/G-oligosaccharide를 탄소원으로 대체한 경우 표준 MRS배지와 비교하여 4.25배, D.P. 8에서 6.44배의 상대활성을 나타내어 가장 우수한 생육활성을 나타내었으며, B. bifidum의 경우에서도 D.P. 6에서 3.27배, D.P. 8에서 5.4배의 상대활성을 나타내었다. 이외에도 B. animalis, B. breve그리고 L. casei에 있어서도 D.P. 8의 경우 3배의 상대활성을 나타내었으나, L. reuteri에 대한 D.P. 8의 경우에서는 표준 MRS media와 비교하여 0.29배로 감소하였다. 결과적으로 D.P. 8의 올리고당이 D.P. 6의 올리고당보다 생육활성에 크게 기여하는 것으로 나타났다.

• 미생물학회지
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• 제30권4호
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• pp.291-298
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• 1992

비변성 폴리아크릴 아미드 겔에서 Streptomyces coelicolor 의 catalase 활성 염색을 실시하여 여러개의 catalase 활성 띠를 관찰하였으며, 그 유형이 성장기에 따라 달라짐을 알았다. 포자와 정체 성장기에는 정체 성장기에만 특이적인Cat1(760kD) 이 관찰되었고, Cat2(300kD) 를 제외한 모든 활성 띠들이 나타났다. 중기 대수 성장기에는 Cat2 와 Cat 3-2, Cat3-2(140kD) 등 2개의 catalase 띠가 나타나며, 후기 대수 성장기에는 Cat3-1(170 kD), Cat3-2, Cat3-3(130kD). Cat4(70kD)등의 활성 띠가 나타났다. NTG 처리로 돌연변이화된 S. coelicolor 의 포자를 Bennet 평판 배지네서 배양한 후 과산화수소 거품 test 를 실시하여, 약 5000 여개의 콜로니 중 거품형성 속도나 양이 감소한 콜로니를 12개 얻었다. 야생형에 견주어 대부분 catalase 활성이 감소하였으며, 대수 성장기와 정체 성장기에서 모두 감소하였다. 거품형헝을 하지 않는 모든 돌연변이에서 Cat3-2 띠의 강도라 현저히 약해저 있음으로 보아 Cat3-2가 주된 catalase 인 것으로 추정된다. 대수 성장기에서 수확한 S. coelicolor 세포 추출액에서 Sepharose CL-4B, DEAE Sepharose CL-6B, Phenyl Sepharose CL-4B, Hydroxylapatite 크로마토그래피등 4단계의 크로마토그래피를 수행하여 Cat3-2 를 정제하였다. 비변성 폴리아크릴마드 겔 전기 영동을 수행한 결과 Cat3-2 는 67 kD 의 동일한 subunit 을 2개 가지고 있는 효소로 추정된다.

• 한국의학물리학회지:의학물리
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• 제32권4호
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• pp.99-106
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• 2021

Purpose: In this study, we aimed to manufacture a patient-specific gel phantom combining three-dimensional (3D) printing and polymer gel and evaluate the radiation dose and dose profile using gel dosimetry. Methods: The patient-specific head phantom was manufactured based on the patient's computed tomography (CT) scan data to create an anatomically replicated phantom; this was then produced using a ColorJet 3D printer. A 3D polymer gel dosimeter called RTgel-100 is contained inside the 3D printing head phantom, and irradiation was performed using a 6 MV LINAC (Varian Clinac) X-ray beam, a linear accelerator for treatment. The irradiated phantom was scanned using magnetic resonance imaging (Siemens) with a magnetic field of 3 Tesla (3T) of the Korea Institute of Nuclear Medicine, and then compared the irradiated head phantom with the dose calculated by the patient's treatment planning system (TPS). Results: The comparison between the Hounsfield unit (HU) values of the CT image of the patient and those of the phantom revealed that they were almost similar. The electron density value of the patient's bone and brain was 996±167 HU and 58±15 HU, respectively, and that of the head phantom bone and brain material was 986±25 HU and 45±17 HU, respectively. The comparison of the data of TPS and 3D gel revealed that the difference in gamma index was 2%/2 mm and the passing rate was within 95%. Conclusions: 3D printing allows us to manufacture variable density phantoms for patient-specific dosimetric quality assurance (DQA), develop a customized body phantom of the patient in the future, and perform a patient-specific dosimetry with film, ion chamber, gel, and so on.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.94.2-94.2
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• 2007

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• Current Research on Agriculture and Life Sciences
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• 제31권1호
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• pp.30-37
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• 2013

Bacillus subtilis and Bacillus amyloliquefaciens are closely related species that share a similar genomic background, and are both known to secrete large amounts of proteins directly into a medium. The extracellular proteomes of two strains of Bacillus subtilis and two strains of Bacillus amyloliquefaciens were compared by 2-D gel electrophoresis during the late exponential growth phase. The relative abundance of some minor protein spots varied among the four strains of Bacillus. Over 123 spots of extracellular proteins were visualized on the gel for B. subtilis CH 97, 68 spots for B. subtilis 3-5, 230 spots for B. amyloliquefaciens CH 51, and 60 spotsfor B. amyloliquefaciens 86-1. 2D gel electrophoresis images of the four Bacillus strains showed significantly different protein profiles. Consistent with the 2D gel electrophoretic analysis, most of the B. subtilis proteins differed from the proteases secreted by the B. amyloliquefaciensstrains. Among the proteins identified from B. subtilis, approximately 50% were cytoplasmic and 30% were canonically extracellular proteins. The secreted protein profiles for B. subtilis CH 97 and B. subtilis 3-5 were quite different, as were the profiles for B. amyloliquefaciens CH 51 and 86-1. The four proteomes also differed in the major protein composition. The B. subtilis CH 97 and B. amyloliquefaciens CH 51 proteomes both contained large amounts of secreted hydrolytic enzymes. Among the four strains, B. subtilis 3-5 secreted the least number of proteins. Therefore, even closely related bacteria in terms of genomic sequences can still have significant differences in their physiology and proteome layout.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.432-436
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• 2002

