• International Journal of Oncology
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• v.54 no.2
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• pp.753-763
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• 2019

The mutation of isocitrate dehydrogenase (IDH)1 (R132H) and IDH2 (R172K) and the induction of hypoxia in various solid tumors results in alterations in metabolic profiles, including the production of the d- or l-forms of 2-hydroxyglutarate (2HG) from α-ketoglutarate in aerobic metabolism in the tricarboxylic acid (TCA) cycle. However, it is unclear whether the oncometabolite d-2HG increases angiogenesis in endothelial cells. Therefore, in this study, we analyzed the levels of various metabolites, including d-2HG, under hypoxic conditions and in IDH2R172K mutant breast cancer cells by mass spectrometry. We then further evaluated the effects of this metabolite on angiogenesis in breast cancer cells. The results revealed that treatment with d-2HG increased the levels of secreted vascular endothelial growth factor (VEGF) in cancer cells and enhanced endothelial cell proliferation in a concentration-dependent manner. Wound healing and cell migration (examined by Transwell assay) were significantly increased by d-2HG to a level similar to that induced by VEGF. Tube formation was significantly stimulated by d-2HG, and chick chorioallantoic membrane angiogenesis was also enhanced by d-2HG. d-2HG activated VEGF receptor (VEGFR)2 and VEGFR2 downstream signaling, extracellular signal-regulated kinase 1/2, focal adhesion kinase, AKT and matrix metalloproteinase (MMP)2. Taken together, the findings of this study suggested that d-2HG induced angiogenic activity via VEGFR2 signaling and increased MMP2 activity.

• Archives of Pharmacal Research
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• v.21 no.2
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• pp.217-222
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• 1998

A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-$\beta$-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs=1.83) on $5{\mu}m$ octadecylsilan column using 35% acetonitrile in 0.05M ammonium acetate buffer (pH=6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N=3).

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.11
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• pp.2228-2237
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• 1994

This paper describes the development of a Ku-band ($12.25GHz\sim12.75GHz$) SSPA intended as a replacement for TWTAs used in communication satelite transponder. The power stage of the amplifier consists of tow intrmally matched 8W FET divices combined using the branch-line coupler. To operate this stage, the drive stage has been designed with intermally matched 2W, 4W, 8W FET and two medium power FETs. The entire amplifier is made up by a aluminum chassis housing both the RF circuit and the bias circuitry. A regrlator/sequencing circuitry is used for FET biasing. The amplifier results implemented in this way show $41\pm0.3dB$ small-signal gain, 15W saturation power, a typical two tone $IM_3=-21.5dBc$ with single carrier backed off 5dB from saturation, $2^*/dBmax$ AM/PM conversion, and $3.47\pm0.25nsec$ group delay.

• KSBB Journal
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• v.17 no.2
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• pp.153-157
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• 2002

A fusion protein, consisting of a human epidermal growth factor as the recognition domain and human angiogenin as the toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as an inclusion body in recombinant E. coli, yet when the conventional solution-phase refolding process was used the refolding yield was very low due to severe aggregation, probably because of the opposite surface charge resulting from the vastly different pl values of each domain. Accordingly the solid-phase refolding process, which exploits the ionic interactions between a solid matrix and the protein, was tried, however the ionic binding yield was also very low regardless of the resins and pH conditions used. Therefore, to provide a higher affinity toward the solid matrix, six Iysine residues were tagged to the N-terminus of the hEGF domain. When cation exchange resins, such as heparin- or CM-Sepharose, were used as the matrix, the adsorption capacity increased 2.5~3-fold and the subsequent refolding yield increased nearly 15-fold compared to the conventional process. A similat result was also obtained when an Ni-NTA metal affinity resin was used.

• Clean Technology
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• v.17 no.1
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• pp.62-68
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• 2011

Hydrodynamic characteristics in multistage annular type fluidized bed (riser: $0.01{\times}0.025{\times}2.8m^3$, J-valve: $0.009{\times}0.015m^2$)were investigated. Glass beads ($d_p=101{\mu}m$, ${\rho}_b=1,590kg/m^3$, $U_{mf}=1.25{\times}10^{-2}m/s$, Geldart classification B) was used as a bed material. Accumulated weight by the electronic balance was measured to determine the solid flow rate in batch-type. In circulation condition, we measured the accumulated weight of particle transported from riser. At the steady state condition, solid circulation rate was calculated from time interval of the heated bed material passing between two thermocouples. Solid flow rate increased with increasing inlet gas velocity ($1.2-2.6U_{mf}$) and the static bed height (z, 0.24-0.68 m) from 2.2 to 23.4 kg/s. However, mean residence time decreased with increasing inlet gas velocity ($1.2-2.6U_{mf}$) and the static bed height (z, 0.24-0.68 m) from 1,438 to 440 s. The solid holdup in the riser was determined by measuring pressure differences according to the riser height. These results showed a similar trend to that of simple exponential decay type except for the top section of the riser. To verify the gas bypassing from top bubbling beds to middle bubbling beds, $CO_2$ gas was injected by tracer gas in constant ratio, and then was measured $CO_2$ concentration in outlet gas by gas chromatography. Gas bypassing occurred below 2.6% which is negligible value.

