• Proceedings of the Ginseng society Conference
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• 1980.09a
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• pp.87-113
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• 1980

This experiment was carried out to evaluate the effects of Korean ginseng extract on carcinogenesis induced by various chemical carcinogens. Red ginseng extract was used for this study and was administered orally to the experimental animals. Carcinogens that were injected in subscapsular region of ICR newborn mice within 24 hours after birth were 9,10-dimethyl-1,2-benzan-thracene (DMBA), urethane, N-2-fluorenylacetamide(AAF), aflatoxin $B_1$ and tobacco smoke condensate. N -methyl-N -nitroso-N'-nitroguani-dine(MNNG) was injected subcutaneously at the back of wistar rats. Experimental animals were autopsied in immediately after being sacrificed. All major organs were examined grossly and weighted. After fixation histopathological preparations were made for microscopical study. Following results were obtained. In DMBA group sacrificed at the 26th week after the treatment with DMBA, the incidence of lung adenoma was $77\%$ and the average number of the tumor was 17. However, in DMBA combined with red ginseng group, the incidence was $78\%$ and the average number of lung adenoma was 14.1. This indicates that ginseng extract had no effect on the incidence of lung adenoma but decreased the average number of lung adenoma by $17\%.$ In DMBA group sacrificed at the 48th week after the injection of DMBA, the lung adenoma incidence was $88\%.$ The average diameter of the largest lung adenoma was 3.5 cm, the incidence of diffuse pulmonary infiltration was $18\%$ and the average lung weight of male experimental mice was $528.2{\pm}469.1\;gm.$ On the other hand, in DMBA combined with red ginseng group sacrificed at the 48th week, the incidence of lung adenoma was $96\%.$ The average diameter of the largest adenoma was 2.7 cm, the incidence of diffuse pulmonary infiltration was $7\%$ and the average lung weight of male mice was $418.0{\pm}520\;gm.$ These observations show that ginseng extract did not have any inhibitory effect on the incidence of lung adenoma but decreased the average diameter of the largest lung adenoma by $23\%,$ the incidence of duffuse pulmonary infiltration by $63\%$ and the average lung weight of male experimental mice by $21\%.$ From these results we have found that the prolonged administration with ginseng extract showed no inhibitory effect on the incidence of adenoma but it had the inhibitory effect on the proliferation of lung adenomas induced by DMBA. In urethane group sacrificed at the 28th week after the injection of urethane, the incidence of lung adenoma was $94\%$ and the average number of lung adenoma was 8.6. In urethane combined with red ginseng group, the. incidence of lung adenoma was $73\%$ and the average number of adenoma was 6.0. These results indicate that there were $22\%$ decrease of the lung adenoma incidence and $31\%$ decrease of the average number of adenoma in urethane combined with red ginseng group. And in urethane group sacrificed at the 50th week, the incidence of lung adenoma was $98\%$ and the incidence of diffuse pulmonary infiltration was $14\%$. In urethane combined with ginseng group the incidence of lung adenoma was $85\%$ and the incidence of diffuse pulmonary infiltration was $12\%$. Therefore the ginseng administration resulted in $15\%$ decrease of the lung adenoma incidence and $14\%$ decrease of the diffuse pulmonary infiltration incidence. From these results we knew that the prolonged administration with ginseng extract inhibited the incidence and also the proliferation of the lung adenoma induced by urethane. Lung adenoma and hepatoma were induced in the experimental mice sacrificed at the 68th week but not in the experimental mice sacrificed at the 28th week after the injection of AAF. In AAF group sacrificed at the 68th week after the injection of AAF the incidence of lung adenoma was $18\%$ and the incidence of hepatoma was $27\%$. And in AAF combined with ginseng group the lung adenoma incidence was $12\%$ and the hepatoma incidence was $37\%$. So the ginseng seemed to decrease the lung adenoma incidence by AAF, but we were unable to conclude the significant inhibitory effect of the ginseng extract on the incidence of lung adenoma by AAF because the above incidence of lung adenoma were similar to that of control group which was $11\%$. And these experimental data revealed that ginseng extract didn't have any inhibitory effect on the incidence of hepatoma induced by AAF. In aflatoxin $B_1$ group sacrificed at the 56th week, the incidence of lung adenoma was $24\%$ and hepatoma was $11\%$. However in aflatoxin $B_1$ combined with ginseng group the incidence of lung adenoma was $17\%$ and hepatoma was $3\%$ These results indicate that there were $29\%$ decrease of the lung adenoma incidence and $75\%$ decrease of the hepatoma incidence in aflatoxin $B_1$ combined with ginseng group. In tobacco smoke condensate experimental group sacrificed at 67th week, no tumors were induced except just a few lung adenoma. The lung adenoma incidence both in tobacco smoke condensate group and in tobacco smoke condensate combined with ginseng group was $8\%$. And this incidence rate was similar to that of control group. These results indicate that the injection of 320 ug tobacco smoke condensate per ICR newborn mouse was unable to induce lung adenoma in our experiments. In MNNG group sacrificed at the 27th week the tumor incidence was $38.5\%$ and in MNNG combined with ginseng extract group was $37\%$. In MNNG group for investigation of the life span of tumor bearing rats the tumor incidence was $93\%$ and the average life span of tumor bearing rats was 318 days. And in MNNG combined with ginseng extract group the tumor incidence was $96\%$ and the average life span was 337 days. Tumor induced by MNNG was almost sarcoma. This indicates that there was no inhibitory effect of ginseng extract on the tumor incidence, but the extract prolonged the average life span of tumor bearing rats by approximately 19 days.