A proteomic map of Hanwoo loin was obtained using 2-D SDS-PAGE and mass spectrometric analysis: 27 bovine proteins plus 2 proteins having similarities to other mammal proteins out of 52 proteins analyzed. The identified proteins consisted of 50 % basic house keeping proteins involved in metabolism, 30% muscle proteins, and other miscellaneous proteins. Many proteins on the 2-D gel with different molecular weights and isoelectric points were identified as same proteins due to posttranslational modification. As many of the identified house keeping proteins showed the high sequence similarities to other mammal equivalent proteins, searching the mammal databases could confirm the annotation. The preliminary identification of the proteome in bovine loin tissue could reveal the functions of proteins at over 50 % of chance with high fidelities. Using the established loin proteome map, proteomic difference between 1 yr and 2 yr Hanwoo loin tissues were compared on 2D gel. Regardless of the difficulty normalizing protein concentrations and sample-to-sample variations, three unidentified proteins and myoglobin were selected as up-regulated proteins during the fat deposition period. This study contributes to a move thorough and holistic understanding of beef meat, helping to build the basis for future identification of new markers for good quality meat.

• KSBB Journal
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• 제14권5호
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• pp.578-584
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• 1999

겨우살이가 가지고 있는 lectin의 약리작용의 가능성에 대한 연구의 일환으로, lectin을 분리 . 정제하였으며, 분자량, subunit수, 탄수화물의 조성, 단백질의 아미노산 조성, 적혈구 응집력, 탄수화물 특이성, pH 및 열 안정성등의 생화학적 특성을 연구하였다. Lectin의 분리는 0.15 M NaCl에 의한 추출, $(NH_4)_2SO_4$ 침전, sepharose 4B affinity chromatography 및 sephadex G-150 gel filtration에 의해 11.64배 정제에 0.14%의 수율을 획득하였으며, HPLC와 전기영동을 이용하여 순도를 검정하였다. Gel filtration에 의해 분석된 lectin의 분자량은 124,000 Da이였고, SDS-PAGE에 의해 분자량이 30,000과 32,000 Da인 2개의 band로 나타나서 각각 2개의 subunit를 갖는 tetramer로 확인되었다. 겨우살이 lectin의 탄수화물은 glucose, fructose, arabinose 및 xylose를 함유하고 있다. 또한 phenlylalanine, lysine, tyrosine, aspartic acid가 비교적 풍부하였고 methionine, alanine, arginine은 비교적 적었다. Lectin은 적혈구에 종 특이성을 나타내는데 겨우살이의 혈구응집반응에서는 사람혈액형간의 차이는 없었으며, 쥐에서 가장 높았고 소에서 가장 낮았으므로 동물 종간에 대한 특이성은 뚜렸하였다. Lectin은 당 특이성을 가지는데, D-galactose, D-mannose, D-lactose 및 D-raffinose에 대해 특이성을 나타내었다. 겨우살이 lectin은 pH4~7에서 안정하였으며, 5$0^{\circ}C$까지는 10~30분 열처리해도 활성이 유지되는 열 안정성 단백질이었다.

• BMB Reports
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• 제39권2호
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• pp.216-222
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• 2006

In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT(dithiothreitol) and 0.2% carrier ampholytes; (b) 5M urea, 2M thiourea, 2% CHAPS, 2% SB 3-10 (N-decyl-N, N-dimethyl-3-ammonio-1-propanesulfonate), 40mM Tris base, 65 mM DTT and 0.2% carrier ampholytes or (c) 7M urea, 2M thiourea, 4% CHAPS, 65 mM DTT and 0.2% carrier ampholytes. The optimal protocol for isoelectric focusing (IEF) was accumulated voltage of 16,500 Vh and 0.6% DTT in the rehydration solution. In the experiments conducted for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), best results were obtained with a doubled concentration (50 mM Tris, 384 mM glycine, 0.2% SDS) of the SDS electrophoresis buffer in the cathodic reservoir as compared to the concentration in the anodic reservoir (25 mM Tris, 192 mM glycine, 0.1% SDS). Among the five protocols tested for gel storing, success was attained when the gels were stored in plastic bags with 50% glycerol. This is the first report describing the successful solubilization and 2D-electrophoresis of proteins from human lymph node tissue and a 2-D gel storage protocol for easy gel handling before mass spectrometry (MS) analysis.

• Corrosion Science and Technology
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• 제22권6호
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• pp.447-456
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• 2023

In this work, we performed a comparative study examining two coatings on Ti Gr.1 for use in fuel cell bipolar plates. The coatings consisted of carbon black as the conductor along with acrylic polymer and Ti Sol-Gel binder as the binder. Ti Sol-Gel that had precipitated as TiO₂ in areas impregnated between carbon black gaps, thereby acting as a binder for carbon black and serving as a polymer coating. Neither of the coatings peeled off during the 90° bend test to check formability. The contact resistance of the TiO₂ coating was found to be lower than that of the polymer binder coating. Moreover, due to coating shrinkage (denser) that occurred during the heat treatment process, the TiO₂ binder coating showed almost the same level of corrosion resistance, as measured by potentiostatic and EIS tests, despite being thinner than the polymer coating. However, both the polymer binder coating and the TiO₂ binder coating had many pores and irregularities internally (around 10 ~ 100 nm) and on the surface (0.1 ~ 2 ㎛). We considered that these pores and irregularities contributed to the lower corrosion resistance.