• KSBB Journal
• /
• v.16 no.2
• /
• pp.146-152
• /
• 2001

To avoid the intrinsic problem of aggregation associated with the traditional solution-phase refolding process, we propose a solid-phase refolding method integrated with expanded bed adsorption chromatography. The model protein used was a fusion protein of recombinant human growth hormone and a glutathione S transferase fragment. It was demonstrated that the EBA-mediated refolding technique could simultaneously remove cellular debris and directly renature the fusion protein inclusion bodies in the cell homogenate with much higher yields and less agregation. To demonstrate the applicability of the method, we successfully tested the three representative types of starting materials, i. e., rhGH monomer, washed inclusion bodies, and the E. coli homogenate. This direct and simplified refolding process could also reduce the number of renaturation steps required and allow refolding at a higher concentration, at approximately 2 mg fusion protein per ml of resin. To the best of our knowledge, it is the first approach that has combined the solid-phase refolding method with expanded bed chromatography.

• Korean Journal of Chemical Engineering
• /
• v.35 no.11
• /
• pp.2185-2190
• /
• 2018

Methane activation through oxychlorination is in the spotlight due to the relatively mild reaction conditions at atmospheric pressure and in the temperature range of $450-550^{\circ}C$. Although $CeO_2$ is known to exhibit good activity for methane oxychlorination, significant amounts of by-products such as $CO_2$, CO and carbon deposits are produced during the reaction over $CeO_2$. We investigated the effect of iron in $FeO_x/CeO_2$ catalysts on methane oxychlorination. $FeO_x/CeO_2$ with 3 wt% iron shows the maximum yield at $510^{\circ}C$ with 23% conversion of methane and 65% selectivity of chloromethane. XRD and $H_2$ TPR results indicate that iron-cerium solid solution was formed, resulting in the production of more easily reduced cerium oxide and the suppression of catalysts sintering during the reaction. Furthermore, the selectivity of by-products decreased more significantly over $FeO_x/CeO_2$ than cerium oxide, which can be attributed to the facilitation of HCl oxidation arising from the enhanced reducibility of the former sample.

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.13 no.2
• /
• pp.27-34
• /
• 2005

This study is performed to analyze the turbulence distribution of Fev. & Mar. in 2000 by the analysis of the parameters related with flight data of FDR(Flight Data Recorder). In the analysis, we selected the Solid State Flight Data Recorder(SSFDR) & Universal Flight Data Recorder(UFDR) flight data of the exact same aircraft(capacity 120 persons). Through this study, we verified that turbulence is concerned with configuration of the ground and flight situation of aircraft.

• Journal of Fisheries and Marine Sciences Education
• /
• v.28 no.2
• /
• pp.401-406
• /
• 2016

Elevator is very important transportation facility of modern society. But there have been frequent elevator accidents recently. In the view of safety management of elevator, the corresponding study must be done since accidents related elevator include not only actual accident but also possible accident in order to protect user from danger. A 3D design was developed using SolidWorks and behavior analysis using FEM Code.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.3
• /
• pp.153-157
• /
• 2001

Protoplasts were isolated from hypocotyls, cotyledons, and young leaves of Chinese cabbage grown under in vitro environmental condition. An enzyme mixture of 1% Cellulysin and 0.5% Macerozyme in combination with 0.4 M mannitol was most effective condition for protoplast isolation. The highest yield of protoplasts, 7.6$\times$10$^{5}$ protoplast/g of fresh weight, was obtained from the treatment of leaves for 12~16 hours at 27~28$^{\circ}C$ with shaking at 30 rpm. The most suitable medium for an initial cell division was K8p basal medium supplemented with 5 mg/L 2,4-D and 2 mg/L kinetin. Within 7~10 days, protoplasts derived from hypocotyl and cotyledon tissues formed cell colonies. When the cells were grown at the size of 8~10 cells, they were embedded into semi-solid medium containing 0.2% agarose. Calli derived from protoplast culture were transferred to the 100 different types of plant regeneration media, but no completely regenerated plants from inbred lines of Chinese cabbage used for this study wore obtained, though frequent rooting took place in several media tested.