• Korean Journal of Ichthyology
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• v.1 no.1_2
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• pp.9-18
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• 1989

Blenniid fish, Istiblennius stellifer(jordan et Snyder) is distributed in the coastal waters of south-eastern Korea and Japan. Matured adults of blenniid fish were collected from the rocky shore of Namchun-dong, Nam-gu, Pusan, Korea on May 15, 1988. The fertilized eggs were incubated and the larvae were reared in laboratory. The eggs of this species were demersal and adhesive, and their diameters varied from 0.84 to 0.88 mm(mean 0.86 mm, n=30). They have a number of small oil globules. The water temperature throughout incubation ranged from 18.5 to $23.3^{\circ}C$ and salinity was maintained at $28.2-29.5\;^{\circ}/_{\circ\circ}$. The hatching took place in 130 hours after fertilization. The newly hatched larvae were 2.70 mm in total length with 11 (abdominal)+22~25 (caudal)=33-36 myomeres. The larvae absorbed the yolk material and oil globule completely in 10 days after hatching and became postlarvae. Total lengths of the larvae reached 4.65 and 5.75 mm in 10 and 13 days after the hatching, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.197-204
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• 1989

To evaluate the effect of soil improvement on the chemical properties and corn silage yield, this experiment was carried out in the newly-reclaimed sloped land. Corn (Suwon 19) was cultivated under the six different treatments including integrated improvement plot at Songjeong loam, 20 percent slope, from 1985 to 1987 and various soil chemical properties and silage yield were investigated. pH of topsoil was above 5.0 except for control and phosphate plot, but lime and integrated improvement plots were above 5.0 in subsoil. The contents of organic matter of topsoil were above 2.0% except control and subsoiling plot. Except control, the range of cation exchange capacity was 7.4-7.8 me/100g in topsoil, 7.0-7.7 me/100g in subsoil. Soil bacteria density of root zone was the highest in integrated improvement than the other treatments, and it was higher at the harvesting stage than the heading stage. Mean density of microorganism was $61.3{\times}10^5$ in bacteria, $21.5{\times}10^4$ in fungi and actinomycetes was B/F ratio was 28.5 and B/A ratio was 2.9. Vertical root distribution of total and 10cm depth below was more in the integrated improvement and subsoi ling plot than the other plots. Total nitrogen (T-N) content was higher in integrated improvement plot, and phosphate content of leaves was higher in compost and integrated improvement plot, but stem and grain were not different. Potassium content of the plant was higher in integra ted improvement plot. Correlation between dry matter yield and T-N was more significant than the other elements. And the contents of phosphate, calcium and magnesium were significant at 5%, but potassium was not.

