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Culture of Rat Salivary Acinar Cells (백서 타액선 선포 세포의 배양)

  • Lee, Sung-Woo;Han, Song;Kho, Hong-Seop
    • Journal of Oral Medicine and Pain
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    • v.24 no.2
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    • pp.163-169
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    • 1999
  • We investigated the culture condition and effects of various growth factors on the culture of salivary gland acinar cells. Male, Sprague-Dawley rats (about 6 weeks old) were sacrificed and their submandibular, sublingual, and parotid glands were used as specimens. High oxygen level more than 90% and coating of Matrigel on culture dish were important factors to help increase the survival time of acinar cells, Proper concentration of enzymes such as collagenase and hyaluronidase during isolation steps was also important. Addition of various growth factors such as dexamethasone, insulin, transferrin, selenous acid, reduced glutathione, epidermal growth factor, isoproterenol, and putrescine in culture medium helped to increase lifetime of cultured salivary acinar cells.

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The Effects of Onomatopoeia and Mimetic Word Productive Training Program on Auditory Performance and Vocal Development in Children with Cochlear Implants (의성어.의태어 산출 프로그램이 인공와우 착용 아동의 청능 및 발성 발달에 미치는 효과)

  • Kim, Yu-Kyung;Seok, Dong-Il
    • Speech Sciences
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    • v.11 no.2
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    • pp.51-67
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    • 2004
  • The objective of this study was to investigate the effects in auditory performance and vocal development of Onomatopoeia and Mimetic Word Productive Training Program in prelingually deafened children with cochlear implantation. The effects were measured with Lip-profile (Listening progress profile: LiP), the number of utterances, vocal developmental level and phonetic inventory. Subjects were four children with cochlear implants who were able to detect speech sounds and environmental sounds. The Onomatopoeia and Mimetic word Productive Training Program was made up of 3 steps with 24 Onomatopoeia and Mimetic words. This study was pre and post design. The results of the study were as follows: First, after Onomatopoeia and Mimetic word Productive Training Program was treated, LiP score was significantly higher. Second, after this program was treated, the number of utterances and emergence of both canonical and postcanonical utterances were increased. Emergence of vowel and consonant Features were increased and diversified. In conclusion, Onomatopoeia and Mimetic Word Productive Training Program appeared to facilitate efficient auditory performance and vocal development.

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Pronunciation Lexicon Optimization with Applying Variant Selection Criteria (발음 변이의 발음사전 포함 결정 조건을 통한 발음사전 최적화)

  • Jeon, Je-Hun;Chung, Min-Hwa
    • Proceedings of the KSPS conference
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    • 2006.11a
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    • pp.24-27
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    • 2006
  • This paper describes how a domain dependent pronunciation lexicon is generated and optimized for Korean large vocabulary continuous speech recognition(LVCSR). At the level of lexicon, pronunciation variations are usually modeled by adding pronunciation variants to the lexicon. We propose the criteria for selecting appropriate pronunciation variants in lexicon: (i) likelihood and (ii) frequency factors to select variants. Our experiment is conducted in three steps. First, the variants are generated with knowledge-based rules. Second, we generate a domain dependent lexicon which includes various numbers of pronunciation variants based on the proposed criteria. Finally, the WERs and RTFs are examined with each lexicon. In the experiment, 0.72% WER reduction is obtained by introducing the variants pruning criteria. Furthermore, RTF is not deteriorated although the average number of variants is higher than that of compared lexica.

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A Study on 3-D Shape Measurement and Application by Using Digital Projection Moire (II) (디지털 영사식 무아레를 이용한 3차원 형상 측정과 응용에 관한 연구(II))

  • Ryu, Weon-Jae;Kang, Young-June;Rho, Hyung-Min;Lee, Dong-Hwan
    • Journal of the Korean Society for Precision Engineering
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    • v.24 no.5
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    • pp.62-67
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    • 2007
  • A simple dimension measuring method for the measurement of human bust has been developed using projection moire. The 3-D data of a human bust was calculated from the 2dimensional image information obtained by the stripe using projection moire. The creation of 3-D geometric shape by digitizing real objects has been widely investigated in reverse engineering(RE). This procedure generally consists of three basic steps: data capture, data alignment and model reconstruction. In order to achieve a complete model, multiple scans must be taken and aligned.

Skeletal Development - Wnts Are in Control

  • Hartmann, Christine
    • Molecules and Cells
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    • v.24 no.2
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    • pp.177-184
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    • 2007
  • Approximately 200 individual skeletal elements, which differ in shape and size, are the building blocks of the vertebrate skeleton. Various features of the individual skeletal elements, such as their location, shape, growth and differentiation rate, are being determined during embryonic development. A few skeletal elements, such as the lateral halves of the clavicle and parts of the skull are formed by a process called intramembranous ossification, whereby mesenchymal cells differentiate directly into osteoblasts, while the majority of skeletal elements are formed via endochondral ossification. The latter process starts with the formation of a cartilaginous template, which eventually is being replaced by bone. This requires co-regulation of differentiation of the cell-types specific for cartilage and bone, chondrocytes and osteoblasts, respectively. In recent years it has been demonstrated that Wnt family members and their respective intracellular pathways, such as non-canonical and the canonical $Wnt/{\beta}$-catenin pathway, play important and diverse roles during different steps of vertebrate skeletal development. Based on the recent discoveries modulation of the canonical Wnt-signaling pathway could be an interesting approach to direct stem cells into certain skeletal lineages.