• Applied Chemistry for Engineering
• /
• v.31 no.4
• /
• pp.383-389
• /
• 2020

Mesoporous silica solid catalysts modified with sulfonic acid were prepared for cationic ring-opening polymerization of octamethylcyclotetrasiloxane (D₄). Two sets of MCM-41 (1.7 and 2.8 nm) and SBA-15 (8.1 and 15.9 nm) with different pore sizes were used as catalyst supports. The surface of silica materials was modified with (3-mercaptopropyl)trimethoxysilane by silylation reaction and oxidized to sulfonic acid. The structures of the prepared catalysts were examined by X-ray diffraction and nitrogen adsorption-desorption. The pore size, specific surface area, and pore volume of the modified solid catalysts decreased slightly. In addition, the modification of the sulfonic acid on the silica surface was confirmed by using infrared spectroscopy and nuclear magnetic resonance spectroscopy. To observe the effect of the particle size on the catalytic activity, it was observed with a scanning electron microscope. The catalysts were used to synthesize PDMS through a ring-opening polymerization of D₄, and the conversion and polymerization rate of the polymerization reaction depended on the pore size, specific surface area, particle size, and particle agglomeration of the catalysts. In order for the polymerization rate, the catalyst prepared with SBA-15 of 8.1 nm pore size had the fastest reaction rate and showed the best catalytic activity.

• Geomechanics and Engineering
• /
• v.29 no.4
• /
• pp.471-486
• /
• 2022

The effect of segmental joints is one of main importance for the segmental lining design when tunnels are excavated by a mechanized process. In this paper, segmental tunnel linings are analyzed by two numerical methods, namely the Beam-Spring Method (BSM) and the Solid-Interface Method (SIM). For this purpose, the Tehran Subway Line 6 Tunnel is considered to be the reference case. Comprehensive 2D numerical simulations are performed considering the soil's calibrated plastic hardening model (PH). Also, an advanced 3D numerical model was used to obtain the stress relaxation value. The SIM numerical model is conducted to calculate the average rotational stiffness of the longitudinal joints considering the joints bending moment distribution and joints openings. Then, based on the BSM, a sensitivity analysis was performed to investigate the influence of the ground rigidity, depth to diameter ratios, slippage between the segment and ground, segment thickness, number of segments and pattern of joints. The findings indicate that when the longitudinal joints are flexible, the soil-segment interaction effect is significant. The joint rotational stiffness effect becomes remarkable with increasing the segment thickness, segment number, and tunnel depth. The pattern of longitudinal joints, in addition to the joint stiffness ratio and number of segments, also depends on the placement of longitudinal joints of the key segment in the tunnel crown (similar to patterns B and B').

• 한국정보디스플레이학회:학술대회논문집
• /
• 2004.08a
• /
• pp.640-643
• /
• 2004

We have synthesized SrS:Eu red phosphor by solid state method and investigated to adopt a red phosphor for LEDs. The SrS:Eu phosphor shows broad emission band at 600nm region due to f-d energy transfer of $Eu^{2{\cdot}}$. Our results show that the SrS:Eu red phosphor exhibits the better luminescence efficiency than that of the industrially available product SrS:Eu phosphor.

• Journal of the Korean Society of Industry Convergence
• /
• v.3 no.2
• /
• pp.107-114
• /
• 2000

Recently the studies on the vibration and the noise of a helical gear transmission have been focused on the many researchers. The manufacturing error and the deformation of the tooth profile, which generates the vibration and the noise of the gear transmission, are main factors. The major purpose of this study is to develop an optimal design program for reducing the vibration and the noise of the helical gear. To obtain the these results, we restrain the helical gear from the deformation of the tooth profile and increase the contact ratio within the optimal design program. Furthermore we made the three-dimensional solid modeling of the helical gear from the AutoCAD and the Pro/Engineer. This model will be available to generate the finite element model and the NC code.

• Journal of Korean Society for Atmospheric Environment
• /
• v.25 no.4
• /
• pp.255-263
• /
• 2009

The antifungal activity of silver, copper, and titania nanoparticles against fungal spores was investigated. Cladosporium cladosporioides spores were aerosolized and sampled on a solid agar plate using an Anderson impactor. The solid agar plate contained different concentration of nanoparticles ranging from 0 to $500{\mu}g/mL$. Silver and copper nanoparticles were shown to be an effective antifungal agent, while titania nanoparticles were not. Antifungal activity of these effective nanoparticles appeared at $300{\mu}g/mL$ concentration.