• Journal of Environmental Science International
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• v.22 no.9
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• pp.1181-1186
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• 2013

Due to climate change, the occurrence of abnormal weather conditions has become more frequent, causing damage to vegetable crops grown in Korea. Hot pepper, Chinese cabbage and radish, the three most popular vegetables in Korea, are produced more in the field than in the greenhouse. It has been a trend that the time for field transplanting of seedlings is getting earlier and earlier as the spring temperatures keep rising. Seedlings transplanted too early in the spring take a longer time to resume the normal growth, because they are exposed to suboptimal temperature conditions. This study examined the influence of air temperature during seedling growth on the time required to reach the first fruit maturity and yield of hot pepper. Seedling plants of 'Super Manita' hot pepper was grown in temperatures $2.5^{\circ}C$ and $5.0^{\circ}C$ lower than the optimum temperature (determined by the average of temperatures for the past 5 years). Seedlings were transplanted into round plastic containers (30-cm diam., 45-cm height) and were placed in growth chambers in which the ambient temperature was controlled under natural sunlight. The growth of seedlings under lowered temperatures was reduced compared to the control. The mineral (K, Mg, P, N) concentrations in the leaf tissues were higher when plants were grown with the ambient temperature $2.5^{\circ}C$ lower than the optimum, regardless of changes in other growth parameters. Tissue calcium (Ca) concentration was the highest in the plants grown with optimum temperature. The carbohydrate to nitrogen (C/N) ratio, which was the highest (18.3) in the plants grown with optimum temperature, decreased concomitantly as the ambient temperature was lowered $2.5^{\circ}C$ and $5.0^{\circ}C$. The yield of the early harvested fruits was also reduced as the ambient temperature became lower. The first fruit harvest date for the plants grown with optimum temperature (June 27) was 13 days and 40 days, respectively, earlier than that in plants grown with $2.5^{\circ}C$ (July 10) and $5.0^{\circ}C$ (Aug 6) lower ambient temperatures. The fruit yield per plant for the optimum temperature (724 g) was the greatest compared to those grown with $2.5^{\circ}C$ (446 g) and $5.0^{\circ}C$ (236 g) lower temperatures. The result of this study suggests that the growers should be cautioned not to transplant their hot pepper seedlings too early into the field, since it may delay the time of first harvest eventually reducing total fruit yield.

• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.2
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• pp.57-67
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• 2020

Pinus densiflora is the most widely distributed tree species in South Korea. Its ecological and socio-cultural attributes makes it one of the most important tree species in S. Korea. In recent times however, the distribution of P. densiflora has been affected by dieback. This phenomenon has largely been attributed to climate change. This study was conducted to investigate the responses of growth and physiology of P. densiflora to drought and nitrogen fertiliz ation according to the RCP 8.5 scenario. A Temperature Gradient Chamber (TGC) and CO₂. Temperature Gradient Chamber (CTGC) were used to simulate climate change conditions. The treatments were established with temperature (control versus +3 and +5℃; aCeT) and CO₂ (control: aCaT versus x1.6 and x2.2; eCeT), watering(control versus drought), fertilization(control versus fertilized). Net photosynthesis (P_n), stomatal conductance (g_s), biomass and relative soil volumetric water content (VWC) were measured to examine physiological responses and growth. Relative soil VWC in aCeT significantly decreased after the onset of drought. P_n and g_s in both aCeT and eCeT with fertiliz ation were high before drought but decreased rapidly after 7 days under drought because nitrogen fertilization effect did not last long. The fastest mortality was 46 days in aCeT and the longest survival was 56 days in eCeT after the onset of drought. Total and partial biomass (leaf, stem and root) in both aCeT and eCeT with fertiliz ation were significantly high, but significantly low in aCeT. The results of the study are helpful in addressing P. densiflora vulnerability to climate change by highlighting physiological responses related to carbon allocation under differing simulated environmental stressors.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.5 no.1
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• pp.47-55
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• 1999

Applications of chitosan are related to molecular weight and degree of deacetylation(DOD) of chitosan completely. The molecular weight and DOD were greatly affected by the concentration of solution time and temperature. The degree of demineralization was not significantly different at $50^{\circ}C\;and\;70^{\circ}C$ after 30 minutes. Deproteinization decreased as process time increased. The nitrogen content was reached to 6.92% after 90 minute at $80^{\circ}C$, which is similar to theoretical nitrogen content of chitin. The DOD was 82.84% after 2 hours reaction and increased as the reaction time increased in the process. Viscosity and molecular weight are increased as recycling number of concentrated NaOH solution increased. Chemical, biological and physical properties of chitosan depend on the DOD and molecular size of the molecule. Tensile strength of the films from acetic acid solutions was between $28.9{\sim}33.6$ MPa and was generally higher than that of the films from lactic acid. Elongation of the films from lactic acid was between $97.0{\sim}109.7%$ and was generally higher than that of the films from the acetic acid. Water vapor permeability of the films prepared from lcetic acid solutions was between $1.9{\sim}2.3ng{\cdot}m/m^2{\cdot}s{\cdot}Pa$ and was generally higher than that of the films from the acetic acid.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.6
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• pp.918-926
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• 2008