• Journal of computational fluids engineering
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• v.20 no.2
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• pp.46-51
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• 2015

In this paper, we have developed numerical model for particulated flow through narrow slit using Eulerian-Granular method. Commercial software (FLUENT) was utilized as simulation tool and main focus was to identify the effect from various numerical options for modeling of solid particles as continuos phase in granular flow. Gidaspow model was chosen as basic model for solid viscosity and drag model. And lun-et-al model was used as solid pressure and radial distribution model, respectively. Several other model options in FLUENT were tested considering the cross related effect. Mass flow rate of the particulate through the slit was measured to compare. Due to the high volume density of the stacked particulates above the slit, effect from various numerical options were not significant. The numerical results from basic model were also compared with experimental results and showed very good agreement. The effects from the characteristics of particles such as diameter, angle of internal friction, and collision coefficient were also analyzed for future design of velocity resistance layer in solar thermal absorber. Angle of internal friction was found to be the dominat variable for the particle mass flow rate considerably. More defined 3D model along with energy equation for complete solar thermal absorber design is currently underway.

• Journal of Biosystems Engineering
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• v.31 no.4 s.117
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• pp.376-384
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• 2006

This study was conducted to perform for comparative analysis of the cleaning efficiency on 3 kinds of cleaning liquid state, 3 steps of cleaning temperature $(45^{\circ}C,\;60^{\circ}C,\;70^{\circ}C,\;)$ and 6 steps of cleaning time (2.5, 5, 7.5, 10, 12.5, 15 minute). 3 kinds of cleaning liquid state are the non fluidized bed, liquid fluidized bed and liquid/solid fluidized bed. UV spectrophotometer was used in estimation of cleaning efficiency, which it is experimental equipment using the absorptiometric analysis method. Cleaning efficiency by cleaning time was increase from 2.5 minutes to 15 minutes, liquid/siolid fluidized bed was indicated the highest cleaning efficiency among the 3 kinds of cleaning liquid state and $70^{\circ}C$ of cleaning temperature at liquid/solid fluidized bed was indicated the highest cleaning efficiency as 98% among the 3 steps of cleaning temperature.

• Structural Engineering and Mechanics
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• v.57 no.2
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• pp.239-263
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• 2016

The paper studies the natural oscillation of the three-layered solid sphere with a middle layer made of Functionally Graded Material (FGM). It is assumed that the materials of the core and outer layer of the sphere are homogeneous and isotropic elastic. The three-dimensional exact equations and relations of linear elastodynamics are employed for the investigations. The discrete-analytical method proposed by the first author in his earlier works is applied for solution of the corresponding eigenvalue problem. It is assumed that the modulus of elasticity, Poisson's ratio and density of the middle-layer material vary continuously through the inward radial direction according to power law distribution. Numerical results on the natural frequencies related to the torsional and spheroidal oscillation modes are presented and discussed. In particular, it is established that the increase of the modulus of elasticity (mass density) in the inward radial direction causes an increase (a decrease) in the values of the natural frequencies.

• Corrosion Science and Technology
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• v.20 no.6
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• pp.384-390
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• 2021

In this paper, durability evaluation and surface damage mechanism were investigated through solid particle erosion (SPE) test after applying hot-dip aluminizing (HDA) technology for the purpose of maintenance of marine economizer tube. Damaged surface shape was analyzed using SEM and 3D microscope. Compositional changes and microstructure of the HDA layer were analyzed through EDS and XRD. Durability was evaluated by analyzing weight loss and surface damage depth after SPE. HDA was confirmed to have a two-layer structure of Al and Al₅Fe₂. HDA+HT was made into a single alloy layer of Al₅Fe₂ by diffusion treatment. In the microstructure of HDA+HT, void and crack defect were induced during the crystal phase transformation process. The SPE damage mechanism depends on material properties. Plastic deformation occurred in the substrate and HDA due to ductility, whereas weight loss due to brittleness occurred significantly in HDA+HT. As a result, the substrate and HDA showed better SPE resistance than HDA+HT.

• Journal of the Korean Society of Propulsion Engineers
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• v.25 no.4
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• pp.12-18
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• 2021

This study aimed at preparing the solid propellants featuring high pressure exponent available for throttleable rocket system development. The combustion properties of solid propellant based on PCP as a prepolymer were investigated with the different nitramine oxidizer, HMX and HNIW. As a main oxidizer, HNIW could deliver higher burning rate, specific impulse and flame temperature than HMX. In addition, the introduction of AP as a co-oxidizer in PCP/Nitramine propellants could enhance burning rate, specific impulse and flame temperature, showing the lower pressure exponent with increasing the content of fine-sized AP, total solids and plasticizer. Moreover, we examined the temperature sensitivity on burning rate of propellants between 150 psia and 2,500 psia.