The effect by taping therapy for pain of knee joint (슬관절 통증에 대한 테이핑요법의 효과)

  • Yang, Kyung-Han;Han, Jong-Man;Kim, Su-Han
    • Journal of Korean Physical Therapy Science
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    • v.13 no.4
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    • pp.35-41
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    • 2006
  • The Purpose : This study was to figure out the alleviation of the pain of knee joint using Taping Therapy Diagram developed 6 steps treatments. Method : 30 old patients who live in 3 different old people's homes in D place had been treated for three months - twice a week, total 8 times- from 1, September to 30, November 2006. Result : After the pre and post taping therapy treatments, the patient's pain degree was decreased from 5.23 _+1.24 to 3.20 _+ 1.06. Conclusion : The result show that it based on taping therapy Diagram of arikawa was effective on the pain of knee joint.

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A B-spline based Branch & Bound Algorithm for Global Optimization (전역 최적화를 위한 B-스플라인 기반의 Branch & Bound알고리즘)

  • Park, Sang-Kun
    • Korean Journal of Computational Design and Engineering
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    • v.15 no.1
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    • pp.24-32
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    • 2010
  • This paper introduces a B-spline based branch & bound algorithm for global optimization. The branch & bound is a well-known algorithm paradigm for global optimization, of which key components are the subdivision scheme and the bound calculation scheme. For this, we consider the B-spline hypervolume to approximate an objective function defined in a design space. This model enables us to subdivide the design space, and to compute the upper & lower bound of each subspace where the bound calculation is based on the LHS sampling points. We also describe a search tree to represent the searching process for optimal solution, and explain iteration steps and some conditions necessary to carry out the algorithm. Finally, the performance of the proposed algorithm is examined on some test problems which would cover most difficulties faced in global optimization area. It shows that the proposed algorithm is complete algorithm not using heuristics, provides an approximate global solution within prescribed tolerances, and has the good possibility for large scale NP-hard optimization.

Flexural Behavior of PSC Beam Using High Strength Concrete (고강도 PSC BEAM 교량의 휨거동)

  • 정원기;이형준;이규정;윤석구;한승환;김기수
    • Proceedings of the Korea Concrete Institute Conference
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    • 1998.10b
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    • pp.706-711
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    • 1998
  • Structural tests of the PSC Beam bridge using high strength concrete, concrete compressive strength 700kg/$\textrm{cm}^2$, are conducted for the application including durability and serviceability of the bridge. Current design safety factors with respect to the jacking force and the service design load DB-24 are applied to the design of the bridge. Concrete compressive strength 700kg/$\textrm{cm}^2$, girder depth 2.3m, girder space 3.2m, span length 20m, and slab thickness 27cm are selected for the bridge test. The Bulb-Tee section of the girders is applied instead of I section because it is well known more stable to the longer span(40m). Static load test(4 beams) with composite and non-composite section, and fatigue load test(1 beams) with composite section are conducted. Crack moment, ultimate load, deflections with load steps, and strains of the beam section for those bridges are investigated. The structural test results of the bridges showed a good performance for a safety and a serviceability.

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A Study For the Development of Enhanced Classification Method of Consumer Attributes (사용자 요구품질 추출과 분류방법의 개선에 관한 연구)

  • 김승남;김철홍;정영배;김연수
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.24 no.67
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    • pp.77-82
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    • 2001
  • A study was conducted to develop a better classification method of Consumer Attributes that can enhance user-centered product design process. A modified QFD(Quality Function Deployment) survey form based upon Fuzzy set theory was proposed which contains 9 steps of importance level, and Certainty and Necessity function to improve the reliability of extracted consumer attributes. To verify the betterment and advantage of proposed classification method, a series of questionnaire survey was performed. Thirty male and 30 female university students were participated in the survey using a VCR as a target product. The result of the study showed that 80% of subjects were preferred the proposed classification over existing method. A cluster analysis was performed to further verify the betterment of the proposed method. The result also supported that the proposed classification method is more reliable and enhanced method in extracting consumer attributes and can be applied in the product design.

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A Construction Theory of Multiple-Valued Logic Sequential Machines on $GF(2^M)$

  • 박춘명;김흥수
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.24 no.5
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    • pp.823-832
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    • 1987
  • This pper presents a method for constructing multiple-valued logic sequential machines based on Galois field. First, we assign all elements in GF(2**m) to bit codes using mathematical properties of GF(2**m). Then, we realized the sequencial machine circuits with and withoutm feed-back. 1) Sequential machines with feed-back are constructed by using only MUX from state-transition diagram expressing the information of sequential machines. 2) Sequential machines without feed-back are constructed by following steps. First, we assigned states in state-transition disgram to state bit codes, then obtained state function and predecessor table explaining the relationship between present states and previous states. Next, we obtained next-state function from state function and predecessor table. Finally we realized the circuit using MUX and decoder.

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