It is well known non-drug therapy for hypertension patients can reduce blood pressure. These types of therapy include maintaining ones ideal body weight, quitting smoking, consuming large amounts of fruits and vegetables consuming low levels of saturated fat and salt and regular exercise. Fermented milk protein derived biologically active peptides such as isoleucine-proline-proline_(IPP) or valine-proline-proline_(VPP) have been shown to lower blood pressure in hypertensive subjects. This study was conducted to investigate the antihypertensive effects of medical nutritional therapy _(MNT) in accordance with the consumption of fermented milk enriched with IPP and VPP. To accomplish this, we conducted a randomized case-controlled study of 43 subjects who had blood pressure levels greater than 120/80 mmHg. The subjects in the study group were randomly allocated into two groups, an MNT + fermented milk (100mL/day) group (n=21) and an MNT+L. helveticus fermented milk with tripeptides (IPP=2.2mg, VPP=2.6mg/100mL) group (n=22). The MNT included weight management, reduction of sodium, total fat and saturated fat intake, increased intake of fruits and vegetables, and increased intake of low fat dairy products. The treatments were administered for 12 weeks during which time no drug interventions were conducted. The daily intakes of total calories, fats, cholesterol and Na decreased significantly after 12 weeks of MNT in the control and the experimental groups. In addition, the systolic blood pressure de creased significantly in the control and experimental groups; however, the diastolic blood pressure only decreased significantly in the experimental group. Overall, the results of this study indicate that the intake of fermented milk containing IPP and VPP in conjunction with MNT exerted positive effects on the blood pressure of pre- and hypertensive subjects.

• Journal of dental hygiene science
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• v.14 no.1
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• pp.22-28
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• 2014

This research aimed to understand the relevance between irritable bowel syndrome (IBS) and sleep quality for dental hygiene students in certain areas. It was conducted on 344 students from four universities in Jeollabuk-do from May to September 2013 using a structured questionnaire. The following are the results. 57.6% of the IBS bowel movement conditions were mixed type, 10.5% constipation type, 7.6% uncategorized, and 6.1% diarrhea type. For sleep quality based on the subjects' general characteristics, third years were 11.45, second years 10.90, and first years 9.53 (p=0.034). The sleep quality was statistically significantly lower as the years increased, and even for IBS (p=0.026), it was low and showed a significant difference. For difference in sleep quality based on IBS presence, habitual sleep efficiency, sleeping pill dosage (p=0.043), and day time functional disorder (p=0.007) showed statistically significant differences and lower sleep quality than the control group. For difference in sleep quality based on IBS bowel movement condition, mixed type was the highest for sleep disturbance at 6.86, constipation type was 1.00 for habitual sleep efficiency, constipation type was 1.42 for subjective sleep quality, uncategorized type was 0.15 for sleeping pill dosage but not statistically significant. In day time functional disorder, constipation type was the highest at 2.61 and showed a significant difference (p=0.012). The correlation between the sub-factors of sleep quality was positive. Based on the above study results, we learned that for IBS, sleep quality becomes lower as the year increases, and functional disorder was observed during day time due to low sleep quality. Education on IBS symptoms is needed, adequate stress management method to alleviate symptoms and prevention programs for correct diet must be developed to enhance sleep quality.

• Radiation Oncology Journal
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• v.22 no.2
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• pp.115-123
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• 2004

Purpose : This retrospective study was conducted to compare early preliminary results of breast conservation therapy (BCT) with mastectomy In early breast cancer. Materials and Methods : We evaluated 171 women with AJCC stage I and II breast cancer who had been treated at Kangnam St. Mary's Hospital from March 1989 to August 1996. Eighty-eight patients underwent mastectomy and 85 patients did conservative surgery with breast irradiation. in the BCT group, all patients received whole breast irradiation to a total dose of 45$\~$50 Gy/5$\~$6 wks, followed by a boost to the original tumor site at least 60 Gy. Chemotherapy was administered to 29 (34.1$\%$) patients in BCT and 40 (45.5$\%$) in mastectomy, with various sequencing of surgery and/or radiation. We compared survival rate, patterns of failure in each treatment group and the prognostic factors that had a significant effect on treatment failure. The median follow-up time was 63 months (19$\~$111 months). Log rank test was used to estimate the prognostic factors for treatment failure. Results : Overall survival, disease free survival, locoregional recurrence and distant metastasis rates were not significantly different between the two treatment groups. During the follow-up period, 11 patients (12.5$\%$)in the mastectomy group and 10 patients (11.8%$\%$ in the BCT group were failed. Six local recurrences occurred after mastectomy and 5 after BCT Five patients fatted at distant site in mastectomy and 4 in BCT. Of the local recurrence cases, five of 6 mastectomy patients and 3 of S BCT patients were alive with no evidence of disease after salvage surgery and/or chemoirradiation. Our results indicated that the major influence on survival was distant metastasis. Unfortunately, control of distant metastasisis was not frequently achieved. Even with salvage systemic therapy or radiotherapy, most of distant metastasis patients died or had uncontrolled disease in both treatment groups: only one of 4 BCT patients and none of mastectomy patients were alive without disease. There was no apparent difference in the incidence rate of contralateral breast cancer and non-breast 2$^{nd}$ primary tumor between the two treatment groups. Univariate Log-rank test identified the N stage and the involved axillary LN number as distinct prognostic factors that were highly predictive of treatment failure in both treatment groups. Additionally, marginal status in BCT and histologic nuclear grade In the mastectomy group were risk factors for treatment fallure (p < 0.05). Concousion : Although further careful follow-up is necessary to confirm the trends evident In this serles, it would appear that patterns of failure and survival rate following conservative surgery and radiotherapy in early breast cancer are similar to those following mastectomy. The great majority of patients with local recurrence had an exellent salvage rate in both treatment groups. Therefore, these preliminary short term results support BCT as an equally effective management for early breast cancer as an alternative to mastectomy.

• Journal of Periodontal and Implant Science
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• v.28 no.4
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• pp.645-660
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• 1998

The purpose of this study was to make and ascertain a decision making process on the base of patient-oriented utilitarianism in the treatment of patients of chronic adult periodontitis. Fifty subjects were chosen in Yonsei Dental hospital and the other fifty were chosen in Severance dental hospital according to the selection criteria. Fifty four patients agreed in this study. NS group(N=32) was treated with scaling and root planing without any surgical intervention, the other S group(N=22) done with flap operation. During the active treatment and healing time, all patients of both groups were educated about the importance of oral hygiene and controlled every visit to the hospital. When periodontal treatment needed according to the diagnostic results, some patients were subjected to professional tooth cleaning and scaling once every 3 months according to an individually designed oral hygienic protocol. Probing depth was recorded on baseline and 18 months after treatments. A questionnaire composed of 6 kinds(hygienic easiness, hypersensitivity, post treatment comfort, complication, functional comfort, compliance) of questions was delivered to each patient to obtain the subjective evaluation regarding the results of therapy. The decision tree for the treatment of adult periodontal disease was made on the result of 2 kinds of periodontal treatment and patient's ubjective evaluation. The optimal path was calculated by using the success rate of the results as the probability and utility according to relative value and the economic value in the insurance system. The success rate to achieve the diagnostic goal of periodontal treatment as the remaining pocket depth less than 3mm and without BOP was $0.83{\pm}0.12$ by non surgical treatment and $0.82{\pm}0.14$ by surgical treatment without any statistically significant difference. The moderate success rate of more than 4mm probing pocket depth were 0.17 together. The utilities of non-surgical treatment results were 100 for a result with less than 3mm probing pocket depth, 80 for the other results with more than 4mm probing pocket depth, 0 for the extraction. Those of surgical treatment results were the same except 75 for the results with more than 4mm. The pooling results of subjective evaluation by using a questionnaire were 60% for satisfaction level and 40% for no satisfaction level in the patient group receiving nonsurgical treatment and 33% and 67% in the other group receiving surgical treatment. The utilities for 4 satisfaction levels were 100, 75, 60, 50 on the base of that the patient would express the satisfaction level with normal distribution. The optimal path of periodontal treatment was rolled back by timing the utility on terminal node and the success rate, the distributed ratio of patient's satisfaction level. Both results of the calculation was non surgical treatment. Therefore, it can be said that non-surgical treatment may be the optimal path for this decision tree of treatment protocol if the goal of the periodontal treatment is to achieve the remaining probing pocket depth of less than 3mm for adult chronic periodontitis and if the utilitarian philosophy to maximise the expected utility for the patients is advocated.